scholarly journals Identifying Antioxidant and Antimicrobial Activities of the Phenolic Extracts and Mineral Contents of Virgin Olive Oils (Olea europaeaL. cv. Edincik Su) from Different Regions in Turkey

2016 ◽  
Vol 2016 ◽  
pp. 1-11 ◽  
Author(s):  
Ayhan Dağdelen
Keyword(s):  
Antimicrobial Effect ◽  
Antimicrobial Activities ◽  
Emission Spectrometry ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Mineral Contents ◽  
Virgin Olive Oils ◽  
Olive Oils ◽  
Antioxidant And Antimicrobial Activities ◽  
Phenolic Extracts

The objective of this study was to identify the antioxidant and antimicrobial activities of the phenolic extracts and mineral contents of virgin olive oils (Olea europaeaL. cv. Edincik Su) obtained from three different locations, Edincik, Gomec, and Izmir, in Turkey. Antioxidant activity was analysed spectrometrically, antimicrobial effect was evaluated by disc diffusion method, and mineral contents were determined by using Inductively Coupled Plasma-Optical Emission Spectrometry (ICP-OES). Total phenolic contents and free radical scavenging activities of Edincik Su olive cultivar were found between 159.99 and 189.64 mg gallic acid equivalent/kg and 21.76 and 31.79% inhibition of DPPH•radical values, respectively. Phenolic composition was also varied according to location and hydroxytyrosol and tyrosol were determined as major phenolic compounds. Fourteen pathogenic bacteria and two fungal strains were used to determine the antimicrobial activity. Phenolic extracts showed antimicrobial effect against a wide range of microorganisms and the descending rank of the antimicrobial activities was Gomec, Izmir, and Edincik samples. The highest antimicrobial activities were determined againstClostridium perfringensATCC 13124 andCampylobacter jejuniATCC  33291 in all samples. Sixteen elements were used as standards (eleven of them were heavy metals) to identify mineral contents. Fe, Mg, Sn, K, and Ca were determined in Gomec samples and Mg and K were detected in Edincik samples while no mineral content was detected in Izmir sample.

scholarly journals Comparative Insight upon Chitosan Solution and Chitosan Nanoparticles Application on the Phenolic Content, Antioxidant and Antimicrobial Activities of Individual Grape Components of Sousão Variety

Antioxidants ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 178
Author(s):  
Vanessa Silva ◽  
Rupesh Kumar Singh ◽  
Nelson Gomes ◽  
Bruno Gonçalves Soares ◽  
Adriana Silva ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Chitosan Nanoparticles ◽  
Chitosan Solution ◽  
Antimicrobial Activities ◽  
Added Value ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Resistant Bacteria ◽  
Pharmaceutical Industries ◽  
Antioxidant And Antimicrobial Activities

Chitosan, a natural polysaccharide, has been previously proposed as an elicitor in plants to prevent pathogen infections. The present study aimed to analyze the effect of chitosan solution and chitosan nanoparticles treatment applied on the grapevine variety Sousão with respect to the phenolic composition, antioxidant potential and antibacterial activity of its individual grape components. Grapevine plants of selected lines were sprayed with chitosan solution and chitosan nanoparticles, and ethanolic extracts of stems, seeds and skins were prepared from grapevines treated and not treated with chitosan. Total phenolic, anthocyanin and tannin contents were studied, and the identification of the individual phenolic compounds was performed by HPLC-DAD. The antimicrobial susceptibility method was performed using the Kirby-Bauer disc diffusion method against multidrug-resistant bacteria. Overall, there was small increase in the concentration of phenolic compounds, antioxidant and antimicrobial activities in grape components treated with chitosan solution. Seed extracts showed the highest antioxidant and antimicrobial activities. The studied individual components obtained from chitosan-treated grapevines could represent an added value due to the increased antioxidant and antibacterial potentials. The phenolic compounds found in components may be used in food and pharmaceutical industries as natural food preservers and antibiotic adjuvants.

scholarly journals Comparative Assessment on Selected Physicochemical Parameters and Antioxidant and Antimicrobial Activities of Honey Samples from Selected Districts of the Amhara and Tigray Regions, Ethiopia

