scholarly journals Colonic and Hepatic Modulation by Lipoic Acid and/or N-Acetylcysteine Supplementation in Mild Ulcerative Colitis Induced by Dextran Sodium Sulfate in Rats

2016 ◽  
Vol 2016 ◽  
pp. 1-18 ◽  
Author(s):  
Fabiana Andréa Moura ◽  
Kívia Queiroz de Andrade ◽  
Orlando Roberto Pimentel de Araújo ◽  
Valéria Nunes-Souza ◽  
Juliana Célia de Farias Santos ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Lipoic Acid ◽  
Sodium Sulfate ◽  
High Performance ◽  
Dextran Sodium Sulfate ◽  
Rat Tissues ◽  
Myeloperoxidase Activity ◽  
Necrosis Factor Alpha ◽  
Anti Inflammatory ◽  
Pretreatment Phase

Lipoic acid (LA) andN-acetylcysteine (NAC) are antioxidant and anti-inflammatory agents that have not yet been tested on mild ulcerative colitis (UC). This study aims to evaluate the action of LA and/or NAC, on oxidative stress and inflammation markers in colonic and hepatic rat tissues with mild UC, induced by dextran sodium sulfate (DSS) (2% w/v). LA and/or NAC (100 mg·kg·day−1, each) were given, once a day, in the diet, in a pretreatment phase (7 days) and during UC induction (5 days). Colitis induction was confirmed by histological and biochemical analyses (high performance liquid chromatography, spectrophotometry, and Multiplex®). A redox imbalance occurred before an immunological disruption in the colon. NAC led to a decrease in hydrogen peroxide (H2O2), malondialdehyde (MDA) levels, and myeloperoxidase activity. In the liver, DSS did not cause damage but treatments with both antioxidants were potentially harmful, with LA increasing MDA and LA + NAC increasing H2O2, tumor necrosis factor alpha, interferon gamma, and transaminases. In summary, NAC exhibited the highest colonic antioxidant and anti-inflammatory activity, while LA + NAC caused hepatic damage.

scholarly journals THE EFFECT OF MAHKOTA DEWA (PHALERIA MACROCARPA) (SCHEFF.) FRUIT PERICARP EXTRACT ON INOS IN MICE COLON INTERMITTENTLY-INDUCED BY DEXTRAN SODIUM SULFATE

2017 ◽  
Vol 10 (12) ◽  
pp. 309
Author(s):  
Kusmardi Kusmardi ◽  
Dilla Shavera ◽  
Ari Estuningtyas ◽  
Aryo Tedjo ◽  
Bambang Priyosoeryanto
Keyword(s):  
Sodium Sulfate ◽  
Total Yield ◽  
Ethanolic Extract ◽  
Dextran Sodium Sulfate ◽  
Inflammatory Activity ◽  
Fruit Pericarp ◽  
Phaleria Macrocarpa ◽  
Anti Inflammatory ◽  
Oxide Synthase ◽  
Different Doses

  Objective: The objective of this research was to investigate the anti-inflammatory effect of Mahkota Dewa fruit pericarp extract (Phaleria macrocarpa) on inducible nitric oxide synthase (iNOS) in mice colon induced by dextran sodium sulfate (DSS).Method: The simplisia of P. macrocarpa pericarp was weighed (1000 g) and extracted by maceration process. The total yield of the ethanolic extract was 26.43%. Phytochemical screening was carried out for the detection of the phytoconstituents by simple qualitative methods. The anti-inflammatory activity was performed by DSS-induced colitis model through assessment of hematoxylin-eosin staining and expression of iNOS by immunohistochemistry assay at four different doses, i.e., 650, 1250, 2500, and 5000 mg/kg. Swiss Webster male mice weighing 25-30 g were used for the study.Results: Inflammation score in dose 625, 1250, 2500, and 5000 mg/kg were 1.63, 1.43, 1.32, and 2.20, respectively. This result is significantly different (p=0.008) with DSS group that was 4.37. The results of iNOS optical density score in dose 625, 1250, 2500, and 5000 mg/kg were 1.21, 1.119, 1.22, and 1.37, respectively. This result was significantly different (p=0.000) with DSS group that was 2.24.Conclusion: Pericarp extract of P. macrocarpa fruit exhibited anti-inflammatory activity in the experimental model shown by suppressing the expression of inflammatory cell and iNOS. 

The Role of SPARC in Human Ulcerative Colitis and Murine Dextran Sodium Sulfate-Induced Colitis

2011 ◽  
Vol 140 (5) ◽  
pp. S-837
Author(s):  
Tomohisa Takagi ◽  
Yuji Naito ◽  
Wataru Aoi ◽  
Ryusuke Horie ◽  
Kazuhiko Uchiyama ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Sodium Sulfate ◽  
Dextran Sodium Sulfate

scholarly journals Anti-inflammatory Bifidobacterium strains prevent dextran sodium sulfate induced colitis and associated gut microbial dysbiosis in mice

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Shashank Singh ◽  
Ruchika Bhatia ◽  
Pragyanshu Khare ◽  
Shikha Sharma ◽  
Sivasubramanian Rajarammohan ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Sodium Sulfate ◽  
Activity Index ◽  
Disease Activity Index ◽  
Dextran Sodium Sulfate ◽  
Raw 264.7 Cells ◽  
Spleen Weight ◽  
Lipocalin 2 ◽  
Weight Percentage ◽  
Microbial Dysbiosis

