scholarly journals Antiaging Effect ofInula britannicaon Aging Mouse Model Induced by D-Galactose

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Hui Chen ◽  
Yuanyuan Long ◽  
Lei Guo
Keyword(s):  
Histological Analysis ◽  
Skin Aging ◽  
Point Of View ◽  
Skin Tissue ◽  
Antioxidant Ability ◽  
Mrna Expression Level ◽  
Dermal Thickness ◽  
Skin Collagen ◽  
Inula Britannica ◽  
Mda Content

The antiaging effect ofInula britannicaflower total flavonoids (IBFTF) on aging mice induced by D-galactose and its mechanism was examined in this study. From the results, the biochemical indexes and histological analysis of skin tissues showed that IBFTF could effectively improve the antioxidant enzyme activity of the aging mice, enhance the activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) of skin tissue, and decrease the malondialdehyde (MDA) content. Besides, IBFTF could maintain the skin collagen, hydroxyproline (Hyp), dermal thickness, and moisture content. Meanwhile, IBFTF could significantly reduce the number of cells arrested in G0/G1 phase, and from the point of view of protein and mRNA expression level in skin tissue, IBFTF could significantly increase the expression of Sirt1 and CyclinD1 but decrease the expression of p16 and p21, and its effect was not less than that of the well-known vitamin E (VE). Overall, these results seem to be implying that IBFTF is a potential natural anti-skin aging agent with great antioxidant ability.

scholarly journals Polysaccharide Extracted fromLaminaria japonicaDelays Intrinsic Skin Aging in Mice

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Longyuan Hu ◽  
Jia Tan ◽  
Xiaomei Yang ◽  
Haitao Tan ◽  
Xiaozhen Xu ◽  
...  
Keyword(s):  
Skin Aging ◽  
Skin Tissue ◽  
Dorsal Skin ◽  
Young Control ◽  
Dermal Thickness ◽  
Skin Collagen ◽  
Electron Microscopy Analysis ◽  
Microscopy Analysis ◽  
Skin Samples ◽  
Aging Skin

This study aimed to determine the effect of topically appliedLaminariapolysaccharide (LP) on skin aging. We applied ointment containing LP (10, 25, and 50 μg/g) or vitamin E (10 μg/g) to the dorsal skin of aging mice for 12 months and young control mice for 4 weeks. Electron microscopy analysis of skin samples revealed that LP increased dermal thickness and skin collagen content. Tissue inhibitor of metalloprotease- (TIMP-) 1 expression was upregulated while that of matrix metalloproteinase- (MMP-) 1 was downregulated in skin tissue of LP-treated as compared to untreated aging mice. Additionally, phosphorylation of c-Jun N-terminal kinase (JNK) and p38 was higher in aging skin than in young skin, while LP treatment suppressed phospho-JNK expression. LP application also enhanced the expression of antioxidative enzymes in skin tissue, causing a decrease in malondialdehyde levels and increases in superoxide dismutase, catalase, and glutathione peroxidase levels relative to those in untreated aging mice. These results indicate that LP inhibits MMP-1 expression by preventing oxidative stress and JNK phosphorylation, thereby delaying skin collagen breakdown during aging.

scholarly journals In VivoAntioxidant and Anti-Skin-Aging Activities of Ethyl Acetate Extraction fromIdesia polycarpaDefatted Fruit Residue in Aging Mice Induced by D-Galactose

2014 ◽  
Vol 2014 ◽  
pp. 1-12 ◽  
Author(s):  
Yang Ye ◽  
Ran-ran Jia ◽  
Lin Tang ◽  
Fang Chen
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Radical Scavenging ◽  
Antioxidant Defense System ◽  
Skin Aging ◽  
Age Related ◽  
Skin Collagen ◽  
Total Antioxidant ◽  
Mda Content

