scholarly journals Particle Size-Dependent Antibacterial Activity and Murine Cell Cytotoxicity Induced by Graphene Oxide Nanomaterials

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Lin Zhao ◽  
Guangxin Duan ◽  
Zaixing Yang ◽  
Jeffrey K. Weber ◽  
Xu Liu ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Morphological Changes ◽  
Graphene Quantum Dots ◽  
Antibacterial Properties ◽  
Antibacterial Activities ◽  
Raw 264.7 Cells ◽  
Cell Cytotoxicity ◽  
Cytotoxic Effects ◽  
Bacterial Colony ◽  
Wide Range

Recent studies have indicated that graphene and its derivative graphene oxide (GO) engage in a wide range of antibacterial activities with limited toxicity to human cells. Here, we systematically evaluate the dependence of GO toxicity on the size of the nanoparticles used in treatments: we compare the cytotoxic effects of graphene quantum dots (GQDs, <15 nm), small GOs (SGOs, 50–200 nm), and large GOs (LGOs, 0.5–3 μm). We synthesize the results of bacterial colony count assays and SEM-based observations of morphological changes to assess the antibacterial properties that these GOs bring into effect againstE. coli. We also use Live/Dead assays and morphological analysis to investigate changes to mammalian (Murine macrophage-like Raw 264.7) cells induced by the presence of the various GO particle types. Our results demonstrate that LGOs, SGOs, and GQDs possess antibacterial activities and cause mammalian cell cytotoxicity at descending levels of potency. Placing our observations in the context of previous simulation results, we suggest that both the lateral size and surface area of GO particles contribute to cytotoxic effects. We hope that the size dependence elucidated here provides a useful schematic for tuning GO-cell interactions in biomedical applications.

Effect of Silver Ions on the Structural and Antibacterial Properties of Bone Replacement Material

2013 ◽  
Vol 795 ◽  
pp. 692-696 ◽  
Author(s):  
Nida Iqbal ◽  
Mohammed Rafiq Abdul Kadir ◽  
Nasrul Humaimi Bin Mahmood ◽  
Micheal Moses ◽  
Mashitah Binti Mad Salim ◽  
...  
Keyword(s):  
Calcium Phosphates ◽  
Bacterial Species ◽  
Calcium Nitrate ◽  
Antibacterial Properties ◽  
Silver Ions ◽  
Antibacterial Activities ◽  
Silver Addition ◽  
Wide Range ◽  
Microbial Infections ◽  
Inorganic Mineral

Antibacterial materials based on calcium phosphates have wide range of biomedical applications in the prevention of microbial infections. The synthesis of inorganic mineral component of bone i.e. hydroxyapatite was done with the addition of silver (Ag) (5-15 wt %) as antibacterial agent. The wet precipitation synthesis was carried out using diammonium hydrogen phosphate and calcium nitrate as P and Ca precursors. The presence and effect of silver addition on the structure was studied using Fourier Transform-Infrared (FTIR) spectroscopy and Energy Dispersive X-ray (EDX) techniques. The antibacterial properties of all samples were evaluated using Disc Diffusion Technique (DDT) againstS. aureus,B. subtilis, P. aeruginosaandE. coli. Antibacterial activities of samples were found to vary depending on the bacterial species and Ag loading percentage. The antibacterial assay suggested that the addition of Ag ions within hydroxyapatite can be effectively provided the required level of antibacterial activity against bacteria.

Synthesis, Photoluminescence and Bio-Targeting Applications of Blue Graphene Quantum Dots

2016 ◽  
Vol 16 (4) ◽  
pp. 3457-3467 ◽  
Author(s):  
Jigang Wang ◽  
Ji Zhou ◽  
Wenhua Zhou ◽  
Jilong Shi ◽  
Lun Ma ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Graphene Oxide ◽  
Sodium Hydroxide ◽  
Heating Temperature ◽  
Thin Sheet ◽  
Graphene Quantum Dots ◽  
Photoluminescence Intensity ◽  
Cytotoxic Effects ◽  
Different Temperatures ◽  
Blue Photoluminescence

