scholarly journals Analysis of Polyphenolic Compounds in Extracts from Leaves of SomeMalus domesticaCultivars: Antiradical and Antimicrobial Analysis of These Extracts

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Alina Sowa ◽  
Grażyna Zgórka ◽  
Aleksandra Szykuła ◽  
Roman Franiczek ◽  
Beata Żbikowska ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Ethyl Acetate ◽  
Candida Glabrata ◽  
Antiradical Activity ◽  
Colorimetric Method ◽  
Water Extract ◽  
Diffusion Method ◽  
Strong Activity ◽  
Ethyl Acetate Extracts

In this study, methanol, ethyl acetate, water extracts, and precipitate were obtained from leaves ofMalus domesticacultivars: Golden delicious, Jonagold, Elstar, Ligol, and Mutsu. Antiradical activity of these extracts was measured using theABTS+∙radical, and antimicrobial activity was measured with the disk-diffusion method. Phenolic compounds were measured with the colorimetric method and identified with high performance liquid chromatography (HPLC). The highest antiradical activity was observed for the Jonagold variety, and in particular strong activity was noted for ethyl acetate extracts. Antimicrobial activity was observed against strains ofStaphylococcus aureus,Enterococcus faecalis, and the fungusCandida glabrata. Particularly susceptible to the extracts activity appeared to beStaphylococcus aureus, but the growth ofCandida glabratawas inhibited in the presence of ethyl acetate extracts. With the HPLC method we identified a high amount of phloridzin (above 500 mg per g of ethyl acetate extracts), lower amounts of hyperoside, isoquercitrin, and quercitrin, and traces ofp-hydroxybenzoic and chlorogenic acids. The contribution of phloridzin to antiradical activity of methanol and ethyl acetate extracts was very high (above 90%). In water extract the contribution of phloridzin was between 38.9 and 55.2%, chlorogenic acid 22.7 and 36.1%, and hyperoside 12.2 and 13.3%.

scholarly journals BIOAKTIVITAS JAMUR Aspergillus flavus YANG BERSIMBION DENGAN ASCIDIAN Eudistoma sp.

2020 ◽  
Vol 8 (2) ◽  
pp. 11
Author(s):  
Alfa Mongi ◽  
Deiske A Sumilat ◽  
Fitje Losung ◽  
Remmy E P Mangindaan ◽  
Rosita A Lintang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Candida Albicans ◽  
Ethyl Acetate ◽  
Aspergillus Flavus ◽  
Marine Invertebrate ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Antibacterial And Antifungal ◽  
Ethyl Acetate Extracts

Ascidian is a marine invertebrate that produce bioactive compounds such as antibacterial and antifungal. The purpose of this study was to obtain symbiotic fungi isolates, ethyl acetate extracts from ascidian symbiotic isolates, and to determine the antibacterial and antifungal activities of ethyl acetate extracts of symbiotic isolates using the diffusion method (disc diffusion Kirby and Bauer) against Escherichia coli, Staphylococcus aureus bacteria and against the Candida albicans fungus. The results obtained 1 ascidian species which was cultured to obtain pure isolates, the pure isolates were tested molecularly and the results showed that the isolate was Aspergillus flavus, then the isolates were grown on rice medium and extracted. The results of the fungal extract showed the presence of antibacterial and antifungal activity against Escherichia coli by 10 mm, Staphylococcus aureus by 19 mm and against the Candida albicans by 13.5 mm. Keywords : Ascidian, Eudistoma sp., Aspergillus flavus, Antibacterial, Antifungal, Symbiotic.                                                  AbstrakAscidian merupakan avertebrata laut yang dapat menghasilkan senyawa bioaktif yang seperti antibakteri dan antijamur. Tujuan dari penelitian ini yaitu mendapatkan isolat jamur simbion dari ascidian, mendapatkan ekstrak etil asetat dari isolat jamur simbion ascidian, dan menguji aktivitas antibakteri dan antijamur ekstrak etil asetat isolat jamur simbion dengan metode difusi agar (disc diffusion Kirby and Bauer) terhadap bakteri Escherichia coli, Staphylococcus aureus dan terhadap jamur Candida albicsans. Hasil penelitian didapatkan 1 jenis ascidian yang kemudian dikultur untuk mendapatkan isolat murni, isolat murni diuji secara molekuler dan didapati hasil bahwa isolat tersebut merupakan jamur Aspergillus flavus. Isolat tersebut kemudian ditumbuhkan pada media nasi dan diekstrak. Hasil ekstrak jamur tersebut menunjukkan adanya aktivitas antibakteri dan antijamur terhadap bakteri Escherichia coli sebesar 10 mm, Staphylococcus aureus sebesar 19 mm dan terhadap jamur Candida albicsans 13,5 mm. Kata kunci : Ascidian, Eudistoma sp., Aspergillus flavus, Antibakteri, Antijamur, Simbion.

