scholarly journals A Comparison of Conventional Collagen Sponge and Collagen-Gelatin Sponge in Wound Healing

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Chizuru Jinno ◽  
Naoki Morimoto ◽  
Ran Ito ◽  
Michiharu Sakamoto ◽  
Shuichi Ogino ◽  
...  
Keyword(s):  
Wound Healing ◽  
Tissue Regeneration ◽  
Saline Solution ◽  
Collagen Sponge ◽  
Gelatin Sponge ◽  
Fibroblast Growth ◽  
Wound Area ◽  
Thickness Skin ◽  
Tissue Area ◽  
Tissue Specimens

The objective of this study was to compare the effectiveness of the collagen-gelatin sponge (CGS) with that of the collagen sponge (CS) in dermis-like tissue regeneration. CGS, which achieves the sustained release of basic fibroblast growth factor (bFGF), is a promising material in wound healing. In the present study, we evaluated and compared CGSs and conventional CSs. We prepared 8 mm full-thickness skin defects on the backs of rats. Either CGSs or CSs were impregnated with normal saline solution (NSS) or 7 μg/cm2of bFGF solution and implanted into the defects. At 1 and 2 weeks after implantation, tissue specimens were obtained from the rats of each group (n=3, totaln=24). The wound area, neoepithelial length, dermis-like tissue area, and the number and area of capillaries were evaluated at 1 and 2 weeks after implantation. There were no significant differences in the CGS without bFGF and CS groups. Significant improvements were observed in the neoepithelial length, the dermis-like tissue area, and the number of newly formed capillaries in the group of rats that received CGSs impregnated with bFGF. The effects on epithelialization, granulation, and vascularization of wound healing demonstrated that, as a scaffold, CGSs are equal or superior to conventional CSs.

scholarly journals Solanum incanum extract enhances wound healing and tissue regeneration in burn mice model

2019 ◽  
Vol 14 (2) ◽  
pp. 101-106
Author(s):  
Zainab Qureshi ◽  
Taous Khan ◽  
Abdul Jabbar Shah ◽  
Fazli Wahid
Keyword(s):  
Wound Healing ◽  
Tissue Regeneration ◽  
Histological Analysis ◽  
Negative Control ◽  
Wound Contraction ◽  
Mice Model ◽  
Wound Area ◽  
Solanum Incanum ◽  
Partial Thickness ◽  
Partial Thickness Burn

This study was conducted to evaluate the topical efficacy of Solanum incanum for the treatment of partial-thickness burn in mice model. Mice were treated with topical ointment of S. incanum three times daily for 14 days. The wound healing was observed through wound contraction and histological parameters. The group treated with S. incanum ointment showed 81% reduction in wound area as compared to negative control where wound area reduced to 22%. The histological analysis further confirmed that ointment favors the tissue regeneration and reepithelization thus heal wound rapidly as com-pared to other groups. In conclusion, S. incanum extract enhances wound healing and tissue regeneration.

scholarly journals Full-thickness skin reconstruction with basic fibroblast growth factor-impregnated collagen-gelatin sponge

2019 ◽  
Vol 11 ◽  
pp. 81-87 ◽  
Author(s):  
Hajime Matsumine ◽  
Hiroshi Fujimaki ◽  
Mika Takagi ◽  
Satoko Mori ◽  
Tomohiro Iwata ◽  
...  
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Gelatin Sponge ◽  
Fibroblast Growth ◽  
Full Thickness ◽  
Full Thickness Skin ◽  
Thickness Skin ◽  
Skin Reconstruction

scholarly journals Evaluation of Indonesian and two types of Japanese honey on the acceleration of cutaneous wound healing: an experimental study in mice

2019 ◽  
Vol 2 (2) ◽  
pp. 80-88
Author(s):  
Haryanto Haryanto ◽  
Kanae Mukai ◽  
Nakajima Yukari ◽  
Mayumi Okuwa ◽  
Cau Kim Jiu ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Control Group ◽  
Wound Healing Process ◽  
Wound Area ◽  
Hydrocolloid Dressing ◽  
Thickness Skin ◽  
Microscopic Evaluation ◽  
Acacia Honey ◽  
Macroscopic Findings

