scholarly journals In Vitro Cytotoxicity of Mesoporous SiO2@Eu(OH)3Core-Shell Nanospheres in MCF-7

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
M. Atif ◽  
Muhammad Fakhar-e-Alam ◽  
Najeeb Abbas ◽  
Maqsood A. Siddiqui ◽  
Anees A. Ansari ◽  
...  
Keyword(s):  
Morphological Analysis ◽  
Morphological Changes ◽  
Sol Gel ◽  
Average Diameter ◽  
In Vitro Cytotoxicity ◽  
Core Shell ◽  
Clinical Approach ◽  
Transmission Electron ◽  
Mcf 7

Initially, the sample was synthesized by a modified sol-gel process. Morphological analysis of growth SiO2@Eu(OH)3was confirmed by applying field emission transmission electron microscopy (high and low resolution FETEM). The images confirmed the average diameter of mesoporous SiO2@Eu(OH)3core-shell nanospheres (~392–400 nm) with a silica core of ~230 nm in diameter and a shell composed of europium hydroxide ~162 nm (thickness). Moreover, an absorption band at 280 nm was confirmed which initiates from the europium hydroxide. The photoluminescence spectrum of the nanosphere was also recorded at ambient temperature under the excitation of 3.82 eV. Cytotoxic studies in vitro were performed by applying MTT, NR assays, and morphological analysis. Morphological changes and % loss in cellular viability was assessed in human breast cancer cells (MCF-7) labeled with mesoporous SiO2@Eu(OH)3core-shell nanospheres at different concentrations ranging from 10 µg/mL to 200 µg/mL. Current study demonstrates the quite rational strategy which might be useful in future clinical approach/applications.

Fe3O4@TiO2 Nanocomposites for Magnetic Targeting Sonodynamic Therapy of Cancer

2014 ◽  
Vol 636 ◽  
pp. 129-132 ◽  
Author(s):  
Song Shen ◽  
Bei Ding ◽  
Lin Wu ◽  
Xue Yong Qi ◽  
Yan Ru Ge
Keyword(s):  
Sol Gel ◽  
Average Diameter ◽  
In Vitro Cytotoxicity ◽  
Spherical Nanoparticles ◽  
X Ray Diffraction ◽  
Sonodynamic Therapy ◽  
X Ray ◽  
Transmission Electron ◽  
Good Biocompatibility

Uniform Fe3O4@TiO2 nanocomposites were successfully synthesized via a sol-gel method and then characterized by transmission electron microscope (TEM) and X-ray diffraction (XRD). The obtained composites were monodispersed spherical nanoparticles with average diameter of ~ 230 nm. In vitro cytotoxicity experiment, Fe3O4@TiO2 nanocomposites exhibited good biocompatibility and obvious sonodynamic therapy (SDT) effect. Over 50% cells were killed when the cells were treated with Fe3O4@TiO2 and ultrasound for 1 min. The results indicated the potential of Fe3O4@TiO2 for targeted sonodynamic therapy of tumor.

scholarly journals Synthesis, characterization and in vitro cytotoxicity study of Co and Ni ferrite nanoparticles prepared by sol-gel method

2021 ◽  
Vol 3 (7) ◽  
Author(s):  
Alexandre Pancotti ◽  
Dener Pereira Santos ◽  
Dielly Oliveira Morais ◽  
Mauro Vinícius de Barros Souza ◽  
Débora R. Lima ◽  
...  
Keyword(s):  
Photoelectron Spectroscopy ◽  
Sol Gel ◽  
Test Sample ◽  
In Vitro Cytotoxicity ◽  
Gel Method ◽  
X Ray ◽  
Sol Gel Method ◽  
Transmission Electron ◽  
Crystallite Sizes

AbstractIn this study, we report the synthesis and characterization of NiFe2O4 and CoFe2O4 nanoparticles (NPs) which are widely used in the biomedical area. There is still limited knowledge how the properties of these materials are influenced by different chemical routes. In this work, we investigated the effect of heat treatment over cytotoxicity of cobalt and niquel ferrites NPs synthesized by sol-gel method. Then the samples were studied using transmission electron microscopy (TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), vibrating sample magnetometer (VSM), Fourier Transform Infrared Spectroscopy Analysis (FTIR), and X-ray fluorescence (XRF). The average crystallite sizes of the particles were found to be in the range of 20–35 nm. The hemocompatibility (erythrocytes and leukocytes) was checked. Cytotoxicity results were similar to those of the control test sample, therefore suggesting hemocompatibility of the tested materials.

