scholarly journals Effect of Emodin on Preventing Postoperative Intra-Abdominal Adhesion Formation

2017 ◽  
Vol 2017 ◽  
pp. 1-12 ◽  
Author(s):  
Guangbing Wei ◽  
Yunhua Wu ◽  
Qi Gao ◽  
Cancan Zhou ◽  
Kai Wang ◽  
...  
Keyword(s):  
Adhesion Formation ◽  
Major Complication ◽  
Chinese Herbs ◽  
Postoperative Adhesion ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Abdominal Adhesions ◽  
Abdominal Adhesion ◽  
Naturally Occurring ◽  
Oral Doses

Background. Postoperative intra-abdominal adhesions are a major complication after abdominal surgery. Although various methods have been used to prevent and treat adhesions, the effects have not been satisfactory. Emodin, a naturally occurring anthraquinone derivative and an active ingredient in traditional Chinese herbs, exhibits a variety of pharmacological effects. In our study, we demonstrated the effect of emodin treatment on preventing postoperative adhesion formation. Materials and Methods. A total of 48 rats were divided into six groups. Abdominal adhesions were created by abrasion of the cecum and its opposite abdominal wall. In the experimental groups, the rats were administered daily oral doses of emodin. On the seventh day after operation, the rats were euthanized, and blood and pathological specimens were collected. Abdominal adhesion formation was evaluated by necropsy, pathology, immunohistochemistry, Western blot, and enzyme-linked immunosorbent assay analyses. Results. Abdominal adhesions were markedly reduced by emodin treatment. Compared with the control group, collagen deposition was reduced and the peritoneal mesothelial completeness rate was higher in the emodin-treated groups. Emodin had anti-inflammatory effects, reduced oxidative stress, and promoted the movement of the intestinal tract (P<0.05). Conclusion. Emodin significantly reduced intra-abdominal adhesion formation in a rat model.

scholarly journals Preventive Effects of the Intestine Function Recovery Decoction, a Traditional Chinese Medicine, on Postoperative Intra-Abdominal Adhesion Formation in a Rat Model

2016 ◽  
Vol 2016 ◽  
pp. 1-10 ◽  
Author(s):  
Cancan Zhou ◽  
Pengbo Jia ◽  
Zhengdong Jiang ◽  
Ke Chen ◽  
Guanghui Wang ◽  
...  
Keyword(s):  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Rat Model ◽  
Adhesion Formation ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
High Dose ◽  
Abdominal Adhesion ◽  
Function Recovery ◽  
Model Control

The intestine function recovery decoction (IFRD) is a traditional Chinese medicine that has been used for the treatment of adhesive intestinal obstruction. In this study, the preventative effects and probable mechanism of the IFRD were investigated in a rat model. We randomly assigned rats to five groups: normal, model, control, low dose IFRD, and high dose IFRD. In the animal model, the caecum wall and parietal peritoneum were abraded to induce intra-abdominal adhesion formation. Seven days after surgery, adhesion scores were assessed using a visual scoring system, and histopathological samples were examined. The levels of serum interleukin-6 (IL-6) and transforming growth factor beta-1 (TGF-β1) were analysed by an enzyme-linked immunosorbent assay (ELISA). The results showed that a high dose of IFRD reduced the grade of intra-abdominal adhesion in rats. Furthermore, the grades of inflammation, fibrosis, and neovascularization in the high dose IFRD group were significantly lower than those in the control group. The results indicate that the IFRD can prevent intra-abdominal adhesion formation in a rat model. These data suggest that the IFRD may be an effective antiadhesion agent.

scholarly journals Effect of Resveratrol on the Prevention of Intra-Abdominal Adhesion Formation in a Rat Model

2016 ◽  
Vol 39 (1) ◽  
pp. 33-46 ◽  
Author(s):  
Guangbing Wei ◽  
Xin Chen ◽  
Guanghui Wang ◽  
Lin Fan ◽  
Kang Wang ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Rat Model ◽  
Transforming Growth Factor ◽  
Adhesion Formation ◽  
Adhesion Prevention ◽  
Enzyme Linked Immunosorbent Assay ◽  
Abdominal Adhesions ◽  
Abdominal Adhesion ◽  
Tnf Α ◽  
Tgf Β1