Chemical derived graphene oxide, an atomically thin sheet of graphite with two-dimensional construction, offers interesting physical, electronic, thermal, chemical, and mechanical properties that are currently being explored for advanced physics electronics, membranes, and composites. Herein, we study graphene quantum dots (GQD) with the blue photoluminescence under various parameters. The GQD samples were prepared at different temperatures, and the blue photoluminescence intensity of the solution improved radically as the heating temperatures increased. Concerning PL peak and intensity of the quantum dots, the results demonstrated dependence on time under heating, temperature of heating, and pH adjusted by the addition of sodium hydroxide. After hydrothermal synthesis routes, the functional groups of graphene oxide were altered the morphology showed the stacking configuration, and self-assembled structure of the graphene sheets with obvious wrinkles appeared at the edge structures. In addition, absorption, PL, and PLE spectra of the graphene quantum dots increase with different quantities of sodium hydroxide added. Finally, using GQD to target PNTIA cells was carried out successfully. High uptake efficiency and no cytotoxic effects indicate graphene quantum dots can be suitable for bio-targeting.

scholarly journals Graphene Oxide Reinforced Polycarbonate Nanocomposite Films with Antibacterial Properties

2016 ◽  
Vol 2016 ◽  
pp. 1-10 ◽  
Author(s):  
R. Mahendran ◽  
D. Sridharan ◽  
K. Santhakumar ◽  
T. A. Selvakumar ◽  
P. Rajasekar ◽  
...  
Keyword(s):  
Thin Films ◽  
Graphene Oxide ◽  
Low Cost ◽  
Thermal Studies ◽  
Composite Films ◽  
Antibacterial Properties ◽  
Antibacterial Activities ◽  
Nanocomposite Films ◽  
Matrix Microstructure ◽  
Phase Inversion Technique

The incorporation of carbonaceous nanofillers into polymers can result in significant materials with improved physicochemical properties and novel composite functionalities. In this study, we have fabricated antibacterial, lightweight, transparent, and flexible graphene oxide (GO) reinforced polycarbonate thin films by a facile and low-cost methodology. Solution blending is employed to get a homogeneous mixture of PC-GO composites at various loading of GO, and the thin films are prepared by dry-wet phase inversion technique. Thermal studies and micrographs of the films revealed the incorporation of GO in PC matrix. Microstructure of the thin films showed the homogeneous dispersion of GO at micro- and nanoscales; however, at higher loading of GO (0.7%), significant agglomeration is observed. More importantly, PC-GO composite films exhibited excellent antibacterial activities against E. coli and S. aureus, owing to the antibacterial nature of GO nanoparticles.

scholarly journals Synthesis, Characterization, Photo-Luminescence and Antibacterial Activities of Silica-Graphene Oxide Composites

2021 ◽  
Author(s):  
Imad H. Khaleel ◽  
Abdulkareem A. Alkhafaji ◽  
Hussein A. Miran ◽  
Zainab N. Jaf
Keyword(s):  
Thin Film ◽  
Graphene Oxide ◽  
Sol Gel ◽  
Antibacterial Properties ◽  
Dye Adsorption ◽  
Photo Luminescence ◽  
Antibacterial Activities ◽  
Nano Particles ◽  
Glass Substrates ◽  
Nano Powder

In this research, a novel thin film Si-GO10 and nano-powders Si-GO30 of silica-graphene oxide composite were prepared via sol-gel method and deposited on glass substrates using spray pyrolysis. XRD results showed a relatively strong peak in graphite layer that corresponds to the plane of (002). TEM images displayed that SiO<sub>2</sub> nano-particles were randomly distributed on the surface of Si-GO30 sample, and the particle size in these nano-powders was below 50 nm. Moreover, silica nano-particles on the surface of GO plates exhibited almost a spherical and rod-like nanoparticles shapes, which in turn confirmed the formation of SiO<sub>2</sub>-GO nano-hybrids. FESEM analysis reveals a different morphology, the Si-GO10 sample is so rough and crumble, while the Si-GO30 sample is relatively smooth on the surface. Photocatalytic investigations revealed the composite materials exhibit high activity for dye adsorption and decomposition. Si-GO10 thin film did not undergo degradation after 120 minutes, however, for Si-GO30 nano-powder, the adsorption peak intensity was reduced to 665 nm, indicating a decrease in the dye concentration in the solution. FTIR analysis demonstrated that carboxylic functional groups are decreased by increasing silica particles. Photo-Luminescence (PL) spectrum in Si-GO10 thin film disclosed a severe emission peak at about 675 nm. This spectrum was completely disappeared in Si-GO30 nano-powders. Results of the antibacterial properties emphasized that Si-GO30 nano-particle would prevent Escherichia. coli growth after 20 hours. The presented methodology allows for the synthesis of graphene oxide supported silicon dioxide nano-particles for promising applications in photocatalytic and antibacterial fields.

