scholarly journals Evaluating the Functionality of Conjunctiva Using a Rabbit Dry Eye Model

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Yuan Ning ◽  
Dhruva Bhattacharya ◽  
Richard E. Jones ◽  
Fangkun Zhao ◽  
Rongji Chen ◽  
...  
Keyword(s):  
Dry Eye ◽  
Nictitating Membrane ◽  
Impression Cytology ◽  
Schirmer Test ◽  
Eye Model ◽  
Tear Production ◽  
Harderian Glands ◽  
New Zealand White Rabbits ◽  
Necrosis Factor ◽  
Epithelial Sodium Channel Enac

Purpose.To assess the conjunctival functionality in a rabbit dry eye (DE) model.Methods.Nictitating membrane, lacrimal and Harderian glands were surgically excised from male New Zealand white rabbits using minimally invasive surgery. Fluorescein/rose Bengal staining of ocular surface (OS) and Schirmer test were done before (BE) and after excision (AE). The expression of interleukin- (IL-) 1β, tumor necrosis factor- (TNF-)α, and MUC5AC proteins were estimated by immunoblotting from conjunctival impression cytology specimens. MUC5AC mRNA was quantified as well. The effect of epithelial sodium channel (ENaC) blockers on tear production and potential differences (PD) of OS were assessed under anesthesia in rabbits with and without surgery.Results.Increase in corneal and conjunctival staining was observed 1 month AE compared to BE. Schirmer tests failed to show decrease in tear production. Elevated IL-1β, and TNF-α, 1 month AE indicated inflammation. MUC5AC expression was elevated 1 month AE. ENaC blockers did not improve tear production in rabbit eyes AE but characteristic changes in PD were observed in rabbits with surgery.Conclusions.DE biomarkers are important tools for OS assessment and MUC5AC expression is elevated in rabbit DE. PD measurement revealed significant electrophysiological changes in rabbits with surgery.

scholarly journals Comparison between two cyclooxygenase inhibitors in an experimental dry eye model in albino rabbits

2008 ◽  
Vol 58 (2) ◽  
pp. 163-173 ◽  
Author(s):  
Amany EL-Shazly ◽  
Amal EL-Gohary ◽  
Laila EL-Shazly ◽  
Ghada EL-Hossary
Keyword(s):  
Dry Eye ◽  
Cyclooxygenase Inhibitors ◽  
Eye Drops ◽  
Atropine Sulphate ◽  
Schirmer Test ◽  
Eye Model ◽  
Cox Inhibitor ◽  
Systemic Side Effects ◽  
Cox 2 ◽  
Corneal Staining

Comparison between two cyclooxygenase inhibitors in an experimental dry eye model in albino rabbitsThe purpose of this study was to compare the topical anti-inflammatory effects of the nonselective cyclooxygenase (COX) inhibitor, ketorolac, with the selective COX-2 inhibitor, nimesulide, in an animal model of dry eye in albino rabbits. All animals were examined by the Schirmer test, tear break-up time (TBUT) and fluorescein corneal staining test. Dry eye model showed significant reduction in tear volume, TBUT, corneal staining and histopathological signs of dryness and inflammation. On treating dry eye model with nimesulide 0.1% eye drops and ketorolac 0.5% eye drops, there were improvements in Schirmer test values, TBUT and fluorescein corneal staining and histopathologically reduced inflammatory reaction, with signs of healing and regeneration. Both nimesulide and ketorolac ameliorate atropine sulphate induced dry eye in albino rabbits. The use of selective COX-2 inhibitor, nimesulide, is preferred to avoid local and systemic side effects which may occur with the use of the nonselective COX inhibitor, ketorolac.