The purpose of this study is to evaluate and clarify the effect of Indonesian honey, two types of Japanese honey and hydrocolloid dressing on wound healing process. Four groups of male mice were treated to produce two circular full-thickness skin wounds on the dorsum. They were then randomly allocated to receive daily Indonesian honey, Japanese honey (Buckwheat and Acacia honey) or hydrocolloid dressing as a control for treatment application. Macroscopic findings were observed from day 0 to 14 after wounding. Microscopic evaluation was assessed using qualitative analysis. The ratios of wound areas for honey groups on day 3 and 7 were smaller than those of the control group. Wound areas of honey groups gradually decreased to almost the same wound area as the control group on day 14, while the wound area of the control group peaked on day 5 and rapidly decreased until day 14. Microscopic finding that Indonesian honey was different with Japanese honey especially Buckwheat honey.

scholarly journals The Effect of Adipose-Derived Stem Cells on Wound Healing: Comparison of Methods of Application

2019 ◽  
Vol 2019 ◽  
pp. 1-8 ◽  
Author(s):  
Hyeonwoo Kim ◽  
Mi Ri Hyun ◽  
Sang Wha Kim
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Imaging System ◽  
Medical Problem ◽  
Adipose Derived Stem Cells ◽  
Control Groups ◽  
Impaired Wound Healing ◽  
Wound Area ◽  
Cell Based Therapy ◽  
Thickness Skin

Impaired wound healing is a significant medical problem. Recently, cell-based therapy focused on stem cells has been developed to overcome the challenges of defective wound healing. In this study, we aimed to evaluate the effectiveness of adipose-derived stem cells (ASCs) in promoting wound healing, using different techniques for administering them. Dorsal full-thickness skin defects (1×1 cm) were created in three groups of mice that received intravenous ASCs by intravenous injection, intramuscular injection, and topical application, respectively. Three control groups received saline in the same ways. Wound healing was assessed clinically, wounds were examined histologically, and GFP-labelled ASCs were detected with an IVIS imaging system. The results revealed that ASCs accelerated wound healing independent of their mode of administration. Histological examination showed that the ASCs accelerated reepithelialization, and IVIS analysis indicated that many ASCs were present in the wound area and disappeared after wound healing.

scholarly journals Expression of fibroblast growth factors and their receptors during full-thickness skin wound healing in young and aged mice

2005 ◽  
Vol 186 (2) ◽  
pp. 273-289 ◽  
Author(s):  
Akiko Komi-Kuramochi ◽  
Mitsuko Kawano ◽  
Yuko Oda ◽  
Masahiro Asada ◽  
Masashi Suzuki ◽  
...  
Keyword(s):  
Wound Healing ◽  
Growth Factors ◽  
Growth Factor ◽  
Fibroblast Growth Factors ◽  
Fibroblast Growth ◽  
Full Thickness ◽  
Healthy Skin ◽  
Aged Mice ◽  
Full Thickness Skin ◽  
Thickness Skin

The highly ordered process of wound healing involves the coordinated regulation of cell proliferation and migration and tissue remodeling, predominantly by polypeptide growth factors. Consequently, the slowing of wound healing that occurs in the aged may be related to changes in the activity of these various regulatory factors. To gain additional insight into these issues, we quantified the absolute copy numbers of mRNAs encoding all the fibroblast growth factors (FGFs), their receptors (FGFRs) and two other growth factors in the dorsal skin of young and aged mice during the healing of full-thickness skin excisional wounds. In young adult mice (8 weeks old), FGF7, FGF10 and FGF22 mRNAs were all strongly expressed in healthy skin, and levels of FGF7 and 10 but not 22 increased 2- to 3.5-fold over differing time courses after wounding. The levels of FGF9, 16, 18 and especially 23 mRNAs were moderate or low in healthy skin but increased 2- to 33-fold after wounding. Among the four FGFRs, expression of only FGFR1 mRNA was augmented during wound healing. Expression of transforming growth factor-β and hepatocyte growth factor was also high in healthy skin and was upregulated during healing. Notably, in aged mice (35 weeks old), where healing proceeded more slowly than in the young, both the basal and wound-induced mRNA expression of most of these genes was reduced. While these results confirm the established notion that FGFR2 IIIB ligands (FGF7 and FGF10) are important for wound healing, they also suggest that decreased expression of multiple FGF ligands contributes to the slowing of wound healing in aged mice and indicate the potential importance of further study of the involvement of FGF9, 16, 18 and 23 in the wound healing process.