Two different concentrations of D-galactose (D-gal) induced organism and skin aging in Kunming mice were used to examine comprehensively the antioxidant and antiaging activities of ethyl acetate extraction (EAE) fromIdesia polycarpadefatted fruit residue for the first time. The oxygen radical absorbance capacity (ORAC) of EAE was 13.09 ± 0.11 μmol Trolox equivalents (TE)/mg, which showed EAE had greatin vitrofree radical scavenging and antioxidant activity. Biochemical indexes and morphological analysis of all tested tissues showed that EAE could effectively improve the total antioxidant capacity (T-AOC) of the antioxidant defense system of the aging mice, enhance the activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) of tissues and serum, increase glutathione (GSH) content and decrease the malondialdehyde (MDA) content, and maintain the skin collagen, elastin, and moisture content. Meanwhile, EAE could effectively attenuate the morphological damage in brain, liver, kidney, and skin induced by D-gal and its effect was not less than that of the well-known L-ascorbic acid (VC) andα-tocopherol (VE). Overall, EAE is a potent natural antiaging agent with great antioxidant activity, which can be developed as a new medicine and cosmetic for the treatment of age-related conditions.

scholarly journals Effect Of Water Clover (Marsilea Crenata) Leaf Extract On Estrogen Receptors-Β In Skin Aging

2021 ◽  
Vol 14 (1) ◽  
pp. 68-76
Author(s):  
Winawati eka putri Wina ◽  
Maria ulfa Maria
Keyword(s):  
Leaf Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Skin Aging ◽  
Negative Control ◽  
Control Group ◽  
Human Consumption ◽  
Hormonal Changes ◽  
Dermal Thickness ◽  
Skin Collagen

Extrinsic and intrinsic factors influence skin aging. Hormonal changes, especially estrogen, significantly affect intrinsic skin aging. Decreased circulating estrogen levels reduce skin collagen content and skin elasticity. Isoflavones in Marsilea crenata (MC) leaf are active substances containing compounds that mimic estrogen. This study aims to analyze MC leaf extract against the estrogen receptor (ER)-β. The sample for this research was female Wistar Rats (Rattus norwegicus). All of them were 12 months old, with their weight was between 350 to 550 grams divided into five groups. P1 P2 and P3 were grouping with MC leaf extract administration in sequential doses 20 mg/kg BW, 30 mg/kg BW, and 40 mg/kg BW. At the same time, P4 was a positive control group, and P5 was a negative control group. The independent variable was M.crenata leaf extract. The dependent variables were (ER)-β expression and dermal thickness. The data analysis utilized the one-way analysis of variance (ANOVA) showed significant differences (p< 0.05). Each group showed significant results, and group P2 showed the highest expression of (ER)-β and dermal thickness. The result showed that there were significant correlations between both variables (P<0.05). This research has proved that water clover extract could become an alternative treatment in the future for skin aging. However, further research should find a proper dose for human consumption.

scholarly journals Antioxidant, Anti-Inflammatory, and Anti-Aging Potential of a Kalmia angustifolia Extract and Identification of Some Major Compounds

Antioxidants ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 1373 ◽  
Author(s):  
Alexe Grenier ◽  
Jean Legault ◽  
André Pichette ◽  
Lorry Jean ◽  
Audrey Bélanger ◽  
...  
Keyword(s):  
Biological Activities ◽  
Skin Aging ◽  
Inflammatory Activity ◽  
No Production ◽  
Skin Substitutes ◽  
Kalmia Angustifolia ◽  
Isolation And Characterization ◽  
Assembly Method ◽  
Dermal Thickness ◽  
Anti Inflammatory

