scholarly journals Antioxidant and Antihyperlipidemic Effects ofCampomanesia adamantiumO. Berg Root

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Priscilla Pereira de Toledo Espindola ◽  
Paola dos Santos da Rocha ◽  
Carlos Alexandre Carollo ◽  
Wanderlei Onofre Schmitz ◽  
Zefa Valdivina Pereira ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Body Weight ◽  
Serum Level ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
The Body ◽  
Hepatic Enzymes ◽  
Liver Mass ◽  
Cardiometabolic Diseases ◽  
Organ Mass

Campomanesia adamantiumO. Berg, popularly known as guavira, has been used in Brazilian traditional medicine for reduction of serum lipid. The present study was carried out to investigate the antioxidant and antihyperlipidemic effects ofCampomanesia adamantiumroot aqueous extract (ExCA). Phenolic compounds were quantified in the ExCA and gallic and ellagic acids were identified by HPLC. ExCA showed efficiency in 2,2-diphenyl-1-picrylhydrazyl free radical scavenging, with IC50similar to butylhydroxytoluene control, and protected the erythrocytes against lipid peroxidation induced by 2,2′-azobis(2-methylpropionamidine) dihydrochloride, reducing generated malondialdehyde. Hyperlipidemic Wistar rats treated daily by gavage during eight weeks with ExCA (200 mg/kg of body weight) showed reduced serum level of total cholesterol and triglycerides, similar to normolipidemic rats and hyperlipidemic rats treated with simvastatin (30 mg/kg of body weight) and ciprofibrate (2 mg/kg of body weight). Moreover, the treatment with ExCA also decreased malondialdehyde serum level in the hyperlipidemic rats. The body weight and organ mass were unmodified by ExCA in hyperlipidemic rats, except an increase of liver mass; however, the hepatic enzymes, alanine aminotransferase and aspartate aminotransferase, were unchanged. Together, these results confirm the potential value ofCampomanesia adamantiumroot for lowering lipid peroxidation and lipid serum level, improving risk factors for cardiometabolic diseases development.

scholarly journals Anticarcinogenic potential of ethanol extract of Indigofera cordifolia Roth. (Fabales: Fabaceae) on diethylnitrosamine induced hepatocarcinogenesis in rats

2017 ◽  
Vol 4 (7) ◽  
pp. 53-66 ◽  
Author(s):  
Raju Senthil Kumar ◽  
Gopal Murugananthan ◽  
Balasubramanian Rajkapoor
Keyword(s):  
Lipid Peroxidation ◽  
Body Weight ◽  
Free Radical ◽  
Protein Content ◽  
Radical Scavenging ◽  
Liver Weight ◽  
Free Radical Scavenging ◽  
Tumour Markers ◽  
The Body ◽  
Relative Liver Weight

Indigofera cordifolia Roth. (Fabales: Fabaceae) has been widely used in Indian system of medicine to treat various disorders. Earlier studies showed that I. cordifolia (EIC) possess antioxidant, free radical scavenging and antitumour activities. The present investigation was designed to evaluate the anticarcinogenic potential of EIC against diethylnitrosamine (DEN) induced hepatocellular carcinoma in male Wistar rats. Hepatocarcinogenesis was induced by a single intraperitoneal administration of DEN (200 mg/kg) and the carcinogenic effect was promoted by phenobarbital given through drinking water for 16 successive weeks. EIC at the dose of 200 and 400 mg/kg/day were administered orally for the entire study period. After the end of experimental period, changes in body weight, the weight of liver, relative liver weight, lipid peroxidation, antioxidant, serum hepatic parameters, tumour markers, DNA, RNA and protein content were analysed. Treatment with EIC significantly increased the body weight (P < 0.01-0.001), reduced the liver weight and relative liver weight (P < 0.01-0.001), restored the altered serum hepatic parameters (P < 0.001), down-regulated the serum tumour markers such as alpha-fetoprotein and carcinoembryonic antigen (P < 0.001) when compared to DEN group. EIC treatment restored the antioxidant enzymes and significantly reduced the lipid peroxidation in DEN-treated animals (P < 0.001). EIC treatment also significantly reduced the elevated levels of nucleic acid levels and restored the protein content in liver tissues (P < 0.001). We investigated the anticarcinogenic potential of EIC against DEN-induced HCC in rats. Chemoprotective effect of the extract might be related with antioxidant, free radical scavenging and reduction of lipid peroxidation. The results suggested that EIC would be a potent anticarcinogenic agent inhibiting DEN-induced hepatic carcinoma.

scholarly journals IN VITRO ANTIOXIDANT CAPACITY AND FREE RADICAL SCAVENGING ACTIVITIES OF CARDIOSPERMUM HALICACABUM LINN.

