scholarly journals Lifor Solution: An Alternative Preservation Solution in Small Bowel Transplantation

2016 ◽  
Vol 2016 ◽  
pp. 1-6
Author(s):  
Mingxiao Guo ◽  
Chunlei Lu ◽  
Ying Gao ◽  
Haifeng Zhang ◽  
Dongfeng Chen ◽  
...  
Keyword(s):  
Small Bowel ◽  
Intestinal Mucosa ◽  
Cold Storage ◽  
Small Bowel Transplantation ◽  
Preservation Solution ◽  
Uw Solution ◽  
Absorptive Function ◽  
Group 2 ◽  
Intestinal Absorptive Function ◽  
Group 1

Background and Objectives. The intestinal mucosa is extremely sensitive to ischemia. Better intestinal preservation is the first step to improve the results of intestinal transplantation. The aim of the study is to investigate the effect of cold Lifor solution on preservation of swine small bowel.Methods. Swine ileum segments (200 cm) were allotransplanted heterotopically after 9-hour cold storage with UW solution (group 1,n=6), with Lifor solution (group 2,n=6), or without storage (group 3,n=6), respectively. After cold storage, mucosal adenosine triphosphate (ATP) concentrations and histopathologic analysis after preservation were performed. At day 7 after the transplantation, intestinal absorptive function was also observed.Results. After 9 h cold preservation, pathological changes, the content of ATP in the intestinal mucosa, and the intestinal absorptive function after transplantation in group 2 were similar to those of group 1.Conclusion. The effect of cold storage of swine small bowel with Lifor solution is similar to that of UW solution. It may provide additional rationale for further exploration of Lifor as an alternative preservation solution in small bowel transplantation.

scholarly journals Ischemia-reperfusion histopathology alterations of the rabbit intestinal wall with and without exclusion of the collateral mesenteric circulation supply

2010 ◽  
Vol 25 (4) ◽  
pp. 318-321 ◽  
Author(s):  
Otoni Moreira Gomes ◽  
Geraldo Brasileiro Filho ◽  
Luiz Alberto Bomjardim Porto ◽  
Pedro Henrique de Lima Prata ◽  
Rafael de Mattos Paixão
Keyword(s):  
Small Bowel ◽  
Mesenteric Artery ◽  
Collateral Circulation ◽  
Ischemia Reperfusion ◽  
Celiac Artery ◽  
Artery Occlusion ◽  
Intestinal Wall ◽  
Group I ◽  
Group 2 ◽  
Group 1

PURPOSE: To evaluate the histopathology alterations of the intestinal mucosa of rabbits submitted to different times of mesenteric artery ischemia and reperfusion with and without celiac artery collateral circulation supply. METHODS: Two groups of eight male New Zealand white rabbits (weight 2.2-3.5 kg) were used in this study. In the Group 1 animals, the proximal mesenteric artery was occluded for 60 min with an atraumatic vascular clamp, followed by reperfusion for 60 min. In the Group 2 animals the small bowel and mesentery were cut 30cm and 60cm far from the gastroduodenal pyloric transition before the proximal mesenteric artery occlusion. Small bowel biopsies were obtained before ischemia (control), after 30 min and 60 min of mesenteric ischemia and at 30 and 60 min. of mesenteric artery reperfusion. RESULTS: In the Group I animals, the followings histopathology grade results were observed: t1, mean 0.4 + 0.29; t2, mean 1.9 ± 0.38; t3, 1.9 ± 0.33; t4, 1.2 ± 0.36 and t5, 1.2 ± 0.32. Differences between t0 and t2 and between t3 and t4 were statistically significant (p<0.05). Differences between t2 and t3 and t4 and t5 were not significant (p>0.5). In the Group II animals, it was observed: t1, mean 1.6 ± 0.33; t2, 2.4 ± 0.36; t3, 3.0 ± 0.35; t4 3.4 ± 0.31; t5, 3 ± 031. Differences between t0 and t1, t1 and t2, and t2 and t3 were significant (p<0.05). Differences between histopathology grades results of samples t1 to t5 in Group 1 and 2 were statistically significant (p<0.5). CONCLUSION: Microscopic examination of the biopsies revealed significant evidence of worse small bowel wall ischemia-reperfusion lesions by exclusion of the celiac artery collateral circulation supply.