scholarly journals Screening of antimicrobial activity of aqueous and ethanolic extracts of some medicinal plants from Cameroon and assessment of their synergy with common antibiotics against multidrug-resistant uropathogenic bacteria.

2021 ◽  
Author(s):  
Mbarga Manga Joseph Arsene ◽  
Podoprigora Irina Viktorovna ◽  
Anytoulou Kitio Linda Davares ◽  
Mouafo Hippolyte Tene ◽  
Irma Aurelia Monique Manga ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Klebsiella Oxytoca ◽  
Diffusion Method ◽  
Distilled Water ◽  
Strong Activity ◽  
E Coli ◽  
Ethanolic Extracts ◽  
Uropathogenic Bacteria

Background and aim: The Cameroonian flora abounds in plants with multidimensional therapeutic virtues which can play an important role in the fight against resistance to antibiotics and the search for new antimicrobials. This study aimed to screen the antimicrobial potential of aqueous and ethanolic extracts of thirteen (13) samples (Bark, leaf, seed) of eight (8) plants from Cameroon against 3 reference pathogens and to evaluate their synergy with conventional antibiotics against eleven (11) multiresistant uropathogenic (MRU) bacteria. Method: Bioactive compounds were extracted from leaves of Leucanthemum vulgare, Cymbopogon citratus (DC.) Stapf, Moringa oleifera Lam and Vernonia amygdalina Delile; barks of Cinchona officinalis and Enantia chlorantha Oliv; barks and seeds of Garcinia lucida Vesque and leaves and seeds of Azadirachta indica (Neem) using distilled water and ethanol as solvents. The extracts were tested against Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 6538 and Candida albicans 10231 using the well diffusion method and the microdilution method. The synergistic effect was assessed (using disc diffusion method and the checkerboard method) against MRU bacteria namely Achromobacter xylosoxidans 4892, Citrobacter freundii 426, Enterococcus avium 1669, Escherichia coli 1449, Klebsiella oxytoca 3003, Kocuria rizophilia. 1542, Moraxella catarrhalis 4222, Morganella morganii 1543, Pseudomonas aeruginosa 3057, Staphylococcus aureus 1449 and Streptococcus agalactiae 3984. Results: We found that distilled water extracted a more important mass of phytochemical compounds (7.9-21.2%) compared to ethanol (5.8-12.4%). Except C. officinalis and G. lucida leaves, the rest of extracts were active with inhibition diameters (ID) ranging from 5 to 36 mm. Both ethanolic (EE) and aqueous extract (AE) of E. chloranta bark (ECB) were the most active against all pathogens with the mean ID of 17 and 36 mm vs S. aureus ATCC 6538, 23 and 14 mm vs E. coli ATCC 25922 and 36 and 19 mm vs C. albicans ATCC 10231. Only the EE of E. chloranta bark (ECB) had a strong activity against all the microorganisms tested (MIC <2 mg / ml); L. vulgare leaves (LVL) and G. lucida seed (GLS) had moderate (average MIC of 8 mg/ml) while all other extracts showed very weak antimicrobial activity. In addition, the fractional inhibitory concentration (FIC) ranged from 0.125 to 0.750. No antagonism (FIC> 4) or indifference (1≤ FIC≤4) was noted between the extracts and the antibiotics, but the best synergies were found with ECB which well-modulated Kanamycin (FIC = 0.125 against S. aureus and 0.250 against E. coli), nitrofurantoin (FIC = 0.250 against S. aureus and 0.188 against E. coli) and ampicillin (FIC = 0.125 against E. coli). Similarly, compared to other extracts, ECB, LVL and GLS also well-modulated ampicillin, ceftazidime, tetracycline, nitrofurantoin, and trimethoprim against all the above-mentioned resistant uropathogenic bacteria with important increase in fold area (IFA). Conclusion: This study show that E. chlorantha bark, L. vulgare leaves G. lucida seed, have good antimicrobial activity against both bacteria (Gram positive and Gram negative) and fungi (C. albicans); and should be more investigated for their possible use to the fight against MDR and MRU microorganisms.