2018 ◽  
Vol 11 (7) ◽  
pp. 421
Author(s):  
Savitha G ◽  
Vishnupriya V ◽  
Surapaneni Krishnamohan
Keyword(s):  
Lipid Peroxidation ◽  
Free Radical ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Free Radical Scavenging ◽  
The Body ◽  
Ferrous Ion ◽  
Harmful Effects ◽  
Radical Scavenging Assay ◽  
Free Radical Scavenging Activities

Objective: Free radicals and oxidants are produced in the body during normal cellular metabolism and exposure to pollutions and ionizing radiations. They exert beneficial and harmful effects to the human body. Harmful effects of these radicals are destroyed by either antioxidants of our body in situ or antioxidants which are supplied through foods. Hence, the aim of this present study is to assess the antioxidant and free radical scavenging capacity of the easily available edible plant Cardiospermum halicacabum Linn. (Mudakathan keerai).Methods: Antioxidant and free radical scavenging activities of the aerial parts extract of C. halicacabum was determined by 2,2-diphenyl-1- picrylhydrazyl (DPPH) radical scavenging assay, 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging assay, ferrous ion chelating assay, nitric oxide (NO) radical scavenging assay, superoxide radical scavenging assay, hydroxyl radical scavenging assay, and lipid peroxidation assay.Results: Remarkable free radical scavenging potential was observed in the ethanolic extract of C. halicacabum with IC50 values on all tested radicals, namely DPPH (IC50=34.06 μg/ml), ABTS (IC50=21.45 μg/ml), ferrous ion chelating (IC50=19.56 μg/ml), NO (IC50=25.16 μg/ml), superoxide (IC50=35.16 μg/ml), hydroxyl (IC50=28.56 μg/ml), and lipid peroxidation (IC50=33.12 μg/ml). The results revealed that ethanolic extract of C. halicacabum Linn. has significant antioxidant activity.Conclusion: The present study suggested that the aerial part extract of C. halicacabum serves as a good source of natural antioxidants.

TOTAL POLYPHENOL CONTENT, FREE RADICAL SCAVENGING, ALPHA-GLUCOSIDASE INHIBITION CAPACITY, THE EFFICACY IN CONTROLLING BLOOD GLUCOSE OF DIABETIC RATS OF THE MIXTURE VOS EXTRACTED FROM THE LEAVES OF CLEISTOCALYX OPERCULATUS, PSIDIUM GUAJAVA AND NELUMBO NUCIFERA

2013 ◽  
pp. 50-55
Author(s):  
Tuyet Mai Truong ◽  
Thi Lam Nguyen ◽  
Lan Anh Pham ◽  
Hoang Kien Truong
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Radical Scavenging ◽  
Psidium Guajava ◽  
Free Radical Scavenging ◽  
Diabetic Mice ◽  
Cleistocalyx Operculatus ◽  
Prevention And Treatment ◽  
Treatment Of Diabetes ◽  
Alpha Glucosidase

Objective: Plant polyphenols have antioxidant capacity and alpha-glucosidase inhibition to supporting for prevention and treatment of diabetes. Materials and Method: Present study was conducted to determine the content of total polyphenols, free radical scavenging and alpha-glucosidase inhibition of the VOS mixture that extracted from leaves (Voi leaves - Cleistocalyx operculatus (V), Oi leaves - Psidium guajava (O), Sen leaves - Nelumbo nucifera (S)). Results: The efficacy of blood glucose controlling in diabetic mice was investigated. After 8 weeks of administration with 200 mg VOS/kg body weight and 400 mg VOS/kg body weight, VOS diabetic mice had significantly reduced blood glucose level as compared to control diabetic mice. VOS diabetic mice with 400 mg dosage are lower in blood glucose levels than that of the diabetic mice with 200 mg. Also, the significant reducing in HbA1c was observed in VOS diabetic mice as compared with control diabetic mice. Conclusion: VOS-product extracted from Cleistocalyx operculatus leaves, guava leaves, lotus leaves might be considered as a safe product and to be a potential product in the supporting of prevention and treatment of diabetes.