Biocompatible luminescent nanoparticles on the basis of calcium phosphate

2011 ◽  
Vol 1355 ◽  
Author(s):  
Sofia Dembski ◽  
Moritz Milde ◽  
Emeline Dassonneville ◽  
Carsten Gellermann ◽  
Torsten Klockenbring ◽  
...  
Keyword(s):  
Inductively Coupled Plasma ◽  
Sol Gel ◽  
Optical Emission ◽  
Emission Spectrometry ◽  
Core Shell ◽  
Shell Nanoparticles ◽  
Inductively Coupled ◽  
Transmission Electron ◽  
Core Shell Nanoparticles

ABSTRACTLuminescent lanthanide doped SiO2/Hydroxylapatite (HAp) core/shell nanoparticles (NPs) were synthesized by sol-gel technology. The resulting NPs exhibited an amorphous SiO2 core and a crystalline luminescent shell. The formation of the HAp layer was possible at pH 8.5. The characterization of the resulting NPs was done by transmission electron microscopy, X-ray diffraction analysis, inductively-coupled plasma combined with optical emission spectrometry, and photoluminescence spectroscopy. Additionally, the newly developed SiO2/HAp:Ln3+ core/shell NPs were tested for their biocompatibility, e. g. by an in vitro cell culture based assay.

scholarly journals GANODERMA LUCIDUM-ORIENTAL MUSHROOM MEDIATED SYNTHESIS OF GOLD NANOPARTICLES CONJUGATED WITH DOXORUBICIN AND EVALUATION OF ITS ANTICANCER POTENTIAL ON HUMAN BREAST CANCER MCF-7/DOX CELLS

2017 ◽  
Vol 9 (9) ◽  
pp. 267 ◽  
Author(s):  
D. S. Ranjith Santhosh Kumar ◽  
P. Senthilkumar ◽  
L. Surendran ◽  
B. Sudhagar
Keyword(s):  
Breast Cancer ◽  
Gold Nanoparticles ◽  
Mrna Expression ◽  
In Vitro Cytotoxicity ◽  
Abcb1 Gene ◽  
Transmission Electron ◽  
Visible Spectra ◽  
Mcf 7

Objective: The present investigations are to mycosynthesis and characterization of gold nanoparticles conjugated with doxorubicin and evaluated anticancer activity.Methods: The characterization of the gold nanoparticles using ultraviolet–visible spectroscopy. FTIR investigations were carried out to find and read the functional group responsible designed at the bioconversion of gold ions and crystalline arrangement of gold nanoparticles was detected in the XRD study. The gold nanoparticles conjugated with doxorubicin were treated against MCF-7-dox resisted breast cancer cells and observed the in vitro cytotoxicity by MTT assay, SCGE (Comet), Apoptosis and Mito-potential assay. Further more we determinate the mRNA expression of ABCB1 gene and cDNA was synthesized from the mRNA for amplification of the ABCB1 gene corresponding to the specific primer.Results: Surface Plasmon resonance showed the development of gold nanoparticles in UV–Visible spectra at 537 nm. The synthesized gold nanoparticles were polydisperse spherical and it was determined by EDAX and stabilized in the solution to the spherical shapes further confirmed by High-resolution transmission electron microscope analysis designate in the reading of 2–100 nm. The anticancer assays were given significant results and the mRNA expression of ABCB1 gene and cDNA was amplified as directly proportional to the expression of ABCB1 gene.Conclusion: We propose that gold nanoparticles synthesized and conjugated with doxorubicin from G. lucidum might be a significant resource of drug delivery for anti-cancer preparation that may advantage breast cancer treatment.

Correlation Between Cellular Functions and Morphological Changes of Human Trophoblast in Vitro

1975 ◽  
Vol 33 ◽  
pp. 600-601
Author(s):  
John C. Garancis ◽  
Robert O. Hussa ◽  
Michael T. Story ◽  
Donald Yorde ◽  
Roland A. Pattillo
Keyword(s):  
Electron Microscopy ◽  
Cellular Proliferation ◽  
Morphological Changes ◽  
Culture Fluid ◽  
Cellular Functions ◽  
Human Trophoblast ◽  
Transmission Electron ◽  
Marked Activity ◽  
Malignant Trophoblast