Background: Intra-abdominal adhesions are a very common complication following abdominal surgery. Our previous studies have demonstrated that the inhibition of inflammation at the sites of peritoneal injury can prevent the formation of intra-abdominal adhesions. Resveratrol is a natural extract with a broad range of anti-inflammatory effects. Therefore, we propose that resveratrol can reduce the formation of intra-abdominal adhesions after surgery. The aim of this study was to investigate the effect of resveratrol on intra-abdominal adhesion prevention in a rat model with surgery-induced peritoneal adhesions. Materials and Methods: The cecum wall and its opposite parietal peritoneum were abraded following laparotomy to induce intra-abdominal adhesion formation. Varying doses of resveratrol were administered to the animals. On the eighth day after surgery, the adhesion score was assessed using a visual scoring system. Picrosirius red staining and a hydroxyproline assay were used to assess the amount of collagen deposition in the adhesion tissues. The levels of serum interleukin-6 (IL-6), tumor necrosis factor (TNF-α), and transforming growth factor beta-1 (TGF-β1) were determined by an enzyme-linked immunosorbent assay (ELISA). Western blotting was performed to determine the protein expression of TGF-β1, fibrinogen, and α-smooth muscle actin (α-SMA) in rat peritoneal adhesion tissue. Real-time RT-PCR was performed to quantify the mRNA expression of TGF-β1, fibrinogen, and α-SMA. Results: Resveratrol significantly reduced intra-abdominal adhesion formation and fibrin deposition in the rat model. Furthermore, resveratrol significantly reduced the serum levels of IL-6, TNF-α, and TGF-β1. The protein and mRNA expression of TGF-β1, fibrinogen, and α-SMA in the rat peritoneum and adhesion tissues were also down-regulated due to resveratrol intervention. Conclusion: Resveratrol can effectively prevent the formation of postoperative intra-abdominal adhesions in a rat model. This effect may be related to the suppression of inflammatory cytokine expression in the injured peritoneum by resveratrol. This study suggests that resveratrol may be a new and effective anti-adhesive agent that is worthy of further study and has potential application value.

scholarly journals Comparison of Honey versus Polylactide Anti-Adhesion Barrier on Peritoneal Adhesion and Healing of Colon Anastomosis in Rabbits

2019 ◽  
Vol 7 (10) ◽  
pp. 1597-1601 ◽  
Author(s):  
Ali Reza Negahi ◽  
Parisa Hosseinpour ◽  
Mohammad Vaziri ◽  
Hamed Vaseghi ◽  
Pedram Darvish ◽  
...  
Keyword(s):  
Intestinal Anastomosis ◽  
Adhesion Formation ◽  
Postoperative Adhesion ◽  
Control Group ◽  
Colon Anastomosis ◽  
Human Beings ◽  
Peritoneal Adhesion ◽  
Adhesion Barrier ◽  
Anastomosis Leakage ◽  
Barrier Film

BACKGROUND: Postoperative adhesion is still a consequence of intra-abdominal surgeries, which results in bowel obstruction and abdominopelvic pain. Bowel anastomosis as a common abdominal surgery has the incidence of leakage in up to 30% of patients that increase morbidity and mortality. Due to similar pathways of adhesion formation and wound healing, it is important to find a way to reduce adhesions and anastomosis leakage. AIM: This study was designed to compare antiadhesive as well as anastomosis healing improvement effect of honey and polylactide anti-adhesive barrier film. METHODS: Forty-five rabbits divided into three groups of honey, adhesion barrier film, and control group in an animal study. Under a similar condition, rabbits underwent resection and anastomosis of cecum under general anaesthesia. In the first group, honey was used at the anastomosis site, in the second one polylactide adhesion barrier film utilised, and the third one was the control group. Adhesion, as well as anastomosis leakage, was assessed after 21 days. Data were analysed using the Statistical Package for Social Scientists (SPSS) for Windows version 25. RESULTS: Three groups of 15 rabbits were studied. The results showed that mean peritoneal adhesion score (PAS) was lower in the honey group (1.67) in comparison to the adhesion barrier film group (3.40) and the control group (6.33). CONCLUSION: Bio-absorbable polylactide barrier has an anti-adhesion effect but is not suitable for intestinal anastomosis in rabbits. Further studies needed to evaluate these effects on human beings.