Skin aging is the most visible element of the aging process, giving rise to a major concern for many people. Plants from the Ericaceae family generally have antioxidant and anti-inflammatory properties, making them potential anti-aging active ingredients. This study aimed to evaluate the safety and anti-aging efficacy of a Kalmia angustifolia extract using reconstructed skin substitutes. The safety evaluation was performed using a 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) assay, while the efficacy was determined by assessing antioxidant and anti-inflammatory activity and analyzing skin substitutes reconstructed according to the self-assembly method by histology and immunofluorescence staining (elastin, collagen-1, collagen-3, aquaporin-3). The cell viability assay established the safety of the extract at a concentration up to 200 μg/mL. The Oxygen Radical Absorbance Capacity (ORAC) assay and a cell-based assay using 2’,7’-dichlorofluorescein-diacetate (DCFH-DA) revealed a strong antioxidant activity with an ORAC value of 16 µmol Trolox Equivalent/mg and a half-maximal inhibitory concentration (IC50) of 0.37 ± 0.02 μg/mL, while an interesting anti-inflammatory activity was found in the inhibition of NO production, with an inhibition percentage of NO production of 49 ± 2% at 80 µg/mL. The isolation and characterization of the extract allowed the identification of compounds that could be responsible for these biological activities, with two of them being identified for the first time in K. angustifolia: avicularin and epicatechin-(2β-O-7, 4β-6)-ent-epicatechin. Histological analyses of skin substitutes treated with the extract showed an increase in dermal thickness compared with the controls. K. angustifolia extract enhanced the expression of elastin and collagen-1, which are usually decreased with skin aging. These results suggest that K. angustifolia has promising antioxidant efficacy and anti-aging potential.

scholarly journals An Oenothera biennis Cell Cultures Extract Endowed with Skin Anti-Ageing Activity Improves Cell Mechanical Properties

Metabolites ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 527
Author(s):  
Sara Ceccacci ◽  
Adriana De Lucia ◽  
Annalisa Tito ◽  
Assunta Tortora ◽  
Danila Falanga ◽  
...  
Keyword(s):  
Tissue Repair ◽  
Actin Polymerization ◽  
Ex Vivo ◽  
Skin Aging ◽  
Point Of View ◽  
Elastic Fibers ◽  
Oenothera Biennis ◽  
Contraction Process ◽  
Collagen Contraction

Skin aging is a very well-known process setting a gradual worsening of skin mechanical features due to a decline in the production of the extra-cellular matrix machinery and to a concurrent change in the contraction process. To slow this progression, it is crucial to induce the expression of several proteins able to promote elastic fibers formation and tissue repair. Here, the Oenothera biennis cell culture aqueous extract has been investigated from a chemical point of view and then it was tested in vitro, in cell, and in ex vivo experiments as adjuvant in counteracting skin aging. Accordingly, it has been shown that the Oenothera biennis extract was able, by increasing MYLK gene expression, to promote matrix collagen contraction, actin polymerization, and the production of essential ECM proteins.

Effect of L-fucose and fucose-rich oligo- and polysaccharides (FROP-s) on skin aging: penetration, skin tissue production and fibrillogenesis

2003 ◽  
Vol 57 (5-6) ◽  
pp. 209-215 ◽  
Author(s):  
I Fodil-Bourahla ◽  
L Bizbiz ◽  
D Schoevaert ◽  
A.M Robert ◽  
L Robert
Keyword(s):  
Skin Aging ◽  
Skin Tissue ◽  
Tissue Production

Changes in the Composition of the Cornified Envelope During Skin Aging: A Calcium Centric Point of View

2016 ◽  
pp. 265-284
Author(s):  
Maria Karolin Streubel ◽  
Mark Rinnerthaler ◽  
Johannes Bischof ◽  
Klaus Richter
Keyword(s):  
Skin Aging ◽  
Point Of View ◽  
Cornified Envelope

Adipose Collagen Fragment: A Novel Adipose-Derived Extracellular Matrix Concentrate for Skin Filling