Human malignant trophoblast cells in continuous culture were incubated for 3 days in medium containing 1 mM N6-O2'-dibutyryl cyclic adenosine 3':5'-monophosphate (dibutyryl cyclic AMP) and 1 mM theophylline. The culture fluid was replenished daily. Stimulated cultures secreted many times more chorionic gonadotropin and estrogens than did control cultures in the absence of increased cellular proliferation. Scanning electron microscopy revealed remarkable surface changes of stimulated cells. Control cells (not stimulated) were smooth or provided with varying numbers of microvilli (Fig. 1). The latter, usually, were short and thin. The surface features of stimulated cells were considerably different. There was marked increase of microvilli which appeared elongated and thick. Many cells were covered with confluent polypoid projections (Fig. 2). Transmission electron microscopy demonstrated marked activity of cytoplasmic organelles. Mitochondria were increased in number and size; some giant forms with numerous cristae were observed.

scholarly journals Mucoadhesive Nanoparticles for Drug Delivery to the Anterior Eye

Nanomaterials ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 1400
Author(s):  
Nicole Mangiacotte ◽  
Graeme Prosperi-Porta ◽  
Lina Liu ◽  
Megan Dodd ◽  
Heather Sheardown
Keyword(s):  
Morphological Analysis ◽  
Phenylboronic Acid ◽  
Spherical Particles ◽  
Target Tissue ◽  
Hydroxyethyl Methacrylate ◽  
Potential Analysis ◽  
Transmission Electron ◽  
Nanoparticle System ◽  
Zeta Potential Analysis

While the use of topical drops for the delivery of drugs to the anterior of the eye is well accepted, it is far from efficient with as little as 5% of the drug instilled on the eye actually reaching the target tissue. The ability to prolong the residence time on the eye is desirable. Based on the acceptability of 2-hydroxyethyl methacrylate based polymers in contact lens applications, the current work focuses on the development of a poly(2-hydroxyethyl methacrylate (HEMA)) nanoparticle system. The particles were modified to allow for degradation and to permit mucoadhesion. Size and morphological analysis of the final polymer products showed that nano-sized, spherical particles were produced. FTIR spectra demonstrated that the nanoparticles comprised poly(HEMA) and that 3-(acrylamido)phenylboronic acid (3AAPBA), as a mucoadhesive, was successfully incorporated. Degradation of nanoparticles containing N,N′-bis(acryloyl)cystamine (BAC) after incubation with DL-dithiothreitol (DTT) was confirmed by a decrease in turbidity and through transmission electron microscopy (TEM). Nanoparticle mucoadhesion was shown through an in-vitro zeta potential analysis.

scholarly journals Biocompatibility Characteristics of Titanium Coated with Multi Walled Carbon Nanotubes—Hydroxyapatite Nanocomposites

Materials ◽  
2019 ◽  
Vol 12 (2) ◽  
pp. 224 ◽  
Author(s):  
Jung-Eun Park ◽  
Yong-Seok Jang ◽  
Tae-Sung Bae ◽  
Min-Ho Lee
Keyword(s):  
Electron Microscopy ◽  
Carbon Nanotubes ◽  
Sol Gel ◽  
Pure Titanium ◽  
Cell Proliferation And Differentiation ◽  
Transmission Electron ◽  
Proliferation And Differentiation ◽  
Multi Walled Carbon Nanotubes ◽  
Walled Carbon Nanotubes

Multi walled carbon nanotubes-hydroxyapatite (MWCNTs-HA) with various contents of MWCNTs was synthesized using the sol-gel method. MWCNTs-HA composites were characterized by X-ray diffraction (XRD) and transmission electron microscopy (TEM). HA particles were generated on the surface of MWCNT. Produced MWCNTs-HA nanocomposites were coated on pure titanium (PT). Characteristic of the titanium coated MWCNTs-HA was evaluated by field-emission scanning electron microscopy (FE-SEM) and XRD. The results show that the titanium surface was covered with MWCNTs-HA nanoparticles and MWCNTs help form the crystalized hydroxyapatite. Furthermore, the MWCNTs-HA coated titanium was investigated for in vitro cellular responses. Cell proliferation and differentiation were improved on the surface of MWCNT-HA coated titanium.

