scholarly journals Assessment for Melting Temperature Measurement of Nucleic Acid by HRM

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Jing Wang ◽  
Xiaoming Pan ◽  
Xingguo Liang
Keyword(s):  
Thermal Stability ◽  
Nucleic Acid ◽  
Melting Temperature ◽  
High Sensitivity ◽  
Structural Elements ◽  
Sequence Dependence ◽  
Mutation Scanning ◽  
Dna Triplex ◽  
Uv Melting ◽  
Thermal Stability Of

High resolution melting (HRM), with a high sensitivity to distinguish the nucleic acid species with small variations, has been widely applied in the mutation scanning, methylation analysis, and genotyping. For the aim of extending HRM for the evaluation of thermal stability of nucleic acid secondary structures on sequence dependence, we investigated effects of the dye of EvaGreen, metal ions, and impurities (such as dNTPs) on melting temperature (Tm) measurement by HRM. The accuracy of HRM was assessed as compared with UV melting method, and little difference between the two methods was found when the DNA Tm was higher than 40°C. Both insufficiency and excessiveness of EvaGreen were found to give rise to a little bit higher Tm, showing that the proportion of dye should be considered for precise Tm measurement of nucleic acids. Finally, HRM method was also successfully used to measure Tms of DNA triplex, hairpin, and RNA duplex. In conclusion, HRM can be applied in the evaluation of thermal stability of nucleic acid (DNA or RNA) or secondary structural elements (even when dNTPs are present).

scholarly journals Thermal Stability of Peptide Nucleic Acid Complexes

2019 ◽  
Vol 123 (39) ◽  
pp. 8168-8177 ◽  
Author(s):  
Maciej Jasiński ◽  
Joanna Miszkiewicz ◽  
Michael Feig ◽  
Joanna Trylska
Keyword(s):  
Thermal Stability ◽  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Thermal Stability Of

Thermal stability of marks gold nanoparticles: A molecular dynamics simulation

2017 ◽  
Vol 31 (07) ◽  
pp. 1741001
Author(s):  
Yanlin Jia ◽  
Siqi Li ◽  
Weihong Qi ◽  
Mingpu Wang ◽  
Zhou Li ◽  
...  
Keyword(s):  
Molecular Dynamics ◽  
Thermal Stability ◽  
Melting Temperature ◽  
Cohesive Energy ◽  
Au Nanoparticles ◽  
Mean Square Displacement ◽  
Dynamics Simulation ◽  
Structure Variation ◽  
Deformation Parameters ◽  
Thermal Stability Of

Molecular dynamics (MDs) simulations were used to explore the thermal stability of Au nanoparticles (NPs) with decahedral, cuboctahedral, icosahedral and Marks NPs. According to the calculated cohesive energy and melting temperature, the Marks NPs have a higher cohesive energy and melting temperature compared to these other shapes. The Lindemann index, radial distribution function, deformation parameters, mean square displacement and self-diffusivity have been used to characterize the structure variation during heating. This work may inspire researchers to prepare Marks NPs and apply them in different fields.

Effect of Locked Nucleic Acid Modifications on the Thermal Stability of Noncanonical DNA Structure

Biochemistry ◽  
2011 ◽  
Vol 50 (34) ◽  
pp. 7414-7425 ◽  
Author(s):  
Jhimli Bhattacharyya ◽  
Souvik Maiti ◽  
Sanjukta Muhuri ◽  
Shu-ichi Nakano ◽  
Daisuke Miyoshi ◽  
...  
Keyword(s):  
Thermal Stability ◽  
Nucleic Acid ◽  
Dna Structure ◽  
Locked Nucleic Acid ◽  
Thermal Stability Of

Peculiarities of the formation and thermal stability of barrier contacts in high-sensitivity silicon carbide detector diodes

2003 ◽  
Vol 29 (1) ◽  
pp. 22-25 ◽  
Author(s):  
N. S. Boltovets ◽  
A. V. Zorenko ◽  
V. N. Ivanov ◽  
S. I. Vlaskina ◽  
R. V. Konakova ◽  
...  
Keyword(s):  
Thermal Stability ◽  
Silicon Carbide ◽  
High Sensitivity ◽  
Thermal Stability Of

scholarly journals Predicting protein thermal stability changes upon point mutations using statistical potentials: Introducing HoTMuSiC