scholarly journals Phytochemical Screening and in vitro Antimicrobial Activity of Cyperus rotundus Leaf Extracts

2021 ◽  
Vol 4 (1) ◽  
pp. 43-52
Author(s):  
EO Agbo ◽  
JO Ogenyi ◽  
CA Adah ◽  
CT Agber
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Ethyl Acetate ◽  
Plant Extracts ◽  
Methanol Extract ◽  
Cyperus Rotundus ◽  
Diffusion Method ◽  
Methanol Extracts ◽  
Study Results

Cyperus rotundus, a member of family Cyperaceae is a perennial, stoloniferous herb. The study investigated the secondary metabolites present in the leaf of Cyperus rotundus and antimicrobial activities of the hexane, ethyl acetate and methanol extracts. Phytochemical analysis revealed the presence of saponins, alkaloids, phenolic compounds, tannins, steroids, flavonoids, cardiac glycosides and terpenoids. The qualitative test of the extracts show the presence of steroid only in the hexane extract; flavonoid, cardiac glycoside in both the ethyl acetate and methanol extracts; saponin and tannin and phenolics in just the methanol extract. While alkaloid was present in all the extracts, terpenoid was absent in all. Antimicrobial activity of plant extracts were monitored using the agar disk-diffusion method against strains gram-positive bacterial: Staphylococcus aureus, Pseudomonas aeruginosa; and gram-negative bacteria: Escherichia coli, Salmonela typhi, and strains of fungi: Candida albicans and Aspergillus niger. The antimicrobial activity of the extracts was monitored at different concentrations of 50, 25, 12.5 and 6.25 mg/mL prepared from the stock concentration (100mg/mL) by serial dilution; with standard drugs: ciprofloxacin and fluconazole. The inhibition of the test organisms were concentration dependent. The methanol extract showed significantly higher Inhibition Zone (IZ) and Activity Index (AI) against the microbes at all concentrations compared to the ethyl acetate and hexane extracts due to the much phytochemicals (saponins, tanins, phenols, flavonoids, cardiac glycocides and alkaloids) found present in its extract. The overall study results signify the potential of Cyperus rotundus as a source of therapeutic agents. The methanol extract showed the best activity against both bacterial and fungal pathogens at the highest concentration of 100 mg/mL, especially the most susceptible bacteria: Staphylococcus aureus (IZ of 25.0±1.5; AI of 0.96) and Candida albicans (IZ of 25.5±0.5; AI of 0.91). At MIC, MBC and MFC of 6.25 mg/mL and 12.5 mg/mL, the plant extracts showed effective bactericidal and fungicidal effect against the test microbes.

scholarly journals <i>In-vitro</i> antibacterial sensitivity of <i>Usnea barbata</i> lichen extracted with methanol and ethyl-acetate against selected <i>Staphylococcus</i> species from milk of cows with mastitis

2014 ◽  
Vol 57 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Emrobowansan Monday Idamokoro ◽  
Patrick Julius Masika ◽  
Voster Muchenje ◽  
Daniel Falta ◽  
Ezekiel Green
Keyword(s):  
Antimicrobial Activity ◽  
Medicinal Plant ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Bovine Milk ◽  
Raw Milk ◽  
Diffusion Method ◽  
Biochemical Tests ◽  
Ethyl Acetate Extracts

