scholarly journals Rapid Biological Synthesis of Silver Nanoparticles from Ocimum sanctum and Their Characterization

2017 ◽  
Vol 2017 ◽  
pp. 1-6 ◽  
Author(s):  
M. Z. H. Khan ◽  
F. K. Tarek ◽  
M. Nuzat ◽  
M. A. Momin ◽  
M. R. Hasan
Keyword(s):  
Silver Nanoparticles ◽  
Exothermic Reaction ◽  
Synthesis Process ◽  
Biological Synthesis ◽  
Ocimum Sanctum ◽  
Metallic Silver ◽  
Gravimetric Analysis ◽  
Face Centered Cubic ◽  
Average Grain Size ◽  
Amine Groups

With development of nanotechnology, the biological synthesis process deals with the synthesis, characterization, and manipulation of materials and further development at nanoscale which is the most cost-effective and eco-friendly and rapid synthesis process as compared to physical and chemical process. In this research silver nanoparticles (AgNPs) were synthesized from silver nitrate (AgNO3) aqueous solution through eco-friendly plant leaf broth of Ocimum sanctum as reactant as well as capping agent and stabilizer. The formation of AgNPs was monitored by ultraviolet-visible spectrometer (UV-vis) and Fourier transform infrared (FTIR) spectroscopy. X-ray diffraction (XRD) and scanning electronic microscopy (SEM) have been used to characterize the morphology of prepared AgNPs. The peaks in XRD pattern are in good agreement with that of face-centered-cubic (FCC) form of metallic silver. Thermal gravimetric analysis/differential thermal analysis (TGA/DTA) results confirmed the weight loss and the exothermic reaction due to desorption of chemisorbed water. The average grain size of silver nanoparticles is found to be 29 nm. The FTIR results indicated that the leaf broths containing the carboxyl, hydroxyl, and amine groups are mainly involved in fabrication of silver AgNPs and proteins, which have amine groups responsible for stabilizing AgNPs in the solution.

scholarly journals Synthesis and Characterization of Silver Nanoparticles from Ashyranthus aspera Extract for Antimicrobial Activity Studies

2020 ◽  
Vol 24 (7) ◽  
pp. 1161-1167
Author(s):  
V. Swetha ◽  
S Lavanya ◽  
G. Sabeena ◽  
E. Pushpalaksmi ◽  
Samraj J. Jenson ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Antimicrobial Agents ◽  
Exothermic Reaction ◽  
Visual Observation ◽  
Metallic Silver ◽  
Face Centered Cubic ◽  
Leaf Extracts ◽  
Synthesis Of Nanoparticles ◽  
Potential Applications

Development of biologically inspired experimental processes for the synthesis of nanoparticles is evolving into an important branch of  nanotechnology. Plant-mediated synthesis of nanomaterials has been increasingly gaining popularity due to its eco-friendly nature and cost-effectiveness. In the present study, we were synthesized silver (Ag) nanoparticles using aqueous extracts of fresh leaves of Ashyranthus aspera medicinal plants as bio-reducing agents. UV-Vis spectrometer used to monitor the reduction of Ag ions and the formation of AgNPs in the medium. UV-Vis spectra and visual observation showed that the color of the fresh leaf extracts of Ashyranthus aspera turned into grayish-brown respectively, after treatment with Ag precursors. XRD and SEM have been used to investigate the morphology of prepared AgNPs. The peaks in the XRD pattern are associated with that of the Face-Centered-Cubic (FCC) form of metallic silver. TGA/DTA results associated with weight loss and exothermic reaction due to the desorption of chemisorbed water. FTIR was performed to identify the functional groups which form a layer covering AgNPs and stabilize the AgNPs in the medium. Moreover, silver nanoparticles using aqueous leaf extracts of Ashyranthus aspera were separately tested for their antibacterial activity against Gram-positive bacteria (Staphylococcus aureus) and Gram-negative bacteria (Enterobacter). The results showed that the bacterial growth was inhibited by the extracts containing AgNPs Nanoparticles. The biosynthesized nanoparticle was prepared from Ashyranthus aspera leaf extracts exhibits potential applications as broad-spectrum antimicrobial agents Keywords: Ashyranthus aspera, Silver Nanoparticles, Plant extracts, Bacteria, Antibacterial activity.

