scholarly journals Development and Physicochemical Characterization of Desonide-Loaded Nanocapsule Suspensions

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
M. B. Antonow ◽  
R. Lorenzoni ◽  
G. M. Barbosa ◽  
A. F. Ourique ◽  
P. Gomes ◽  
...  
Keyword(s):  
Particle Size ◽  
High Performance Liquid Chromatography ◽  
High Performance ◽  
Release Kinetics ◽  
Physicochemical Characterization ◽  
Eudragit S100 ◽  
Franz Diffusion Cells ◽  
Negative Zeta Potential

Desonide is a topical corticosteroid that has been used for more than 30 years; however, its prolonged use can induce several side effects, affecting dermis and epidermis. The present work consists of development desonide-loaded nanocapsule suspensions (D-NC) using different polymers (Eudragit S100® or Eudragit L100®) and desonide-loaded lipid-core nanocapsules (D-LNC). They were formulated by interfacial deposition using the preformed polymer method and all formulations showed negative zeta potential and adequate nanotechnological characteristics (particle size 161–202 nm, polydispersity index < 0.20). Simple and sensitive methods using high-performance liquid chromatography (HPLC) were developed to quantify desonide in LNC and to study its release kinetics. The method was linear, specific, precise, and exact and therefore can be applied in quantification of D-NC and D-LNC. We evaluatedin vitromethods for drug release (dissolution, Franz diffusion cells, and dialysis sac) and we use mathematical models (monoexponential, biexponential, and Korsmeyer-Peppas) to show release kinetics from this system.

Folate Tethered Gd2O3 Nanoparticles Exhibit Photoactive Antimicrobial Effects and pH Responsive Delivery of 5-fluorouracil into MCF-7 Cells

2019 ◽  
Vol 9 (1) ◽  
pp. 58-68
Author(s):  
Sambhaji R. Bamane ◽  
Vijay J. Sawant
Keyword(s):  
Anticancer Activity ◽  
Release Kinetics ◽  
Physicochemical Characterization ◽  
Microscopic Analysis ◽  
Ph Responsive ◽  
Antibacterial Screening ◽  
Gd2o3 Nanoparticles ◽  
Mcf 7

Background: Destroying hydrophobicity and increasing bioavailability of anticancer drugs is emerging field in biomedical nanotherapy. </P><P> Methods: The porous and oval shaped Gd2O3 gadolinite nanoparticles were synthesized and surface functionalized with folate groups using wet coprecipitation method. The presence of spinal nanophase with Gd2O3 lattice inside nanoparticles was confirmed by the use of XRD pattern and supportive FTIR spectrum. XRD data of nanocomposites proved the spinal core of gadolinite phase even after surface tailoring. These porous nanoparticles were loaded with anticancer drug 5-flurouracil for enhancement of anticancer activity on breast cancer MCF-7 cells. The elemental, optical, morphological and phase physicochemical characterization of the nanomaterials were performed using techniques such as PL, FTIR, XRD spectrometry, TGA thermal analysis, SEM and TEM microscopic analysis. The photoactive biocompatibility of nanohybrids was elaborated on gram positive S. aureus bacteria by agar well antibacterial screening in dark and light. </P><P> Results: The nanocomposites not only exhibited photoactive biocompatibility but also pH responsive in vitro delivery applied for anticancer therapy on the basis of spectrometric assay following sustained release with zero order Peppas release kinetics. Conclusion: The nanocomposites exhibited higher anticancer activity on MCF-7 cells than free drug and nanohybrids after in vitro MTT assay. These 5-FU loaded folate targeted luminescent and photoactive nanocomposites with gadolinite core find applications in the future biomedical cell-particle interface.

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