scholarly journals The Essential Oils of Rhaponticum carthamoides Hairy Roots and Roots of Soil-Grown Plants: Chemical Composition and Antimicrobial, Anti-Inflammatory, and Antioxidant Activities

2016 ◽  
Vol 2016 ◽  
pp. 1-10 ◽  
Author(s):  
Ewa Skała ◽  
Patrícia Rijo ◽  
Catarina Garcia ◽  
Przemysław Sitarek ◽  
Danuta Kalemba ◽  
...  

The essential oils were isolated by hydrodistillation from the hairy roots (HR) and roots of soil-grown plants (SGR) of Rhaponticum carthamoides and were analyzed by GC-MS method. In the both essential oils 62 compounds were identified. The root essential oils showed the differences in the qualitative and quantitative composition. The sesquiterpene hydrocarbons (55–62%) dominated in both essential oils. The major compounds of HR essential oil were cyperene, 13-norcypera-1(5),11(12)-diene, and cadalene while aplotaxene, nardosina-1(10),11-diene, and dauca-4(11),8-diene dominated in SGR essential oil. Both essential oils showed antibacterial activity especially against Enterococcus faecalis (ATCC 29212) and Pseudomonas aeruginosa (ATCC 27853) (MIC value = 125 µg/mL). HR and SGR essential oils also decreased the expression of IL-1β, IL-6, and TNF-α and the ROS level in LPS-treatment astrocytes. This is the first report to describe the chemical composition of R. carthamoides essential oil from hairy roots, its protective effect against LPS-induced inflammation and ROS production in astrocytes, and its antimicrobial potential. The results show that R. carthamoides hairy roots may be a valuable source of the essential oil and may be an alternative to the roots of soil-grown plants.

2015 ◽  
Vol 10 (8) ◽  
pp. 1934578X1501000 ◽  
Author(s):  
Jelena G. Stamenković ◽  
Gordana S. Stojanović ◽  
Ivana R. Radojković ◽  
Goran M. Petrović ◽  
Bojan K Zlatković

The present study reports the chemical composition on the essential oil obtained from fresh roots, stems, inflorescences and fruits of Chaerophyllum temulum. In all samples, except the roots, the most dominant components were sesquiterpene hydrocarbons. ( Z)-Falcarinol was the principal constituent of the root essential oils (61.7% at the flowering stage and 62.3% at the fruiting stage). The blossom oil was dominated by ( Z,E)-α-farnesene (23.4%), ( E)-β-farnesene (9.0%) and germacrene D-4-ol (9%), whereas the oil from the fruit had germacrene D-4-ol (27.6%) as its main compound, accompanied by ( Z,E)-α-farnesene (13.4%). Germacrene D was the most abundant component of the stem essential oil (38.4% at the flowering stage and 32.5% at the fruiting stage). The obtained results show that the qualitative composition of the oil depends on the part of the plant which is analyzed, while the quantitative composition of the main components depends on the growing stage of the plant.


2018 ◽  
Vol 2 (6) ◽  
pp. 410 ◽  
Author(s):  
Danielle C. S. Oliveira ◽  
Telma Mary Kaneko ◽  
Maria Cláudia Marx Young ◽  
Cynthia Murakami ◽  
Inês Cordeiro ◽  
...  

Eugenia dysenterica DC is a native species from the Cerrado biome and can be found in several states of Brazil. This study evaluated the chemical composition, antimicrobial and antioxidant activities from the essential oils of a population collected in São Paulo state. Essential oils were obtained by conventional means, and their compositions were analyzed by GC-MS. Screening assays for antimicrobial activity were carried out by the microdilution method and the antioxidant potential was assessed by the DPPH scavenging method. The GC-MS analysis indicated that 52.63% of the essential oil is composed by oxygenated sesquiterpenes and the major compound is (-)-elema-1,3,11(13)-trien-12-ol (24.86%). The antimicrobial assay indicated MIC 42.1 µg/mL for S. aureus and MIC > 10000 µg/mL for the other tested microorganisms, Gram negative bacteria and fungi. The oil showed an IC50 of 5.4±0.632 mg/mL for in the DPPH assay. The essential oil had a different chemical composition from previous studies. The essential oil did not present a potent antioxidant activity. However, it can be considered a promising antimicrobial agent against S. aureus.


