scholarly journals Simultaneous Determination of Four Active Ingredients inSargentodoxa cuneataby HPLC Coupled with Evaporative Light Scattering Detection

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Di-Hua Li ◽  
Yuan-Shan Lv ◽  
Jun-Hong Liu ◽  
Lei Yang ◽  
Yan Wang ◽  
...  
Keyword(s):  
Light Scattering ◽  
Simultaneous Determination ◽  
Flow Rate ◽  
Evaporative Light Scattering Detection ◽  
Light Scattering Detection ◽  
Evaporative Light Scattering ◽  
Relative Standard ◽  
Acid Aqueous Solution ◽  
Interday Precision

A HPLC coupled with evaporative light scattering detection method had been developed for the simultaneous determination of 3,4-dihydroxyphenylethyl alcohol glycoside, salidroside, chlorogenic acid, and liriodendrin in the stem ofSargentodoxa cuneata. With a C18 column, the analysis was performed using acetonitrile and 0.2% formic acid aqueous solution as mobile phase in gradient program at a flow rate of 0.9 mL/min. The optimum drift tube temperature of evaporative light scattering detection was at 105°C with the air flow rate of 2.5 L/min. The calibration curves showed good linearity during the test ranges. This method was validated for limits of detection and quantification, precision, and reproducibility. The recoveries were within the range of 96.39%–104.64%. The relative standard deviations of intraday and interday precision were less than 2.90% and 3.30%, respectively. The developed method can be successfully used to quantify the four analytes in the stem ofSargentodoxa cuneatafrom various regions in China.

scholarly journals Determination of Nine Intense Sweeteners in Foodstuffs by High-Performance Liquid Chromatography and Evaporative Light-Scattering Detection: Interlaboratory Study

2009 ◽  
Vol 92 (1) ◽  
pp. 208-222 ◽  
Author(s):  
Manuela Buchgraber ◽  
Andrzej Wasik ◽  
S Casal ◽  
A Dubois ◽  
B Gutsche ◽  
...  
Keyword(s):  
Light Scattering ◽  
High Performance ◽  
High Performance Liquid Chromatographic ◽  
Evaporative Light Scattering Detection ◽  
Limit Of Quantification ◽  
Recovery Rates ◽  
Light Scattering Detection ◽  
Evaporative Light Scattering ◽  
Relative Standard

Abstract An interlaboratory trial was conducted to validate an analytical method based on high-performance liquid chromatographic analysis with evaporative light-scattering detection for the simultaneous determination of 9 intense sweeteners, i.e., acesulfame-K, alitame, aspartame, cyclamic acid, dulcin, neotame, neohesperidine dihydrochalcone, saccharin, and sucralose in carbonated and noncarbonated soft drinks and canned or bottled fruits. Seven laboratories participated in the validation study. The majority of the samples fortified with levels close to the limit of quantification had relative standard deviation for reproducibility (RSDR) values <15. In most cases, the recovery rates ranged between 90 and 105, demonstrating satisfactory performance of the method. For samples fortified at levels comparable to the prescribed legal limits stipulated in the current European Union legislation, the method produces acceptably accurate, repeatable, and reproducible results. Trueness, expressed in terms of recovery rates, was demonstrated in most cases by values ranging from 90 to 108. Comparability of results obtained by individual testing laboratories was good (RSDR values <10) for the majority of results. Moreover, HorRat values of <1.1 suggested good performance of the method for all sweeteners and matrixes tested.

Fingerprint Analysis of Flos Lonicerae by High-Performance Liquid Chromatography with Evaporative Light Scattering Detection

2012 ◽  
Vol 554-556 ◽  
pp. 2058-2063
Author(s):  
Feng Lai Lu ◽  
Chuan Ming Fu ◽  
Hai Ying Jiang ◽  
Yue Yuan Chen ◽  
Jin Lei Liu ◽  
...  
Keyword(s):  
Light Scattering ◽  
Flow Rate ◽  
Retention Time ◽  
High Performance ◽  
Evaporative Light Scattering Detection ◽  
Fingerprint Analysis ◽  
Light Scattering Detection ◽  
Flos Lonicerae ◽  
Evaporative Light Scattering ◽  
Relative Standard

A high-performance liquid chromatographic method (HPLC) with evaporative light scattering detection (ELSD) has been developed for fingerprint analysis of Flos Lonicerae. The samples were separated with an Agilent C18 column using acetonitrile (A) and 0.1% formic acid solution under gradient conditions (0→10 min：11.5%→15% A, 10→12 min：15% →22% A, 12→18min：22%→33% A; 18→30min: 33%→45% A) as the mobile phase at a flow rate of 0.8ml min−1 within 30 min. The ELSD conditions were optimized at nebulizer-gas flow rate 2.7 L min−1 and drift tube temperature 105°C. Precision experiments showed relative standard deviation (R.S.D.) of peak area and retention time were better than 2.5%, inter-day and intra-day variabilities showed that R.S.D. was ranged from 0.66% to 4.17%. Accuracy validation showed that average recovery was between 95.33% and 104.10%. The method was validated to achieve the satisfactory precision and recovery. Relative retention time and relative peak area were used to identify the common peaks for fingerprint analysis. There are nine common peaks in the fingerprint. The quality of sixteen batches of Flos Lonicerae samples was evaluated by hierarchical clustering analysis, which classfied all bacthes into two clusters. Furthermore, the contents of important medicinal compounds (chlorogenic acid, macranthoidin B and dipsacoside B) in different batches of Flos Lonicerae samples were determined simultaneously using the developed HPLC-ELSD method. The developed analytical procedure was proved to be a reliable and rapid method for the quality control of Flos Lonicerae.

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