2019 ◽  
Vol 2019 ◽  
pp. 1-10
Author(s):  
Mekuanint Lewoyehu ◽  
Meareg Amare
Keyword(s):  
Antioxidant Activity ◽  
Physicochemical Parameters ◽  
Antibacterial Activities ◽  
Antimicrobial Activities ◽  
Climatic Conditions ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
Production Areas ◽  
Antioxidant And Antimicrobial Activities

The composition and properties of natural honeys differ with plant species on which the bees forage and the climatic conditions of the production areas. In Ethiopia, Amhara and Tigray are neighboring regions consisting of different agricultural activities and blossoms from different types of vegetations which may influence the natural composition and hence the properties of honey. So, the aim of the current study was to assess the quality of honey from selected districts of the two regions. In the study, 18 composited honey samples were collected from six selected districts and analyzed for selected physicochemical parameters and antioxidant and antimicrobial activities. The analyses of physicochemical parameters were carried out following standard procedures of IHC and QSAE. The antioxidant activity was determined by analyzing the RSA using DPPH while the antibacterial activities were determined by the agar well diffusion method. The moisture, ash content, electrical conductivity, pH, free acidity, reducing sugar, and sucrose content of the honey samples were found to be in the range 16.34 ± 0.26 to 19.83 ± 0.43 %, 0.08 ± 0.00 to 0.45 ± 0.03 %, 0.19 ± 0.00 to 0.89 ± 0.03 mS/cm, 3.79 ± 0.04 to 4.20 ± 0.01, 19.56 ± 1.13 to 38.11 ± 1.54 meq/kg, 62.10 ± 0.48 to 66.37 ± 0.20 %, and 1.35 ± 0.08 to 5.96 ± 0.10 %, respectively. The total phenolic content ranged from 1165.60 ± 23.45 to 1854.83 ± 10.47 mg/kg with antioxidant activity of 21.64 ± 0.26 to 36.12 ± 0.52 AEAC/100 g. The total phenolic contents showed strong correlation with RSA. Furthermore, all honey samples showed an antibacterial activity varying from 23.23 ± 0.12 to 28.84 ± 0.24 mm.

scholarly journals Phenolic Contents, in vitro Antioxidant and Antimicrobial Activities of Genista microcephala Coss. & Dur.

2018 ◽  
Vol 5 (2) ◽  
pp. 8-22
Author(s):  
Djamila Maanani ◽  
Narimane Segueni ◽  
Salah Rhouati ◽  
Yavuz Selim Çakmak ◽  
Maltem Asan-Ozusaglam ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Total Phenolic ◽  
In Vitro Tests ◽  
Phenolic Contents ◽  
Significant Difference ◽  
Total Antioxidant ◽  
Antioxidant And Antimicrobial Activities

Abstract This study was carried out to determine the phenolic contents as well as to evaluate the antioxidant and antimicrobial activities of Genista microcephala. Total phenolic and flavonoid compounds were quantified. Antioxidant activity was evaluated by various in vitro tests, including Ferric Ion Reducing Activity (FRP), Cuprac Ion Reducing Antioxidant Capacity (CUPRAC), and total antioxidant capacities (TAC). The antimicrobial activity of ethyl acetate and butanolic extracts of G. microcephala in addition of fatty acids content of different parts of the studied plant was evaluated against human pathogenic bacteria, fish bacteria and two yeast. The used methods were agar diffusion method and the determination of minimal bactericidal and fungicidal concentrations (MBC and MFC). No significant difference was found between the two used solvents on total phenolic content (TPC). While, difference in total flavonoid content (TFC) were found to be significant. Ethylene acetate extract was the most actif regarding antioxidant assay. The tested activity seems to be due to the high content of this extract on isoflavonoids. The tested extracts demonstrated the lowest inhibition concentration for B.cereus RSKK 863.

scholarly journals Screening of Ten Tomato Varieties Processing Waste for Bioactive Components and Their Related Antioxidant and Antimicrobial Activities