scholarly journals Analysis of the Chemical Profiles and Anti-S. aureus Activities of Essential Oils Extracted from Different Parts of Three Oregano Cultivars

Foods ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2328
Author(s):  
Yuanpeng Hao ◽  
Jingyi Li ◽  
Wenying Zhang ◽  
Meiyu Sun ◽  
Hui Li ◽  
...  
Keyword(s):  
Essential Oils ◽  
Food Industry ◽  
Antibacterial Properties ◽  
Antibacterial Activities ◽  
Chemical Compositions ◽  
High Antibacterial Activity ◽  
Wide Range ◽  
Tissue Factors ◽  
Chemical Profiles ◽  
Different Parts

The use of antibiotics in the food industry is highly regulated owing to the potential harmful effects of antibiotics on human health. Therefore, it is crucial to seek alternatives for ensuring food safety. Essential oils (EOs) extracted from plants of the genus Origanum exhibit a wide range of chemical and antibacterial activities. Species and tissue factors shape the production and accumulation processes of EOs in Origanum plants, thereby affecting their bactericidal activity. In this study, the morphologies and EO yields from the inflorescences, leaves, and stems of three oregano cultivars were evaluated. In addition, the chemical compositions and antibacterial abilities of oregano EOs (OEOs) were assessed. The results showed that OEOs from the different parts of the plant displayed only minor differences in chemical composition, whereas the yield of EOs varied considerably. Additionally, the chemical profiles of OEOs differed significantly among cultivars. The carvacrol content in the OEOs was closely related to its activity against Staphylococcus aureus; the antibacterial properties of the OEOs were further verified using carvacrol. These findings suggested that OEOs possessing high antibacterial activity may have the potential to be developed as bactericides in the food industry.

Antibacterial activities and mechanisms of fluorinated graphene and guanidine-modified graphene

RSC Advances ◽  
2016 ◽  
Vol 6 (11) ◽  
pp. 8763-8772 ◽  
Author(s):  
Xu Wang ◽  
Peng Lu ◽  
Yuan Li ◽  
Huining Xiao ◽  
Xiangyang Liu
Keyword(s):  
Graphene Oxide ◽  
Antibacterial Properties ◽  
Antibacterial Activities ◽  
Graphene Derivatives ◽  
Fluorinated Graphene ◽  
Modified Graphene

The antibacterial properties and mechanism of three types of graphene derivatives, graphene oxide (GO), fluorinated graphene (FG), and guanidine-modified graphene (PHGH-G), were comparatively studied.

scholarly journals Antibacterial Performance of a Mussel-Inspired Polydopamine-Treated Ag/Graphene Nanocomposite Material

Materials ◽  
2019 ◽  
Vol 12 (20) ◽  
pp. 3360
Author(s):  
Jianming Liao ◽  
Shuaiming He ◽  
Shasha Guo ◽  
Pengcheng Luan ◽  
Lihuan Mo ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Antibacterial Properties ◽  
Great Promise ◽  
X Ray ◽  
Antibacterial Performance ◽  
Reduced Graphene ◽  
Wide Range ◽  
Graphene Nanocomposite

Graphene-based nanocomposites have attracted tremendous attention in recent years. In this study, a facile yet effective approach was developed to synthesize reduced graphene oxide and an Ag–graphene nanocomposite. The basic strategy involved in the preparation of reduced graphene oxide includes reducing graphene oxide with dopamine, followed by in situ syntheses of the Ag-PDA-reducing graphene oxide (RGO) nanocomposite through adding AgNO3 solution and a small amount of dopamine. The nanocomposite was characterized by transmission electron microscopy (TEM), atomic force microscopy (AFM), X-ray diffraction (XRD), FTIR spectra, Raman spectra, ultraviolet-visible (UV-vis), and X-ray photoelectron spectroscopy (XPS), results indicated that a uniform PDA film is formed on the surface of the GO and GO is successfully reduced. Besides, the in situ synthesized Ag nanoparticles (AgNPs) were evenly distributed on the RGO surface. To investigate antibacterial properties Ag-PDA-RGO, different dosages were selected for evaluating the antibacterial activity against Gram-positive bacteria Staphylococcus aureus and Gram-negative bacteria Escherichia coli. The Ag-PDA-RGO nanocomposites displayed excellent antibacterial property. The antibacterial ratio reached 99.9% against S. aureus and 90.9% against E. coli when the dosage of 100 mg/L Ag-PDA-RGO nanocomposites was 100 μL. The novel Ag-PDA-RGO nanocomposite prepared by a facile yet effective, environmentally friendly, and low-cost method holds great promise in a wide range of modern biomedical applications.

