scholarly journals KvLEA, a New Isolated Late Embryogenesis Abundant Protein Gene fromKosteletzkya virginicaResponding to Multiabiotic Stresses

2016 ◽  
Vol 2016 ◽  
pp. 1-10 ◽  
Author(s):  
Xiaoli Tang ◽  
Hongyan Wang ◽  
Liye Chu ◽  
Hongbo Shao
Keyword(s):  
Abiotic Stresses ◽  
Stress Proteins ◽  
Bioinformatic Analysis ◽  
Late Embryogenesis Abundant ◽  
Late Embryogenesis Abundant Protein ◽  
Lea Proteins ◽  
Lea Gene ◽  
Late Embryogenesis ◽  
Kosteletzkya Virginica ◽  
Multiple Sequences

The LEA proteins are a kind of hydrophilic proteins, playing main functions in desiccation tolerance. However, their importance as a kind of stress proteins in abiotic stress is being clarified little by little. In this study we isolated, cloned, and identified the firstKvLEAgene inKosteletzkya virginica. Bioinformatic analysis showed that the protein encoded by this gene had common properties of LEA proteins and the multiple sequences alignment and phylogenetic analysis further showed that this protein had high homology with twoArabidopsisLEA proteins. Gene expression analysis revealed that this gene had a higher expression in root and it was induced obviously by salt stress. Moreover, the transcripts ofKvLEAwere also induced by other abiotic stresses including drought, high temperature, chilling, and ABA treatment. Among these abiotic stresses, ABA treatment brought about the biggest changes to this gene. Collectively, our research discovered a novel LEA gene and uncovered its involvement in multiabiotic stresses inK. virginica. This research not only enriched studies on LEA gene in plant but also would accelerate more studies onK. virginicain the future.

scholarly journals Metal-binding polymorphism in late embryogenesis abundant protein AtLEA4-5, an intrinsically disordered protein

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e4930 ◽  
Author(s):  
Leidys French-Pacheco ◽  
Cesar L. Cuevas-Velazquez ◽  
Lina Rivillas-Acevedo ◽  
Alejandra A. Covarrubias ◽  
Carlos Amero
Keyword(s):  
Metal Ions ◽  
Metal Ion ◽  
Intrinsically Disordered Protein ◽  
Late Embryogenesis Abundant ◽  
Terminal Region ◽  
Late Embryogenesis Abundant Protein ◽  
Lea Proteins ◽  
Intrinsically Disordered ◽  
Disordered Protein ◽  
Late Embryogenesis

Late embryogenesis abundant (LEA) proteins accumulate in plants during adverse conditions and their main attributed function is to confer tolerance to stress. One of the deleterious effects of the adverse environment is the accumulation of metal ions to levels that generate reactive oxygen species, compromising the survival of cells. AtLEA4-5, a member of group 4 of LEAs in Arabidopsis, is an intrinsically disordered protein. It has been shown that their N-terminal region is able to undergo transitions to partially folded states and prevent the inactivation of enzymes. We have characterized metal ion binding to AtLEA4-5 by circular dichroism, electronic absorbance spectroscopy (UV–vis), electron paramagnetic resonance, dynamic light scattering, and isothermal titration calorimetry. The data shows that AtLEA4-5 contains a single binding site for Ni(II), while Zn(II) and Cu(II) have multiple binding sites and promote oligomerization. The Cu(II) interacts preferentially with histidine residues mostly located in the C-terminal region with moderate affinity and different coordination modes. These results and the lack of a stable secondary structure formation indicate that an ensemble of conformations remains accessible to the metal for binding, suggesting the formation of a fuzzy complex. Our results support the multifunctionality of LEA proteins and suggest that the C-terminal region of AtLEA4-5 could be responsible for antioxidant activity, scavenging metal ions under stress conditions while the N-terminal could function as a chaperone.

scholarly journals A LEA Gene from a Vietnamese Maize Landrace Can Enhance the Drought Tolerance of Transgenic Maize and Tobacco

Agronomy ◽  
2019 ◽  
Vol 9 (2) ◽  
pp. 62 ◽  
Author(s):  
Bui Minh ◽  
Nguyen Linh ◽  
Ha Hanh ◽  
Le Hien ◽  
Nguyen Thang ◽  
...  
Keyword(s):  
Drought Tolerance ◽  
Late Embryogenesis Abundant ◽  
Transgenic Maize ◽  
Lea Proteins ◽  
Hydrophobic Residues ◽  
Maize Cultivars ◽  
Drought Tolerant ◽  
Lea Gene ◽  
Late Embryogenesis ◽  
Increasing Demand