Abstract. This study aimed at evaluating the antimicrobial potential of Usnea barbata lichen as a medicinal plant against selected Staphylococcus species isolated from raw milk of cows. In-vitro screening of methanol and ethyl-acetate extracts from Usnea barbata lichen were evaluated to determine their antimicrobial activity against thirteen different Staphylococcus species. The selected organisms were isolated from raw bovine milk and identified using several biochemical tests and confirmed with API staph kit. The antimicrobial activity of the extracts were evaluated using both the agar well diffusion method (at 5 mg/ml, 10 mg/ml and 20 mg/ml) and the broth micro-dilution technique to determine the mean zone of inhibition and the minimum inhibitory concentration (MIC), respectively. Both the methanol and ethyl-acetate extracts showed variable antimicrobial activity against the Staphylococcus species with mean zones of inhibition ranging from 0-34 mm in diameter at 5 mg/ml, 10 mg/ml and 20 mg/ml, respectively. Susceptibility by the Staphylococcus species tested in the methanol and the ethyl-acetate extract was 92.31 % and 53.85 %, respectively. The MIC result for the methanol extract ranged from 0.04 to 10 mg/ml, while that of the ethyl-acetate extract ranged from 0.16 to 5 mg/ml. Results from this study revealed the in vitro microbial activity of Usnea barbata extracts which indicate its potential as a medicinal plant.

IDENTIFICATION AND CHARACTERIZATION OF POTENT ANTIMICROBIAL SECONDARY METABOLITE GENERATED FROM ENDOPHYTIC FUNGI ISOLATED FROM JAMBLANG PLANT ( Eugenia cumini L)

2017 ◽  
Vol 10 (4) ◽  
pp. 283
Author(s):  
Shirly Kumala ◽  
Ng Vini Aprilia ◽  
Partomuan Simanjuntak
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Fatty Acid ◽  
Secondary Metabolites ◽  
Ethyl Acetate ◽  
Endophytic Fungi ◽  
Large Scale ◽  
Diffusion Method

Objective: Colletotrichium capsici endophytic fungi isolated from Jamblang plant (Eugenia cumini  L).  The secondary metabolites of this plant has the potent antibacterial efficacy  as well as diarrheal and anti-diabetic. This research focussed on isolation of the endophytic microbes from branches of Jamblang plants and their secondary metabolites.Methods: Isolation of endophytes were performed in PDA(potato dextrose agar) using direct seed plant. Endophytic fungi isolates with strongest antimicrobial activity against the bacteria Staphylococcus aureus, Escherichia coli were fermented in Potato Dextrose Yeast (PDY) to produce  large scale of the metabolites.  Supernatant was extracted with ethyl acetate solvent. Ethyl acetate extract fractionated by column chromatography (SiO3, n-hexane- ethyl acetate = 50:1 ~ 1:1) and obtained three fractions. Further, agar diffusion method was performed to assess their anti-microbial activity.Results:  Antibacterial  test  results  indicated that fraction III had  the antibacterial  activity Staphylococcus aureus with  inhibition zone diameter  of 10.7 mm but no observed antibacterial activity against Escherichia coli.  Furthermore, identification  by GC-MS showed that compounds present in fraction III was mainly fatty acid and phenolic compounds.Conclusion:  In conclusion, secondary metabolites isolated from Jamblang plants branches contained predominantly fatty acid and phenol related compounds that could be responsible for its potent anti microbial activity. Keywords : Endophytic fungi, Jamblang (Eugenia cumini L.), antimicrobial activity

scholarly journals Antimicrobial activity and bioautographic study of antistaphylococcal components from Caesalpinia pyramidalis Tull.

2012 ◽  
Vol 48 (1) ◽  
pp. 147-154 ◽  
Author(s):  
Antonio Marcos Saraiva ◽  
Cristiane Lopes Saraiva ◽  
Admário Marques Gonçalves ◽  
Rogério Ribeiro Soares ◽  
Fabrício de Oliveira Mendes ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Ethyl Acetate ◽  
Antimicrobial Agents ◽  
Inhibitory Concentration ◽  
Methanol Extract ◽  
Diffusion Method ◽  
Phytochemical Study ◽  
Multiresistant Strains ◽  
Active Substances ◽  
Ethyl Acetate Extracts

