scholarly journals Anticancer Activity, Antioxidant Activity, and Phenolic and Flavonoids Content of Wild Tragopogon porrifolius Plant Extracts

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Fuad Al-Rimawi ◽  
Suzi Rishmawi ◽  
Sharehan H. Ariqat ◽  
Mahmoud F. Khalid ◽  
Ismail Warad ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Anticancer Activity ◽  
Cell Lines ◽  
Plant Extracts ◽  
Folk Medicine ◽  
Reducing Power ◽  
Total Phenolic ◽  
Osteosarcoma Cell ◽  
Anticancer Activities ◽  
Antioxidant Power

Tragopogon porrifolius, commonly referred to as white salsify, is an edible herb used in folk medicine to treat cancer. Samples of Tragopogon porrifolius plant grown wild in Palestine were extracted with different solvents: water, 80% ethanol, and 100% ethanol. The extracts were analyzed for their total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activity (AA). Four different antioxidant assays were used to evaluate AA of the extracts: two measures the reducing power of the extracts (ferric reducing antioxidant power (FRAP) and cupric reducing antioxidant power (CUPRAC)), while two other assays measure the scavenging ability of the extracts (2,2-azino-di-(3-ethylbenzothialozine-sulphonic acid (ABTS)) and 2,2-diphenyl-1-picrylhydrazyl (DPPH)). Anticancer activity of the plant extracts were also tested on HOS and KHOS osteosarcoma cell lines. The results revealed that the polarity of the extraction solvent affects the TPC, TFC, and AA. It was found that both TPC and AA are highest for plant extracted with 80% ethanol, followed by water, and finally with 100% ethanol. TFC however was the highest in the following order: 80% ethanol > 100% ethanol > water. The plant extracts showed anticancer activities against KHOS cancer cell lines; they reduced total cell count and induced cell death in a drastic manner.

scholarly journals Antioxidant and Anticancer Activities of Wampee (Clausena lansium(Lour.) Skeels) Peel

2009 ◽  
Vol 2009 ◽  
pp. 1-6 ◽  
Author(s):  
K. Nagendra Prasad ◽  
Jing Hao ◽  
Chun Yi ◽  
Dandan Zhang ◽  
Shengxiang Qiu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Liver Carcinoma ◽  
Scavenging Activity ◽  
Anticancer Activities

Antioxidant activities of wampee peel extracts using five different solvents (ethanol, hexane, ethyl acetate, butanol and water) were determined by using in-vitro antioxidant models including total antioxidant capability, 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging activity, reducing power, and superoxide scavenging activity. Ethyl acetate fraction (EAF) exhibited the highest antioxidant activity compared to other fractions, even higher than synthetic antioxidant butylated hydroxyl toluene (BHT). In addition, the EAF exhibited strong anticancer activities against human gastric carcinoma (SGC-7901), human hepatocellular liver carcinoma (HepG-2) and human lung adenocarcinoma (A-549) cancer cell lines, higher than cisplatin, a conventional anticancer drug. The total phenolic content of wampee fraction was positively correlated with the antioxidant activity. This is the first report on the antioxidant and anticancer activities of the wampee peel extract. Thus, wampee peel can be used potentially as a readily accessible source of natural antioxidants and a possible pharmaceutical supplement.

scholarly journals Antioxidant and cytotoxic studies of Acacia nilotica twig extract and their green synthesized silver nanoparticles

2020 ◽  
Vol 9 (2) ◽  
pp. 975-980 ◽  
Keyword(s):  
Antioxidant Activity ◽  
Silver Nanoparticles ◽  
Cell Lines ◽  
Antioxidant Activities ◽  
Reducing Power ◽  
Acacia Nilotica ◽  
Total Phenolic ◽  
Hek 293 ◽  
Selected Area Electron Diffraction ◽  
Wide Range

