scholarly journals Identification of New Rat Bone Marrow-Derived Population of Very Small Stem Cell with Oct-4A and Nanog Expression by Flow Cytometric Platforms

2016 ◽  
Vol 2016 ◽  
pp. 1-14 ◽  
Author(s):  
Anna Labedz-Maslowska ◽  
Elzbieta Kamycka ◽  
Sylwia Bobis-Wozowicz ◽  
Zbigniew Madeja ◽  
Ewa K. Zuba-Surma
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Functional Properties ◽  
Adult Stem Cells ◽  
Tissue Injury ◽  
Population Based ◽  
Flow Cytometric ◽  
Rat Bone

Very small embryonic-like stem cells (VSELs) represent a unique rare population of adult stem cells (SCs) sharing several structural, genetic, biochemical, and functional properties with embryonic SCs and have been identified in several adult murine and human tissues. However, rat bone marrow- (BM-) derived SCs closely resembling murine or human VSELs have not been described. Thus, we employed multi-instrumental flow cytometric approach including classical and imaging cytometry and we established that newly identified population of nonhematopoietic cells expressing CD106 (VCAM-I) antigen contains SCs with very small size, expressing markers of pluripotency (Oct-4A and Nanog) on both mRNA and protein levels that indicate VSEL population. Based on our experience in both murine and human VSEL isolation procedures by fluorescence-activated cell sorting (FACS), we also optimized sorting protocol for separation of CD45−/Lin−/CD106+rat BM-derived VSELs from wild type and eGFP-expressing rats, which are often used as donor animals for cell transplantations in regenerative studiesin vivo. Thus, this is a first study identifying multiantigenic phenotype and providing sorting protocols for isolation VSELs from rat BM tissue for further examining of their functional propertiesin vitroas well as regenerative capacity in distinctin vivorat models of tissue injury.

Evidence for Vasculogenic Potential and Endothelial Differentiation of Bone-Marrow-Derived Very Small Embryonic-like Stem Cells

Blood ◽  
2014 ◽  
Vol 124 (21) ◽  
pp. 5120-5120
Author(s):  
Coralie L Guerin ◽  
Xavier Loyer ◽  
Jose Vilar ◽  
Audrey Cras ◽  
Tristan Mirault ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Conflicts Of Interest ◽  
Tissue Injury ◽  
Multipotent Stem Cells ◽  
Angiogenic Potential ◽  
Immunodeficient Mice ◽  
Positive Expression

Abstract Objective: Very small embryonic-like stem cells (VSELs) are multipotent stem cells localized in adult bone marrow (BM) that may be mobilized into peripheral blood (PB) in response to tissue injury. We aimed to quantify VSELs in BM and PB of patients with critical limb ischemia (CLI) and to test their angiogenic potential in vitro as well as their therapeutic capacity in mouse model of CLI. Approach and Results: We isolated BM VSELs from patients with CLI and studied their potential to differentiate into vascular lineages. Flow and imaging cytometry showed that VSEL counts were lower in BM (p<0.001) and higher (p<0.001) in PB from CLI patients compared to healthy controls, suggesting that ischemia may trigger VSELs mobilization in this patient population. Sorted BM-VSELs cultured in angiogenic media acquired a mesenchymal phenotype (CD90+, Thy-1 gene positive expression). VSEL-derived cells had a pattern of secretion similar to that of endothelial progenitor cells, as they released low levels of VEGF-A and inflammatory cytokines. Noteworthy, VSELs triggered post-ischemic revascularization in immunodeficient mice (p<0.05 vs PBS treatment), and acquired an endothelial phenotype either in vitro when cultured in the presence of VEGF-B (Cdh-5 gene positive expression), or in vivo in Matrigel implants (human CD31+ staining in neo-vessels from plug sections). Conclusions: VSELs are a potential new source of therapeutic cells that may give rise to cells of the endothelial lineage in humans. Disclosures No relevant conflicts of interest to declare.