Increase of interleukin-6 in the intestinal mucosa and muscularis following chronic rejection after small bowel transplantation

1998 ◽  
Vol 30 (6) ◽  
pp. 2600 ◽  
Author(s):  
K.J. Walgenbach ◽  
P.F. Heeckt ◽  
J.C. Kalff ◽  
J. Stanson ◽  
T.L. Whiteside ◽  
...  
Keyword(s):  
Small Bowel ◽  
Intestinal Mucosa ◽  
Interleukin 6 ◽  
Chronic Rejection ◽  
Small Bowel Transplantation

Refractory Anaemia Secondary to Small Bowel Angioectasias - Comparison between Endotherapy Alone versus Combination with Somatostatin Analogues

2017 ◽  
Vol 26 (4) ◽  
pp. 369-374 ◽  
Author(s):  
Stefania Chetcuti Zammit ◽  
Reena Sidhu ◽  
David Sanders
Keyword(s):  
Small Bowel ◽  
Argon Plasma Coagulation ◽  
Argon Plasma ◽  
Additional Treatment ◽  
Double Balloon Enteroscopy ◽  
Balloon Enteroscopy ◽  
Plasma Coagulation ◽  
Double Balloon ◽  
Group 2 ◽  
Group 1

Background & Aims: Patients with small bowel angioectasias (SBAs) can be difficult to manage as they are generally elderly with multiple co-morbidities. Angioectasias are multiple and tend to recur. Argon plasma coagulation (APC), despite being a commonly used method to treat these patients has an associated persistent rate of re-bleeding necessitating additional treatment to manage these patients.Methods: All patients with refractory iron deficiency anaemia secondary to SBAs were retrospectively subdivided into two groups. Patients in group 1 were managed with double balloon enteroscopy (DBE) and APC alone and those in group 2 received Lanreotide in addition to DBE and APC.Results: A total of 49 patients were included in this study: group 1: 37 patients (75.5%), group 2: 12 patients (24.5%). All had significant comorbidities and the mean duration of anaemia was 114.3, SD 307.0 months. Significant improvements in haemoglobin (Hb) (11g/L vs 3.2g/L p=0.043), transfusion requirements per month (0.8 vs 4.7 p=0.052) and mean bleeding episodes (1.08 vs 2.6 p=0.032) were demonstrated in group 2 when compared to group 1. One patient developed symptomatic gallstone disease and one patient stopped Lanreotide due to a lack of response.Conclusions: This is the first study comparing endotherapy to a combination of endotherapy and pharmacotherapy. It shows a significantly better outcome in patients receiving a combination of endotherapy and Lanreotide. Lanreotide can be a safe additional treatment in patients not responding to APC alone.Abbreviations: APC: argon plasma coagulation; CE: capsule endoscopy; DBE double balloon enteroscopy; Hb: hemoglobin; OGIB: over gastrointestinal bleeding; SBAs: small bowel angioectasias.

scholarly journals The Impact of Hydrated Aluminosilicates Supplemented in Litter and Feed on Chicken Growth, Muscle Traits and Gene Expression in the Intestinal Mucosa

Animals ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 2224
Author(s):  
Jakub Biesek ◽  
Aleksandra Dunisławska ◽  
Mirosław Banaszak ◽  
Maria Siwek ◽  
Marek Adamski
Keyword(s):  
Gene Expression ◽  
Body Weight ◽  
Intestinal Mucosa ◽  
Nutrient Sensing ◽  
Muscle Quality ◽  
Expression Of Genes ◽  
Group 3 ◽  
Group 2 ◽  
The Impact ◽  
Group 1