The antimicrobial activity of dry methanol and ethyl acetate extracts for the leaves, bark of the stem, peel of the root, flower, fruit and seed of Caesalpinia pyramidalis Tull. (catingueira) was performed against seventeen isolates of Staphylococcus aureus MRSA multiresistant strains, which included two isolates of S. aureus MSSA and two ATCC strains. The antimicrobial activity was tested by the agar diffusion method and the Minimum Inhibitory Concentration (MIC) was determined. The dry methanol extract of the root showed good antimicrobial activity with a MIC of less than 0.5 mg.mL-1. The dry ethyl acetate extracts exhibited lower antimicrobial activity, which might be explained by solubility problems and less diffusion in the agar medium. Results of the bioautographies also confirmed inhibition halos corresponding to the active substances present in the leaves, as well as in the flower of C. pyramidalis. The phytochemical study of the leaves, bark of the stem, peel of the root, flower and fruit of extracts from C. pyramidalis confirmed the presence of a number of known antimicrobial agents including ursolic acid, quercetin, catechin, ellagic acid, sitosterol, flavonoids, proanthocyanidins and gallic acid.

scholarly journals Antimicrobial Activity of Divaricatic Acid Isolated from the Lichen Evernia mesomorpha against Methicillin-Resistant Staphylococcus aureus

Molecules ◽  
2018 ◽  
Vol 23 (12) ◽  
pp. 3068 ◽  
Author(s):  
Jong Oh ◽  
Yi Kim ◽  
Hyo-Seung Gang ◽  
Jin Han ◽  
Hyung-Ho Ha ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Staphylococcus Epidermidis ◽  
Enterococcus Faecium ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Inhibitory Concentration ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Acetone Extracts ◽  
Ethyl Acetate Extracts

One hundred and seventy seven acetone extracts of lichen and 258 ethyl acetate extracts of cultured lichen-forming fungi (LFF) were screened for antimicrobial activity against Staphylococcus aureus and Enterococcus faecium using a disk diffusion method. Divaricatic acid was isolated from Evernia mesomorpha and identified by LC-MS, 1H-, 13C- and DEPT-NMR. Purified divaricatic acid was effective against Gram + bacteria, such as Bacillus subtilis, Staphylococcus epidermidis, Streptococcus mutans, and Enterococcus faecium, with the minimum inhibitory concentration (MIC) values ranging from 7.0 to 64.0 μg/mL, whereas vancomycin was effective in the MICs ranging from 0.78 to 25.0 μg/mL. Interestingly, the antibacterial activity of divaricatic acid was higher than vancomycin against S. epidermidis and E. faecium, and divaricatic acid was active against Candida albicans. In addition, divaricatic acid was active as vancomycin against S. aureus (3A048; an MRSA). These results suggested that divaricatic acid is a potential antimicrobial agent for the treatment of MRSA infections.

scholarly journals AKTIVITAS ANTIBAKTERI EKSTRAK KULIT PISANG KEPOK KUNING (Musa paradisiaca L.) TERHADAP BAKTERI Staphylococcus aureus DAN Escherichia coli SERTA PENENTUAN TOTAL FLAVONOID DAN FENOL DALAM FRAKSI AKTIF

Jurnal Kimia ◽  
2019 ◽  
Vol 13 (1) ◽  
pp. 9
Author(s):  
N. K. D. M.S. Wahyuni ◽  
W. S. Rita ◽  
I. A. R. A. Asih
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Ethyl Acetate ◽  
Ethanol Extract ◽  
Hexane Extract ◽  
Total Flavonoid ◽  
Diffusion Method ◽  
Strong Activity ◽  
Butanol Extract ◽  
Musa Paradisiaca

Peel of yellow kepok banana (Musa paradisiaca L). has not been used optimally, while the peel can be used as an infection medicine The aim of this study was to reveal the activity of kepok yellow banana peel extract against Staphylococcus aureus and Escherichia coli and to determine the total content of flavonoids and phenols in active extract.. Extraction peel of yellow kepok banana was done by maceration and partition method, anti bacterial activity was assayed by wells diffusion method, determination total flavonoid and phenolic contents was done by UV-Vis Spectrophotometer. Maceration of 1 kg peel of yellow banana produced 80.9173 g of crude ethanol extract. The partition of 20 g crude ethanol extract produced 1,3758 g of n-hexane extract, 3,5818 g of ethyl acetate extract, and 1,0762 g of n-butanol extract. Anti bacterial test result showed that the 10% n-butanol extract was active towards S.aureus and E.coli with strong activity compared with ethanol, ethyl acetate, and n-hexane extract. MIC value was 0.5% for S.aureus and 0,2% for E.coli bacteria. The contain total flavonoid and phenol in n-butanol extract respectively were 0.06% and 0.15%.

