scholarly journals Transcriptome-Based Analysis of Dof Family Transcription Factors and Their Responses to Abiotic Stress in Tea Plant (Camellia sinensis)

2016 ◽  
Vol 2016 ◽  
pp. 1-15 ◽  
Author(s):  
Hui Li ◽  
Wei Huang ◽  
Zhi-Wei Liu ◽  
Yong-Xin Wang ◽  
Jing Zhuang
Keyword(s):  
Transcription Factors ◽  
Abiotic Stress ◽  
Camellia Sinensis ◽  
Stress Resistance ◽  
Abiotic Stress Tolerance ◽  
Plant Stress ◽  
Tea Plant ◽  
Interaction Networks ◽  
Qrt Pcr ◽  
Dof Transcription Factors

Tea plant (Camellia sinensis (L.) O. Kuntze) is affected by abiotic stress during its growth and development. DNA-binding with one finger (Dof) transcription factors (TFs) play important roles in abiotic stress tolerance of plants. In this study, a total of 29 putative Dof TFs were identified based on transcriptome of tea plant, and the conserved domains and common motifs of these CsDof TFs were predicted and analyzed. The 29 CsDof proteins were divided into 7 groups (A, B1, B2, C1, C2.1, C2.2, and D2), and the interaction networks of Dof proteins in C. sinensis were established according to the data in Arabidopsis. Gene expression was analyzed in “Yingshuang” and “Huangjinya” under four experimental stresses by qRT-PCR. CsDof genes were expressed differentially and related to different abiotic stress conditions. In total, our results might suggest that there is a potential relationship between CsDof factors and tea plant stress resistance.

scholarly journals Genomic and transcriptomic analyses of HD-Zip family transcription factors and their responses to abiotic stress in tea plant (Camellia sinensis)

Genomics ◽  
2019 ◽  
Vol 111 (5) ◽  
pp. 1142-1151 ◽  
Author(s):  
Wei Shen ◽  
Hui Li ◽  
Ruimin Teng ◽  
Yongxin Wang ◽  
Wenli Wang ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Abiotic Stress ◽  
Camellia Sinensis ◽  
Tea Plant ◽  
Zip Family

scholarly journals Molecular Identification and Characterization of Hydroxycinnamoyl Transferase in Tea Plants (Camellia sinensis L.)

2018 ◽  
Vol 19 (12) ◽  
pp. 3938 ◽  
Author(s):  
Chi-Hui Sun ◽  
Chin-Ying Yang ◽  
Jason Tzen
Keyword(s):  
Abiotic Stress ◽  
Amino Acid ◽  
Camellia Sinensis ◽  
Polymerase Chain Reaction Analysis ◽  
Coffea Canephora ◽  
Tea Plant ◽  
Amino Acid Sequences ◽  
Hibiscus Cannabinus ◽  
Expression Level ◽  
Tea Plants

Tea (Camellia sinensis L.) contains abundant secondary metabolites, which are regulated by numerous enzymes. Hydroxycinnamoyl transferase (HCT) is involved in the biosynthesis pathways of polyphenols and flavonoids, and it can catalyze the transfer of hydroxyconnamoyl coenzyme A to substrates such as quinate, flavanol glycoside, or anthocyanins, thus resulting in the production of chlorogenic acid or acylated flavonol glycoside. In this study, the CsHCT gene was cloned from the Chin-Shin Oolong tea plant, and its protein functions and characteristics were analyzed. The full-length cDNA of CsHCT contains 1311 base pairs and encodes 436 amino acid sequences. Amino acid sequence was highly conserved with other HCTs from Arabidopsis thaliana, Populus trichocarpa, Hibiscus cannabinus, and Coffea canephora. Quantitative real-time polymerase chain reaction analysis showed that CsHCT is highly expressed in the stem tissues of both tea plants and seedlings. The CsHCT expression level was relatively high at high altitudes. The abiotic stress experiment suggested that low temperature, drought, and high salinity induced CsHCT transcription. Furthermore, the results of hormone treatments indicated that abscisic acid (ABA) induced a considerable increase in the CsHCT expression level. This may be attributed to CsHCT involvement in abiotic stress and ABA signaling pathways.

scholarly journals Low Caffeine Content in Novel Grafted Tea with Camellia sinensis as Scions and Camellia oleifera as Stocks

2015 ◽  
Vol 10 (5) ◽  
pp. 1934578X1501000 ◽  
Author(s):  
Wei-Wei Deng ◽  
Min Li ◽  
Chen-Chen Gu ◽  
Da-Xiang Li ◽  
Lin-Long Ma ◽  
...  
Keyword(s):  
Camellia Sinensis ◽  
Expression Patterns ◽  
Tea Plant ◽  
Protein Accumulation ◽  
Tea Leaves ◽  
Qrt Pcr ◽  
Caffeine Content ◽  
Caffeine Synthase ◽  
Specificity And Sensitivity ◽  
Tea Plants

Caffeine, a purine alkaloid, is a major secondary metabolite in tea leaves. The demand for low caffeine tea is increasing in recent years, especially for health reasons. We report a novel grafted tea material with low caffeine content. The grafted tea plant had Camellia sinensis as scions and C. oleifera as stocks. The content of purine alkaloids was determined in the leaves of one-year-old grafted tea plants by HPLC. We also characterized caffeine synthase (CS), a key enzyme involved in caffeine biosynthesis in tea plants, at the expression level. The expression patterns of CS were examined in grafted and control leaves by Western blot, using a self-prepared polyclonal antibody with high specificity and sensitivity. The expression of related genes ( TCS1, tea caffeine synthase gene, GenBank accession No. AB031280; sAMS, SAM synthetase gene, AJ277206; TIDH, IMP dehydrogenase gene, EU106658) in the caffeine biosynthetic pathway was investigated by qRT-PCR. HPLC showed that the caffeine content was only 38% as compared with the non-grafted tea leaves. Immunoblotting analysis showed that CS protein decreased by half in the leaves of grafted tea plants. qRT-PCR revealed no significant changes in the expression of two genes in the upstream pathway ( sAMS and TIDH), while the expression of TCS1 was greatly decreased (50%). Taken together, these data revealed that the low caffeine content in the grafted tea leaves is due to low TCS1 expression and CS protein accumulation.

Identification, expression, and putative target gene analysis of nuclear factor-Y (NF-Y) transcription factors in tea plant (Camellia sinensis)

Planta ◽  
2019 ◽  
Vol 250 (5) ◽  
pp. 1671-1686 ◽  
Author(s):  
Pengjie Wang ◽  
Yucheng Zheng ◽  
Yongchun Guo ◽  
Xuejin Chen ◽  
Yun Sun ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Camellia Sinensis ◽  
Target Gene ◽  
Tea Plant ◽  
Gene Analysis ◽  
Nuclear Factor ◽  
Putative Target Gene ◽  
Nuclear Factor Y ◽  
Putative Target
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