scholarly journals Efficacy of EDTA and Phosphorous on Biomass Yield and Total Lipid Accumulation in Two Green Microalgae with Special Emphasis on Neutral Lipid Detection by Flow Cytometry

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Gour Gopal Satpati ◽  
Prakash Chandra Gorain ◽  
Ruma Pal
Keyword(s):  
Flow Cytometry ◽  
Neutral Lipid ◽  
Incubation Period ◽  
Total Lipid ◽  
Lipid Content ◽  
Total Lipid Content ◽  
Monounsaturated Fatty Acids ◽  
Maximum Biomass ◽  
Dry Cell Weight ◽  
Golden Yellow

Chlorella ellipsoidea and Chlorococcum infusionum, promising microalgae for biodiesel feedstock production, were treated with ethylenediaminetetraacetate (EDTA) and phosphorous to induce stress which was then followed by flow cytometry to study the enhanced intracellular neutral lipid content. Treatment resulted in up to a threefold increase in total lipid content of Chlorella (41.8±1.9% at 16 days of incubation period) and more than twofold increases in Chlorococcum (31.3±1.0% at 18 days of incubation period) under phosphorous starvation in the culture. It was observed that maximum biomass yields in Chlorella and Chlorococcum were 1.56±0.06 and 2.17±0.12 g/L at 1.5 g/L of phosphorous after 20 and 18 days of incubation periods, respectively. The qualitative analyses of neutral lipid bodies under stress conditions were performed by confocal microscopy and revealed bright golden-yellow lipid droplets in stress exposed cells. Significant increase of monounsaturated fatty acids under the nutrient limited conditions was suitable to produce biodiesel. The maximum biomass (g/L) and lipid content (% dry cell weight) at different stresses showed significant results (p<0.05) by single-factor Analysis of Variance (ANOVA) followed by Duncan’s Multiple Range Test (DMRT).

Stress of Nutrients Deficiency on Growth and Lipid Accumulation in Oil-Rich Navicula sp.

2014 ◽  
Vol 521 ◽  
pp. 68-71
Author(s):  
Xiang Ji ◽  
Jin Rong Wang ◽  
Xiao Li Miao ◽  
Kai Hua Liu ◽  
Lu Cai
Keyword(s):  
Citric Acid ◽  
Total Lipid ◽  
Lipid Content ◽  
Lipid Accumulation ◽  
Culture Medium ◽  
Nutrient Deficiency ◽  
Total Lipid Content ◽  
Maximum Biomass

Based on optimized aquatic diatom medium, treatment of nutrient deficiency was carried out to Navicula sp. and the analysis results of comprehensive biomass and total lipid content indicated that the optimal Tmass concentrations of TFeClB3B·3HB2BO,T TCdSOB4 Band Citric acid were 1.2×10P-5P mol/L, 0.8μg/L and 12 mg/L, respectively. Maximum biomass of Navicula sp. on this culture medium at 680 spectrophotometrically could reach 1.201. Final lipid content of this microalgae could be improved from 26.60% to 37.68% after the stress of nutrient deficiency treatment.

scholarly journals Biomass and lipid production of marine diatom Chaetoceros ChTA at different nutrient concentrations

2018 ◽  
Vol 15 (1) ◽  
pp. 113-120
Author(s):  
Nguyễn Thị Thu Liên ◽  
Nguyễn Hồng Sơn ◽  
Lê Thị Tuyết Nhân
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Total Lipid ◽  
Lipid Content ◽  
Sodium Nitrate ◽  
Sodium Phosphate ◽  
Total Lipid Content ◽  
Marine Diatom ◽  
Dry Cell Weight