Investigation of Free Radical Scavenging Enzyme Activities and Lipid Peroxidation in Human Placental Tissues With Miscarriage

2006 ◽  
Vol 13 (5) ◽  
pp. 384-388 ◽  
Author(s):  
Aydan Biri ◽  
Mustafa Kavutcu ◽  
Nuray Bozkurt ◽  
Erdinç Devrim ◽  
Nilhan Nurlu ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Free Radical ◽  
Enzyme Activities ◽  
Radical Scavenging ◽  
Free Radical Scavenging

Desiccation sensitivity and activities of free radical-scavenging enzymes in recalcitrant Theobroma cacao seeds

1999 ◽  
Vol 9 (3) ◽  
pp. 209-217 ◽  
Author(s):  
Changrun Li ◽  
Wendell Q. Sun
Keyword(s):  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Water Content ◽  
Theobroma Cacao ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Desiccation Sensitivity ◽  
Critical Water Content ◽  
Scavenging Enzymes ◽  
Water Contents

AbstractMature and immature axes of Theobroma cacao (cocoa) seeds tolerated desiccation under a rapid-drying regime to critical water contents of 1.0 and 1.7 g g-1 dw, respectively. These critical water contents corresponded to water contents below which activities of free radical-scavenging enzymes (ascorbate peroxidase, peroxidase and superoxide dismutase) decreased rapidly during desiccation. The decline in axis viability below the critical water content was correlated with sharp increases in lipid peroxidation and cellular leakage. Cotyledon tissues were more desiccation-tolerant than axes, with a low critical water content of 0.24 g g–1dw. Desiccation sensitivity in cotyledon tissues was also correlated with the decrease in superoxide dismutase activity and increased lipid peroxidation products. However, in the cotyledons, no ascorbate peroxidase activity was detected at any water content, and peroxidase activity was gradually reduced as desiccation proceeded. Cocoa embryonic axes contained large amounts of sucrose, raffinose and stachyose but only traces of reducing monosaccharides. Desiccation sensitivity of recalcitrant cocoa axes did not appear to be due to the lack of sugar-related protective mechanisms during desiccation, and it was more likely related to the decrease of enzymic protection against desiccation-induced oxidative stresses.

Psoralidin exerts anti-tumor, anti-angiogenic, and immunostimulatory activities in 4T1 tumor‐bearing balb/c mice

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Davar Amani ◽  
Elham Shakiba ◽  
Ehsan Motaghi ◽  
Hiva Alipanah ◽  
Mahshad Jalalpourroodsari ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Body Weight ◽  
Serum Level ◽  
Tumor Volume ◽  
Treated Group ◽  
The Body ◽  
Ki 67 ◽  
Tumor Bearing ◽  
Anti Cancer ◽  
Ifn Γ

Abstract Background Psoralidin as a compound of the Psoralea corylifolia seeds exhibited several anti-cancer potentials in various cancers. Materials and methods In this study, 4T1 tumor‐bearing Balb/c mice were treated by intraperitoneal administration of Psoralidin, and Paraffin, as a control group to investigate anti-tumor, anti-angiogenic, and immunostimulatory activities in breast cancer. Body weight and tumor volume measurement were performed. Hematoxylin and Eosin (H&E) staining as well as immunohistochemistry for Ki-67, CD31 and VEGF markers were conducted. In addition, ELISA assay was performed for evaluating the serum level of IFN-γ and IL-4. Moreover, real time assay was performed to evaluate the expression of angiogenesis and immunostimulatory related genes. Results There were no significant changes in the body weight of all animal groups. The anti-cancer effects of Psoralidin were significantly observed after 24 days of the last treatment, confirmed by smaller tumor volume and also H&E staining. The expression level of Ki‐67, CD31 and VEGF were significantly decreased in tumor tissues of the Psoralidin-treated group in comparison with Paraffin-treated group. Moreover, there was a significant reduction in the serum level of IL-4 in tumor-bearing mice after Psoralidin treatment while the serum level of IFN-γ was significantly augmented in all groups. Moreover, the reduction in expression of VEGF-a and IL-1β was observed. Interestingly Psoralidin treatment led to expression increase of FOXp3. Conclusions Psoralidin shows the anti-cancer potential in an animal model of breast cancer; however, further studies are recommended to elucidate its mechanisms of action.