A subset of human rapidly self-renewing marrow stromal cells preferentially engraft in mice

Blood ◽  
2006 ◽  
Vol 107 (5) ◽  
pp. 2153-2161 ◽  
Author(s):  
Ryang Hwa Lee ◽  
Shu Ching Hsu ◽  
James Munoz ◽  
Jin Sup Jung ◽  
Na Rea Lee ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Progenitor Cells ◽  
Stromal Cells ◽  
Adult Stem Cells ◽  
Tissue Injury ◽  
Marrow Stromal Cells ◽  
Immunodeficient Mice ◽  
Adult Mice

Controversies have arisen as to whether adult stem cells or progenitor cells from bone marrow can engraft into nonhematopoietic tissues in vivo. To resolve some of the controversies, we developed a highly sensitive polymerase chain reaction-based single nucleotide polymorphism (PCR-SNP) assay for competitive engraftment of mixtures of stem/progenitor cells. We used the assay to follow engraftment in immunodeficient mice of subpopulations of the stem/progenitor cells from human bone marrow referred to as either mesenchymal stem cells or marrow stromal cells (MSCs). The engraftment into adult mice without induced tissue injury was low and variable, but there was preferential engraftment of a subpopulation of rapidly self-renewing MSCs (RS-MSCs) compared with a subpopulation of slowly renewing MSCs (SR-MSCs). After intravenous infusion, there was a tendency for the cells to engraft into the hippocampal region that was previously designated a “vascular niche.” Migration assays suggested that preferential engraftment of RS-MSCs was in part explained by their expression of CXCR4 and CX3R1, the receptors for SDF-1 and fractalkine.

Bone-marrow-derived very small embryonic-like stem cells in patients with critical leg ischaemia: evidence of vasculogenic potential

2015 ◽  
Vol 113 (05) ◽  
pp. 1084-1094 ◽  
Author(s):  
Coralie L. Guerin ◽  
Xavier Loyer ◽  
José Vilar ◽  
Audrey Cras ◽  
Tristan Mirault ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Tissue Injury ◽  
Critical Limb Ischaemia ◽  
Limb Ischaemia ◽  
Multipotent Stem Cells ◽  
Immunodeficient Mice ◽  
Positive Expression

SummaryVery small embryonic-like stem cells (VSELs) are multipotent stem cells localised in adult bone marrow (BM) that may be mobilised into peripheral blood (PB) in response to tissue injury. We aimed to quantify VSELs in BM and PB of patients with critical limb ischaemia (CLI) and to test their angiogenic potential in vitro as well as their therapeutic capacity in mouse model of CLI. We isolated BM VSELs from patients with CLI and studied their potential to differentiate into vascular lineages. Flow and imaging cytometry showed that VSEL counts were lower in BM (p< 0.001) and higher (p< 0.001) in PB from CLI patients compared to healthy controls, suggesting that ischaemia may trigger VSELs mobilisation in this patient population. Sorted BM-VSELs cultured in angiogenic media acquired a mesenchymal phenotype (CD90+, Thy-1 gene positive expression). VSEL-derived cells had a pattern of secretion similar to that of endothelial progenitor cells, as they released low levels of VEGF-A and inflammatory cytokines. Noteworthy, VSELs triggered post-ischaemic revascularisation in immunodeficient mice (p< 0.05 vs PBS treatment), and acquired an endothelial phenotype either in vitro when cultured in the presence of VEGF-B (Cdh-5 gene positive expression), or in vivo in Matrigel implants (human CD31+ staining in neo-vessels from plug sections). In conclusion, VSELs are a potential new source of therapeutic cells that may give rise to cells of the endothelial lineage in humans.