Microalgae, especially diatom, are an optimal resource of polyunsaturated fatty acids (PUFAs). Recently, the new industrial scale culture systems are being developed for the purpose of producing the large amounts of biomass for biofuel, medicinal, cosmetical production. However, an economic utilization system needs to be standardized for the optimal production of the resources. In this way, manipulation of culture conditions (physicochemical factors) represents a solution to increase the lipid yield. The strain Chaetoceros ChTA is one of the high potential lipid strains, isolated from Thuan An coastal zone, Thua Thien Hue province. This paper deals with the growth and total lipid content of the marine diatom strain, Chaetoceros ChTA at different concentrations of sodium nitrate, sodium phosphate and sodium silicate under the laboratory condition. In addition, the fatty acid composition of the strain Chaetoceros ChTA growing in f/2 medium (control formula) was also analyzed. The result showed that this diatom strain successfully growed with maximum biomass production of 0.482g/L at the 112.50 mg/L of sodium nitrate meanwhile maximum total lipid content of 25.62% dry cell weight was recorded in the medium amended with 2.13 mg/L sodium phosphate. The fatty acid composition of this strain in f/2 medium (control) was analysed through GC-MS and the result showed that the saturated fatty acid content was 32.3% while the content of unsaturated fatty acid was 65.9% of the total fatty acid (TFA). The fatty acid composition also contains a significant amount of EPA (14.33%) and DHA is suitable for navigation use them as functional foods, pharmaceuticals and feed supplements for animals in aquaculture.

scholarly journals Effect of season on the fatty acid profile of total lipids, phospholipids and triacylglycerols in Mastacembelus mastacembelus (Atatürk Dam Lake, Turkey)

2018 ◽  
Vol 69 (1) ◽  
pp. 242 ◽  
Author(s):  
S. Kaçar ◽  
M. Başhan ◽  
S. A. Oymak
Keyword(s):  
Fatty Acids ◽  
Total Lipid ◽  
Lipid Content ◽  
Palmitoleic Acid ◽  
Saturated Fatty Acids ◽  
Total Lipid Content ◽  
Docosapentaenoic Acid ◽  
Monounsaturated Fatty Acids ◽  
Total Lipids ◽  
Atatürk Dam Lake

The present study investigated the lipid content and FA profiles of total lipids, phospholipids (PL) and triacylglycerols (TAG) of Mastacembelus mastacembelus from the Atatürk Dam Lake (Turkey). The results showed that the total lipid content of the dorsal muscle varied seasonally from 0.50% to 3.59%. Wide ranges of saturated fatty acids (SFAs) (36.21 to 50.52%) and polyunsaturated fatty acids (PUFAs) (25.22 to 42.02%) were found in the PL fraction. However, higher monounsaturated fatty acids (MUFAs) (37.63 to 45.07%) and SFA (34.71 to 38.82%) were found in TAG (triacylglycerol). The ratios of ω-3 to ω-6 PUFA ranged from 0.65 to 1.32 and 1.07 to 3.48 in PL (phospholipid) and TAG fraction, respectively. The results also showed that the major components were palmitic acid (C16:0), stearic acid (C18:0), oleic acid (C18:1ω-9), palmitoleic acid (C16:1ω-7), docosahexaenoic acid (DHA, C22:6ω-3), arachidonic acid (AA, C20:4ω-6) and docosapentaenoic acid (C22:5ω-3) in total lipid, C16:0, C18:1ω-9, AA and DHA in PL, C16:0, C16:1ω-7, C18:1ω-9, linoleic acid (LA, C18:2ω-6), and DHA in TAG extracted from the muscle of M. mastacembelus in all seasons.

scholarly journals Lipid and fatty acid profile of liver in relation to reproduction in the small-scaled terapon Terapon puta (Cuvier, 1829) from Pondicherry Coast, India

2018 ◽  
Vol 65 ◽  
Author(s):  
R. Nandikeswari ◽  
M. Sambasivam
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Fatty Acid Profile ◽  
Polyunsaturated Fatty Acid ◽  
Total Lipid ◽  
Lipid Content ◽  
Saturated Fatty Acids ◽  
Total Lipid Content ◽  
Dry Weight ◽  
Monounsaturated Fatty Acids