Effect of Bitters on the Body Weight, Lipid Profile, Catalase and Lipid Peroxidation in Experimental Animals

2012 ◽  
Vol 13 (1) ◽  
pp. 62-66 ◽  
Author(s):  
M.A. Alabi ◽  
R.M. Sunday ◽  
T. Olowokere ◽  
F.A. Kareem ◽  
F. Osanaiye
Keyword(s):  
Lipid Peroxidation ◽  
Body Weight ◽  
Lipid Profile ◽  
The Body ◽  
Experimental Animals

Antioxidant and Hepatoprotective Effects of Orthosiphon stamineus Benth.

2007 ◽  
Vol 35 (01) ◽  
pp. 115-126 ◽  
Author(s):  
Mun Fei Yam ◽  
Rusliza Basir ◽  
Mohd. Zaini Asmawi ◽  
Zhari Ismail
Keyword(s):  
Lipid Peroxidation ◽  
Free Radical ◽  
Liver Toxicity ◽  
Radical Scavenging ◽  
Water Extract ◽  
Liver Function Tests ◽  
Hepatoprotective Activity ◽  
Free Radical Scavenging ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Orthosiphon Stamineus

Orthosiphon stamineus (OS), Benth. (Lamiaceae) is widely used in Malaysia for treatments of various kidney and liver ailments. In the experiment, DPPH• radicals scavenging, Fe 3+-induced lipid peroxidation inhibiting activities and trolox equivalent antioxidant capacity (TEAC) of methanol/water extract of Orthosiphon stamineus (SEOS) were determined. The results indicated that SEOS exhibited antioxidant, lipid peroxidation inhibition and free radical scavenging activities. The hepatoprotective activity of the SEOS was studied using CCl 4-induced liver toxicity in rats. The activity was assessed by monitoring liver function tests through the measurement of alanine transaminase (ALT) and aspartate transaminase (AST). Furthermore, hepatic tissues were also subjected to histopathological studies. Pretreatment of SEOS (125, 250, 500 and 1000 mg/kg p.o.) dose-dependently reduced the necrotic changes in rat liver and inhibited the increase of serum ALT and AST activities. The results of the present study indicated that the hepatoprotective effect of Orthosiphon stamineus might be ascribable to its antioxidant and free radical scavenging property.

scholarly journals Constituents of the Stem of Nauclea orientalis

2015 ◽  
Vol 10 (11) ◽  
pp. 1934578X1501001 ◽  
Author(s):  
Phan Thi Anh Dao ◽  
Tran Le Quan ◽  
Nguyen Thi Thanh Mai
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Free Radical ◽  
Spectroscopic Data ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Propanoic Acid ◽  
Coumaric Acid ◽  
Aloe Emodin ◽  
Inhibitory Activities

A new α-pinene derivative (1) was isolated from the stem of Nauclea orientalis (L.) L. (Rubiaceae), together with twelve known compounds, including four terpenoids, loganetin (2), loganin (3), sweroside (4), grandifloroside (5), four simple phenols, methyl protocatechuate (6), trans- p-coumaric acid (7), 3-(2,4-dihydroxylphenyl)propanoic acid (8), methyl 3-(2,4-dihydroxylphenyl)propanoate (9), two coumarin glucosides, skimmin (10), adicardin (11), an anthraquinone, aloe emodin (12), and a lignan, (+)-pinoresinol (13). Their structures were elucidated on the basis of spectroscopic data. All compounds were tested for DPPH free radical scavenging and lipid peroxidation inhibitory activities. Among them, compounds 5 and 6 displayed strong antioxidant activity with IC50 values of 6.6 and 67.9 μM, 12.4 and 813.0 μM, respectively.

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