Abstract Crohn’s and ulcerative colitis are common inflammatory conditions associated with Inflammatory bowel disease. Owing to the importance of diet based approaches for the prevention of inflammatory gut conditions, the present study was aimed to screen the human isolates of Bifidobacterium strains based on their ability to reduce LPS-induced inflammation in murine macrophage (RAW 264.7) cells and to evaluate prioritized strains for their preventive efficacy against ulcerative colitis in mice. Twelve out of 25 isolated strains reduced the production of LPS-induced nitric oxide and inflammatory cytokines. Furthermore, three strains, B. longum Bif10, B. breve Bif11, and B. longum Bif16 conferred protection against dextran sodium sulfate induced colitis in mice. The three strains prevented shortening of colon, spleen weight, percentage body weight change and disease activity index relative to colitis mice. Lower levels of Lipocalin-2, TNF-α, IL-1β and IL-6 and improved SCFA levels were observed in Bifidobacterium supplemented mice relative to DSS counterparts. Bacterial composition of B. longum Bif10 and B. breve Bif11 fed mice was partly similar to the normal mice, while DSS and B. longum Bif16 supplemented mice showed deleterious alterations. At the genus level, Bifidobacterium supplementation inhibited the abundances of pathobionts such as Haemophilus, Klebsiella and Lachnospira there by conferring protection.

Therapeutic Effects of Ecabet Sodium, an Antiulcer Drug, on Dextran Sodium Sulfate–Induced Ulcerative Colitis in Rats

2005 ◽  
Vol 50 (5) ◽  
pp. 922-927 ◽  
Author(s):  
Tsunehisa Noto ◽  
Hiroshi Yamada ◽  
Takashi Inui ◽  
Kayoko Okuyama ◽  
Ayako Watanable ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Sodium Sulfate ◽  
Dextran Sodium Sulfate ◽  
Therapeutic Effects ◽  
Antiulcer Drug ◽  
Ecabet Sodium

Effects of a β-type glycosidic polysaccharide from Flammulina velutipes on anti-inflammation and gut microbiota modulation in colitis mice

2020 ◽  
Vol 11 (5) ◽  
pp. 4259-4274
Author(s):  
Ruiqiu Zhao ◽  
Yang Ji ◽  
Xin Chen ◽  
Anxiang Su ◽  
Gaoxing Ma ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Gut Microbiota ◽  
Sodium Sulfate ◽  
Dextran Sodium Sulfate ◽  
Flammulina Velutipes ◽  
Anti Inflammation

Using the Flammulina velutipes polysaccharide (FVP) extracted from our previous study, herein, we investigated the improvement of this β-type glycosidic polysaccharide in alleviating dextran sodium sulfate-induced ulcerative colitis (UC) in mice.

scholarly journals Andrographolide Derivative AL-1 Ameliorates Dextran Sodium Sulfate-Induced Murine Colitis by Inhibiting NF-κB and MAPK Signaling Pathways

2019 ◽  
Vol 2019 ◽  
pp. 1-18 ◽  
Author(s):  
Mei Jing ◽  
Yuqiang Wang ◽  
Lipeng Xu
Keyword(s):  
Signaling Pathways ◽  
Inflammatory Cytokines ◽  
Sodium Sulfate ◽  
Mapk Signaling ◽  
Dextran Sodium Sulfate ◽  
Myeloperoxidase Activity ◽  
Altered Expression ◽  
Murine Colitis ◽  
Mapk Signaling Pathways

Trinitrobenzenesulfonic acid (TNBS) and dextran sodium sulfate (DSS) are commonly used to induce experimental murine ulcerative colitis (UC). Our recent study has demonstrated that a novel andrographolide derivative, AL-1, ameliorated TNBS-induced colitis in mice. However, the effect of AL-1 on DSS-induced murine colitis and the underlying mechanisms are yet unknown. In the present study, we aimed to investigate the therapeutic potential of AL-1 against DSS-induced UC in mice and to define its mechanisms of action. Oral administration of AL-1 attenuated body weight loss, reduced colon length shortening, lowered the disease activity index score, and alleviated colon histological damage. AL-1 significantly inhibited myeloperoxidase activity and suppressed immune inflammatory responses in colonic tissues. Moreover, AL-1 reversed DSS-altered expression of inflammatory cytokines in DSS-induced colitis mice. Importantly, the efficacy of 45 mg/kg of AL-1 was higher than that of 100 mg/kg of the positive control drugs 5-aminosalicylic acid and mesalazine. AL-1 decreased lipopolysaccharide-induced generation of reactive oxygen species and nitric oxide in cultured macrophages in vitro; it also reversed the altered expression of inflammatory cytokines. In both in vivo and in vitro studies, Western blot analysis revealed that AL-1 reduced the expression of phosphorylated NF-κB p65 and IκBα, downregulated the expression of iNOS and COX-2, and attenuated the expression of phosphorylated p38 mitogen-activated protein kinase (MAPK), ERK, and JNK. In conclusion, AL-1 alleviated DSS-induced murine colitis by inhibiting activation of the NF-κB and MAPK signaling pathways. Our data suggest that AL-1 could be a potential new treatment for UC.

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