The aim of the study was to compare the production, muscle traits and gene expression in the intestinal mucosa of chickens supplemented with aluminosilicates in feed and litter simultaneously. A total of 300 Ross 308 were maintained for 42 days. Group 1 was the control group. In group 2, 0.650 kg/m2 of halloysite was added to the litter and 0.5–2% to the feed (halloysite and zeolite in a 1:1 ratio); in group 3, we added zeolite (0.650 kg/m2) to the litter and 0.5–2% to the feed. The production parameters, the slaughter yield and analyses of muscle quality were analyzed. There was a higher body weight, body weight gain and feed conversion ratio on day 18 and 33 in group 3, and a higher feed intake on day 19–33 in groups 2 and 3 than in 1. A lower water-holding capacity was found in the breasts of group 2 and in the legs of group 3 compared to group 1. The expression of genes related to the immune response, host defense and intestinal barrier and nutrient sensing in the intestinal tissue was analyzed. The results show a beneficial effect on the immune status of the host without an adverse effect on the expression of genes related to intestinal tightness or nutritional processes. Due to the growth, meat characteristics and the positive impact of immunostimulant and regulating properties, aluminosilicates can be suggested as a litter and feed additive in the rearing of chickens.

Long-term survival after multidisciplinary treatment of small-bowel neuroendocrine tumors with synchronous liver metastases: A single-institute experience.

2013 ◽  
Vol 31 (15_suppl) ◽  
pp. e15147-e15147
Author(s):  
Emilio Bertani ◽  
Nicola Fazio ◽  
Antonio Chiappa ◽  
Edoardo Botteri ◽  
Francesca Spada ◽  
...  
Keyword(s):  
Small Bowel ◽  
Liver Metastases ◽  
Neuroendocrine Tumors ◽  
Primary Tumor ◽  
Synchronous Liver Metastases ◽  
Surgery Group ◽  
Group 3 ◽  
Group 2 ◽  
The Impact ◽  
Group 1

e15147 Background: Neuroendocrine tumors (NETs) that originate in the distal jejunum and ileum are commonly diagnosed in stage IV, with liver metastases in 50%-75% of cases. The aim of this study was to ascertain the impact of surgery in a consecutive series of patients with small bowel NET and synchronous liver metastases treated at a single institute. Methods: Ninety-one patients managed between 1995 and 2011 were extracted from the institutional tumor registry. All patients had diagnosis by histology of small bowel NET and synchronous liver metastases. Results: Among the 91 patients 28 (30.8%) underwent primary tumor removal and liver metastases resection or intraoperative termal ablation (Group 1), 54 (59.3%) underwent resection of only the primary tumor (Group 2), and the remaining 9 (9.9%) did not receive any surgery (Group 3). After a median follow-up of 64 months an overall survival (OS) of 93.1%, 81.8% and 76.2% at 3, 5 and 8 years was registered for the whole group. Cancer-specific survival (CSS) was 93.1%, 83.3% and 77.6% at 3, 5 and 8 years, respectively. Five-years OS differed significantly according to the surgical approach (96.0% for Group 1 vs 79.8% for Group 2 vs 48.6% for Group 3, p=0.02). During the course of the illness octreotide was administered in 78 patients (85.7%), peptide receptor radiotherapy in 61 (67.0%), systemic chemotherapy in 21 (23.1%), hepatic artery embolization in 17 (18.7%), interferon in 11 (12.1%), molecular targeted agents in 2 (2.2%). For patients who underwent any surgery (Group 1 and Group 2) the multidisciplinary approach was associated with a better OS (85.7% vs 77.2% 5-years OS, p=0.04). In the multivariate analysis only age was associated with OS, HR: 1.05 (C. I.: 1.01-1.10). The results of CSS were comparable to those of OS. Conclusions: In this metastatic setting of small bowel NETs the 5 year OS was as high as 82% with a maximum benefit achievable for patients who had primary tumor resection and liver disease amenable to surgical or thermal ablation. However, the potential impact of age in prognosis should be carefully considered when choosing the treatment to be delivered in such patients.

scholarly journals Chimerism induction by nonmyeloablactive preconditioning and bone marrow infusion in rat small bowel transplantation

2003 ◽  
Vol 18 (1) ◽  
pp. 01-11
Author(s):  
Alexandre Bakonyi Neto ◽  
Mariana Behro ◽  
Phillip Ruiz ◽  
Evangelos P. Misiakos ◽  
Joshua Miller ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Small Bowel ◽  
Small Bowel Transplantation ◽  
Clinical Signs ◽  
Allograft Survival ◽  
Fk 506 ◽  
Short Course ◽  
Marrow Infusion ◽  
Bone Marrow Infusion ◽  
Group 1