Prevention of Intra-Abdominal Adhesions Using the Combination of Mediclore® and a Statin

2021 ◽  
pp. 1-9
Author(s):  
Jesung Park ◽  
Hyun Kang ◽  
Yoo Shin Choi ◽  
Suk-Won Suh ◽  
Soon Auck Hong ◽  
...  
Keyword(s):  
Chronic Inflammation ◽  
Acute Inflammation ◽  
Adhesion Formation ◽  
Male Rats ◽  
Control Group ◽  
Fibrosis Score ◽  
Abdominal Adhesions ◽  
Group Assignment ◽  
Control Group Group ◽  
Inflammation Score

<b><i>Purpose:</i></b> This study investigated the antiadhesive effects of Mediclore®, rosuvastatin, and a combination of Mediclore and rosuvastatin in a rat adhesion model. <b><i>Methods:</i></b> The adhesion models (a total of 58 adult male rats) were divided into 4 groups. The control group (group C) received no special materials except for a saline. The experimental groups were treated with 5 mL of Mediclore (group M), rosuvastatin (group R), or rosuvastatin and Mediclore (group RM), and these materials were intraperitoneally placed under the incision. At postoperative day 14, the rats underwent re-laparotomy and adhesiolysis. Three investigators blinded to group assignment scored the extent of adhesion formation, the numbers of remote adhesions, and the extent of acute/chronic inflammation, fibrosis, edema, and congestion on resected specimens via histologic examination. <b><i>Results:</i></b> The macroscopic adhesion score in group RM (7.27 ± 3.51) was significantly lower than those in groups C (13.36 ± 2.24) and R (11.71 ± 1.98); group M (9.13 ± 4.09) had a significantly lower adhesion score than group C. The number of remote adhesions was significantly lower in groups R and RM than in group C. The acute inflammation score, chronic inflammation score, and fibrosis score in group RM; the acute inflammation score in group R; and the fibrosis score in group M were significantly lower than those in group C. <b><i>Conclusion:</i></b> The intraperitoneal application of Mediclore and a combination of Mediclore and rosuvastatin effectively reduced postoperative adhesions.

scholarly journals Informativeness of phenotypic features of connective tissue dysplasia in children with peritoneal adhesions

2016 ◽  
Vol 23 (3) ◽  
Author(s):  
M. G. Melnichenko ◽  
A. A. Kvashnina
Keyword(s):  
Connective Tissue ◽  
Adhesion Formation ◽  
Integrated Approach ◽  
Postoperative Adhesion ◽  
Abdominal Adhesions ◽  
Additional Time ◽  
Peritoneal Adhesions ◽  
Phenotypic Characters ◽  
Clinical Variants ◽  
Connective Tissue Dysplasia

Connective tissue dysplasia can be realized with a large number of clinical variants, and accordingly, excessive postoperative adhesion formation can be considered as a manifestation of dysplastic-dependent processes. In the predominant number of surveyed children (93.8%) CTD has developed on the background of existing connective tissue dysplasia syndrome, i.e. the presence of external signs of this condition may serve as a predictor of postoperative complications of adhesions in children.Direct correlation between the number of CTD phenotypic characters and the prevalence of intra-abdominal adhesions was determined. In our view, it allows to detect children at risk of peritoneal adhesions on the basis of external features that can be identified during general examination and do not require additional time or equipment. Accordingly, the surgical treatment of children with signs of CTD syndrome requires an integrated approach and the application of measures to prevent excessive adhesion formation, including intraoperative use of anti-adhesive gels.

Decrease in VEGF-Induced Pericardial Adhesion Formation Using Bevacizumab After Surgery

2018 ◽  
Vol 26 (1) ◽  
pp. 21-26 ◽  
Author(s):  
Hosseinali Jelveh Moghaddam ◽  
Marjan Aghajani ◽  
Ehsan Raeis-Abdollahi ◽  
Mahdieh Faghihi ◽  
Ali Dabbagh ◽  
...  
Keyword(s):  
Inflammatory Responses ◽  
Adhesion Formation ◽  
High Sensitivity ◽  
Postoperative Adhesion ◽  
Control Group ◽  
C Reactive Protein ◽  
Protein Levels ◽  
Reactive Protein ◽  
Cd 31 ◽  
Pericardial Adhesions

Objectives. Some degrees of postoperative cardiac adhesions occur in response to the first cardiac surgery in patients that may limit surgeons for subsequent operations and increase the risk of heart injury. In this article, we established a model of postoperative pericardial adhesions, and because vascular endothelial growth factor (VEGF) seems to initiate adhesion formation through inflammatory responses, we used an anti-VEGF antibody, that is, bevacizumab, to examine its effects on postoperative adhesion formation. Methods. Twenty Wistar rats were divided in 2 groups: control and bevacizumab. After chest opening, pericardial sac was opened and the heart was fully exposed. In the bevacizumab group, bevacizumab (2.5 mg/kg) was applied locally on the heart and then the chest was closed. The control group received saline solution as placebo. After 42 days, high-sensitivity C-reactive protein in peripheral blood was measured, and re-sternotomy was performed to measure severity of pericardial adhesions. Then, the hearts were collected from all rats to evaluate percentage of CD-31-positive cells (as a marker of angiogenesis) using immunohistochemical staining. Results. When the bevacizumab group was compared with the control group, we found that the mean score of adhesion (0.89 ± 0.38 vs 2.56 ± 0.41) and CD-31 expression (27.45 ± 3.75% vs 56.26 ± 1.98%) was decreased significantly after bevacizumab administration. However, we did not find any difference in high-sensitivity C-reactive protein levels of control and bevacizumab animals. Conclusion. In the current study, bevacizumab administration could effectively reduce adhesion formation after first sternotomy by preventing VEGF-induced angiogenesis through CD-31 downregulation.