2019 ◽  
Vol 2019 ◽  
pp. 1-9 ◽  
Author(s):  
Joseph Nana Gyesi ◽  
Rita Opoku ◽  
Lawrence Sheringham Borquaye

Annona muricata, also called soursop, is widespread in many tropical countries, and various parts of the plant have been shown to possess very good pharmacological properties. This work evaluated the chemical composition and antioxidant activities of essential oils obtained from the fruit pulp and leaves of soursop. Essential oils were obtained via hydrodistillation and characterized by gas chromatography-mass spectrometry. Antioxidant potential was evaluated via the phosphomolybdenum, hydrogen peroxide scavenging, and 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assays. In the leaf essential oil, a total of 31 compounds were identified with δ-cadinene (22.58%) and α-muurolene (10.64%) being the most abundant. Thirty-two compounds were identified in the fruit pulp essential oil with Ç-sitosterol (19.82%) and 2-hydroxy-1-(hydroxymethyl) ethyl ester (13.48%) being present in high amounts. Both essential oils showed very good total antioxidant capacities (49.03 gAAE/100 g and 50.88 gAAE/100 g for fruit pulp and leaf essential oils, respectively). The IC50 values from the DPPH assay were 244.8 ± 3.2 μg/mL for leaf essential oil and 512 ± 5.1 μg/mL for the fruit pulp essential oil. At 1 mg/mL, hydrogen peroxide scavenged was below 50% for both leaf and fruit pulp essential oils, indicating moderate activity. These results suggest possible application of the essential oils of Annona muricata in food preservation and processing.


Molecules ◽  
2021 ◽  
Vol 26 (3) ◽  
pp. 643
Author(s):  
Giorgia Catinella ◽  
Natale Badalamenti ◽  
Vincenzo Ilardi ◽  
Sergio Rosselli ◽  
Laura De Martino ◽  
...  

The chemical composition and the qualitative and quantitative variability of the essential oils of three taxa belonging to the Teucrium genus were studied. The investigated taxa, that grow wild in Sicily, were Teucrium flavum L. (section Chamaedrys (Mill.) Scheb.), Teucrium montanum and Teucrium capitatum L. of section Polium (Mill.) Scheb. Essential oils were extracted by hydrodistillation and analyzed by GC-MS. In total, 74 compounds were identified. Sesquiterpene hydrocarbons were found to be the main group for T. flavum (48.3%). T. capitatum consisted essentially of monoterpene hydrocarbons (72.7%), with α-pinene (19.9%), β-pinene (27.6%) and sylvestrene (16.6%) as the most abundant compounds whereas ledene oxide (12.1%), epiglobulol (13.5%) and longifolenaldehyde (14.5%) were identified as the main constituents among the oxygenated sesquiterpenes (63.5%) of T. montanum. Furthermore, a complete literature review on the composition of the essential oils of all the other accessions of these Teucrium taxa, studied so far, was performed. Hierarchical Cluster Analysis (HCA) and Principal Component Analyses (PCA) were used in order to demonstrate geographical variations in the composition of the essential oils.


2020 ◽  
pp. 24-28
Author(s):  
Ravi Subban ◽  
Sundaram Kaveri

Artabotrys species which belongs to Annonaceae family are pleasant smelling and it is attributed to the presence of mono and sesquiterpenoids present in the essential oil of the plant. The objective of the present work is to review the chemical composition of the essential oils reported from twenty different Artabotrys species from various parts of the world. In the various Artabotrys species, the major compounds are monoterpene and sesquiterpene hydrocarbons and oxygenated sesquiterpenes.  The frequently and most commonly identified constituents are β-caryophyllene, caryophyllene oxide, 3-Carene, cyperene, cyperenone and 1,5-epoxy-salvial4(14)-ene.  Other constituents seems to be more specific to the respective Artabotrys species.