Monodisperse Fe3O4/Fe Core/Shell Nanoparticles with Enhanced Magnetic Property

2011 ◽  
Vol 306-307 ◽  
pp. 410-415
Author(s):  
Li Sun ◽  
Fu Tian Liu ◽  
Qi Hui Jiang ◽  
Xiu Xiu Chen ◽  
Ping Yang
Keyword(s):  
Average Diameter ◽  
Chemical Precipitation ◽  
Precipitation Method ◽  
Core Shell ◽  
X Ray Diffraction ◽  
Shell Nanoparticles ◽  
X Ray ◽  
Transmission Electron ◽  
Shell Particles ◽  
Core Shell Nanoparticles

Core/shell type nanoparticles with an average diameter of 20nm were synthesized by chemical precipitation method. Firstly, Monodisperse Fe3O4 nanoparticles were synthesized by solvethermal method. FeSO4ž7H2O and NaBH4 were respectively dissolved in distilled water, then moderated Fe3O4 particles and surfactant(PVP) were ultrasonic dispersed into the FeSO4ž7H2O solution. The resulting solution was stirred 2 h at room temperature. Fe could be deposited on the surface of monodispersed Fe3O4 nanoparticles to form core-shell particles. The particles were characterized by using various experimental techniques, such as transmission electron microscopy (TEM), X-ray diffraction (XRD), AGM and DTA. The results suggest that the saturation magnetization of the nanocomposites is 100 emu/g. The composition of the samples show monodisperse and the sides of the core/shell nanoparticles are 20-30nm. It is noted that the formation of Fe3O4/Fe nanocomposites magnetite nanoparticles possess superparamagnetic property.

scholarly journals Synthesis and In Vitro Antitumor Activity of Novel Bivalent β-Carboline-3-carboxylic Acid Derivatives with DNA as a Potential Target

2018 ◽  
Vol 19 (10) ◽  
pp. 3179 ◽  
Author(s):  
Hongling Gu ◽  
Na Li ◽  
Jiangkun Dai ◽  
Yaxi Xi ◽  
Shijun Wang ◽  
...  
Keyword(s):  
Antitumor Activity ◽  
Cell Apoptosis ◽  
Docking Studies ◽  
In Vitro Cytotoxicity ◽  
Dependent Manner ◽  
Cycle Arrest ◽  
Dna Binding Affinity ◽  
Dose Dependent ◽  
Mcf 7

A series of novel bivalent β-carboline derivatives were designed and synthesized, and in vitro cytotoxicity, cell apoptosis, and DNA-binding affinity were evaluated. The cytotoxic results demonstrated that most bivalent β-carboline derivatives exhibited stronger cytotoxicity than the corresponding monomer against the five selected tumor cell lines (A549, SGC-7901, Hela, SMMC-7721, and MCF-7), indicating that the dimerization at the C3 position could enhance the antitumor activity of β-carbolines. Among the derivatives tested, 4B, 6i, 4D, and 6u displayed considerable cytotoxicity against A549 cell line. Furthermore, 4B, 6i, 4D, and 6u induced cell apoptosis in a dose-dependent manner, and caused cell cycle arrest at the S and G2/M phases. Moreover, the levels of cytochrome C in mitochondria, and the expressions of bcl-2 protein, decreased after treatment with β-carbolines, which indicated that 6i and 6u could induce mitochondria-mediated apoptosis. In addition, the results of UV-visible spectral, thermal denaturation, and molecular docking studies revealed that 4B, 6i, 4D, and 6u could bind to DNA mainly by intercalation.

Paracrystalline bundles of large tubules, induced in vitro by Mebendazole in Cysticercus cellulosae

Parasitology ◽  
1981 ◽  
Vol 83 (3) ◽  
pp. 513-518 ◽  
Author(s):  
J. P. Laclette ◽  
Marie Therese Merchant ◽  
Kaethe Willms ◽  
L. Cañedo
Keyword(s):  
Electron Microscopy ◽  
Culture Medium ◽  
Morphological Changes ◽  
Bladder Wall ◽  
Secretory Cells ◽  
External Diameter ◽  
Nematode Larvae ◽  
Transmission Electron ◽  
Cysticercus Cellulosae

SUMMARYThe effect of the anthelmintic Mebendazole on Cysticercus cellulosae maintained in culture medium was studied by transmission electron microscopy. In addition to the well-known morphological changes induced by Mebendazole in other cestode and nematode larvae, it also induced the cytoplasmic appearance of paracrystalline bundles in the secretory cells of the bladder wall. These bundles were formed by groups of large parallel tubules arranged in a hexagonal-like pattern. The tubules, which had an external diameter of about 50 nm and a length that might exceed 5 μm, were surrounded by a matrix and a distance between neighbouring tubules of 80–120 nm centre to centre was estimated. The tubules were stable to colchicine and low temperature. The temporary appearance of bundles is described and some alternative explanations on their origin are advanced.

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