Evaluation of Ocular Surface Changes in a Rabbit Dry Eye Model Using a Modified Impression Cytology Technique

2007 ◽  
Vol 32 (4) ◽  
pp. 301-307 ◽  
Author(s):  
Dilek Dursun Altinors ◽  
Simge Bozbeyoglu ◽  
Gulten Karabay ◽  
Yonca A. Akova
Keyword(s):  
Dry Eye ◽  
Ocular Surface ◽  
Impression Cytology ◽  
Eye Model ◽  
Surface Changes

scholarly journals Alteration of corneal biomechanical properties in patients with dry eye disease

PLoS ONE ◽  
2021 ◽  
Vol 16 (7) ◽  
pp. e0254442
Author(s):  
Vannarut Satitpitakul ◽  
Parichart Taweekitikul ◽  
Vilavun Puangsricharern ◽  
Ngamjit Kasetsuwan ◽  
Usanee Reinprayoon ◽  
...  
Keyword(s):  
Regression Analysis ◽  
Dry Eye ◽  
Linear Regression Analysis ◽  
Corneal Thickness ◽  
Schirmer Test ◽  
Corvis St ◽  
Tear Production ◽  
Biomechanical Parameters ◽  
Corneal Staining ◽  
Symptoms And Signs

Purpose To evaluate the association between symptoms and signs of dry eye diseases (DED) with corneal biomechanical parameters. Methods This cross-sectional study enrolled 81 participants without history of ocular hypertension, glaucoma, keratoconus, corneal edema, contact lens use, diabetes, and ocular surgery. All participants were evaluated for symptoms and signs of DED using OSDI questionnaire, tear film break-up time (TBUT), conjunctival and corneal staining (NEI grading) and Schirmer test. Corneal biomechanical parameters were obtained using Corvis ST. Mixed-effects linear regression analysis was used to determine the association between symptoms and signs of DED with corneal biomechanical parameters. Difference in corneal biomechanical parameter between participants with low (Schirmer value ≤10 mm; LT group) and normal (Schirmer value >10mm; NT group) tear production was analyzed using ANCOVA test. Results The median OSDI scores, TBUT, conjunctival and corneal staining scores as well as Schirmer test were 13±16.5 (range; 0–77), 5.3±4.2 seconds (range; 1.3–11), 0±1 (range; 0–4), 0±2 (ranges; 0–9) and 16±14 mm (range; 0–45) respectively. Regression analysis adjusted with participants’ refraction, intraocular pressure, and central corneal thickness showed that OSDI had a negative association with highest concavity radius (P = 0.02). The association between DED signs and corneal biomechanical parameters were found between conjunctival staining scores with second applanation velocity (A2V, P = 0.04), corneal staining scores with second applanation length (A2L, P = 0.01), Schirmer test with first applanation time (A1T, P = 0.04) and first applanation velocity (P = 0.01). In subgroup analysis, there was no difference in corneal biomechanical parameters between participants with low and normal tear production (P>0.05). The associations were found between OSDI with time to highest concavity (P<0.01) and highest displacement of corneal apex (HC-DA, P = 0.04), conjunctival staining scores with A2L (P = 0.01) and A2V (P<0.01) in LT group, and Schirmer test with A1T (P = 0.02) and HC-DA (P = 0.03), corneal staining scores with A2L (P<0.01) in NT group. Conclusions According to in vivo observation with Corvis ST, patients with DED showed more compliant corneas. The increase in dry eye severity was associated with the worsening of corneal biomechanics in both patients with low and normal tear production.

Surface Mucin Changes in the Rabbit Dry Eye Model using a modified Impression Cytology Technique

2005 ◽  
Vol 3 ◽  
pp. S59
Author(s):  
Dilek Dursun ◽  
Simge Bozbeyoğlu ◽  
Gülten Karabay ◽  
Banu Bilezikci ◽  
Yonca A. Akova ◽  
...  
Keyword(s):  
Dry Eye ◽  
Impression Cytology ◽  
Eye Model

scholarly journals Effect of persimmon leaves (Diospyros kaki) on goblet cell density and inflammation in experimental dry eye model

2020 ◽  
Vol 63 (1) ◽  
Author(s):  
Kyung-A Kim ◽  
Chung Hyun Lee ◽  
Tae Kyeom Kang ◽  
Sung Jae Yang ◽  
Chang Yong Lee ◽  
...  
Keyword(s):  
Cell Density ◽  
Dry Eye ◽  
Goblet Cell ◽  
Diospyros Kaki ◽  
Eye Model ◽  
Goblet Cell Density

scholarly journals Dry eye disease among Glaucoma patients on topical hypotensive medications, in a tertiary hospital, Ethiopia