COMPARATIVE EVALUATION OF IN VITRO ANTIMICROBIAL AND IN VIVO WOUND HEALING ACTIVITY OF ETHANOLIC AND ETHYL ACETATE EXTRACTS OF ABUTILON INDICUM ROOT

INDIAN DRUGS ◽  
2019 ◽  
Vol 56 (04) ◽  
pp. 21-31
Author(s):  
K Ravishankar ◽  
G.V.N Kiranmayi ◽  
J. M. Sruthi ◽  
Keyword(s):  
Antimicrobial Activity ◽  
Wound Healing ◽  
Ethyl Acetate ◽  
Ethanolic Extract ◽  
Diffusion Method ◽  
Wound Healing Activity ◽  
Ethyl Acetate Extracts ◽  
Abutilon Indicum

The present study was aimed at comparing the in vivo wound healing and in vitro antibacterial activity of Abutilon indicum roots. Wound healing is a complex phenomenon which consists of three stages inflammatory, proliferative, and remodelling. Traditionally, A. indicum is used for wound healing. There is no specific scientific data available for the wound healing activity of Abutilon indicum root. The present study was designed to investigate the same. The wound healing activity of ethanolic and ethyl acetate extracts of A. indicum was evaluated in incision and burn wound models. The parameters studied include tensile strength, epithelialisation period and rate of wound contraction. A. indicum also possesses antimicrobial activity. Ethyl acetate and ethanolic extract of A. indicum was tested against both Gram positive, Gram negative and fungal organisms using agar well diffusion method. From the results, it was concluded that ethyl acetate extract of A. indicum root had greater wound activity and antimicrobial activity than the ethanolic extract.

scholarly journals Antimicrobial and in vitro cytotoxic studies of Acampe praemorsa and Aeridis odarata of Orchidaceae

2018 ◽  
Vol 7 (2) ◽  
pp. 2088
Author(s):  
Jhansi Katta ◽  
Khasim S.M.
Keyword(s):  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Eastern Ghats ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Mcf 7 ◽  
Ethyl Acetate Extracts

This paper aims at studying antimicrobial efficacy and in vitro cytoxic activity of epiphytic orchids namely Acampe praemorsa, Aeridis odorata distributed in Eastern Ghats of Visakhapatnam district. Plants were collected, identified following literature, shade dried and Methanol, ethyl acetate extracts were prepared for the systematic investigation of antimicrobial activity of plant extracts. Antibacterial activity against three gram positive bacteria Bacillus megaterium, Lactobacillus acidophillus and Enterococcus faecalis, three gram negative bacteria Proteus vulgaris, Klebsiella pneumoniae and Escherichia coli was done using Agar well diffusion method, antifungal activity was carried against Candida albicans, Aspergillus flavus by Cpzapak dox agar media, MIC by broth dilution method, zones of inhibition were recorded. Ethyl acetate extracts showed maximum antimicrobial activity against all bacteria and fungi, Aeridis odarata ethyl acetate extract showed highest zone of inhibition 17mm against Lactobacillus acidophilus in bacteria, Ethyl acetate extract of Acampe praemorsa showed highest zone of inhibition 17mm against Candida albicans in fungi, the leaf extracts were tested for its inhibitory effect on HeLa and MCF-7 cell lines were evaluated by the MTT assay and methanolic extract of Aeridis odarata has significant cytotoxicity effect on MCF-7 cell line in concentration range between 5 to 100µg/ml, with IC 50 (µg/ml) value is 26.2. These plants have good antimicrobial activity, further investigation on the phytochemistry of bioactive compounds of these plants would result in discovery of new drugs and further pharmacological investigation of anti-cancer activity of Aeridis odarata should be done.

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