Maize (Zea mays) is a major cereal crop worldwide, and there is increasing demand for maize cultivars with enhanced tolerance to desiccation. Late embryogenesis abundant (LEA) proteins group 5C is involved in plants’ responses to various osmotic stresses such as drought and salt. A putative group 5C LEA gene from Z. mays cv. Tevang 1 was isolated, named ZmLEA14tv, and cloned into a T-DNA for expression in plants. The deduced amino acid of ZmLEA14tv showed a conserved Pfam LEA_2 domain and a high proportion of hydrophobic residues, characteristic of group 5C LEA proteins. Transgenic tobacco and maize plants expressing ZmLEA14tv were generated. During drought simulation conditions, the ZmLEA14tv-expressing plants of tobacco showed improved recovery ability, while those of maize enhanced the seed germination in comparison with the non-transgenic control plants. In addition, the survival rate of ZmLEA14tv transgenic maize seedlings was twice as high as the control. These results indicated that ZmLEA14tv might be involved in the drought tolerance of plants and could be a candidate gene for developing enhanced drought-tolerant crops.

scholarly journals Overexpression of ZmDHN11 could enhance transgenic yeast and tobacco tolerance to osmotic stress

2021 ◽  
Author(s):  
huining Ju ◽  
daxing Li ◽  
Dequan Li ◽  
Xinghong Yang ◽  
Yang Liu
Keyword(s):  
Osmotic Stress ◽  
Stress Proteins ◽  
Plant Stress ◽  
Late Embryogenesis Abundant ◽  
Water Deficit Stress ◽  
Lea Proteins ◽  
Lea Protein ◽  
Transgenic Yeast ◽  
Late Embryogenesis ◽  
Group Ii

Abstract Late embryogenesis abundant (LEA) proteins are widely assumed to play crucial roles in environmental stress tolerance, but their function has remained obscure. Dehydrins are group II LEA proteins, which are highly hydrophilic plant stress proteins. In the present study, a novel group II LEA protein, ZmDHN11 was cloned and identified from maize. The expression of ZmDHN11 was induced by high osmotic stress, low temperature, salinity and ABA (abscisic acid). The ZmDHN11 protein specifically accumulated in the nuclei and cytosol. Further study indicated that ZmDHN11 is phosphorylated by the casein kinase CKII. ZmDHN11 protected the activity of LDH under water deficit stress. The overexpression of ZmDHN11 endows transgenic yeast and tobacco with tolerance to osmotic stress.

scholarly journals Identification and Expression Analysis of Stress Responsive Genes in Lentil (Lens culinaris)

2020 ◽  
pp. 24-34
Author(s):  
Annu Yadav ◽  
Himanshi . ◽  
Shruti . ◽  
Jitender Singh ◽  
Pankaj Kumar ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Salinity Stress ◽  
Expression Analysis ◽  
Abiotic Stresses ◽  
Stress Proteins ◽  
Late Embryogenesis Abundant ◽  
Lea Gene ◽  
Adverse Environmental Conditions ◽  
Periodic Stress

Plants during their growth, experience periodic stress conditions both abiotic (adverse environmental conditions) as well as biotic (infection by pathogens). They appear to respond to these adverse conditions by modulating the expression of many genes. One of the pronounced effects of stress on plant is the enhanced synthesis of a set of proteins-termed ' stress proteins'. Lentil contains asset of genes/proteins which helps this crop to overcome abiotic stresses. In the present study, HSP70 (Heat Shock Protein), LEA (Late Embryogenesis Abundant) and Aldolase genes were identified and cloned in pTZ57RT vector followed by sequencing. Expression analysis was done through Q-PCR which was assessed by using cDNA from all the heat, drought and salinity stressed and unstressed lentil cotyledons. The highest level of transcript of HSP70 was realized upon exposure to heat at 45°C for 3 hour followed by at 45°C for 2 hour and lowest at 40°C for 1hour. LEA gene was identified under drought and salinity stress and highest transcript was at 20% PEG for 3 hour (drought stress) and in salinity stress highest transcript was at 150 mm for 6 hour.  For Aldolase gene highest transcript was recorded after 3, 6 and 12 hr at 100 mM, 150 mM, 200 mM of salinity stress respectively.  From these studies it can be concluded that heat shock protein gene, LEA, and aldolase present in lentil which can be exploited in overcoming the abiotic stresses for obtaining the higher productivity in crop plants through genetic engineering.

scholarly journals Genome-wide identification and expression analysis of late embryogenesis abundant protein-encoding genes in rye (Secale cereale L.)