Fabrication of Silver Nanoparticles and its Films and their Characterization of Structure and Electrical Conductivity

2014 ◽  
Vol 896 ◽  
pp. 341-346 ◽  
Author(s):  
Markus Diantoro ◽  
Alif Fitria Chasanah ◽  
Nasikhudin ◽  
Nandang Mufti ◽  
Abdulloh Fuad
Keyword(s):  
Electrical Conductivity ◽  
Silver Nanoparticles ◽  
Nano Particles ◽  
Metallic Silver ◽  
Cubic Phase ◽  
Face Centered Cubic ◽  
X Ray ◽  
Average Grain Size ◽  
Face Centered

Based on our previous work of MSA influence on silver nano particles formation we have performed a series of research related to the preparation and characterization of silver nanoparticles which synthesized through coprecipitation process. The silver nano particles was transformed into silver nanoparticles films by spin coating deposition method. The silver nanoparticles were prepared from silver acetate as precursor, reductant agent phenylhydrazine, and dodecylamine as stabilizer. X-Ray Fluorescence (XRF), X-Ray Diffraction (XRD), Scanning Electron Microscopy (SEM), Energy Dispersive X-Ray Analysis (EDAX), and Electric Four Point Probe have been used to characterize the silver nanoparticles as well as the films. The morphology and the crystal structure have been determined by means of XRD and SEM. The average grain size of silver nanoparticles is found to be 22 nm. The peaks in XRD pattern are in good agreement with that of face-centered-cubic phase of metallic silver. The electrical conductivity films were confirmed in the range of conducting material. The highest conductivity was reached at 3,88 x 109 S/cm associated to 1600 rpm of spin coater rotation.

scholarly journals Synthesis of Silver Nanoparticles UsingTriticum aestivumand Its Effect on Peroxide Catalytic Activity and Toxicology

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Shobha Waghmode ◽  
Pooja Chavan ◽  
Vidya Kalyankar ◽  
Sharada Dagade
Keyword(s):  
Silver Nanoparticles ◽  
Catalytic Activity ◽  
Large Scale ◽  
Visible Spectrum ◽  
Biological Synthesis ◽  
Metallic Silver ◽  
Scale Production ◽  
Face Centered Cubic ◽  
Cubic Form ◽  
Uv Visible

The synthesis of stable silver nanoparticles using bioreduction method was investigated. Biological synthesis of silver nanoparticles usingTriticum aestivum(khapali ghahu) extract was investigated. The effect of a specific variety of plants and how it affects the growth of silver nanoparticles was investigated in our work and it was polydispersed. UV-visible spectroscopy was used to monitor the formation of silver nanoparticles within 15 minutes. The peaks in XRD pattern are in good agreement with those of face-centered-cubic form of metallic silver. Further the IR and TEM shows confirmation of nanocrystalline nature of silver nanoparticles. These nanoparticles dislodged by ultrasonication showed an absorption peak at 430 nm in UV-visible spectrum corresponding to the Plasmon resonance of silver nanoparticles. UV-visible titration experiments showed evidence that silver nanoparticles facilitate hydrogen peroxide reduction showing excellent catalytic activity at 200 μL. In this preliminary toxicology study, Earthworm toxicology we checked and is stable up to 1500 ppm concentration. The use of plant extract for silver nanoparticles synthesis offers the benefits of eco-friendliness and amenability for large-scale production.

scholarly journals Green Synthesis of Silver Nanoparticles by Extracellular Extracts from Aspergillus japonicus PJ01

Molecules ◽  
2021 ◽  
Vol 26 (15) ◽  
pp. 4479
Author(s):  
Pei-Jun Li ◽  
Jiang-Juan Pan ◽  
Li-Jun Tao ◽  
Xia Li ◽  
Dong-Lin Su ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Properties ◽  
Antimicrobial Properties ◽  
Antibacterial Activities ◽  
Synthesis Process ◽  
Biological Synthesis ◽  
X Ray Diffraction ◽  
Aspergillus Japonicus ◽  
Transmission Electron ◽  
Naoh Solution

