scholarly journals Exploring theUrtica dioicaLeaves Hemostatic and Wound-Healing Potential

2017 ◽  
Vol 2017 ◽  
pp. 1-10 ◽  
Author(s):  
Karama Zouari Bouassida ◽  
Sana Bardaa ◽  
Meriem Khimiri ◽  
Tarek Rebaii ◽  
Slim Tounsi ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Wound Healing ◽  
Unsaturated Fatty Acids ◽  
Antioxidant Activities ◽  
High Concentration ◽  
Leaf Extracts ◽  
Traditional Use ◽  
Wound Healing Effect ◽  
Epidermal Regeneration

The present paper investigated the efficiency ofUrtica dioica (U. dioica)on hemostatic and wound healing activities.U. dioicaleaf extracts were evaluated for their antibacterial and antioxidant effects as well as their flavonoid and polyphenol content. The hydroethanolic extract (EtOH-H2OE), showing the most potent antibacterial and antioxidant activitiesin vitro, thanks to its flavonoid and polyphenol richness, was selected for hemostatic and wound healing evaluation. Twenty-four rats completing full-thickness wounds were split into four groups. The wounds were topically treated with saline solution, glycerol, “CICAFLORA,” andU. dioicaEtOH-H2OE (50 µL/mm2) until day 11. The wound healing effect was assessed by macroscopic, histological, and biochemical parameters. Rats treated with EtOH-H2OE showed fast wound closure (92.39%) compared to the control animals (60.91%) on the 11th day of wounding (P<0.01). Histopathological and biochemical explorations showed full epidermal regeneration and an improvement of the hydroxyproline content in theU. dioicaEtOH-H2OE treated rats. Analysis of fatty acids and sterols by GC-MS showed the presence of unsaturated fatty acids and a high concentration of lupeol known for their involvement in reepithelialization. These results prove the efficiency ofU. dioicaEtOH-H2OE in wound healing and supported its traditional use.

scholarly journals In Vitro Antioxidant, Membrane Stabilizing and Antimicrobial Properties of Murayya paniculata Wild Plant of Amarkantak (M.P)

2021 ◽  
Author(s):  
Shruti Sonter ◽  
Shringika Mishra ◽  
Manish Dwivedi ◽  
Prashant Singh
Keyword(s):  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antimicrobial Activities ◽  
Hexane Extract ◽  
Wild Plant ◽  
Hypotonic Solution ◽  
High Concentration ◽  
Leaf Extracts ◽  
Traditional Use

Abstract The excessive usage of antibiotics in humans and veterinary medicine has lead to the emergence of antibiotic resistance and requirement of novel antibiotics. There has been increased interest towards plants as source of drugs because of their pharmacological potency and long traditional usage. The aim of the current study was to evaluate bioactive components, antioxidant and anti-inflammatory activity from the leaf extracts of Murayya paniculata, a traditionally used Indian medicinal plant. Evaluations were made for phytochemicals, antioxidant, membrane stabilizing and antimicrobial activities. The methanol extract displayed highest flavonoid and phenolic content while the acetone extract demonstrated considerable ABTS inhibitory activity (IC50value:555.18 ± 1.68 µg/mL) and hexane extract exhibited highest H2O2 radical scavenging activity (IC50value: 509.84 ± 3.03 µg/mL). The aqueous extract displayed 19.4 ± 0.66% RBC hemolysis and 80.5 ± 0.66% protection caused by hypotonic solution at high concentration of extract. The fractions of hexane extract revealed a higher zone of inhibition than crude extract. The major components found in the fractions were cyclohexane (40.11%) and 3-(6-Methoxy-3-methyl-2-benzofuranyl) Cyclohexanone (13.68%) analyzed by GC-MS technique. The current results validate the traditional use of the plant and warrant its potential in drug development programs in further investigations.

scholarly journals Antiinflammatory Potential and Fatty Acid Content of Lipophilic Leaf Extracts of Four Staphylea L. Species

2009 ◽  
Vol 4 (4) ◽  
pp. 1934578X0900400
Author(s):  
Lubica Lacikova ◽  
Eva-Maria Pferschy-Wenzig ◽  
Irena Masterova ◽  
Daniel Grancai ◽  
Rudolf Bauer
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Acid Content ◽  
Unsaturated Fatty Acids ◽  
Fatty Acid Content ◽  
In Vitro Assays ◽  
Leaf Extracts ◽  
Cox 2 ◽  
Cox 1

