scholarly journals Procaine Induces Epigenetic Changes in HCT116 Colon Cancer Cells

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Hussein Sabit ◽  
Mariam B. Samy ◽  
Osama A. M. Said ◽  
Mokhtar M. El-Zawahri
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Cell Line ◽  
Dna Fragmentation ◽  
Cancer Cell Line ◽  
Colon Cancer Cell Line ◽  
Drug Combinations ◽  
Colon Cancer Cell ◽  
Colon Cancer Cells ◽  
The World

Colon cancer is the third most commonly diagnosed cancer in the world, and it is the major cause of morbidity and mortality throughout the world. The present study aimed at treating colon cancer cell line (HCT116) with different chemotherapeutic drug/drug combinations (procaine, vorinostat “SAHA,” sodium phenylbutyrate, erlotinib, and carboplatin). Two different final concentrations were applied: 3 μM and 5 μM. Trypan blue test was performed to assess the viability of the cell before and after being treated with the drugs. The data obtained showed that there was a significant decrease in the viability of cells after applying the chemotherapeutic drugs/drug combinations. Also, DNA fragmentation assay was carried out to study the effect of these drugs on the activation of apoptosis-mediated DNA degradation process. The results indicated that all the drugs/drug combinations had a severe effect on inducing DNA fragmentation. Global DNA methylation quantification was performed to identify the role of these drugs individually or in combination in hypo- or hypermethylating the CpG dinucleotide all over the genome of the HCT116 colon cancer cell line. Data obtained indicated that different combinations had different effects in reducing or increasing the level of methylation, which might indicate the effectiveness of combining drugs in treating colon cancer cells.

scholarly journals Down-regulation of miR-543 expression increases the sensitivity of colorectal cancer cells to 5-Fluorouracil through the PTEN/PI3K/AKT pathway

2019 ◽  
Vol 39 (3) ◽  
Author(s):  
Gang Liu ◽  
JianPing Zhou ◽  
Ming Dong
Keyword(s):  
Colorectal Cancer ◽  
Colon Cancer ◽  
Cancer Cells ◽  
Cell Line ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Colon Cancer Cell Line ◽  
Colon Cancer Cell ◽  
Akt Pathway ◽  
Down Regulation

Abstract Resistance to chemotherapy is one of main obstacles in the treatment of colorectal cancer (CRC). However, the mechanisms are still unclear, and the treatment options are still limited. miR-543 has been indicated to act as an oncogene in some cancers, but its function in regulating chemoresistance has not been considered in CRC cells. This study investigated whether the down-regulation of miR-543 expression enhanced 5-fluorouracil (5-FU)-induced apoptosis in HCT8/FU colon cancer cells. In our study, qRT-PCR revealed that miR-543 expression was up-regulated in the HCT8/FU colon cancer cell line compared with that of HCT8 colon cancer cell line. An miR-543 inhibitor or mimic was transfected, followed by MTT assay to detect 5-FU sensitivity in HCT8 and HCT8/FU cell lines, which showed that IC50 of 5-FU was positively correlated with miR-543 expression. Further studies showed that miR-543 enhanced drug resistance by down-regulating the expression of phosphatase and tensin homolog (PTEN), which negatively regulates protein kinase B (AKT) activation. Additionally, an elevated expression of PTEN reversed the chemoresistance of miR-543-overexpressing HCT8 cells to 5-FU. These results indicate that miR-543 might be a target to increase the sensitivity of CRC cells to 5-FU through the PTEN/PI3K/AKT pathway.

Anticancer effect of X-Ray triggered methotrexate conjugated albumin coated bismuth sulfide nanoparticles on SW480 colon cancer cell line

2020 ◽  
Vol 582 ◽  
pp. 119320
Author(s):  
Mohammad Hasan Faghfoori ◽  
Hamed Nosrati ◽  
Hamed Rezaeejam ◽  
Jalil Charmi ◽  
Saeed Kaboli ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Colon Cancer Cell Line ◽  
Colon Cancer Cell ◽  
Bismuth Sulfide ◽  
Anticancer Effect ◽  
X Ray

scholarly journals Target disruption of the mutant β-catenin gene in colon cancer cell line HCT116: preservation of its malignant phenotype

Oncogene ◽  
2002 ◽  
Vol 21 (38) ◽  
pp. 5906-5911 ◽  
Author(s):  
Shigeki Sekine ◽  
Tatsuhiro Shibata ◽  
Michiie Sakamoto ◽  
Setsuo Hirohashi
Keyword(s):  
Colon Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Colon Cancer Cell Line ◽  
Colon Cancer Cell ◽  
Malignant Phenotype

scholarly journals In situ Proteomic Profiling of Curcumin Targets in HCT116 Colon Cancer Cell Line

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Jigang Wang ◽  
Jianbin Zhang ◽  
Chong-Jing Zhang ◽  
Yin Kwan Wong ◽  
Teck Kwang Lim ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Colon Cancer Cell Line ◽  
Colon Cancer Cell ◽  
Proteomic Profiling ◽  
Hct116 Colon Cancer Cell

scholarly journals A new method for detection of tumor driver-dependent changes of protein sialylation in a colon cancer cell line reveals nectin-3 as TGFBR2 target

2015 ◽  
Vol 24 (10) ◽  
pp. 1686-1694 ◽  
Author(s):  
Jennifer Lee ◽  
Uwe Warnken ◽  
Martina Schnölzer ◽  
Johannes Gebert ◽  
Jürgen Kopitz
Keyword(s):  
Colon Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Colon Cancer Cell Line ◽  
Colon Cancer Cell ◽  
New Method
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