scholarly journals The Effect of Advanced Motherhood on Newborn Offspring’s Hippocampal Neural Stem Cell Proliferation

2016 ◽  
Vol 2016 ◽  
pp. 1-7
Author(s):  
Bo Li ◽  
Ping Duan ◽  
Xuefei Han ◽  
Wenhai Yan ◽  
Ying Xing
Keyword(s):  
Cell Cycle ◽  
Stem Cells ◽  
Cell Proliferation ◽  
Stem Cell ◽  
Neural Stem Cell ◽  
Control Group ◽  
Stem Cell Proliferation ◽  
Neural Stem Cell Proliferation ◽  
Positive Rate ◽  
Mother Group

Objective. To investigate the effect of advanced motherhood on rat hippocampal neural stem cell proliferation.Methods. Female parents were subdivided into control and old mother group by age, and neural stem cells were cultured from hippocampal tissues for 24 h newborn offspring. The diameter and numbers of neurospheres were examined by microscopy, and differences in proliferation were examined by EdU immunofluorescence, CCK-8 assay, and cell cycle analysis.Results. The number of neurospheres in the old mother group after culture was lower than the control group. Additionally, neurospheres’ diameter was smaller than that of the control group (P<0.05). The EdU positive rate of the old mother group was lower than that of the control group (P<0.05). CCK-8 assay results showed that the absorbance values for the old mother group were lower than that of the control group at 48 h and 72 h (P<0.05). The proportions of cells in the S and G2/M phases of the cell cycle for the older mother group were less than that found for the control group (P<0.05).Conclusion. The proliferation rates of hippocampal NSCs seen in the older mother group were lower than that seen in the control group.

scholarly journals MicroRNA let-7b regulates neural stem cell proliferation and differentiation by targeting nuclear receptor TLX signaling

2010 ◽  
Vol 107 (5) ◽  
pp. 1876-1881 ◽  
Author(s):  
Chunnian Zhao ◽  
GuoQiang Sun ◽  
Shengxiu Li ◽  
Ming-Fei Lang ◽  
Su Yang ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Proliferation ◽  
Stem Cell ◽  
Cyclin D1 ◽  
Neural Stem Cell ◽  
Neural Differentiation ◽  
Stem Cell Proliferation ◽  
Neural Stem Cell Proliferation ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation

Neural stem cell self-renewal and differentiation is orchestrated by precise control of gene expression involving nuclear receptor TLX. Let-7b, a member of the let-7 microRNA family, is expressed in mammalian brains and exhibits increased expression during neural differentiation. However, the role of let-7b in neural stem cell proliferation and differentiation remains unknown. Here we show that let-7b regulates neural stem cell proliferation and differentiation by targeting the stem cell regulator TLX and the cell cycle regulator cyclin D1. Overexpression of let-7b led to reduced neural stem cell proliferation and increased neural differentiation, whereas antisense knockdown of let-7b resulted in enhanced proliferation of neural stem cells. Moreover, in utero electroporation of let-7b to embryonic mouse brains led to reduced cell cycle progression in neural stem cells. Introducing an expression vector of Tlx or cyclin D1 that lacks the let-7b recognition site rescued let-7b-induced proliferation deficiency, suggesting that both TLX and cyclin D1 are important targets for let-7b-mediated regulation of neural stem cell proliferation. Let-7b, by targeting TLX and cyclin D1, establishes an efficient strategy to control neural stem cell proliferation and differentiation.

scholarly journals Loss of ZC4H2 and RNF220 Inhibits Neural Stem Cell Proliferation and Promotes Neuronal Differentiation

Cells ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 1600
Author(s):  
Longlong Zhang ◽  
Maosen Ye ◽  
Liang Zhu ◽  
Jingmei Cha ◽  
Chaocui Li ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Proliferation ◽  
Stem Cell ◽  
Neural Stem Cell ◽  
Stem Cell Proliferation ◽  
Neural Stem Cell Proliferation ◽  
Protein Levels ◽  
Ubiquitin E3 Ligase ◽  
Proliferation And Differentiation