Staphylea preparations are used in TCM and have been used by native Americans for a number of indications, such as rheumatism. Based on this knowledge, the anti-inflammatory activity of light petroleum extracts of leaves of Staphylea colchica Stev., S. elegans Zab., S. holocarpa Hemsl. and S. pinnata L. has been determined using in vitro assays for inhibition of cyclooxygenase (COX-1 and COX-2) and leukotriene B4 (LTB4) formation by lipoxygenase (5-LOX). All extracts inhibited COX-1 and COX-2, with S. holocarpa and S. elegans performing best. Inhibition of LTB4 formation was less pronounced. As unsaturated fatty acids are known to inhibit arachidonic acid metabolism in vitro, the fatty acid content was determined of the active extracts and set in correlation with their activity. Unsaturated fatty acids were found to contribute to the observed COX-2 and LTB4 formation inhibitory activity to a different extent.

scholarly journals Chemical profiling, in vitro antioxidant, membrane stabilizing and antimicrobial properties of wild growing Murraya paniculata from Amarkantak (M.P.)

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Shruti Sonter ◽  
Shringika Mishra ◽  
Manish Kumar Dwivedi ◽  
Prashant Kumar Singh
Keyword(s):  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Hexane Extract ◽  
Phytochemical Analysis ◽  
Hypotonic Solution ◽  
High Concentration ◽  
Leaf Extracts ◽  
Traditional Use ◽  
Murraya Paniculata

AbstractThe excessive usage of antibiotics in humans and veterinary medicine has lead to the emergence of antibiotic resistance and now requires the use of novel antibiotics. There has been increased interest towards plants as source of drugs because of their pharmacological potency and long traditional usage. The aim of the current study was to evaluate bioactive components, antioxidant, and anti-inflammatory activities of the leaf extracts of Murraya paniculata, a plant traditionally used in Indian medicinal system. Evaluations were made for phytochemical analysis, antioxidant, membrane stabilizing, and antimicrobial activities. The methanol extract displayed the highest flavonoid and phenolic content, the acetone extract demonstrated considerable ABTS inhibitory activity (IC50value:555.18 ± 1.68 µg/mL) and the hexane extract exhibited highest H2O2 radical scavenging activity (IC50value: 509.84 ± 3.03 µg/mL). The aqueous extract displayed 19.4 ± 0.66% RBC hemolysis and 80.5 ± 0.66% protection caused by hypotonic solution at high concentration of the extract. The fractions of hexane extract revealed a higher zone of inhibition than crude extract. The major components found in the fractions were cyclohexane (40.11%) and 3-(6-Methoxy-3-methyl-2-benzofuranyl) Cyclohexanone (13.68%) as analyzed by GC–MS/MS technique. The current results validate the traditional use of the M. paniculata and warrant its potential in drug development programs in further investigations.

scholarly journals FRACTIONATION OF THE N-HEXANE EXTRACT OF GARCINIA BANCANA MIQ. (MANGGIS HUTAN) LEAVES AND ITS ANTIOXIDANT ACTIVITY BASED ON 1,1-DIPHENYL-2- PICRYLHYDRAZYL AND FERRIC REDUCING ANTIOXIDANT POWER ASSAYS

2018 ◽  
Vol 10 (1) ◽  
pp. 407
Author(s):  
Dewi Kumala Putri ◽  
Berna Elya ◽  
Nuraini Puspitasari
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Hexane Extract ◽  
Phytochemical Screening ◽  
Active Fraction ◽  
Leaf Extracts ◽  
Antioxidant Power ◽  
Dpph Method ◽  
Ferric Reducing Antioxidant Power

Objective: To assess the antioxidant activity from another part of the plant, in this study, leaf extracts in n-hexane were fractionated.Methods: Ten fractions were obtained and tested in vitro for antioxidant activity using two methods, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ferricreducing antioxidant power (FRAP), to identify the most active fraction.Results: The IC50 of the most active fraction was 36.24 μg/mL using the DPPH method, and the EC50 was 39.54 μg/mL using the FRAP method. Themost active fraction was also shown to contain terpenoids.Conclusion: The most active fraction of an n-hexane extract of the leaves of Gacinia bancana Miq., which was tested by both DPPH and FRAP methodshad antioxidant activities with IC50 and EC50 values of 36.2482 μg/mL and 39.5442 μg/mL, respectively. Phytochemical screening showed that activefraction contains terpenoids.

