scholarly journals Corrigendum to “Adipose-Derived Mesenchymal Stem Cells in the Regeneration of Vocal Folds: A Study on a Chronic Vocal Fold Scar”

2016 ◽  
Vol 2016 ◽  
pp. 1-1 ◽  
Author(s):  
Valerie Angelou ◽  
Vassiliki Kalodimou ◽  
Irini Messini ◽  
Nikolaos Psychalakis ◽  
Eleftheria Karampela ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Vocal Fold ◽  
Vocal Folds ◽  
Vocal Fold Scar

The Ability of Human Nasal Inferior Turbinate–Derived Mesenchymal Stem Cells to Repair Vocal Fold Injuries

2018 ◽  
Vol 159 (2) ◽  
pp. 335-342 ◽  
Author(s):  
Choung-Soo Kim ◽  
Hyunsu Choi ◽  
Ki Cheol Park ◽  
Sung Won Kim ◽  
Dong-Il Sun
Keyword(s):  
Stem Cells ◽  
Polymerase Chain Reaction ◽  
Wound Healing ◽  
Mesenchymal Stem Cells ◽  
Vocal Fold ◽  
Inferior Turbinate ◽  
Vocal Folds ◽  
Histopathological Analysis ◽  
Chain Reaction ◽  
Polymerase Chain

Objective This study investigated the ability of implanted human nasal inferior turbinate–derived mesenchymal stem cells (hTMSCs) to repair injured vocal folds. To this end, we used quantitative real-time polymerase chain reaction (PCR) to analyze the early phase of wound healing and histopathological analysis to explore the late phase of wound healing in xenograft animal models. Study Design Prospective animal study. Setting Research laboratory. Subjects and Methods The right-side lamina propria of the vocal fold was injured in 20 rabbits and 30 rats. Next, hTMSCs were implanted into half of the injured vocal folds (hTMSC groups). As a control, phosphate-buffered saline (PBS) was injected into the other half of the injured vocal folds (PBS groups). Rat vocal folds were harvested for polymerase chain reaction (PCR) at 1 week after injury. Rabbit vocal folds were evaluated endoscopically and the larynges harvested for histological and immunohistochemical examination at 2 and 8 weeks after injury. Results In the hTMSC group, PCR showed that hyaluronan synthase ( HAS) 1, HAS 2, and transforming growth factor ( TGF)–β1 were significantly upregulated compared with the PBS group. Procollagen type III ( COL III) messenger RNA expression was significantly upregulated in the PBS group compared with the normal group. Histological analyses showed that hTMSC administration afforded more favorable collagen and hyaluronic acid deposition than was evident in the controls. Implanted hTMSCs were observed in injured vocal folds 2 weeks after implantation. Conclusions Our results show that hTMSCs implantation into injured vocal folds facilitated vocal fold regeneration, with presenting antifibrotic effects.

scholarly journals Adipose-Derived Mesenchymal Stem Cells in the Regeneration of Vocal Folds: A Study on a Chronic Vocal Fold Scar

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Angelou Valerie ◽  
Kalodimou Vassiliki ◽  
Messini Irini ◽  
Psychalakis Nikolaos ◽  
Eleftheria Karampela ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hyaluronic Acid ◽  
Lamina Propria ◽  
Vocal Fold ◽  
Rabbit Model ◽  
Vocal Folds ◽  
Scar Tissue ◽  
Elastic Fibers ◽  
Adipose Derived Stem Cells ◽  
Vocal Fold Scar

Background. The aim of the study was to assess the histological effects of autologous infusion of adipose-derived stem cells (ADSC) on a chronic vocal fold scar in a rabbit model as compared to an untreated scar as well as in injection of hyaluronic acid.Study Design. Animal experiment.Method. We used 74 New Zealand rabbits. Sixteen of them were used as control/normal group. We created a bilateral vocal fold wound in the remaining 58 rabbits. After 18 months we separated our population into three groups. The first group served as control/scarred group. The second one was injected with hyaluronic acid in the vocal folds, and the third received an autologous adipose-derived stem cell infusion in the scarred vocal folds (ADSC group). We measured the variation of thickness of the lamina propria of the vocal folds and analyzed histopathologic changes in each group after three months.Results. The thickness of the lamina propria was significantly reduced in the group that received the ADSC injection, as compared to the normal/scarred group. The collagen deposition, the hyaluronic acid, the elastin levels, and the organization of elastic fibers tend to return to normal after the injection of ADSC.Conclusions. Autologous injection of adipose-derived stem cells on a vocal fold chronic scar enhanced the healing of the vocal folds and the reduction of the scar tissue, even when compared to other treatments.