scholarly journals Prevention of intra-abdominal adhesions by a hyaluronic acid gel; an experimental study in rats

2020 ◽  
pp. 088532822095418
Author(s):  
Sebastiaan van Steensel ◽  
Hong Liu ◽  
Timon FG Vercoulen ◽  
M’hamed Hadfoune ◽  
Stephanie O Breukink ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Macrophage Polarization ◽  
Adhesion Formation ◽  
Rank Test ◽  
Control Group ◽  
P Value ◽  
Abdominal Adhesions ◽  
Male Wistar Rats ◽  
Acid Gel ◽  
Hyaluronic Acid Gel

Background In 80% to 90% of the patients intra-abdominal adhesions occur after abdominal surgery, which can cause small-bowel obstruction, chronic abdominal pain, female infertility and difficulty during reoperation. A novel crosslinked hyaluronic acid gel is evaluated regarding its anti-adhesive capacities in an ischemic button model in rats. Method 51 adult, male Wistar rats from a registered breeder, received eight ischemic buttons each and were treated with hyaluronic acid gel (HA, HyaRegen©), hyaluronic acid carboxymethylcellulose (HA-CMC, Seprafilm©) or no anti-adhesive barrier. After 14 days, the animals were sacrificed and adhesions were scored macroscopically. The number of buttons and organs involved in adhesions were recorded. Per animal, one button with adhesions and one without adhesions was explanted for qPCR analysis. Mann-Whitney U, Fisher’s exact and Wilcoxon signed rank test were used for data analysis. A p-value of 0.05 was considered significant. Results Macroscopic evaluation of adhesion formation did not differ between the groups. The number of organs involved in adhesions in the HA gel group was significantly lower compared to HA-CMC (p = .041) and the control group (p = .012). A significantly, 1.36-fold higher clec10a (p = 0.25), 1.80-fold higher cd163 (p = 0.003) and 5.14-fold higher mmp1 expression (p = 0.028) was found in ischemic buttons with adhesions compared to buttons without adhesions. Conclusion HA gel application reduces the number of organs involved in adhesions in an ischemic button model, but no overall reduction in adhesion formation was encountered. Macrophage subtype 2 polarization and high mmp1 expression are associated with adhesion formation. Further investigation is needed in the exact pathophysiologic process of adhesion formation and the role of macrophage polarization.

scholarly journals A Randomized Controlled Trial of Thermo-Sensitive Sol–Gel Anti-Adhesion Agent after Gynecologic Surgery

2020 ◽  
Vol 9 (7) ◽  
pp. 2261
Author(s):  
Young Im Kim ◽  
Maria Lee ◽  
Se Ik Kim ◽  
Aeran Seol ◽  
Eun Ji Lee ◽  
...  
Keyword(s):  
Controlled Trial ◽  
Sol Gel ◽  
Adhesion Formation ◽  
Gynecologic Surgery ◽  
Control Group ◽  
Abdominal Adhesions ◽  
Randomized Controlled ◽  
Slide Test ◽  
And Control ◽  
Experimental Group

Postoperative abdominal adhesions can lead to several adverse consequences such as pelvic pain, bowel obstruction, and infertility. We aimed to explore the anti-adhesion efficacy and safety of a thermo-sensitive sol–gel agent in patients who receive abdominopelvic surgery for benign gynecologic disease. This study was a randomized, controlled, single-blind clinical trial of women undergoing benign gynecologic surgery between January 2017 and December 2017. The patients were randomly assigned to three groups with a 1:1:1 ratio: experimental group (received the thermo-sensitive sol–gel agent), control group (untreated), and comparator group (received 4% icodextrin). Patients were followed for 4 weeks postoperatively, and efficacy was evaluated by performing the visceral slide test to identify adhesion formation. In total, 183 patients were enrolled in the study, and 178 (97.3%) completed the trial. The incidence rate of abdominal adhesion formation was significantly lower in the experimental group than in the control group (7.9% vs. 21.1%, p = 0.040); however, it was similar between the experimental and comparator groups (7.9% vs. 13.8%. p = 0.299). At 4 weeks, no differences in adhesion-related symptoms were observed between the experimental and control groups. Adverse events were mostly mild and did not differ significantly among the three groups (p = 0.375). In conclusion, use of a thermo-sensitive sol–gel agent was safe and effective to prevent abdominal adhesions after benign gynecologic surgeries.