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Miraf Sahlu ◽  
Abeba T. Giorgis
Keyword(s):  
Dry Eye ◽  
Ocular Surface ◽  
Signs And Symptoms ◽  
Dry Eye Disease ◽  
Eye Disease ◽  
Eye Drops ◽  
Schirmer Test ◽  
Ocular Surface Disease Index ◽  
Cross Sectional ◽  
Corneal Staining

Abstract Background Dry eye disease is a multifactorial disease; causing various ocular symptoms with potential damage to the ocular surface. Applying hypotensive eye drops are presumed to initiate or exacerbate existing dry eye disease. The purpose of this study was to determine the frequency of signs and symptoms and severity of dry eye disease among glaucoma patients on topical hypotensive medications and controls. Methods A cross-sectional comparative study, involving 320 glaucoma patients and controls. Ocular Surface Disease Index (OSDI) symptoms score and Schirmer, tear breakup time and corneal staining tests were used to assess dry eye disease. Data was analyzed using SPSS version 24 software; p-value less than 0.05 was considered as statistically significant. Results Among the 160 study glaucoma patients, the mean duration of topical hypotensive medication use was 5.2 ± 5.21 years (range, 4 months - 32 years). Mild to severe level of OSDI score was found in 122 (76%) glaucoma patients and in 137 (86%) controls (p = 0.033). Mild to sever abnormal clinical tests in the glaucoma patients and control, respectively, were 106 (66%) vs 80 (50%) corneal staining (p = 0.045), 79 (49%) vs 72 (45%) TBUT (p = 0.021), and 91 (57%) vs 83 (52%) Schirmer test (p = 0.242). Test results at the level of sever: 2 (1%) vs 0 (0%) corneal staining, 50 (31%) vs 39 (24%) TBUT and 65 (41%) vs 60 (38%) Schirmer test in the glaucoma patents and controls, respectively. Corneal staining and TBUT had correlation with the number of drugs (p = 0.004 and 0.031, respectively), and more relationship of the two tests with total number of drops applied per day (p = 0.01 and p <  0.001, respectively). Patients on pilocarpine and timolol had more corneal staining and lower TBUT [(p = 0.011 and p <  0.001) and (p = 0.04 and 0.012), respectively]. Conclusions The study has identified glaucoma patients to be more affected by dry eye disease than non-glaucoma patients, and presence of significantly lower TBUT and higher corneal staining in the glaucoma patients on multidrops and multidose per day. We recommend consideration of evaluation and management of DED for glaucoma patients on multidrops and multidose hypotensive medications.

Impression cytology and meibomian glands in conjunctivochalasis

2021 ◽  
pp. 112067212110087
Author(s):  
Ersin Muhafiz ◽  
Erdinç Bozkurt ◽  
Remzi Erten
Keyword(s):  
Dropout Rate ◽  
Meibomian Gland ◽  
Control Group ◽  
Study Group ◽  
Impression Cytology ◽  
Schirmer Test ◽  
Significant Difference ◽  
Meibomian Glands ◽  
And Control ◽  
Margin Cytology