Enhanced catalytic and antibacterial activities of silver nanoparticles immobilized on poly(N-vinyl pyrrolidone)-grafted graphene oxide

RSC Advances ◽  
2015 ◽  
Vol 5 (100) ◽  
pp. 81994-82004 ◽  
Author(s):  
Shikha Singh ◽  
Ravi Kumar Gundampati ◽  
Kheyanath Mitra ◽  
K. Ramesh ◽  
Medicherla V. Jagannadham ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Graphene Oxide ◽  
Ag Nanoparticles ◽  
Antibacterial Properties ◽  
Antibacterial Activities ◽  
Vinyl Pyrrolidone

PNVP grafted on GO regulates the size of Ag nanoparticles and enhances the catalytic and antibacterial properties along with increase in the dispersibility of GO.

scholarly journals Graphene Oxide in a Composite with Silver Nanoparticles Reduces the Fibroblast and Endothelial Cell Cytotoxicity of an Antibacterial Nanoplatform

2019 ◽  
Vol 14 (1) ◽  
Author(s):  
Mateusz Wierzbicki ◽  
Sławomir Jaworski ◽  
Ewa Sawosz ◽  
Anna Jung ◽  
Grzegorz Gielerak ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Graphene Oxide ◽  
Chicken Embryo ◽  
Salmonella Enteritidis ◽  
Antimicrobial Agents ◽  
Human Fibroblasts ◽  
Antibacterial Properties ◽  
Chorioallantoic Membrane ◽  
Cell Cytotoxicity ◽  
Oxide Composite

Abstract Antibacterial surfaces coated with nanomaterials, including silver nanoparticles, are considered effective alternative antimicrobial agents that can be used instead of antibiotics and chemical agents. However, reports of the potential toxicity of these materials raise questions about the safety of their use in biomedical applications. The objective of this research was to reduce the human cell cytotoxicity of silver nanoparticle-coated polyurethane foils by complexing silver nanoparticles with graphene oxide. The antimicrobial activity of nanoplatforms coated with silver nanoparticles, graphene oxide and the composite of silver nanoparticles and graphene oxide was assessed with Salmonella enteritidis. Cytotoxicity was analysed by an analysis of the viability and morphology of human fibroblasts, human umbilical vein endothelial cells (HUVECs) and chicken embryo chorioallantoic membrane. Additionally, the synthesis level of inflammatory proteins was examined for fibroblasts cultured on different nanoplatforms. The nanoplatform coated with the silver nanoparticles and graphene oxide composite showed strongest antibacterial properties, although nanoplatforms coated with only silver nanoparticles or graphene oxide also resulted in decreased S. enteritidis growth. Furthermore, a nanoplatform coated with silver nanoparticles and graphene oxide composite showed limited immunological stimulation and significantly reduced cytotoxicity towards fibroblasts, HUVECs and chicken embryo chorioallantoic membrane in comparison to the nanoplatform coated only with silver nanoparticles, due to the higher stability of the nanomaterials in the nanocomposite.

scholarly journals Reduction of graphene oxide and graphene quantum dots using nascent hydrogen: The investigation of morphological and structural changes

2020 ◽  
Vol 5 (1) ◽  
pp. 1-4
Author(s):  
Svetlana Jovanovic ◽  
Olaf C. Haenssler ◽  
Milica Budimir ◽  
Duška Kleut ◽  
Jovana Prekodravac ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Graphene Oxide ◽  
Chemical Composition ◽  
Electrical Properties ◽  
Chemical Modification ◽  
Structural Changes ◽  
Morphological Changes ◽  
Graphene Quantum Dots ◽  
Structure And Morphology

AbstractIn order to modify both chemical and electrical properties of graphene-based nanomaterials, we conducted the chemical modification of graphene oxide (GO) and graphene quantum dots (GQDs). The reaction of the reduction with nascent hydrogen was conducted on both materials. The structure and morphology of produced chemically reduced GO and GQDs were analyzed. While the chemical composition of both GQD and GO changed significantly, GO showed also significant changes in morphology as opposite to GQDs where were morphological changes were not observed.

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