Antioxidants ◽  
2019 ◽  
Vol 8 (8) ◽  
pp. 292 ◽  
Author(s):  
Katalin Szabo ◽  
Zorița Diaconeasa ◽  
Adriana-Florinela Cătoi ◽  
Dan Cristian Vodnar
Keyword(s):  
High Performance ◽  
Antimicrobial Effect ◽  
Antimicrobial Activities ◽  
Bioactive Molecules ◽  
Total Phenolic ◽  
Bacterial Strains ◽  
Total Carotenoids ◽  
Tomato Production ◽  
The Mean ◽  
Antioxidant And Antimicrobial Activities

Global tomato production is currently around 180 million tons, of which more than a quarter undergoes processing. The removed peels, seeds, and vascular tissues usually end up in landfills, creating environmental pollution. In order to highlight the alternative use of these vegetal wastes, our study investigated 10 tomato varieties in terms of carotenoids content, phenolic composition, and their related antioxidant and antimicrobial activities. Tomato peels extracts were screened by high performance liquid chromatography with diode-array detection (HPLC/DAD) for qualitative and quantitative analyses. The extracts were tested against six bacterial strains to determine their antimicrobial effect; the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay was applied to estimate their antioxidant capacity. Total carotenoids content was significantly higher in Ţărănești roz, a local variety (5.31 ± 0.12 mg/100 g DW), while Mirsini, a commercial hybrid, presented significantly higher total phenolic content (155 ± 2 mg/100 g DW) compared to the mean value of all analyzed samples. The methanolic extracts of tomato peels presented acceptable antimicrobial activity against Staphilococcus aureus and Bacillus subtilis, and the mean antioxidant activity was 201 ± 44 µmol Trolox/100 g DW tomato peels. Considering that tomato peels have lycopene, β-carotene, lutein, and different phenolic compounds in their composition, tomato industrial by-products could represent a source of natural bioactive molecules with applicability in nutraceuticals and food industry.

scholarly journals Evaluation of Phenolic Compounds and Antioxidant and Antimicrobial Activities of Some Common Herbs

2017 ◽  
Vol 2017 ◽  
pp. 1-6 ◽  
Author(s):  
Muhammad Abdul Qadir ◽  
Syeda Kiran Shahzadi ◽  
Asad Bashir ◽  
Adil Munir ◽  
Shabnam Shahzad
Keyword(s):  
Antimicrobial Activity ◽  
Linoleic Acid ◽  
Reducing Power ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Bacterial Strains ◽  
Fungal Strains ◽  
Antioxidant And Antimicrobial Activities

The study was designed to evaluate the phenolic, flavonoid contents and antioxidant and antimicrobial activities of onion (Allium cepa), garlic (Allium sativum), mint (Mentha spicata), thyme (Thymus vulgaris), oak (Quercus), aloe vera (Aloe barbadensis Miller), and ginger (Zingiber officinale). All extracts showed a wide range of total phenolic contents, that is, 4.96 to 98.37 mg/100 g gallic acid equivalents, and total flavonoid contents, that is, 0.41 to 17.64 mg/100 g catechin equivalents. Antioxidant activity (AA) was determined by measuring reducing power, inhibition of peroxidation using linoleic acid system, and 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) scavenging activity. Different extracts inhibited oxidation of linoleic acid by 16.6–84.2% while DPPH radical scavenging activity (IC50 values) ranged from 17.8% to 79.1 μg/mL. Reducing power at 10 mg/mL extract concentration ranged from 0.11 to 0.84 nm. Furthermore the extracts of these medicinal herbs in 80% methanol, 80% ethanol, 80% acetone, and 100% water were screened for antimicrobial activity by disc diffusion method against selected bacterial strains, Staphylococcus aureus, Escherichia coli, Bacillus subtilis, and Pasteurella multocida, and fungal strains, Aspergillus niger, Aspergillus flavus, Rhizopus solani, and Alternaria alternata. The extracts show better antimicrobial activity against bacterial strains as compared to fungal strains. Results of various assays were analyzed statistically by applying appropriate statistical methods.

scholarly journals Grape Canes from Typical Cultivars of Campania (Southern Italy) as a Source of High-Value Bioactive Compounds: Phenolic Profile, Antioxidant and Antimicrobial Activities

