scholarly journals Genotoxic Effects of Chlorpyrifos in Freshwater Fish Cirrhinus mrigala Using Micronucleus Assay

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Anita Bhatnagar ◽  
Abhay Singh Yadav ◽  
Navneet Cheema
Keyword(s):  
Surface Waters ◽  
Micronucleus Assay ◽  
Probit Analysis ◽  
Fresh Water Fish ◽  
Cirrhinus Mrigala ◽  
Water Fish ◽  
Treated Fish ◽  
Potential Tool ◽  
Different Doses

The genotoxicity of pesticides is an issue of worldwide concern and chlorpyrifos is one of the largest selling organophosphate agrochemicals that has been widely detected in surface waters of India. The studies on long term genotoxic biomarkers are limited; therefore, present study was carried out to analyze the incidence of nuclear anomalies in the blood cells of fresh water fish Cirrhinus mrigala using micronucleus (MN) assay as a potential tool for assessment of genotoxicity. Acute toxicity of chlorpyrifos was evaluated by exposing fingerlings to different doses of chlorpyrifos (1/20, 1/10, and 1/5 of LC50) and LC50 was calculated as 0.44 mg L−1 using probit analysis. Blood samples were taken on days 2, 4, 8, 12, 21, 28, and 35. In general, significant effects for both concentration and duration of exposure were observed in treated fish. It was found that MN induction was highest on day 14 at 0.08 mg L−1 concentration of chlorpyrifos. It was concluded that chlorpyrifos is genotoxic pesticide causing nuclear anomalies in Cirrhinus mrigala.

scholarly journals Effect of exposure to sublethal concentrations of zinc cyanide on tissue ATPase activity in the fresh water fish, Cirrhinus mrigala (Ham)

2012 ◽  
Vol 64 (1) ◽  
pp. 257-263 ◽  
Author(s):  
Shwetha Dube ◽  
B.B. Hosetti
Keyword(s):  
Adaptive Response ◽  
Aquatic Organisms ◽  
Fresh Water Fish ◽  
Cirrhinus Mrigala ◽  
Biomarkers Of Exposure ◽  
Maximum Inhibition ◽  
Water Fish ◽  
Treated Fish ◽  
Cyanide Intoxication ◽  
Sublethal Concentrations

The present study was conducted to evaluate the effects of zinc cyanide (Zn(CN)2) on the ATPase (Na+-K+-, Mg2+, Ca2+) activity of the freshwater fish Cirrhinus mrigala (Ham). Fish were exposed to two sublethal concentrations (0.114 and 0.068 mg/L) and the effects were studied in the liver, muscle and gill tissues at the end of 5, 10 and 15 days of exposure. Cyanide intoxication resulted in marked changes in ATPases, represented by a significant decrease in the activities. Maximum inhibition was observed at higher concentration. The order of inhibition in the activity was Na+-K+-> Ca2+ > Mg2+ ATPase. These results imply an immediate adaptive response to the stress, demonstrating alterations in ATPase activities in the tissues of cyanide-treated fish. The present study clearly proposes that ATPases can be used as biomarkers of exposure of aquatic organisms to cyanide intoxication.

scholarly journals Metabolic alterations in liver of fresh water fish, C. punctata exposed to arsenic: an adverse and adaptive response to the environment

2017 ◽  
Vol 6 (2) ◽  
pp. 87-94
Author(s):  
Md Shahidul Haque ◽  
Md Mahmudul Hasan ◽  
Md Maniruzzaman ◽  
Md Aktaruzzaman ◽  
Md Abu Zubair ◽  
...  
Keyword(s):  
Fresh Water ◽  
Triglyceride Level ◽  
Adaptive Response ◽  
Fresh Water Fish ◽  
Channa Punctata ◽  
Metabolic Alterations ◽  
Water Fish ◽  
Metabolic Activities ◽  
Different Doses

Arsenic is a major toxicant impairing in diverse metabolic alterations of the organisms and the mechanism of adaptive response is yet to be identified. In the present study, effects of different doses of arsenic in liver of Channa punctata on the regulation of metabolic activities were done. C. punctata, a variety of fresh water fish were exposed to 1, 10 and 1000 ?M concentration of Na2HAsO4 for 1 h. The amount of protein, in response to 1, 10 and 1000 ?M concentration of arsenic were augmented by 184.47% (2.84-folds), 202.82% (3.0- folds) and 317.49% (4.17-folds), respectively and was found to be higher for 1000 ?M dose. Cholesterol contents in liver were similarly exaggerated by 517.45% (6.17-folds), 308.13% (4.1-folds) and 286.41% (3.86-folds), respectively. However, the higher response was found for 1 ?M dose of Na2HAsO4. Similar stimulatory effects on triglyceride level were observed in response to arsenic. Na2HAsO4 causes 443.74% (5.43-folds), 533.11% (6.33-folds) and 548.48% (6.48-folds) enhanced triglyceride level in liver respectively and the effects were pronounced for 1000 ?M concentration. Our findings conclude that arsenic is involved in impairment of metabolic activities in liver of the species of fish and gives an impact to the environment for survival.Int. J. Agril. Res. Innov. & Tech. 6 (2): 87-94, December, 2016

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