Acacia nilotica (L.) Delile is well known as “Desi Kikar”or Babul in India that possesses a wide range of pharmacological activities. In the present study, Acacia nilotica twig extract and its synthesized silver nanoparticles (AgNPs) were evaluated for total phenolic content (TPC), antioxidant activity and cytotoxic effects. Characterization of AgNPs was done by UV-Visible spectroscopy, Fourier-transform infrared spectroscopy (FTIR), Transmission electron microscopy (TEM) and Selected area electron diffraction (SAED) techniques. Antioxidant potential was determined using different assays including 2,2-diphenyl-1-picrylhydrazyl (DPPH), reducing power and β-carotene linoleic acid. Cytotoxicity was tested by 3-(4,5-dimethyl-2-yl)-2,5-diphynyl tetrazolium bromide (MTT) assay on Human embryonic kidney (HEK)-293 cell lines. The results indicated that AgNPs exhibited higher antioxidant activity (81.11 %) and TPC (57.35 mg of GAE/mL of extract) as compare to plant extract. A positive correlation was observed between the TPC and antioxidant activities. The inhibitory concentration (IC50) of A. nilotica extract and AgNPs was 52.08µg/mL and 56.82µg/mL respectively. Cytotoxicity against HEK-293 cell lines was dose dependent. Accordingly, it is summarized that A. nilotica based AgNPs could serve as a potential antioxidant for therapeutic purposes.

scholarly journals Increased Phenolic Content and Enhanced Antioxidant Activity in Fermented Glutinous Rice Supplemented with Fu Brick Tea

Molecules ◽  
2019 ◽  
Vol 24 (4) ◽  
pp. 671 ◽  
Author(s):  
Xiao Xu ◽  
Wenxiu Hu ◽  
Siduo Zhou ◽  
Chuanhai Tu ◽  
Xiudong Xia ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Hydroxyl Radical ◽  
Phenolic Content ◽  
Reducing Power ◽  
Total Phenolic ◽  
Food Ingredient ◽  
Antioxidant Power ◽  
Functional Activities ◽  
Glutinous Rice

Glutinous rice-based foods have a long history are consumed worldwide. They are also in great demand for the pursuit of novel sensory and natural health benefits. In this study, we developed a novel fermented glutinous rice product with the supplementation of Fu brick tea. Using in vitro antioxidant evaluation and phenolic compounds analysis, fermentation with Fu brick tea increased the total phenolic content and enhanced the antioxidant activity of glutinous rice, including scavenging of 1,1-Diphenyl-2-picryl-hydrazyl (DPPH) radical, 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) radical, and hydroxyl radical, ferric-reducing antioxidant power, and ferric ion reducing power and iron chelating capability. Besides, compared with traditional fermented glutinous rice, this novel functional food exhibited a stronger activity for protecting DNA against hydroxyl radical-induced oxidation damage. Quantitative analysis by HPLC identified 14 compounds covering catechins and phenolic acids, which were considered to be positively related to the enhanced antioxidant capability. Furthermore, we found that 80% ethanol was a suitable extract solvent compared with water, because of its higher extraction efficiency and stronger functional activities. Our results suggested that this novel fermented glutinous rice could serve as a nutraceutical food/ingredient with special sensory and functional activities.

scholarly journals Papaver Decaisnei: GC-MS Alkaloids Profiling, in Vitro Antioxidant, and Anticancer Activity

2022 ◽  
Author(s):  
Ahmed Aj.Jabbar ◽  
Fuad Othman Abdullah ◽  
Kamaran Kaiani Abdulrahman ◽  
yaseen Galali ◽  
Abdullah Sh. Sardar
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Phytochemical Analysis ◽  
Endemic Plant ◽  
Anticancer Activities ◽  
Antioxidant Power ◽  
Leaves And Roots ◽  
Mcf 7