The present study focuses on the biological synthesis, characterization, and antibacterial activities of silver nanoparticles (AgNPs) using extracellular extracts of Aspergillus japonicus PJ01.The optimal conditions of the synthesis process were: 10 mL of extracellular extracts, 1 mL of AgNO3 (0.8 mol/L), 4 mL of NaOH solution (1.5 mol/L), 30 °C, and a reaction time of 1 min. The characterizations of AgNPs were tested by UV-visible spectrophotometry, zeta potential, scanning electron microscope (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), and thermogravimetric (TG) analyses. Fourier transform infrared spectroscopy (FTIR) analysis showed that Ag+ was reduced by the extracellular extracts, which consisted chiefly of soluble proteins and reducing sugars. In this work, AgNO3 concentration played an important role in the physicochemical properties and antibacterial properties of AgNPs. Under the AgNO3 concentration of 0.2 and 0.8 mol/L, the diameters of AgNPs were 3.8 ± 1.1 and 9.1 ± 2.9 nm, respectively. In addition, smaller-sized AgNPs showed higher antimicrobial properties, and the minimum inhibitory concentration (MIC) values against both E. coli and S. aureus were 0.32 mg/mL.

scholarly journals Hypericum perforatum L.-Mediated Green Synthesis of Silver Nanoparticles Exhibiting Antioxidant and Anticancer Activities

Nanomaterials ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 487 ◽  
Author(s):  
Abdalrahim Alahmad ◽  
Armin Feldhoff ◽  
Nadja C. Bigall ◽  
Pascal Rusch ◽  
Thomas Scheper ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Hypericum Perforatum ◽  
Nitrate Solution ◽  
A549 Cells ◽  
Metallic Silver ◽  
Synthesis Methods ◽  
Face Centered Cubic ◽  
Sem Images ◽  
Hypericum Perforatum L

This contribution focuses on the green synthesis of silver nanoparticles (AgNPs) with a size < 100 nm for potential medical applications by using silver nitrate solution and Hypericum Perforatum L. (St John’s wort) aqueous extracts. Various synthesis methods were used and compared with regard to their yield and quality of obtained AgNPs. Monodisperse spherical nanoparticles were generated with a size of approximately 20 to 50 nm as elucidated by different techniques (SEM, TEM). XRD measurements showed that metallic silver was formed and the particles possess a face-centered cubic structure (fcc). SEM images and FTIR spectra revealed that the AgNPs are covered by a protective surface layer composed of organic components originating from the plant extract. Ultraviolet-visible spectroscopy, dynamic light scattering, and zeta potential were also measured for biologically synthesized AgNPs. A potential mechanism of reducing silver ions to silver metal and protecting it in the nanoscale form has been proposed based on the obtained results. Moreover, the AgNPs prepared in the present study have been shown to exhibit a high antioxidant activity for 2, 2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) radical cation, and super oxide anion radical and 2,2-diphenyl-1-picrylhydrazyl. Synthesized AgNPs showed high cytotoxicity by inhibiting cell viability for Hela, Hep G2, and A549 cells.

scholarly journals Marine plant mediated green synthesis of silver nanoparticles using mangrove Rhizophora stylosa: Effect of variable process and their antibacterial activity

F1000Research ◽  
2021 ◽  
Vol 10 ◽  
pp. 768
Author(s):  
Nancy Willian ◽  
Syukri Syukri ◽  
Zulhadjri Zulhadjri ◽  
Syukri Arief
Keyword(s):  
Staphylococcus Aureus ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Leaf Extract ◽  
Nitrate Solution ◽  
Narrow Particle Size Distribution ◽  
Metallic Silver ◽  
Face Centered Cubic ◽  
Rhizophora Stylosa ◽  
Vis Spectroscopy