Reciprocal expression of CD38 and CD34 by adult murine hematopoietic stem cells

Blood ◽  
2001 ◽  
Vol 97 (9) ◽  
pp. 2618-2624 ◽  
Author(s):  
Fumihito Tajima ◽  
Takao Deguchi ◽  
Joseph H. Laver ◽  
Haiqun Zeng ◽  
Makio Ogawa
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Steady State ◽  
Adult Stem Cells ◽  
Human Stem Cells ◽  
Hematopoietic Stem ◽  
Reciprocal Expression

Abstract The effects of activation of adult murine stem cells on their expression of CD38 were studied using a murine transplantation model. First, the published finding that the majority of long-term engrafting cells from normal adult steady-state marrow are CD38+ was confirmed. Next, it was determined that the majority of stem cells activated in vivo by injection of 5-fluorouracil (5-FU) or mobilized by granulocyte colony-stimulating factor are CD38−. Stem cells that were activated in culture with interleukin-11 and steel factor were also CD38−. Previous studies have shown that expression of CD34 by adult stem cells is also modulated by in vivo or in vitro activation. To determine whether there is reciprocal expression of CD38 and CD34, 4 populations of post–5-FU marrow cells were analyzed. The majority of the stem cells were in the CD38−CD34+ fraction. However, secondary transplantation experiments indicated that when the bone marrow reaches steady state, the majority of the stem cells become CD38+CD34−. In addition, the minority populations of CD34+ stem cells that occur in steady-state bone marrow are CD38−. This reversible and reciprocal expression of CD38 and CD34 by murine stem cells may have implications for the phenotypes of human stem cells.

506 HEPATOGENIC DIFFERENTIATION OF RAT BONE MARROW MESENCHYMAL STEM CELLS IN VITRO AND IN VIVO

2008 ◽  
Vol 48 ◽  
pp. S191
Author(s):  
S.H. Bae ◽  
J.Y. Choi ◽  
S.K. Yoon ◽  
K.H. Yoon ◽  
B.S. Kim ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Hepatogenic Differentiation ◽  
Marrow Mesenchymal Stem Cells ◽  
Rat Bone

Plasticity of marrow-derived stem cells

Blood ◽  
2003 ◽  
Vol 102 (10) ◽  
pp. 3483-3493 ◽  
Author(s):  
Erica L. Herzog ◽  
Li Chai ◽  
Diane S. Krause
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Tissue Injury ◽  
Adult Stem Cell ◽  
Neural Cells ◽  
Cell Plasticity ◽  
Hematopoietic Stem ◽  
The Subject

AbstractBone marrow (BM) contains hematopoietic stem cells (HSCs), which differentiate into every type of mature blood cell; endothelial cell progenitors; and marrow stromal cells, also called mesenchymal stem cells (MSCs), which can differentiate into mature cells of multiple mesenchymal tissues including fat, bone, and cartilage. Recent findings indicate that adult BM also contains cells that can differentiate into additional mature, nonhematopoietic cells of multiple tissues including epithelial cells of the liver, kidney, lung, skin, gastrointestinal (GI) tract, and myocytes of heart and skeletal muscle. Experimental results obtained in vitro and in vivo are the subject of this review. The emphasis is on how these experiments were performed and under what conditions differentiation from bone marrow to epithelial and neural cells occurs. Questions arise regarding whether tissue injury is necessary for this differentiation and the mechanisms by which it occurs. We also consider which bone marrow subpopulations are capable of this differentiation. Only after we have a better understanding of the mechanisms involved and of the cells required for this differentiation will we be able to fully harness adult stem cell plasticity for clinical purposes. (Blood. 2003; 102:3483-3493)

Dosage and Passage Dependent Neuroprotective Effects of Exosomes Derived from Rat Bone Marrow Mesenchymal Stem Cells: An In Vitro Analysis

2018 ◽  
Vol 18 ◽  
Author(s):  
Chaitra Venugopal ◽  
Christopher Shamir ◽  
Sivapriya Senthilkumar ◽  
Janitri Venkatachala Babu ◽  
Peedikayil Kurien Sonu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Neuroprotective Effects ◽  
Marrow Mesenchymal Stem Cells ◽  
Vitro Analysis ◽  
In Vitro Analysis ◽  
Rat Bone
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