2016 ◽  
Author(s):  
Fabrizio Pucci ◽  
Raphaël Bourgeas ◽  
Marianne Rooman
Keyword(s):  
Thermal Stability ◽  
Melting Temperature ◽  
Point Mutations ◽  
Model Structure ◽  
Temperature Dependent ◽  
Statistical Potentials ◽  
Validation Procedure ◽  
The Impact ◽  
Fold Cross Validation ◽  
Thermal Stability Of

The accurate prediction of the impact of an amino acid substitution on the thermal stability of a protein is a central issue in protein science, and is of key relevance for the rational optimization of various bioprocesses that use enzymes in unusual conditions. Here we present one of the first computational tools to predict the change in melting temperature ΔTmupon point mutations, given the protein structure and, when available, the melting temperature Tmof the wild-type protein. The key ingredients of our model structure are standard and temperature-dependent statistical potentials, which are combined with the help of an artificial neural network. The model structure was chosen on the basis of a detailed thermodynamic analysis of the system. The parameters of the model were identified on a set of more than 1,600 mutations with experimentally measured ΔTm. The performance of our method was tested using a strict 5-fold cross-validation procedure, and was found to be significantly superior to that of competing methods. We obtained a root mean square deviation between predicted and experimental ΔTmvalues of 4.2°C that reduces to 2.9°C when ten percent outliers are removed. A webserver-based tool is freely available for non-commercial use at soft.dezyme.com.

Oligomers with structural elements of imidazolidinetrione obtained from oxamic acid and oxamide: polyurethane foams modified by structural elements of imidazolidinetrione

2015 ◽  
Vol 35 (1) ◽  
pp. 1-10 ◽  
Author(s):  
Iwona Zarzyka
Keyword(s):  
Thermal Stability ◽  
Polyurethane Foams ◽  
Thermally Stable ◽  
Structural Elements ◽  
Derivatives Of ◽  
Thermal Stability Of

Abstract Hydroxyalkyl derivatives containing oxamidoester and oxamide fragments have been separately obtained using oxamic acid, oxamide and alkylene carbonates. It has been proven that the presence of oxamidoester and oxamide structural fragments in oligomer structures is responsible for the thermal stability of these products. Rigid, thermally stable polyurethane foams were obtained with the use of oligomers with oxamidoestercarbamidoimide, carbamide, oxamidoester and oxamide groups as polyols components. The properties of these foamed materials were compared with each other and with the properties of reference foams. It was found that the polyurethane foams characterized by the best properties were obtained from hydroxypropyl derivatives of oxamide.

scholarly journals Thermal Stability of Titanium Alloy VT8M-1 with Ultrafine-Grained Structure

2020 ◽  
Vol 321 ◽  
pp. 11026
Author(s):  
A.G. Stotskiy ◽  
A.V. Polyakov ◽  
G.S. Dyakonov ◽  
I.P. Semenova
Keyword(s):  
Thermal Stability ◽  
Coarse Grained ◽  
Structural Elements ◽  
Electronic Microscopy ◽  
Rotary Swaging ◽  
Long Duration ◽  
Ultrafine Grained ◽  
Equiaxed Grains ◽  
Thermal Stability Of

The paper considers the effect of a long-duration heating at a service temperature of 450oC on VT8M-1 with a coarse-grained (CG) and ultrafine-grained (UFG) microstructure. A duplex ultrafine-grained microstructure, composed of equiaxed grains of primary α-phase and an ultrafine constituent of α- and β-phases, was processed by thermal treatment and further rotary swaging. This type of a microstructure demonstrates a best combination of strength and ductility at room temperature in comparison with the CG structure. A thermal stability of an UFG state was studied at 450°C for 50, 100, 200, 300, 400, and 500 hours. The evolution of the alloy microstructure against the duration of heating was considered by transmission electronic microscopy (TEM), scanning electronic microscopy (SEM). No increase in the alloy structural elements and strength decrease resulting from a long-term annealing (up to 500 hours) at T=450°C have been observed. This proves a high thermal stability of the UFG structure and mechanical properties of VT8M-1 processed via rotary swaging.

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