Molecules ◽  
2021 ◽  
Vol 26 (9) ◽  
pp. 2746
Author(s):  
Giuseppe Squillaci ◽  
Carla Zannella ◽  
Virginia Carbone ◽  
Paola Minasi ◽  
Veronica Folliero ◽  
...  
Keyword(s):  
Southern Italy ◽  
Radical Scavenging Activity ◽  
Antiviral Agents ◽  
Radical Scavenging ◽  
Antimicrobial Activities ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Herpes Simplex Viruses ◽  
Ferric Reducing Antioxidant Power ◽  
Antioxidant And Antimicrobial Activities

The purpose of the current study was to determine the phenolic composition, antioxidant, and antimicrobial activities in grape cane extracts from typical cultivars of Southern Italy. Aqueous extracts at different pHs (1–13) were prepared from “Aglianico”, “Fiano”, and “Greco” grape canes. The results demonstrated that an alkaline pH (13.00) produced the best polyphenol-rich extracts, as the total phenolic content was more than double when compared to the respective extracts prepared at pH 1.00. “Greco” grape canes gave the highest quantity of phenolic compounds at each pH, ranging from 42.7 ± 0.4 to 104.3 ± 3.0 mg Gallic Acid Equivalents (GAE)/g Dry Extract (DE) from pH 1.00 to 13.00. The Radical Scavenging Activity (RSA) and the Ferric Reducing Antioxidant Power (FRAP) were measured. The highest antioxidant activity was showed by “Greco” extract at pH 7.00. Seventy-five compounds were identified in the extracts by HPLC-MS with six of them described for the first time in grape canes. Procyanidins were highly abundant in extracts at pH 7.00, whereas stilbenoids were the most represented compounds at pH 13.00. Very strong antiviral activity against herpes simplex viruses was recorded for the extracts at pH 7.00 and 13.00 that were active in the early stages of infection by acting directly against the viral particles. The overall results suggest that grape canes, currently underutilized, can be usefully valorised by providing active extracts to use as antioxidant and antiviral agents.

scholarly journals Volatile Compounds and Antioxidant and Antimicrobial Activities of Selected Citrus Essential Oils Originated from Nepal

Molecules ◽  
2021 ◽  
Vol 26 (21) ◽  
pp. 6683
Author(s):  
Devi Prasad Bhandari ◽  
Darbin Kumar Poudel ◽  
Prabodh Satyal ◽  
Karan Khadayat ◽  
Sital Dhami ◽  
...  
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Citrus Sinensis ◽  
Antioxidant Properties ◽  
Antimicrobial Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Diffusion Method ◽  
Citrus Species ◽  
Citrus Peel ◽  
Antioxidant And Antimicrobial Activities

Citrus species of plants are among the most commercially cultivated crops, mainly for their fruit. Besides, the generally consumed flesh inside the fruit, the peel is quite important too. Essential oils extracted from the peel have a history of being used by humankind for centuries. These essential oils are rich in antioxidants and antimicrobial agents. Comparative investigation of volatile constituents, and antioxidant and antimicrobial activities were undertaken. The essential oils were evaluated through gas chromatography-mass spectrometry (GC–MS), and enantiomeric composition by chiral GC–MS. Similarly, the antioxidant properties were evaluated by 2,2-diphenyl-1-picrylhydrazyl scavenging assay, and antimicrobial activities were assayed using the disk diffusion method. The highest extraction yield of 1.83% was observed in Citrus sinensis Osbeck. GC–MS analysis showed limonene (63.76–89.15%), γ-terpinene (0.24–6.43%), β-pinene (0.15–6.09%), linalool (0.35–3.5%), sabinene (0.77–2.17%), myrcene (0.74–1.75%), α-terpineol (0.28–1.15%), and α-pinene (0.2–0.58%) as the major constituents of the essential oil of the Citrus species studied. For the first time, through our study, chiral terpenoids have been observed from Citrus grandis Osbeck essential oil. The order of antioxidant activity is as follows: Citrus grandis Osbeck red flesh > Citrus reticulata Blanco > Citrus sinensis Osbeck > Citrus grandis Osbeck white flesh. Except for Citrus grandis Osbeck white flesh (52.34 µL/mL), all samples demonstrated stronger antioxidant activities than those of the positive control, quercetin (5.60 µL/mL). Therefore, these essential oils can be used as a safe natural antioxidant to prevent product oxidation. Likewise, citrus peel essential oil showed antimicrobial activity against tested bacterial strains, albeit marginal.