scholarly journals Antioxidant Properties of Tonsil-Derived Mesenchymal Stem Cells on Human Vocal Fold Fibroblast Exposed to Oxidative Stress

2020 ◽  
Vol 2020 ◽  
pp. 1-12
Author(s):  
Sung-Chan Shin ◽  
Hyung-Sik Kim ◽  
Yoojin Seo ◽  
Cho Hee Kim ◽  
Ji Min Kim ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cell Viability ◽  
Vocal Fold ◽  
Vocal Folds ◽  
Dependent Manner ◽  
The Impact

The therapeutic potential of tonsil-derived mesenchymal stem cells (TMSCs) has been proved in several in vitro and in vivo models based on their antioxidative capacity. Oxidative stress is involved in the formation of vocal fold scars and the aging of vocal folds. However, few studies have examined the direct correlation between oxidative damage and reconstitution of extracellular matrix (ECM) in the vocal fold fibrosis. We, therefore, sought to investigate the impact of oxidative stress on cell survival and ECM production of human vocal fibroblasts (hVFFs) and the protective effects elicited by TMSCs against oxidative damages in hVFFs. hVFFs were exposed to different concentrations of tert-butyl hydroperoxide in the presence or absence of TMSCs. Cell viability and reactive oxygen species (ROS) production were assessed to examine the progression of oxidative stress in vitro. In addition, expression patterns of ECM-associated factors including various collagens were examined by real-time PCR and immunocytochemical analysis. We found that both cell viability and proliferation capacity of hVFFs were decreased following the exposure to tBHP in a dose-dependent manner. Furthermore, tBHP treatment induced the generation of ROS and reactive aldehydes, while it decreased endogenous activity of antioxidant enzymes in hVFF. Importantly, TMSCs could rescue these oxidative stress-associated damages of hVFFs. TMSCs also downregulated tBHP-mediated production of proinflammatory cytokines in hVFFs. In addition, coculture with TMSC could restore the endogenous matrix metalloproteinase (MMP) activity of hVFFs upon tBHP treatment and, in turn, reduce the oxidative stress-induced ECM accumulation in hVFFs. We have, therefore, shown that the changes in hVFF proliferative capacity and ECM gene expression induced by oxidative stress are consistent with in vivo phenotypes observed in aging vocal folds and vocal fold scarring and that TMSCs may function to reduce oxidative stress in aging vocal folds.

Injection of human mesenchymal stem cells improves healing of vocal folds after scar excision-A xenograft analysis

2011 ◽  
Vol 121 (10) ◽  
pp. 2185-2190 ◽  
Author(s):  
Bengt Svensson ◽  
Srinivasa R. Nagubothu ◽  
Jessica Cedervall ◽  
Roger W. Chan ◽  
Katrina Le Blanc ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells ◽  
Vocal Folds

Regeneration of the Vocal Fold Using Autologous Mesenchymal Stem Cells

2003 ◽  
Vol 112 (11) ◽  
pp. 915-920 ◽  
Author(s):  
Shin-Ichi Kanemaru ◽  
Hisayoshi Kojima ◽  
Shigeru Hirano ◽  
Tatsuo Nakamura ◽  
Akhmar Magrufov ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Vocal Fold ◽  
Autologous Mesenchymal Stem Cells

REGENERATION OF THE VOCAL FOLD USING AUTOLOGOUS MESENCHYMAL STEM CELLS

ASAIO Journal ◽  
2002 ◽  
Vol 48 (2) ◽  
pp. 191
Author(s):  
Shin-ichi Kanemaru ◽  
Tatsuo Nakamura ◽  
Akhmar Magrufov ◽  
Shigeru Hirano ◽  
Juichi Ito ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Vocal Fold ◽  
Autologous Mesenchymal Stem Cells

Injection of human mesenchymal stem cells improves healing of scarred vocal folds: Analysis using a xenograft model

2010 ◽  
Vol 120 (7) ◽  
pp. 1370-1375 ◽  
Author(s):  
Bengt Svensson ◽  
R. Srinivasa Nagubothu ◽  
Jessica Cedervall ◽  
Katarina Le Blanc ◽  
Lars Ährlund-Richter ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Xenograft Model ◽  
Human Mesenchymal Stem Cells ◽  
Vocal Folds

scholarly journals Do mesenchymal stem cells play a role in vocal fold fat graft survival?

2008 ◽  
Vol 41 (3) ◽  
pp. 460-473 ◽  
Author(s):  
V. Lo Cicero ◽  
E. Montelatici ◽  
G. Cantarella ◽  
R. Mazzola ◽  
G. Sambataro ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Graft Survival ◽  
Vocal Fold ◽  
Fat Graft
Sign in / Sign up

Export Citation Format

Share Document