2021 ◽  
Author(s):  
Yuchen Zhang ◽  
Xiangdong Zhang ◽  
Xiaoxuan Jin ◽  
Pan Zhang ◽  
Kaiyang Liu ◽  
...  
Keyword(s):  
Skin Aging ◽  
Therapeutic Effects ◽  
Dorsal Skin ◽  
Skin Rejuvenation ◽  
Collagen Remodeling ◽  
Patients Satisfaction ◽  
Dermal Thickness ◽  
Collagen Fragment ◽  
Phosphate Buffered Saline ◽  
Filling Capacity

Abstract Background Skin filler is an option for treatment of skin aging and wrinkle formation; however, currently used fillers are limited by poor biocompatibility, rapid degradation, and possible hypersensitivity reactions. However, autologous adipose tissue-derived products have been recognized as promising options for skin rejuvenation. Objectives This study aimed to develop a novel adipose-derived product for skin filling. Methods Adipose collagen fragment (ACF) was prepared through pulverization, filtration, and centrifugation. The macrography, structure, types of collagen, and cell viability of ACF were evaluated by immunostaining, Western blotting, and cell culture assays. ACF, nanofat and phosphate-buffered saline (9 spots/side, 0.01 ml/spot) were intradermally injected in the dorsal skin of 36 female BALB/c nude mice; then, the skin filling capacity and collagen remodeling process were investigated. Twenty-one female patients with fine rhytides in the infraorbital areas were enrolled and received ACF treatment as clinical applications. Therapeutic effects and patients’ satisfaction scores were recorded. Results The ACF yield from 50 ml of Coleman fat was 4.91 ± 0.25 ml. ACF contained nonviable cells and high levels of collagen I, collagen IV, and laminin. Fibroblasts and procollagen significantly increased in ACF and ACF-treated dermis (p &lt; 0.05). 85.7% of patients were satisfied with the therapy results, and no infections, injection site nodules, or other unwanted side effects were observed. Conclusions ACF significantly improved dermal thickness and collagen synthesis and may serve as a potential autologous skin filler.

scholarly journals Skin Aging & Modern Age Anti-aging Strategies

2019 ◽  
pp. 15-60
Author(s):  
Abdul Kader Mohiuddin
Keyword(s):  
Structural Changes ◽  
Skin Aging ◽  
Layered Composite ◽  
Point Of View ◽  
The Body ◽  
Hormonal Changes ◽  
Adjacent Layer ◽  
Functional Changes ◽  
Extracellular Matrix Components ◽  
Physiological And Biochemical

As the most voluminous organ of the body that is exposed to the outer environment, the skin suffers from both intrinsic and extrinsic aging factors. Skin aging is characterized by features such as wrinkling, loss of elasticity, laxity, and rough-textured appearance. This aging process is accompanied with phenotypic changes in cutaneous cells as well as structural and functional changes in extracellular matrix components such as collagens and elastin. With intrinsic aging, structural changes occur in the skin as a natural consequence of the biological changes over time and produce a certain number of histological, physiological, and biochemical modifications. Intrinsic aging is determined genetically (influence of gender and ethnic group), variable in function of skin site, and also influenced by hormonal changes. Visually it is characterized by fine wrinkles. By comparison, “photoaging” is the term used to describe the changes occurring in the skin, resulting from repetitive exposure to sunlight. The histological, physiological, and biochemical changes in the different layers of the skin are much more drastic. From a mechanical point of view, human skin appears as a layered composite containing the stiff thin cover layer presented by the stratum corneum, below which are the more compliant layers of viable epidermis and dermis and further below the much more compliant adjacent layer of subcutaneous white adipose tissue.

Changes in the Composition of the Cornified Envelope During Skin Aging: A Calcium Centric Point of View

2015 ◽  
pp. 1-20
Author(s):  
Maria Karolin Streubel ◽  
Mark Rinnerthaler ◽  
Johannes Bischof ◽  
Klaus Richter
Keyword(s):  
Skin Aging ◽  
Point Of View ◽  
Cornified Envelope
Sign in / Sign up

Export Citation Format

Share Document