Background: Most natural plants used in the synthesis of silver nanoparticles are limited to marine plants. To carry out applications, colloidal silver nanoparticles (AgNps) should have appropriate properties such as homogeneous shapes, small and narrow particle size distribution, and long time stability. This study aims to determine the effects of a variable process of AgNps mediated mangrove Rhizophora stylosa (RS) leaf extract, and antibacterial activity.  Methods: Synthesis of AgNps was carried out by stirring silver nitrate solution with aqueous extract. The characterization of AgNps was carried out using UV-Vis spectrophotometry, X-ray diffraction (XRD), Dynamic Light Scattering (DLS) zetasizer and Transmission Electron Microscopy (TEM). Evaluation of antibacterial activity was carried out on Escherichia coli and Staphylococcus aureus. Reaction conditions such as the concentration of metal ions (0.001 M, 0.005 M, and 0.01 M), extracts (1%, 3%, and 5% v/v), and the reaction time on the size and stability of nanoparticles were also explored. Results: The UV-Vis spectroscopy showed an absorption of colloidal AgNps in a wavelength range of 403–443 nm.  TEM analysis showed that as-synthesized AgNps were spherical in shape with a size range of 5–87 nm. The use of 0.001 M and 0.005 M of Ag+ resulted in a smaller diameter than the synthesized AgNps, using 0.01 M Ag+, in the same extract concentration. The range of zeta potential was -24.9 mV to -27.7 mV. The as-synthesized AgNps were stable for more than one month. The XRD analysis showed four peaks, which were attributed to the face centered cubic crystal structure of metallic silver. The results of the silver nanoparticles synthesis showed good activity on Escherichia coli and Staphylococcus aureus, with an inhibition zone between 4.1–7.2 mm. Conclusions: The AgNps synthesized with RS leaf extract, which is a reducing agent, showed good potential as an antibacterial component.

scholarly journals EXTRACELLULAR BIOSYNTHESIS OF SILVER NANOPARTICLES USING FUNGUS PENICILLIUM SPECIES

2018 ◽  
Vol 6 (1) ◽  
pp. 277-283
Author(s):  
Tejal Barkhade
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Color Change ◽  
Good Alternative ◽  
Synthesis Process ◽  
Biological Synthesis ◽  
Penicillium Species ◽  
Synthesis Of Nanoparticles ◽  
Extracellular Biosynthesis ◽  
Agno3 Solution

Synthesis of nanoparticles by biological process is good alternative as compare to physical and chemical processes because it’s very easy, non-toxic, eco-friendly, and cheap. Biological synthesis combines biological principles (i.e., reduction/oxidation) by microbial enzymes. The advantages of fungal-mediated biosynthesis of nanoparticles have turned the attention of scientists to the kingdom of fungi. The present research study shows the biological synthesis of silver nanoparticles (AgNPs) by using filamentous fungus Penicillium sp. The biomass was harvested after 72 hours of growth by sieving through a plastic sieve and from this biomass cell filtrate was obtained for the synthesis process. The synthesis process was quite fast; when silver ion was interact with cell filtrate, within few minutes. After 24 hour of incubation period in dark condition, colorless cell filtrate solution with AgNO3 solution was turns in to brown color solution; that means synthesis process of silver nanoparticles was confirmed due to color change reaction. The characterization of synthesized Silver nanoparticles was done by Transmission Electron Microscopy (TEM) and Scanning Electron Microscopy (SEM), UV-Visible spectroscopy.

scholarly journals Biomimetic Synthesis of Silver Nanoparticles Using Endosymbiotic Bacterium InhabitingEuphorbia hirtaL. and Their Bactericidal Potential

Scientifica ◽  
2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Baker Syed ◽  
Hoovinakola Chinnappa Yashavantha Rao ◽  
Mysore Nagalingaswamy Nagendra-Prasad ◽  
Ashwini Prasad ◽  
Ballagere Puttaraju Harini ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Pathogenic Bacteria ◽  
Morphological Characteristics ◽  
Endophytic Bacterium ◽  
Biomimetic Synthesis ◽  
Metallic Silver ◽  
Face Centered Cubic ◽  
Euphorbia Hirta ◽  
Synthesis Of Nanoparticles ◽  
Face Centered

The present investigation aims to evaluate biomimetic synthesis of silver nanoparticles using endophytic bacterium EH 419 inhabitingEuphorbia hirtaL. The synthesized nanoparticles were initially confirmed with change in color from the reaction mixture to brown indicating the synthesis of nanoparticles. Further confirmation was achieved with the characteristic absorption peak at 440 nm using UV-Visible spectroscopy. The synthesized silver nanoparticles were subjected to biophysical characterization using hyphenated techniques. The possible role of biomolecules in mediating the synthesis was depicted with FTIR analysis. Further crystalline nature of synthesized nanoparticles was confirmed using X-ray diffraction (XRD) with prominent diffraction peaks at 2θwhich can be indexed to the (111), (200), (220), and (311) reflections of face centered cubic structure (fcc) of metallic silver. Transmission electron microscopy (TEM) revealed morphological characteristics of synthesized silver nanoparticles to be polydisperse in nature with size ranging from 10 to 60 nm and different morphological characteristics such as spherical, oval, hexagonal, and cubic shapes. Further silver nanoparticles exhibited bactericidal activity against panel of significant pathogenic bacteria among whichPseudomonas aeruginosawas most sensitive compared to other pathogens. To the best of our knowledge, present study forms first report of bacterial endophyte inhabitingEuphorbia hirtaL. in mediating synthesizing silver nanoparticles.