The ubiquitin E3 ligase RNF220 and its co-factor ZC4H2 are required for multiple neural developmental processes through different targets, including spinal cord patterning and the development of the cerebellum and the locus coeruleus. Here, we explored the effects of loss of ZC4H2 and RNF220 on the proliferation and differentiation of neural stem cells (NSCs) derived from mouse embryonic cortex. We showed that loss of either ZC4H2 or RNF220 inhibits the proliferation and promotes the differentiation abilities of NSCs in vitro. RNA-Seq profiling revealed 132 and 433 differentially expressed genes in the ZC4H2−/− and RNF220−/− NSCs, compared to wild type (WT) NSCs, respectively. Specifically, Cend1, a key regulator of cell cycle exit and differentiation of neuronal precursors, was found to be upregulated in both ZC4H2−/− and RNF220−/− NSCs at the mRNA and protein levels. The targets of Cend1, such as CyclinD1, Notch1 and Hes1, were downregulated both in ZC4H2−/− and RNF220−/− NSCs, whereas p53 and p21 were elevated. ZC4H2−/− and RNF220−/− NSCs showed G0/G1 phase arrest compared to WT NSCs in cell cycle analysis. These results suggested that ZC4H2 and RNF220 are likely involved in the regulation of neural stem cell proliferation and differentiation through Cend1.

scholarly journals Histone Demethylase LSD1 Regulates Neural Stem Cell Proliferation

2010 ◽  
Vol 30 (8) ◽  
pp. 1997-2005 ◽  
Author(s):  
GuoQiang Sun ◽  
Kamil Alzayady ◽  
Richard Stewart ◽  
Peng Ye ◽  
Su Yang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Proliferation ◽  
Stem Cell ◽  
Neural Stem Cells ◽  
Neural Stem Cell ◽  
Target Genes ◽  
Neural Progenitor ◽  
Stem Cell Proliferation ◽  
Neural Stem Cell Proliferation ◽  
Cell Proliferation Inhibition

ABSTRACT Lysine-specific demethylase 1 (LSD1) functions as a transcriptional coregulator by modulating histone methylation. Its role in neural stem cells has not been studied. We show here for the first time that LSD1 serves as a key regulator of neural stem cell proliferation. Inhibition of LSD1 activity or knockdown of LSD1 expression led to dramatically reduced neural stem cell proliferation. LSD1 is recruited by nuclear receptor TLX, an essential neural stem cell regulator, to the promoters of TLX target genes to repress the expression of these genes, which are known regulators of cell proliferation. The importance of LSD1 function in neural stem cells was further supported by the observation that intracranial viral transduction of the LSD1 small interfering RNA (siRNA) or intraperitoneal injection of the LSD1 inhibitors pargyline and tranylcypromine led to dramatically reduced neural progenitor proliferation in the hippocampal dentate gyri of wild-type adult mouse brains. However, knockout of TLX expression abolished the inhibitory effect of pargyline and tranylcypromine on neural progenitor proliferation, suggesting that TLX is critical for the LSD1 inhibitor effect. These findings revealed a novel role for LSD1 in neural stem cell proliferation and uncovered a mechanism for neural stem cell proliferation through recruitment of LSD1 to modulate TLX activity.

Could the endogenous opioid, morphine, prevent neural stem cell proliferation?

2011 ◽  
Vol 76 (2) ◽  
pp. 225-229 ◽  
Author(s):  
Alireza Shoae-Hassani ◽  
Shiva Sharif ◽  
Seyed Abdolreza Mortazavi Tabatabaei ◽  
Javad Verdi
Keyword(s):  
Cell Proliferation ◽  
Stem Cell ◽  
Neural Stem Cell ◽  
Endogenous Opioid ◽  
Stem Cell Proliferation ◽  
Neural Stem Cell Proliferation

Activation of adenosine A1receptor-induced neural stem cell proliferation via MEK/ERK and Akt signaling pathways

2008 ◽  
Vol 86 (13) ◽  
pp. 2820-2828 ◽  
Author(s):  
Hideyuki Migita ◽  
Katsuya Kominami ◽  
Mami Higashida ◽  
Rumi Maruyama ◽  
Nobuko Tuchida ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Stem Cell ◽  
Signaling Pathways ◽  
Neural Stem Cell ◽  
Akt Signaling ◽  
Stem Cell Proliferation ◽  
Neural Stem Cell Proliferation ◽  
Induced Neural Stem Cell

scholarly journals Rac1 Guides Porf-2 to Wnt Pathway to Mediate Neural Stem Cell Proliferation

2017 ◽  
Vol 10 ◽  
Author(s):  
Xi-Tao Yang ◽  
Guo-Hui Huang ◽  
Hong-Jiang Li ◽  
Zhao-Liang Sun ◽  
Nan-Jie Xu ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Stem Cell ◽  
Neural Stem Cell ◽  
Wnt Pathway ◽  
Stem Cell Proliferation ◽  
Neural Stem Cell Proliferation
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