PLoS ONE ◽  
2021 ◽  
Vol 16 (4) ◽  
pp. e0249757
Author(s):  
Mengyue Ding ◽  
Lijian Wang ◽  
Weimin Zhan ◽  
Guanghua Sun ◽  
Xiaolin Jia ◽  
...  
Keyword(s):  
Secale Cereale ◽  
Abiotic Stresses ◽  
Late Embryogenesis Abundant ◽  
Late Embryogenesis Abundant Protein ◽  
Pcr Analysis ◽  
Genome Wide ◽  
Late Embryogenesis ◽  
Secale Cereale L ◽  
Peg Treatment ◽  
Spatial Specificity

Late embryogenesis abundant (LEA) proteins are members of a large and highly diverse family that play critical roles in protecting cells from abiotic stresses and maintaining plant growth and development. However, the identification and biological function of genes of Secale cereale LEA (ScLEA) have been rarely reported. In this study, we identified 112 ScLEA genes, which can be divided into eight groups and are evenly distributed on all rye chromosomes. Structure analysis revealed that members of the same group tend to be highly conserved. We identified 12 pairs of tandem duplication genes and 19 pairs of segmental duplication genes, which may be an expansion way of LEA gene family. Expression profiling analysis revealed obvious temporal and spatial specificity of ScLEA gene expression, with the highest expression levels observed in grains. According to the qRT-PCR analysis, selected ScLEA genes were regulated by various abiotic stresses, especially PEG treatment, decreased temperature, and blue light. Taken together, our results provide a reference for further functional analysis and potential utilization of the ScLEA genes in improving stress tolerance of crops.

Desiccation Dependent Structure and Stability of an Anhydrobiotic Nematode Late Embryogenesis Abundant (LEA) Protein

2009 ◽  
Author(s):  
Dai-xi Li ◽  
Xiaoming He
Keyword(s):  
Water Deficit ◽  
Structural Stability ◽  
Stress Proteins ◽  
Late Embryogenesis Abundant ◽  
Lea Proteins ◽  
Lea Protein ◽  
Late Embryogenesis ◽  
Severe Water Deficit ◽  
Space Filler ◽  
Group 3

A number of organisms have been found to be capable of surviving severe water deficit as a result of extreme drought and cold in nature by entering a state of suspended animation (i.e., anhydrobiosis or life without water) [1]. Although the precise molecular repertoire of desiccation tolerance in anhydrobiotic organisms is still not fully understood, results from recent studies indicate the crucial role of stress proteins such as the late embryogenesis abundant (LEA) proteins [2]. LEA proteins have been proposed to play a variety of roles in protecting biologicals from damaging by dehydration stress such as molecular chaperone and shield, ion chelator, antioxidant, and space filler. The multifunctional capacity of LEA proteins has been attributed in part to their structural plasticity: they are unfolded and when fully hydrated and become folded during water deficit [1]. However, the structural stability of LEA protein in response to desiccation is still not fully understood. In this study, the structure alteration of a group 3 LEA protein from an anhydrobiotic nematode (AavLEA1) [2] were investigated using the molecular dynamics (MD) simulation approach to understand the structural stability at different water contents.

scholarly journals Genome-wide search and structural and functional analyses for late embryogenesis-abundant (LEA) gene family in poplar

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Zihan Cheng ◽  
Xuemei Zhang ◽  
Wenjing Yao ◽  
Kai Zhao ◽  
Lin Liu ◽  
...  
Keyword(s):  
Gene Family ◽  
Higher Plants ◽  
Abiotic Stress Tolerance ◽  
Gene Families ◽  
Regulatory Elements ◽  
Late Embryogenesis Abundant ◽  
Genome Wide ◽  
Lea Gene ◽  
Late Embryogenesis ◽  
Genome Wide Search

Abstract Background The Late Embryogenesis-Abundant (LEA) gene families, which play significant roles in regulation of tolerance to abiotic stresses, widely exist in higher plants. Poplar is a tree species that has important ecological and economic values. But systematic studies on the gene family have not been reported yet in poplar. Results On the basis of genome-wide search, we identified 88 LEA genes from Populus trichocarpa and renamed them as PtrLEA. The PtrLEA genes have fewer introns, and their promoters contain more cis-regulatory elements related to abiotic stress tolerance. Our results from comparative genomics indicated that the PtrLEA genes are conserved and homologous to related genes in other species, such as Eucalyptus robusta, Solanum lycopersicum and Arabidopsis. Using RNA-Seq data collected from poplar under two conditions (with and without salt treatment), we detected 24, 22 and 19 differentially expressed genes (DEGs) in roots, stems and leaves, respectively. Then we performed spatiotemporal expression analysis of the four up-regulated DEGs shared by the tissues, constructed gene co-expression-based networks, and investigated gene function annotations. Conclusion Lines of evidence indicated that the PtrLEA genes play significant roles in poplar growth and development, as well as in responses to salt stress.

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