2009 ◽  
Vol 4 (6) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
Ana P. Murray ◽  
María S. Vela Gurovic ◽  
Silvana A. Rodriguez ◽  
María G. Murray ◽  
Adriana A. Ferrero

The essential oils of Schinus areira L. and S. longifolia (Lindl.) Speg. (Anacardiaceae) have been studied for their in vitro anti-acetylcholinesterase and antioxidant activities. The chemical composition of the oils obtained by hydrodistillation was determined by GC-MS. Fruit and leaf oils of S. areira were analyzed separately. The essential oil from S. longifolia elicited marked enzymatic inhibition (IC50 = 20.0 ± 1.0 μg/mL) and showed radical scavenger activity (IC50 = 25.2 ± 2.4 μg/mL). The essential oil from S. areira leaves was more active than that of the fruits in both bioassays.


2018 ◽  
Vol 18 (3-4) ◽  
pp. 195-203
Author(s):  
Y.N. Otaifah ◽  
A. Bouyahya ◽  
A. Talbaoui ◽  
H. Harhar ◽  
S. El Hajjaji

Medicinal plants have been used since time immemorial to fight against diseases. The biological activities of these plants may be due to the presence of bioactive compounds such as essential oils. The aim of this study was to determine the chemical composition of three Yemen medicinal plants, and e evaluate their antibacterial and antioxidant activities. The chemical composition of essential oils was determined using gas chromatography–mass spectrometry (GC–MS) analysis. The antibacterial effect was evaluated against pathogenic bacterial strains using the micro-platoon method and the antioxidant activity was estimated by DPPH (2,2-diphenyl-1-picrylhydrazyl) scanning radical assay. Essential oils (EOs) are rich in phenolic compound. Carvotanacetone is the main compound of Pulicaria jaubertii (PJ) essential oil (82.35%) and β-maaliene (64.94%) is the major compound of Plectranthus montanus Benth (PMB) essential oil, while, Tarchonanthus camphoratus (TC) essential oil possesses several main compounds such as fenchol (14.5%), α-pinene (12.43%), and α-terpineol (8.58%). Essential oils of PJ and PMB showed remarkable antibacterial effect against Staphylococcus aureus (minimum inhibitory concentration, MIC = 0.25% [v/v]), and PJEO against Escherichia coli (MIC = 0.25%[v/v]); the lowestMBC (minimum bactericidal concentration) values was obtained with PJEO against Staphylococcus aureus (CMI = 0.25% [v/v]). TCEO interestingly showed capacity while scanning the DPPH radical (6.32 mg E Trolox). These findings showed the biological potential of Yemeni aromatic medicinal plants as antibacterial and antioxidant agents. However, further investigations are needed to determine the antibacterial and antioxidant mechanisms of these oils.


Author(s):  
Dalva Paulus ◽  
Luana Aline Luchesi ◽  
Cleverson Busso ◽  
Marcela Tostes Frata ◽  
Paula Juliane Barbosa de Oliveira

Aims: The biological properties of essential oils represent possible therapeutic alternatives, with antimicrobial and antioxidant activities, and application in many areas of the industry. The objective was to determine the yield, chemical composition, antibacterial and antioxidant activities of the essential oils of Lavandula angustifolia, Pogostemon cablin, Rosmarinus officinalis, and Thymus vulgaris against Staphylococcus aureus, Salmonella enteritidis, Escherichia coli and Pseudomonas aeruginosa. Place and Duration of Study: The experiment was conducted at the microbiology laboratory of the Federal University of Technology - Paraná, Brazil, in the period between June 2016 to May 2017. Methodology: The essential oils were analyzed by gas chromatography coupled to mass spectrometry. The antibacterial activity was determined by microdilution in broth, showing minimum inhibitory concentration and minimum bactericidal concentration. The antioxidant activity was evaluated by scavenging of 2,2-diphenyl-1-picryl hydrazyl radical (DPPH). Results: The average yields of essential oils from L. angustifolia, P. cablin, R. officinalis,and  T. vulgaris were (%) 0.85; 2.0; 1.20, and 1.19, respectively. The major components of lavender essential oil were linalyl acetate (40.1%) and linalool (35.2%); for P. cablin - patchoulol (31.5%), seichelene (13.6%) and α-bulnesene (15.6%); for rosemary - camphor (32.5%), 1.8-cineole (13.6%) and α-pinene (9.8); for T. vulgaris - thymol (47%), o-scimene (21.6%), and carvacrol (11.4%). Thyme oil showed the best results for antibacterial activity, and low values (0.195 µL mL-1) of minimum inhibitory concentration were needed to inhibit S. aureus and S. enteritidis, and for L. angustifolia, P. cablin, and R. officinalis higher concentrations of essential oil were required. The essential oils of P. cablin and T. vulgaris had the strongest antioxidant properties (12.08 and 10.2 µmol trolox mL−1). Conclusion: The essential oils evaluated have an inhibitory effect on the microorganisms under study and also interesting antioxidant activity, which could be used by medicine to control bacterial infections, with potential application as natural food preservatives and as nutraceuticals.