scholarly journals Chromatographic analysis of phytochemicals in the peel of Musa Paradisiaca to synthesize silver nanoparticles

2021 ◽  
Author(s):  
María José Buendia-Otero ◽  
Deisy Julieth Jiménez-Corzo ◽  
Zulia Isabel Caamaño De Ávila ◽  
Juan Bernardo Restrepo
Keyword(s):  
Silver Nanoparticles ◽  
Metallic Nanoparticles ◽  
Average Particle Size ◽  
Research Work ◽  
Reducing Agent ◽  
Metallic Silver ◽  
Average Particle ◽  
Face Centered Cubic ◽  
Musa Paradisiaca ◽  
Xrd Techniques

This research work used Musa Paradisiaca (banana) peels as a natural solvent, assorted with the precursor AgNO3 (10 mM) to perform the green synthesis of silver nanoparticles. The phytochemical components present in the Musa Paradisiaca peel extracts were determined by gas chromatography coupled to a mass spectrometer (GC-MS), and it was possible to identify the compounds: 1.2 Ethanediol (60.0261 %) and 2.3 Butanediol (11.2 %); these -diols represent a highly reducing agent for metals, since they act as a solvent for the metal precursor behaving as a reducing agent, and facilitating the formation of nanoparticles. Likewise, the synthesized silver nanoparticles were subjected to a washing and drying treatment to be subsequently characterized by means of UV-Vis and XRD techniques, resulting in a wavelength of 411 nm, which is characteristic of these metallic nanoparticles, and achieving the identification of the face-centered cubic structure (fcc) of the metallic silver, with an average particle size of 21.8 nm according to the Debye-Scherrer equation.

scholarly journals Antibacterial Activity and Characteristics of Silver Nanoparticles Biosynthesized From Carduus Crispus

2021 ◽  
Author(s):  
Enerelt Urnukhsaikhan ◽  
Bum-Erdene Bold ◽  
Aminaa Gunbileg ◽  
Nominchimeg Sukhbaatar ◽  
Tsogbadrakh Mishig-Ochir
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Functional Groups ◽  
Micrococcus Luteus ◽  
Blue Shift ◽  
High Yield ◽  
Face Centered Cubic ◽  
Average Grain Size ◽  
Medical Plant ◽  
Face Centered

Abstract Recently, synthesizing metal nanoparticles using plants has been extensively studied and recognized as a non-toxic and efficient way for biomedical field. The aim of this study is to investigate the role of different parts of Carduus crispus medical plant on synthesizing silver nanoparticles and their characteristics. Our study showed that silver nanoparticles (AgNP) synthesized via whole plant extract exhibited a blue shift in absorption spectra with increased optical density, which correlates to a high yield and smaller size. Also, the results of zeta potential, XRD, PCCS analysis showed the surface charge of -54.29 ±4.96 mV (AgNP-S), -42.64 ±3.762 mV (AgNP-F), -46.02 ±4.17 mV (AgNP-W), the crystallite size of 36 nm (AgNP-S), 13 nm (AgNP-F), 14 nm (AgNP-W) with face-centered cubic structure and average grain size of approximately 100 nm. Another important characteristic, such as elemental composition and constituent capping agent has been determined by EDX and FTIR. The silver nanoparticles were composed of ~80% Ag, ~15% K, and ~7.5% Ca (or ~2.8% P) elements. Moreover, the results of the FTIR measurement suggested that AgNP-F and AgNP-S contained distinct functional groups, on the other hand, AgNP-W contained all of the functional groups present in AgNP-F and AgNP-S. The silver nanoparticles showed antibacterial activity on both gram-negative bacterium Escherichia coli (5.5 ± 0.2 mm to 6.5 ± 0.3 mm) and gram-positive bacterium Micrococcus luteus (7 ± 0.4 mm to 7.7 ± 0.5 mm). Our study is meaningful as a first observation indicating the possibility of using special plant organs to control the characteristics of nanoparticles.

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