scholarly journals Cardioprotective Effects of Astragalin against Myocardial Ischemia/Reperfusion Injury in Isolated Rat Heart

2016 ◽  
Vol 2016 ◽  
pp. 1-11 ◽  
Author(s):  
Daoxu Qu ◽  
Jichun Han ◽  
Huanhuan Ren ◽  
Wenxiao Yang ◽  
Xinjie Zhang ◽  
...  
Keyword(s):  
Myocardial Ischemia ◽  
Rat Heart ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Isolated Rat Heart ◽  
Sprague Dawley ◽  
Sod Activity ◽  
Cardioprotective Effects ◽  
Myocardial Ischemia Reperfusion ◽  
Isolated Rat

This study aims to evaluate the cardioprotective effects of astragalin against myocardial ischemia/reperfusion (I/R) injury in isolated rat heart. The cardioprotective effects of astragalin on myocardial I/R injury were investigated on Langendorff apparatus. Adult male Sprague-Dawley rats were randomly divided into five groups. The results showed that astragalin pretreatment improved myocardial function. Compared with I/R group, lactate dehydrogenase (LDH) and creatine kinase (CK) activities in coronary flow decreased in astragalin pretreatment groups, whereas superoxide dismutase (SOD) activity and glutathione/glutathione disulfide (GSH/GSSG) ratio significantly increased. The levels of malondialdehyde (MDA), intracellular reactive oxygen species (ROS), tumor necrosis factor-α(TNF-α), and interleukin-6 (IL-6) decreased in astragalin-treated groups. The infarct size (IS) and apoptosis rate in hearts from astragalin-treated groups were lower than those in hearts from the I/R group. Western blot analysis also revealed that astragalin preconditioning significantly reduced Bax level, whereas Bcl-2 was increased in the myocardium. Therefore, astragalin exhibited cardioprotective effects via its antioxidative, antiapoptotic, and anti-inflammatory activities.

scholarly journals Protective Effects ofElaeagnus angustifoliaLeaf Extract against Myocardial Ischemia/Reperfusion Injury in Isolated Rat Heart

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Binsheng Wang ◽  
Hengyi Qu ◽  
Jun Ma ◽  
Xiling Sun ◽  
Dong Wang ◽  
...  
Keyword(s):  
Myocardial Ischemia ◽  
Aqueous Extract ◽  
Rat Heart ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Isolated Rat Heart ◽  
Protective Effects ◽  
Elaeagnus Angustifolia ◽  
Myocardial Ischemia Reperfusion ◽  
Isolated Rat

The purpose of this study is to clarify the cardioprotective property of the aqueous extract ofElaeagnus angustifoliaL. leaf (EA) against myocardial ischemia/reperfusion injury in isolated rat heart. The myocardial ischemia/reperfusion (I/R) injury model of isolated rat heart was set up by the use of improved Langendorff retrograde perfusion technology. Compared with the ischemia/reperfusion (I/R) group, the aqueous extract ofElaeagnus angustifoliaL. leaf (0.5 mg/mL, 1.0 mg/mL) pretreatment markedly improved the coronary flow (CF) and raised left ventricular developed pressure (LVDP) and maximum rise/down velocity (±dp/dtmax). The infarct size of the EA-treated hearts was smaller than that of I/R group. After treatment with EA, the superoxide dismutase (SOD) activity increased; malondialdehyde (MDA) and protein carbonyl content reduced more obviously (P<0.01) than that of I/R injury myocardial tissue.Conclusion. Results from the present study showed that the aqueous extract ofElaeagnus angustifoliaL. leaf has obvious protective effects on myocardial I/R injury, which may be related to the improvement of myocardial oxidative stress states.

Protective Effect of Apigenin on Ischemia/Reperfusion Injury of the Isolated Rat Heart

2014 ◽  
Vol 15 (3) ◽  
pp. 241-249 ◽  
Author(s):  
Jing Hu ◽  
Zilin Li ◽  
Li-ting Xu ◽  
Ai-jun Sun ◽  
Xiao-yan Fu ◽  
...  
Keyword(s):  
Protective Effect ◽  
Reperfusion Injury ◽  
Rat Heart ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Isolated Rat Heart ◽  
Isolated Rat
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