scholarly journals Transcriptome Analyses Reveal Lipid Metabolic Process in Liver Related to the Difference of Carcass Fat Content in Rainbow Trout (Oncorhynchus mykiss)

2016 ◽  
Vol 2016 ◽  
pp. 1-10 ◽  
Author(s):  
Guo Hu ◽  
Wei Gu ◽  
Peng Sun ◽  
Qingli Bai ◽  
Bingqian Wang
Keyword(s):  
Rainbow Trout ◽  
Lipid Metabolism ◽  
Oncorhynchus Mykiss ◽  
Fat Content ◽  
Metabolic Process ◽  
Farm Animals ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Whole Transcriptome Sequencing ◽  
The Difference ◽  
The Cost

Excessive accumulation of carcass fat in farm animals, including fish, has a significant impact on meat quality and on the cost of feeding. Similar to farmed animals and humans, the liver can be considered one of the most important organs involved in lipid metabolism in rainbow trout (Oncorhynchus mykiss). RNA-seq based whole transcriptome sequencing was performed to liver tissue of rainbow trout with high and low carcass fat content in this study. In total 1,694 differentially expressed transcripts were identified, including many genes involved in lipid metabolism, such asL-FABP,adiponectin,PPAR-α, PPAR-β,andIGFBP1a. Evidence presented in this study indicated that lipid metabolic process in liver may be related to the difference of carcass fat content. The relevance ofPPAR-αandPPAR-βas molecular markers for fat storage in liver should be worthy of further investigation.

scholarly journals Genistein Induces Adipogenic and Autophagic Effects in Rainbow Trout (Oncorhynchus mykiss) Adipose Tissue: In Vitro and In Vivo Models

2020 ◽  
Vol 21 (16) ◽  
pp. 5884
Author(s):  
Sara Balbuena-Pecino ◽  
Esmail Lutfi ◽  
Natàlia Riera-Heredia ◽  
Esther Gasch-Navalón ◽  
Emilio J. Vélez ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Rainbow Trout ◽  
Lipid Metabolism ◽  
Oncorhynchus Mykiss ◽  
Fatty Acid Synthase ◽  
Hormone Sensitive Lipase ◽  
In Vivo Models ◽  
Rainbow Trout Oncorhynchus Mykiss

Soybeans are one of the most used alternative dietary ingredients in aquafeeds. However, they contain phytoestrogens like genistein (GE), which can have an impact on fish metabolism and health. This study aimed to investigate the in vitro and in vivo effects of GE on lipid metabolism, apoptosis, and autophagy in rainbow trout (Oncorhynchus mykiss). Primary cultured preadipocytes were incubated with GE at different concentrations, 10 or 100 μM, and 1 μM 17β-estradiol (E2). Furthermore, juveniles received an intraperitoneal injection of GE at 5 or 50 µg/g body weight, or E2 at 5 µg/g. In vitro, GE 100 μM increased lipid accumulation and reduced cell viability, apparently involving an autophagic process, indicated by the higher LC3-II protein levels, and higher lc3b and cathepsin d transcript levels achieved after GE 10 μM. In vivo, GE 50 µg/g upregulated the gene expression of fatty acid synthase (fas) and glyceraldehyde-3-phosphate dehydrogenase in adipose tissue, suggesting enhanced lipogenesis, whereas it increased hormone-sensitive lipase in liver, indicating a lipolytic response. Besides, autophagy-related genes increased in the tissues analyzed mainly after GE 50 µg/g treatment. Overall, these findings suggest that an elevated GE administration could lead to impaired adipocyte viability and lipid metabolism dysregulation in rainbow trout.

scholarly journals Selection for muscle fat content and triploidy affect flesh quality in pan-size rainbow trout, Oncorhynchus mykiss

Aquaculture ◽  
2015 ◽  
Vol 448 ◽  
pp. 569-577 ◽  
Author(s):  
Florence Lefevre ◽  
Mireille Cardinal ◽  
Jérôme Bugeon ◽  
Laurent Labbe ◽  
Françoise Medale ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Fat Content ◽  
Flesh Quality ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Selection For

Intracerebroventricular ghrelin treatment affects lipid metabolism in liver of rainbow trout (Oncorhynchus mykiss)

2016 ◽  
Vol 228 ◽  
pp. 33-39 ◽  
Author(s):  
Cristina Velasco ◽  
Marta Librán-Pérez ◽  
Cristina Otero-Rodiño ◽  
Marcos A. López-Patiño ◽  
Jesús M. Míguez ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Lipid Metabolism ◽  
Oncorhynchus Mykiss ◽  
Rainbow Trout Oncorhynchus Mykiss

scholarly journals Effects of Dietary Inclusion of Canthaxanthin- and α-Tocopherol-Loaded Liposomes on Growth and Muscle Pigmentation of Rainbow Trout (Oncorhynchus mykiss)

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Tran Quoc Toan ◽  
Viet Anh Dang ◽  
Quoc Long Pham ◽  
Phi Hung Nguyen ◽  
Thu Huong Trinh ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Fish Muscle ◽  
Fish Growth ◽  
Fish Feed ◽  
Carotenoid Pigment ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Mean Size ◽  
The Difference ◽  
Particle Size Characterization

Dietary inclusion of canthaxanthin, a common carotenoid pigment, has been long practiced in aquaculture to give the favorable flesh color in farmed salmonids. However, carotenoids are associated with limited solubility and poor physicochemical stability, and their dose in fish feed is widely regulated. In this study, we included canthaxanthin- and α-tocopherol-loaded liposomes into fish diets and evaluated the effects of supplemented fish feed on fish growth, color, nutrition, and canthaxanthin deposition in fillets of cultured rainbow trout (Oncorhynchus mykiss). The liposomes were fabricated using lecithin as phospholipids with the initial concentrations (IC = mcanthaxanthin/mlipids, % wt/wt) of canthaxanthin at 0.1%, 0.5%, and 1.0%. Particle size characterization showed that liposome mean sizes were 109.70 ± 6.36, 105.10 ± 8.41, and 109.20 ± 5.66 nm (mean ± SD; n = 3), respectively, corresponding with liposomes synthesized at canthaxanthin IC = 0.1%, IC = 0.5%, and IC = 1%. The polydispersity index (PDI) of all samples remained lower than 0.2. There were no significant differences in the mean size and PDI between blank lecithin liposome and canthaxanthin- and α-tocopherol-loaded liposomes. The encapsulation efficiency of canthaxanthin- and α-tocopherol-loaded liposomes decreased when increasing the concentration of canthaxanthin in lecithin liposomes, with EE% values of IC = 0.1%, IC = 0.5%, and IC = 1% being 85.3 ± 2.1, 72.9 ± 1.8, and 55.3 ± 2.6, respectively. For fish growth, at the end of the experiment, final weight was significantly higher in fish fed with diet supplemented with 1 g/kg canthaxanthin- and α-tocopherol-loaded liposomes (IC = 0.5%) in comparison to other experimental control groups. The difference in color of the salmon muscle was most apparent after two months of feeding. However, after three months, there was no noticeable change in the color score of the fish muscle, indicating saturation of color of the fish muscle. The above results suggest the potential of canthaxanthin- and a-tocopherol-loaded liposomes as the red pigment in fish aquaculture.

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