2016 ◽  
Vol 46 (5) ◽  
pp. 937-942 ◽  
Author(s):  
Marcelo Nogueira Xavier ◽  
Cássia Cristina Fernandes Alves ◽  
Cristiane de Melo Cazal ◽  
Nathalia Horrana Santos

ABSTRACT: The Annonaceae family consists of ~135 genera with diverse species and a large number of chemical compounds arising from the secondary metabolism. However, the chemical composition of the essential oil of several species of the family such as Cardiopetalum calophyllum has not been completely determined. In this study, the essential oils extracted from the leaves, flowers, and fruits of C. calophyllum , collected in typical areas of the Cerrado of Goiás, were characterized. The essential oil was extracted by hydrodistillation for 4h using a Clevenger apparatus. The chemical analysis was carried out by gas chromatography-mass spectrometry (GC-MS), and the constituents were identified by comparing the spectra and retention index obtained with those available in the literature. Twenty three compounds were identified in the essential oil of leaves; the oxygenated sesquiterpene, spathulenol (28.78%), was the major compound. Twenty five compounds were identified in the flowers, mainly comprising sesquiterpene hydrocarbons, germacrene-D (37.03%) and germacrene-B (13.72%). Seventeen compounds were reported in fruits, mainly comprising sesquiterpene hydrocarbons, germacrene-D (28.19%) and germacrene-B (20.90%), and an oxygenated sesquiterpene, spathulenol (11.53%). This is the first report on the chemical composition of the essential oils from leaves, flowers, and fruits of C. calophyllum.


2018 ◽  
Vol 16 (S1) ◽  
pp. S155-S163 ◽  
Author(s):  
S. Mehalaine ◽  
O. Belfadel ◽  
T. Menasria ◽  
A. Messaili

The present study was carried out to determine, for the first time, the chemical composition and antibacterial activity of essential oils derived from the aerial parts of three aromatic plants Thymus algeriensis Boiss & Reut, Rosmarinus officinalis L., and Salvia officinalis L. growing under semiarid conditions. The essential oils were chemically analyzed and identified by gas chromatography (GC) and GC/ mass spectrometry (GC/MS) and their antimicrobial activity was individually evaluated against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa using both agar disk diffusion and agar dilution methods. The major constituents of Thymus algeriensis essential oil were identified as camphor (13.62%), 1,8-cineol (6.00%), borneol (5.74%), viridiflorol (4.00%), and linalool (3.93%). For Rosmarinus officinalis essential oil, 48 compounds were characterized, of which the main constituents were camphor (17.09%), Z-β-ocimene (10.88%), isoborneol (9.68%), α-bisabolol (7.89%), and borneol (5.11%). While, Salvia officinalis essential oil was characterized by β-thujone (16.44%), followed by viridiflorol (10.93%), camphor (8.99%), 1,8-cineol (8.11%), trans-caryophyllene (5.85%), and α-humulene (4.69%) as the major components. Notably, results from antibacterial screening indicated that Thymus algeriensis and Salvia officinalis essential oils exhibited a strong inhibitory effect against both Escherichia coli and Staphylococcus aureus compared to Rosmarinus officinalis essential oil. Further, less activity was recorded against Pseudomonas aeruginosa for the three tested essential oils.


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