Abstract The Papaver L. plant have been well known as a source of pharmaceutically valuable alkaloids (noscapine, thebaine, codeine, roemerine, papaverine and morphine). The current study investigates the phytochemical, in-vitro antioxidant, and anticancer activities of papaver decaisnei, an endemic plant species to the flora of Kurdistan-Iraq. The chemical analysis of the methanolic (MeOH) extracts of flowers, leaves, and roots of papaver decaisnei were made by using gas chromatography-mass spectrophotometry (GC-MS), and the antioxidant activity evaluation done by radical scavenging [on 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2j-azino-bis (3- ethylbenzothiazoline-6-sulfonic acid) (ABTS)], and reducing power [cupric reducing antioxidant capacity (CUPRAC), and ferric reducing antioxidant power (FRAP)] assays. The anticancer actions were presented as IC50 (inhibitory concentration at 50%) on human colorectal adenocarcinoma (Caco-2), mammary cancer cells (MCF-7), and human cervical carcinoma (HeLa) cells. The results of the phytochemical analysis showed 17, 19, and 22 chemical compounds for flowers, leaves, and roots of P. decaisnei, respectively. The prevalent organic compounds of P. decaisnei were alkaloids, phenolics, fatty acids, esters, and phytosterols, namely Roemerine (70.44%), Decarbomethoxytabersonine, 9,12,15-Octadecatrien-1-ol, Hexadecanoic acid, 6,8-Dioxa-3-thiabicyclo(3,2,1)octane 3,3-dioxide, and γ-Sitosterol. The antioxidant activity of plant organ extracts was within 39.1-143.5 μg/ml for DPPH and 123.12-276.4 μg/ml for ABTS assays, while, the FRAP and CUPRAC values ranged within 12.4- 34.3 and 42.6-75.8 μg/ml, respectively. The anticancer action of P.decaisnei organ extracts was found against all tested human cell lines (Caco-2, MCF-7, HeLa) with inhibitory concentrations (IC50) values between 125.3-388.4 μg/ml. The presented data on alkaloid contents and biological activity of P. decaisnei can serve a ground knowledge for the future biomedical synthesis and cancer research projects.

Disparities of phytochemical constituents and antioxidant activities of some Indigofera species

2021 ◽  
pp. 1-10
Author(s):  
Firdose R. Kolar ◽  
Sidanand V. Kambhar ◽  
Manjula Chavan ◽  
Shruti Kadam ◽  
Peerambi Nadaf
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Plant Material ◽  
Pearson Correlation ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Phytochemical Constituents

Abstract The current study was intended to examine the difference in the phytochemical and antioxidant profile of nine Indigofera species. With this aim, the species were assessed for total phenolic content, flavonoid content, and antioxidant activity using ferric reducing antioxidant power (FRAP), DPPH free radical scavenging, ferrous ion chelating activity, phosphomolybdenum reducing power, deoxyribose degradation, β-carotene bleaching, nitric oxide scavenging, and superoxide radical scavenging assays. The content of total phenolics and flavonoids ranged from 1.33 to 22.5 mg TAE/g plant material and 0.47 to 5.02 mg QE/g plant material respectively in the various species, while the antioxidant activity as tested with different antioxidant test models varied with the species studied as well as with the solvents used for the extraction. Interestingly, all the tested extracts demonstrated considerable free radical scavenging activity, moreover, I. hirsuta, I. glandulosa, I. linnaei, I. hochstetteri, I. linifolia, and I. trita were found to be the most effective among the species studied. The results indicated the significant differences in phytochemical constituents and the antioxidant activity among the species. The Pearson correlation coefficient analysis exhibited a significant correlation of phenolics and flavonoids with the antioxidant activity.

Antioxidant and Phenolic Characterization with HPLC of Various Extract of Verbascum glomeratum Linneus

2020 ◽  
Vol 42 (2) ◽  
pp. 222-222
Author(s):  
i dem Aydin i dem Aydin ◽  
Akgul Rakhimzhanova Akgul Rakhimzhanova ◽  
zge Kilin arslan and Ramazan Mammadov zge Kilin arslan and Ramazan Mammadov
Keyword(s):  
Antioxidant Activity ◽  
Linoleic Acid ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Phenolic Contents ◽  
Phenolic Constituents ◽  
Ethanol Extracts ◽  
Β Carotene

Verbascum L. species have been used since ancient times in traditional medicine thanks to their bioactive compounds. In this study, we evaluated the antioxidant activity and phenolic contents of ethanolic and acetonic extract of Verbascum glomeratum that collected from Denizli, Turkey. The antioxidant activity were evaluated by using various methods (β-carotene/linoleic acid, ferric reducing power assays (FRAP), radical scavenging assays of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2and#39;-azino-bis-3-ethyl benzthiazoline-6-sulphonic acid (ABTS), phoshomolibdenum methods) and total phenolic, flavonoid and tannin contents in the extracts were determined. Antioxidant activity was determined as β-carotene/linoleic acid (%58.5and#177;0.021), DPPH (IC50: 1.220and#177;0.025) and ABTS (IC50:1.552and#177;0.021), ferric reducing antioxidant power (FRAP) (4.151and#177;0.056 mgTE/g), phoshomolibdenum assay (8.218and#177;0.015 mgAE/g) total phenolic, total flavonoid and tannin contents was found as (4.41and#177;0.007 mgGAE/mL), (87.142and#177;0.009 and#181;QE/g), and (30.178and#177;0.008 mgCEs/g) respectively, and then 14 different phenolic compounds in ethanol extracts was carried out by HPLC. Epicatechin (2742.09 and#181;g/g) and 2,5dihidroksi (2544.96 and#181;g/g) were the most abundant phenolic constituents in the extracts. According the results, the extract of V. glomeratum may be considered as a potential source of biological agents.

