scholarly journals Enhanced Stem Cell Osteogenic Differentiation by Bioactive Glass Functionalized Graphene Oxide Substrates

2016 ◽  
Vol 2016 ◽  
pp. 1-11 ◽  
Author(s):  
Xiaoju Mo ◽  
Yan Wei ◽  
Xuehui Zhang ◽  
Qing Cai ◽  
Yang Shen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Graphene Oxide ◽  
Stem Cell ◽  
Bioactive Glass ◽  
Osteogenic Differentiation ◽  
Sol Gel ◽  
Functionalized Graphene ◽  
Functionalized Graphene Oxide ◽  
Different Content ◽  
Hybrid Scaffolds

An unmet need in engineered bone regeneration is to develop scaffolds capable of manipulating stem cells osteogenesis. Graphene oxide (GO) has been widely used as a biomaterial for various biomedical applications. However, it remains challenging to functionalize GO as ideal platform for specifically directing stem cell osteogenesis. Herein, we report facile functionalization of GO with dopamine and subsequent bioactive glass (BG) to enhance stem cell adhesion, spreading, and osteogenic differentiation. On the basis of graphene, we obtained dopamine functionalized graphene oxide/bioactive glass (DGO/BG) hybrid scaffolds containing different content of DGO by loading BG nanoparticles on graphene oxide surface using sol-gel method. To enhance the dispersion stability and facilitate subsequent nucleation of BG in GO, firstly, dopamine (DA) was used to modify GO. Then, the modified GO was functionalized with bioactive glass (BG) using sol-gel method. The adhesion, spreading, and osteoinductive effects of DGO/BG scaffold on rat bone marrow mesenchymal stem cells (rBMSCs) were evaluated. DGO/BG hybrid scaffolds with different content of DGO could influence rBMSCs’ behavior. The highest expression level of osteogenic markers suggests that the DGO/BG hybrid scaffolds have great potential or elicit desired bone reparative outcome.

scholarly journals Stimulation of bone formation by monocyte-activator functionalized graphene oxide in vivo

Nanoscale ◽  
2019 ◽  
Vol 11 (41) ◽  
pp. 19408-19421 ◽  
Author(s):  
Valentina Bordoni ◽  
Giacomo Reina ◽  
Marco Orecchioni ◽  
Giulia Furesi ◽  
Stefanie Thiele ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Graphene Oxide ◽  
Calcium Phosphate ◽  
Bone Formation ◽  
Functionalized Graphene ◽  
Functionalized Graphene Oxide ◽  
Calcium Phosphate Nanoparticles ◽  
Stimulation Of

Graphene oxide complexed with calcium phosphate nanoparticles enhances bone regeneration through signalling between monocytes and mesenchymal stem cells.

scholarly journals Fabrication and Characterization of a Biomaterial Based on Extracellular-Vesicle Functionalized Graphene Oxide

2021 ◽  
Vol 9 ◽  
Author(s):  
Julia Driscoll ◽  
Anuradha Moirangthem ◽  
Irene K. Yan ◽  
Tushar Patel
Keyword(s):  
Graphene Oxide ◽  
Stem Cell ◽  
Biological Effects ◽  
Organ Injury ◽  
Hepatocyte Proliferation ◽  
Therapeutic Effects ◽  
Solid Organ ◽  
Functionalized Graphene ◽  
Functionalized Graphene Oxide

Mesenchymal stem cell (MSC) derived extracellular vesicles (EV) are emerging as acellular therapeutics for solid organ injury and as carriers for drug delivery. Graphene-based materials are novel two-dimensional crystal structure-based materials with unique characteristics of stiffness, strength and elasticity that are being explored for various structural and biological applications. We fabricated a biomaterial that would capture desirable properties of both graphene and stem cell derived EV. Metabolically engineered EV that express azide groups were cross-linked with alkyne-functionalized graphene oxide (GO) via a copper catalyzed alkyne-azide cycloaddition (CuAAC) reaction. The crosslinking between EV and GO was accomplished without the need for ligand expression on the metal. Scanning electron and fluorescence microscopy demonstrated excellent cross-linking between EV and GO. Biological effects were assessed by phagocytosis studies and cell viability studies. The uptake of GO or sonicated GO (sGO) resulted in a durable pro-inflammatory immune response. Cell studies further showed that crosslinked GO-EV scaffolds exhibited cell-type dependent cytotoxicity on liver cancer cells whereas there was minimal impact on healthy hepatocyte proliferation. In vitro, neither GO-EV nor sGO-EV induced DNA strand breaks. In vivo studies in zebrafish revealed gross developmental malformations but treatment-induced mortality was only seen with the highest doses of GO-EV and sGO-EV. With these advantages, this engineered biomaterial combining the versatility of graphene with the therapeutic effects of MSC-EV has potential for applications in tissue engineering and regenerative medicine.

scholarly journals Dataset on cytotoxicity effect of polyethylenimine-functionalized graphene oxide nanoparticles on the human embryonic carcinoma stem cell, NTERA2 cell line

Data in Brief ◽  
2019 ◽  
Vol 26 ◽  
pp. 104487
Author(s):  
Zahra Sadeghi ◽  
Parichehr Maleki ◽  
Seyed Abolghasem Mohammadi Bondarkhilli ◽  
Mehdi Mohammadi ◽  
Jamshid Raheb
Keyword(s):  
Graphene Oxide ◽  
Stem Cell ◽  
Cell Line ◽  
Oxide Nanoparticles ◽  
Functionalized Graphene ◽  
Functionalized Graphene Oxide ◽  
Embryonic Carcinoma

scholarly journals Effects on Growth and Osteogenic Differentiation of Mesenchymal Stem Cells by the Zinc-Added Sol-Gel Bioactive Glass Granules

2010 ◽  
Vol 1 (1) ◽  
pp. 475260 ◽  
Author(s):  
Sun-Ae Oh ◽  
So-Hee Kim ◽  
Jong-Eun Won ◽  
Jung-Ju Kim ◽  
Ueon Sang Shin ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bioactive Glass ◽  
Osteogenic Differentiation ◽  
Sol Gel
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