Inhibition of binding of gonadal steroids to serum binding proteins by non-esterified fatty acids: the influence of chain length and degree of unsaturation

1989 ◽  
Vol 120 (2) ◽  
pp. 175-179 ◽  
Author(s):  
C. Street ◽  
R. J. S. Howell ◽  
L. Perry ◽  
S. Al-Othman ◽  
T. Chard
Keyword(s):  
Fatty Acids ◽  
Protein Binding ◽  
Unsaturated Fatty Acids ◽  
Late Pregnancy ◽  
Sex Hormone Binding Globulin ◽  
Partial Purification ◽  
Normal Female ◽  
Esterified Fatty Acids ◽  
Vitro Binding

Abstract. The effect of non-esterified fatty acids (NEFA) on the in vitro binding of testosterone, 5-alpha dihydrotestosterone and estradiol E2 to sex hormone binding globulin (SHBG) was examined using pooled normal female serum, and SHBG and albumin fractions obtained from the partial purification of late pregnancy serum. A range of saturated and unsaturated fatty acids were examined for their effect on steroid-protein binding. In normal female serum, NEFA added at physiological concentrations disrupted steroid-protein binding. The shorter chain (C8–C12) saturated acids and the poly-unsaturated acids proved to be more effective inhibitors than the longer chain saturated or mono-unsaturated acids. The greatest inhibition was obtained with E2 whereas the binding of dihydrotestosterone was least affected. With partially purified SHBG, the same concentrations of NEFA were less effective at inhibiting the binding of dihydrotestosterone and testosterone but elicited the same effect with E2. The binding of steroids to albumin appeared to be unaffected by these concentrations of NEFA.

scholarly journals DETECTION OF BIOACTIVE FRACTIONS OF JUSTICIA ADHATODA L. LEAVES

2013 ◽  
Vol 3 ◽  
pp. 388
Author(s):  
Faiza Rasheed ◽  
Keyword(s):  
Crude Oil ◽  
Metal Ions ◽  
Dietary Fiber ◽  
Total Alkaloid ◽  
Antioxidant Activities ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Essential Metal ◽  
Justicia Adhatoda

In vitro antibacterial and antioxidant activities of various leaf extracts of Justicia adhatoda L. (locally known as Bhaikar) were assessed. The leaves were also subjected to various phytochemical analysis. Results revealed that leaves of J. adhatoda L. contain significant amount of total alkaloid, phenols flavonoid, saponins, tannins , protein, crude oil, dietary fiber, essential and non essential metal ions. The methanol, ethanol, butanol, chloroform and n-hexane leaf extracts of J

scholarly journals Quantitative Analysis of Nutrients and Biochemical Evaluation in Pomfret Fish (Pampus argenteus) During Frozen Storage

2021 ◽  
Vol 8 (3) ◽  
Author(s):  
Utkalendu suvendusekhar Samantaray ◽  
Swagatika Tripathy
Keyword(s):  
Oxidative Stress ◽  
Fatty Acids ◽  
Unsaturated Fatty Acids ◽  
Frozen Storage ◽  
Storage Condition ◽  
Essential Amino Acids ◽  
Omega 3 ◽  
High Concentration ◽  
Balanced Diet ◽  
Pampus Argenteus

Marine fish are well-known for being a high-quality protein source having high concentration of essential amino acids. It has high concentration of mono unsaturated and poly unsaturated fatty acids, which may aid in the optimization of lipid profiles and the reduction of the risk of coronary heart disease (CHD). The goal of this study was to estimate the nutritional and biochemical status of raw sea fish Pampus argenteus after 30 days of frozen storage at -200C with 15-day intervals. Nutrient study showed a decrease in protein and lipid content. The changes of hydrogen peroxide and oxidized lipid products were estimated in the muscle tissue during fresh and storage condition. Results indicate that during storage the oxidative stress increased. An antioxidant enzyme (superoxide dismutase, smutase, catalase, glutathione peroxidase) measurement was determined. The increased amount of oxidative stress during fish storage is shown by the differential activity of antioxidant enzymes. The amount of protein in fish varies slightly between species and even within species. Fish is high in protein, vitamins, minerals, and omega-3 fatty acids, which are essential for brain development (Spencer et al., 1971; Jacylin et al., 2010). A well-balanced diet consists variety of fish that can help in children's growth and development as well as their heart health (Jinadasa, 2014).

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