Efficacy of Tenoxicam on Intra-Abdominal Adhesion Prevention in a Rat Model

1996 ◽  
Vol 24 (4) ◽  
pp. 352-357 ◽  
Author(s):  
T Yilmazlar ◽  
E Kaya ◽  
E Gürpinar ◽  
H Emiroğlu
Keyword(s):  
Rat Model ◽  
Abdominal Cavity ◽  
Adhesion Formation ◽  
Test Material ◽  
Adhesion Prevention ◽  
Control Group ◽  
Albino Rats ◽  
Abdominal Adhesion ◽  
Wistar Albino Rats ◽  
The Right

The aim of this study was to investigate the effect of tenoxicam as a non-steroidal anti-inflammatory drug (NSAID) on intra-abdominal adhesion prevention in a rat model. Altogether 50 Wistar-Albino rats weighing 220 – 280 g were assigned to five groups, each of which was made up of 10 rats. All the rats were anaesthetized and prepared for sterile surgery. After a mid-line laparotomy was performed, a 1 cm area of the caecum was rubbed with gauze until subserosal haemorrhage developed, and then a 5 mm-diameter part of the peritoneum on the right side of the abdominal wall was removed. Prior to complete closure, 3 ml of the test material was placed into the abdominal cavity. On the eighth day the rats were killed and the adhesion score was determined. The groups and their mean adhesion scores were as follows: control group (normal saline), 2.5; group of dilution buffer, 1.8; tenoxicam (0.125 mg/kg), 1.3; tenoxicam (0.25 mg/kg), 1.3; and tenoxicam (0.5 mg/kg), 0.9. The differences between the adhesion scores among all the groups ( P < 0.05, Kruskal-Wallis test), and those between the tenoxicam groups and control group ( P < 0.05, Mann-Whitney U-test), were significant. Thus a single instillation of tenoxicam into the peritoneal cavity at the time of surgery reduced adhesion formation effectively in this model, irrespective of dosage.

scholarly journals The Anti-Adhesive Effect of Granulocyte-Colony Stimulating Factor on an Abdominal Adhesion Model in Sprague-Dawley Rats

2020 ◽  
Author(s):  
In-Hwa Park ◽  
Yi-Sun Song ◽  
Hyun-Woo Joo ◽  
Guang-Yin Shen ◽  
Jin-Hee Seong ◽  
...  
Keyword(s):  
Adhesion Formation ◽  
The Other ◽  
Mrna Levels ◽  
Granulocyte Colony Stimulating Factor ◽  
Colony Stimulating Factor ◽  
Abdominal Adhesions ◽  
Abdominal Adhesion ◽  
Severity Scores ◽  
Adhesive Effect ◽  
Stimulating Factor

Abstract Background: Intra-abdominal adhesions are among the most common complications following abdominal surgery. In this study, using an abdominal adhesion rat model, we investigated the anti-adhesive effect of granulocyte-colony stimulating factor (G-CSF) on intra-abdominal adhesions. Methods: All rats were laparotomized with 3 ischemic peritoneal buttons developed to cause adhesions. The experimental rats were divided randomly into 3 groups (n=8/group): control, pluronic gel, and G-CSF groups. Fourteen days after surgery, all rats were sacrificed, and intra-abdominal adhesions were assessed. The percentage of adhesions, adhesion severity scale and density of adhesion formation were evaluated. Real-time PCR was conducted to assess the cytokine mRNA levels of substance P (SP), neurokinin 1 receptor (NK-1R), transforming growth factor-β1 (TGF-β1), and intercellular adhesion molecule-1 (ICAM-1).Results: The severity scores of intra-abdominal adhesions of, and the degree of adhesion formation in, rats treated with G-CSF were significantly lower than those in case of rats from other groups. Additionally, in the G-CSF group, the number of ischemic buttons with developed adhesions was significantly lower than that in the other groups. In adhesion samples of the G-CSF group, the expression level of SP was significantly lower than that in the other groups.Conclusions: Our study demonstrated that G-CSF treatment decreases the formation of intra-abdominal adhesion after surgery by reducing inflammatory reactions in adhesion tissues.

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