scholarly journals Antifungal and Antibacterial Activities of Apple Vinegar of Different Cultivars

2021 ◽  
Vol 2021 ◽  
pp. 1-6
Author(s):  
Driss Ousaaid ◽  
Hassan Laaroussi ◽  
Meryem Bakour ◽  
Hayat Ennaji ◽  
Badiaa Lyoussi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Antimicrobial Effect ◽  
Antibacterial Activities ◽  
Antimicrobial Activities ◽  
Total Phenolic ◽  
Plate Method ◽  
Golden Delicious ◽  
Pathogenic Microbes

This study was designed to assess the antimicrobial potencies of apple vinegar against pathogenic microbes. The acidity and total phenolic content were carried out by titration with NaOH 0.1 N and the Folin–Ciocalteu method, respectively, while the spread plate method, agar well diffusion, and MIC assays were used to determine the antimicrobial activities of different vinegar samples. Acidity and phenolic content were dependent on the variety, where the highest values were observed in S2 with 4.02 ± 0.04% and 1.98 ± 0.05 mg GAE/mL for acidity and total phenolic content, respectively. The spread plate method revealed that samples S1 and S2 obtained from the Red delicious variety and Golden delicious variety, respectively, inhibit the growth of all tested strains, while S3 obtained from different varieties and S4 obtained from the Gala royal variety inhibit only two microbes (Escherichia coli and Vibrio cholerae). Sample S1 presented moderate antimicrobial effect against all examined strains with a diameter of inhibition ranging from 11 ± 0.7 to 19 ± 0.5 mm and with MIC values ranging between 1/2 and 1/100. The findings of the current study confirm the usefulness of apple vinegar as a natural sanitizer that inhibits the growth of pathogenic microbes.

Characterization of phenolic extracts from Brava extra virgin olive oils and their cytotoxic effects on MCF-7 breast cancer cells

2018 ◽  
Vol 119 ◽  
pp. 73-85 ◽  
Author(s):  
Patricia Reboredo-Rodríguez ◽  
Carmen González-Barreiro ◽  
Beatriz Cancho-Grande ◽  
Tamara Y. Forbes-Hernández ◽  
Massimiliano Gasparrini ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Cytotoxic Effects ◽  
Virgin Olive Oils ◽  
Olive Oils ◽  
Mcf 7 ◽  
Phenolic Extracts

scholarly journals Antioxidant and Antimicrobial Activities, and Phenolic Compounds of Selected Inula species from Turkey

2013 ◽  
Vol 8 (4) ◽  
pp. 1934578X1300800 ◽  
Author(s):  
Alper Gökbulut ◽  
Onural Özhana ◽  
Basri Satılmiş ◽  
Kadir Batçioğlu ◽  
Selami Günal ◽  
...  
Keyword(s):  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Antimicrobial Activities ◽  
Total Phenolic ◽  
Methanol Extracts ◽  
Plant Parts ◽  
Antimicrobial Potential ◽  
Phenolic Components ◽  
Antioxidant And Antimicrobial Activities

Three Inula species, I. viscosa, I. helenium ssp. turcoracemosa and I. montbretiana, collected from different locations of Anatolia were investigated for their antioxidant and antimicrobial potential, and their total phenolic content and phenolic composition. Antioxidant activities of various extracts of the plant parts were measured using DPPH radical scavenging and ABTS assays. Antimicrobial potential of methanol extracts of the plant parts was determined by the agar dilution method against Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa, Candida albicans and Candida tropicalis. All the extracts were more active against Gram-positive bacteria and yeasts than Gram-negative bacteria. The extracts exhibited antioxidant and antimicrobial activities in different concentrations. Total phenolic concentration of the extracts was estimated with Folin-Ciocalteu reagent using gallic acid as standard. The total phenolic content varied widely in different parts of the three tested Inula species, ranging from 21.1 ± 0.8 to 190.9 ± 6.1 mg GAE/g extract. Phenolic components, such as chlorogenic acid, caffeic acid, rutin, myricetin, quercetin, luteolin and kaempferol were quantified by HPLC-DAD in the methanol extracts of the Inula species. It was obvious that the antioxidant and antimicrobial properties of the plants were due to the phenolics.

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