scholarly journals Effects of Three Types of Japanese Honey on Full-Thickness Wound in Mice

2013 ◽  
Vol 2013 ◽  
pp. 1-11 ◽  
Author(s):  
Yukari Nakajima ◽  
Yuki Nakano ◽  
Sono Fuwano ◽  
Natsumi Hayashi ◽  
Yukiho Hiratoko ◽  
...  
Keyword(s):  
Wound Healing ◽  
Combined Treatment ◽  
Full Thickness ◽  
Wound Area ◽  
Proliferative Phase ◽  
Inflammatory Phase ◽  
Hydrocolloid Dressing ◽  
Thickness Skin ◽  
Full Thickness Wound ◽  
Buckwheat Flour

Although many previous studies reported that honey promotes wound healing, no study has examined the effects of Japanese honey. The aim of this study was to investigate the effects of three types of Japanese honey, Acacia, Buckwheat flour, and Chinese milk vetch honey, on wound healing in comparison with hydrocolloid dressing. Circular full-thickness skin wounds were produced on male mice. Japanese honey or hydrocolloid dressing was applied daily to the mice for 14 days. The ratio of wound area for the hydrocolloid dressing group increased initially in the inflammatory and early proliferative phases and then decreased rapidly to heal with scarring. However, the ratios of wound area for the Japanese honey groups decreased in the inflammatory phase, increased in the proliferative phase, and decreased in the proliferative phase, and some wounds were not completely covered with new epithelium. These findings indicate that using Japanese honey alone has limited benefit, but since it reduces wound size in the inflammatory phase, it is possible to apply a combined treatment in which Japanese honey is applied only in the inflammatory phase, followed by hydrocolloid dressing from the proliferative phase, which would effectively contract the wound.

scholarly journals Effect of Propolis on Experimental Cutaneous Wound Healing in Dogs

2015 ◽  
Vol 2015 ◽  
pp. 1-4 ◽  
Author(s):  
Ashraf M. Abu-Seida
Keyword(s):  
Wound Healing ◽  
Positive Impact ◽  
Control Group ◽  
Cutaneous Wound Healing ◽  
Wound Area ◽  
Cutaneous Wound ◽  
Thickness Skin ◽  
Group 10 ◽  
Complete Wound Healing ◽  
Aseptic Conditions

This study evaluates clinically the effect of propolis paste on healing of cutaneous wound in dogs. Under general anesthesia and complete aseptic conditions, two full thickness skin wounds (3 cm diameter) were created in each side of the chest in five dogs, one dorsal and one ventral, with 10 cm between them. These wounds were randomly allocated into two groups, control group (10 wounds) and propolis group (10 wounds). Both groups were represented in each dog. The wounds were cleaned with normal saline solution and dressed with macrogol ointment in control group and propolis paste in propolis group, twice daily till complete wound healing. Measurement of the wound area (cm2) was monitored planimetrically at 0, 7, 14, 21, 28, and 35 days after injury. The data were analyzed statistically. The results revealed a significant reduction in the wound surface area in the propolis group after 14 and 21 days compared to control group. The wound reepithelization, contraction, and total wound healing were faster in propolis group than in control group during five weeks of study. In conclusion, propolis paste has a positive impact on cutaneous wound healing and it may be suggested for treating various types of wounds in animals.

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