<p>The total lipid content as percentage of dry weight of liver of <em>Terapon puta</em> (Cuvier, 1829) varied at four different stages <em>viz.,</em> immature, maturing, mature and spent stages of reproduction. Highest levels of saturated fatty acids (SFA) was recorded in the liver of mature fishes (50.71%) followed by maturing (48.03%) and the lowest in immature fishes (38.91%). Highest levels of monounsaturated fatty acids (MUFA) were observed in the liver of maturing fishes (37.07%) followed by mature (34.09%) and the lowest levels in immature fishes (30.48%). Maturing fishes had higher liver polyunsaturated fatty acid  levels (PUFA) (13.54%), compared to immature (10.55%) and mature fishes (10.17%).</p>

Aspergillus flavusgrowth and aflatoxin production as influenced by total lipid content during growth and development of cottonseed

2017 ◽  
Vol 31 (1) ◽  
pp. 91-99 ◽  
Author(s):  
Kanniah Rajasekaran ◽  
Greg Ford ◽  
Kandan Sethumadhavan ◽  
Carol Carter-Wientjes ◽  
John Bland ◽  
...  
Keyword(s):  
Total Lipid ◽  
Lipid Content ◽  
Growth And Development ◽  
Total Lipid Content ◽  
Aflatoxin Production

Comparison of biomass production and total lipid content of freshwater green microalgae cultivated under various culture conditions

2013 ◽  
Vol 37 (2) ◽  
pp. 99-106 ◽  
Author(s):  
Geun Ho Gim ◽  
Jung Kon Kim ◽  
Hyeon Seok Kim ◽  
Mathur Nadarajan Kathiravan ◽  
Hetong Yang ◽  
...  
Keyword(s):  
Biomass Production ◽  
Total Lipid ◽  
Lipid Content ◽  
Total Lipid Content ◽  
Culture Conditions ◽  
Green Microalgae

scholarly journals Adipophilin and Perilipin 3 Correlate with Total Lipid Content in Human Breast Milk

2018 ◽  
Author(s):  
Tereza Pavlova ◽  
Zdenek Spacil ◽  
Veronika Vidova ◽  
Filip Zlamal ◽  
Eliska Cechova ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Breast Milk ◽  
Total Lipid ◽  
Lipid Content ◽  
Gravimetric Method ◽  
Total Lipid Content ◽  
Milk Lipid ◽  
Human Breast Milk ◽  
Human Breast

Objective: Lipids are secreted into milk as bilayer-coated structures: milk lipid globules (MLGs). Adipophilin (ADRP) and perilipin 3 (TIP47) are associated with MLGs in human breast milk; however, the role of these proteins in milk lipid secretion is not fully understood. The aim of the study was to investigate levels of ADRP, TIP47 and total lipid content in human breast milk, their mutual correlations and dynamics during lactation. Research Methods &amp; Procedures: Milk samples from 22 healthy lactating women (Caucasian, Central European) were collected at five time points during lactation (1&ndash;3, 12&ndash;14, 29&ndash;30, 88&ndash;90 and 178&ndash;180 days postpartum). Mass spectrometry-based method was used for quantification of ADRP and TIP47 in the samples. The gravimetric method was used to determine milk total lipid content. Results: We observed distinctive trends in ADRP, TIP47 levels and lipid content in human breast milk during the first 6 months of lactation. We also found a significant association between lipid content and ADRP, lipid content and TIP47, and ADRP and TIP47 concentrations in breast milk at all sampling points. Moreover, we derived an equation for estimating the mean lipid content of milk based on ADRP concentration in human breast milk. Conclusions: A mass spectrometry-based method was developed for quantifying ADRP and TIP47 in human breast milk. Strong mutual correlations were found between ADRP, TIP47 and total lipid content in human breast milk.

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