scholarly journals Evaluation of in vitro Antioxidant and Cytotoxic Effect of Methanol Extract from aerial parts of Myristica beddomei King ssp. ustulata W.J. de Wilde

2020 ◽  
pp. 115-121
Author(s):  
Neethu Joy ◽  
Mahesh Mohan
Keyword(s):  
Antioxidant Activity ◽  
Anticancer Activity ◽  
Cytotoxic Effect ◽  
Total Phenolic ◽  
Ovarian Cancer Cells ◽  
Cytotoxicity Test ◽  
Antioxidant Power ◽  
Ic50 Value ◽  
Dpph Method

Plants are traditionally used for pharmacological activities because of its ability to produce bioactive compounds. Myristica beddomei King ssp. ustulata W.J. de Wilde is an ethnomedicinal plant and it is seen in South Western Ghats of Kerala, India. The present study assessed the phenolic content, flavonoid concentration, in vitro antioxidant and cytotoxic effect of different parts of Myristica beddomei King. The total phenolic contents in the extracts ranged from 96.29 (pericarp) to 314.47 (bark) mg g-1 gallic acid equivalent. The concentration of flavonoids in different plant part extracts ranged from 1.81   to 2.76 mg g-1 equivalent to quercetin. All the parts exhibited potential antioxidant activity with an IC50 value of 2.87 to 9.67 μg ml-1 when compared to the standard ascorbic acid with an IC50 value of 2 μg ml-1 in 1,1-diphenyl-2- picryl-hidrasil (DPPH) method. Bark showed highest activity in terms of DPPH radical scavenging (IC50 value of 2.87 µg ml-1), phosphomolybdenum test (2261.33 ± 1.65 mg g-1 trolox equivalent) and ferric ion reducing antioxidant power (FRAP) (113.1 ± 0.28 µmol Fe2+ µg-1) while pericarp showed low antioxidant activity. The in vitro screening results revealed that the seeds exhibited promising anticancer activity compared to PA1 (Ovarian Cancer) cells (50 % inhibition) were observed at a concentration 100.68 µg ml-1.  In cytotoxicity test L929 (Fibroblast) cell line compared to the other parts pericarp, mace and seed needed higher concentration (>240 µg ml-1) for LC50 value. It is a promising plant for further development of antioxidant agent as it got high content of phenolic compounds and potential antioxidant and anticancer activity.

scholarly journals Antioxidant and antiproliferative activities of the n-butanol extract of Centaurea maroccana Ball aerial parts

2021 ◽  
Vol 34 (1) ◽  
pp. 5-11
Author(s):  
Imen Aissous ◽  
Mouad Benrebai ◽  
Ercan Cacan ◽  
Berkel Caglar ◽  
Ramazan Erenler ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Cell Lines ◽  
Proliferative Activity ◽  
Homo Sapiens ◽  
Quantitative Estimation ◽  
Reducing Power ◽  
Aluminium Chloride ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
Butanolic Extract

Abstract The aim of the present study is to evaluate the total phenolic contents, antioxidant and anti-proliferative activities of the n-BuOH extract of Centaurea maroccana (BECM). The total phenolic and flavonoid of the butanolic extract of the plant were assessed by using Folin-Ciocalteau and aluminium chloride colorimetric assays, respectively, and the quantitative estimation of total flavonoids and phenols revealed the richness of the extract in these compounds. Antioxidant activity was evaluated using standard lab colorimetric methods, while the anti-proliferative activity was evaluated using sulforhodamine B (SRB) assay against C6 (Rattus norvegicus brain glioma) and HT29 (Homo sapiens colorectal adenocarcinoma) cell lines. The n-butanolic extract of Centaurea maroccana showed a strong antioxidant activity through DPPH, ABTS•+, DMSO alkalin, Reducing power, β-Carotene-linoleic acid and CUPRAC assays. Furthermore, the anti-proliferative activity against C6 and HT29 of Centaurea maroccana exhibited a high effect by the decrease in viability of both cancer cell lines. Our results suggest a possible use of Centaurea maroccana as a source of natural antioxidant and chemo-preventive agents against cancer.