Purpose: To evaluate the tear parameters, meibomian glands and lid margin and tarsal conjunctival impression cytology in patients with conjunctivochalasis (CCH). Methods: The study included 57 patients diagnosed with CCH and 35 healthy volunteers. Tear break-up time (TBUT) was measured and Schirmer test was performed. Meibomian gland morphologies, dropout rates, and meiboscores were evaluated using meibography. Finally, impression cytology samples were taken by pressing the impression filters on the lower lid margin and lower tarsal conjunctiva. The samples were evaluated according to the Nelson grading system. Results: Schirmer test was longer and TBUT was shorter in the study group ( p = 0.035 and p < 0.001, respectively). The median of meibomian gland dropout rate was 33.45% (Interquartile range [IQR]; 23.17%–49.75%) and 18.80% (IQR; 12.35%–26.50%) in the study and control groups, respectively ( p < 0.001). There was no significant difference in terms of lid-margin cytology between the two groups ( p = 0.481). In tarsal conjunctiva cytology, the median value of Nelson grade of the study group was 2 (IQR; 1–2) and that of the control group was 1 (IQR; 1–2) ( p = 0.040). When Nelson grade-2 and above was accepted as the pathological limit, it was found that 27.5% of the study group and 15.2% of the control group showed pathological findings ( p = 0.204). Conclusions: In patients with CCH, damage occurs in the tarsal conjunctiva with the effects of redundant conjunctival folds. In these patients, atrophy occurs in the meibomian glands and tear stability is impaired. Therefore, CCH should not be overlooked in clinical practice.

scholarly journals Characterization of tear production in subjects with dry eye disease during intranasal tear neurostimulation: Results from two pivotal clinical trials

2019 ◽  
Vol 17 (1) ◽  
pp. 142-150 ◽  
Author(s):  
John D. Sheppard ◽  
Gail L. Torkildsen ◽  
Joel A. Geffin ◽  
Jung Dao ◽  
David G. Evans ◽  
...  
Keyword(s):  
Clinical Trials ◽  
Dry Eye ◽  
Dry Eye Disease ◽  
Eye Disease ◽  
Tear Production

scholarly journals Comparable effects on tear film parameters after femtosecond laser-assisted and conventional cataract surgery

2020 ◽  
Vol 40 (11) ◽  
pp. 3097-3104 ◽  
Author(s):  
Marc Schargus ◽  
Svetlana Ivanova ◽  
Gesa Stute ◽  
H. Burkhard Dick ◽  
Stephanie C. Joachim
Keyword(s):  
Femtosecond Laser ◽  
Cataract Surgery ◽  
Dry Eye ◽  
Surgical Techniques ◽  
Tear Film ◽  
Schirmer Test ◽  
Fluorescein Staining ◽  
Significant Difference ◽  
Eye Symptoms ◽  
Dry Eye Symptoms

Abstract Purpose Dry eye symptoms after conventional cataract surgery are a very common problem. Until now, only few data are available on objective tear film parameters in regard to femtosecond laser-assisted cataract surgery (LCS). Aim of this study was therefore to analyze and compare tear film parameter changes between LCS and conventional cataract surgery. Methods A consecutive group of 34 patients, scheduled for cataract surgery, were randomly selected for either LCS or conventional cataract surgery (17 patients/group). Tear film assessments including tear film osmolarity, Schirmer test, MMP-9 analysis via quantitative ELISA, corneal sensitivity, corneal fluorescein staining, and conjunctival fluorescein staining were sequentially evaluated pre- as well as 1 and 3 months postoperatively. Results Both groups showed no significant difference in baseline characteristics. All surgeries were performed without any complications. After 1 and 3 months, there was no statistically significant difference in regard to tear film osmolarity (1 month: p = 0.81, 3 months: p = 1.0), Schirmer test (1 month: p = 0.35, 3 month: p = 0.08), and MMP-9 concentration (1 month: p = 0.36, 3 month: p = 0.28) between the two groups. Conclusions Neither LCS nor conventional cataract surgery affected objective tear film parameters significantly during our 3-month postoperative observation period. Hence, both surgical techniques can be equally used to treat patients without prior dry eye symptoms.

A Modified Schirmer Test in Dry Eye and Normal Subjects: Open Versus Closed Eye and 1-Minute Versus 5-Minute Tests

Cornea ◽  
2010 ◽  
Vol 29 (4) ◽  
pp. 384-387 ◽  
Author(s):  
Mohsen Bahmani Kashkouli ◽  
Farzad Pakdel ◽  
Afsaneh Amani ◽  
Melika Asefi ◽  
Gholam Hosseyn Aghai ◽  
...  
Keyword(s):  
Dry Eye ◽  
Normal Subjects ◽  
Schirmer Test ◽  
Open Versus
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