scholarly journals Phytochemical Contents and Antioxidant Activity of Medicinal Plants from the Rubiaceae Family in Thailand

2021 ◽  
Vol 8 (1) ◽  
pp. 24-31
Author(s):  
Rungcharn Suksungworn ◽  
Sutsawat Duangsrisai
Keyword(s):  
Antioxidant Activity ◽  
Plant Extracts ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Antioxidant Power ◽  
Nitric Oxide Radical ◽  
Promising Source ◽  
Phytochemical Contents

Several plants of the Rubiaceae family possess potential pharmacological properties, such as antioxidant activity, for subsequent drug development. We investigated the methanolic extracts from the bark and wood of five Rubiaceae species for phenolic and flavonoid contents and antioxidant activity. Regarding the phytochemical contents and antioxidant activity, Mitragyna diversifolia wood (437.57 ± 9.90 mg GAE g-1) and Haldina cordifolia wood (30.11 ± 0.20 mg QE g-1) displayed the highest total phenolic content (TPC) and total flavonoid content (TFC) respectively. Morinda coreia bark followed the highest antioxidant activities (IC50 = 360.58 ± 19.28 µg ml-1) in the 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity (DPPH), Catunaregam tomentosa bark (IC50 = 13.96 ± 5.32 µg ml-1) in the nitric oxide radical scavenging activity (NO), M. coreia wood (IC50 = 918.27 ± 0.16 µg ml-1) in the superoxide radical scavenging activity (SO) and M. coreia wood (IC50 = 236.65 ± 1.66 µg ml-1) in ferric reducing antioxidant power activity (FRAP). The TPC and TFC displayed strong correlations with DPPH in M. diversifolia wood and with FRAP in M. diversifolia bark and wood. We found high correlation between TFC and FRAP in all plant extracts except C. tomentosa wood, while no relation was detected between TFC and NO in all plant extracts. Comparing Rubiaceae species, the highest antioxidant potential were showed in C. tomentosa bark. Overall, it is worth mentioning that the Rubiaceae species exhibit potential as a promising source of natural antioxidants.

scholarly journals Determination of Multiple Antioxidant Activities of Endemic Tricholoma anatolicum H.H Doğan & Intini Collected from Turkey

2018 ◽  
Vol 6 (11) ◽  
pp. 1582 ◽  
Author(s):  
Hatıra Taşkın ◽  
Tülin Eker ◽  
Fuat Bozok ◽  
Hasan Hüseyin Doğan ◽  
Saadet Büyükalaca
Keyword(s):  
Antioxidant Activity ◽  
Methanol Extract ◽  
Antioxidant Activities ◽  
Far East ◽  
Reducing Power ◽  
Total Phenolic ◽  
Mushroom Species ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power

Tricholoma anatolicum H.H. Doğan & Intini is known as the most prized mushroom species in Feke region of Turkey. This mushroom species is collected from Cedar (Cedrus libani) forests and therefore is named as Cedar mushroom in this region. It is collected and consumed by local collectors and also exported to Far East countries such as Japan by exporting companies. Therefore, it is important to investigate the nutritional and medical importance of this species. The aim of this study is to reveal the antioxidant activity of methanol extract in different concentrations (1, 2 and 4 mg/mL) of T. anatolicum H.H. Doğan & Intini collected from Feke district of Adana province of Turkey in 2015. In this study, it was determined that total phenolic content of this mushroom was 56 mg/kg. 2,2-diphenyl-1-picrylhydrazyl (DPPH), nitric oxide (NO) and reducing power (RP) activities given in trolox (µM) and ferric-reducing antioxidant power (FRAP) given in FeSO4 (µM) were found as 449, 180, 337 and 2 at the highest concentration (4 mg/mL), respectively. As a result, it could be suggested that methanol extract of T. anatolicum H.H. Doğan & Intini has significantly antioxidant activity.

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