In our previous work we demonstrated that the use of donor specific bone marrow infusions ( DSBMI ) after small bowel transplantation did not improve the graft survival after a short course of immunossupression. PURPOSE: In the current study, we evaluated whether recipient preconditioning with different regimens of radiation combined with DSBMI may enhance small bowel allograft survival with minimum recipient morbidity. METHODS: Heterotopic small bowel transplantation (SBTx) was performed with Lewis rats as recipients and DA rats as donors, which were immunossupressed with a short course of tacrolimus (FK 506 ) at 1mg/Kg/day for 5 days and distributed in 4 groups: group 1 (n= 4) without both irradiation and DSBMI; Groups 2 (n= 6), 3 (n= 9) and 4 (n= 6) received 100 x 10(6) DSBM cells at the time of the transplant. Groups 3 and 4 were irradiated with 250 and 400 rd respectively. Animals were examined daily for clinical signs of rejection or GVHD. Blood samples were taken weekly for chimeric studies by FC and intestinal biopsies were performed every 2 weeks. RESULTS: Animals in G1 and G2 had minimal rejection at day 15 after SBTx while GVHD was clinically and histologically characterized in G 3 and G 4. Total chimerism and T-cell chimerism was higher in irradiated groups when compared to non-irradiated groups. With exception of G1 and 2 where rejection was the cause of death, all animals in G3 and 4 died of GVHD. CONCLUSION:We concluded that low cytoreductive of irradiation can successfully decrease the graft rejection but not prevent the occurrence of GVHD.

scholarly journals Earlier use of capsule endoscopy in inpatients with melena or severe iron deficiency anemia reduces need for colonoscopy and shortens hospital stay

2018 ◽  
Vol 06 (09) ◽  
pp. E1075-E1084 ◽  
Author(s):  
Diana E. Yung ◽  
Anastasios Koulaouzidis ◽  
Sarah Douglas ◽  
John N. Plevris
Keyword(s):  
Gastrointestinal Tract ◽  
Small Bowel ◽  
Iron Deficiency ◽  
Gastrointestinal Endoscopy ◽  
Diagnostic Yield ◽  
Deficiency Anemia ◽  
Hospital Stays ◽  
Group 2 ◽  
Upper Gastrointestinal ◽  
Group 1

Abstract Background and study aims Capsule endoscopy(CE) is a well-established investigation for iron deficiency anemia (IDA) and melena, usually following negative upper and lower endoscopy. We aimed to study the effect of earlier CE in the investigative pathway for inpatients with IDA or melena at a large tertiary referral centre. Patients and methods We analyzed inpatients undergoing CE for IDA or melena from 2005 to 2017, without signs/symptoms suggesting lower gastrointestinal tract pathology. Patients underwent CE following negative upper and lower gastrointestinal endoscopy (Group 1), or negative upper gastrointestinal endoscopy (UGIE) only (Group 2). Results One hundred and seventy inpatients underwent CE for IDA (n = 44) and melena (n = 126). In Group 1, 46/95 (48.4%) patients had small bowel (SB) findings. CE found 16/95 (16.8 %) gastric and 12/95 (12.6 %) colon findings. Three of 12 patients with colon findings required repeat colonoscopy. One hundred and three colon investigations were carried out for 95 admissions. In Group 2, 33/75 (44.0%) patients had SB findings. There were 12/75 (16.0 %) gastric and 11/75 (14.7 %) colon findings. In patients with positive CE, significant colonic findings led to colonoscopy in 10 of 39 patients (diagnostic yield 6/10). Thirty-six patients had negative CE; 15 underwent colonoscopy (diagnostic yield 9/15). The remaining 21 of 36 patients with no further colonoscopy did not develop adverse outcomes related to colonic pathology. Twenty-six colon investigations were carried out in 75 admissions. Patients in Group 2 had shorter mean times from admission to CE (5.08 ± 3.80 vs. 6.38 ± 3.80 days; P = 0.02) and hospital stays (10.5 ± 9.58 vs. 12.5 ± 11.4 days; P = 0.04) compared to Group 1. Conclusion Earlier use of CE in inpatients with melena or IDA, no signs of lower gastrointestinal pathology and negative UGIE resulted in shortened hospital stays, significant DY from both small bowel and upper gastrointestinal tract, and two-thirds less unnecessary colon investigations without affecting clinical outcomes.

scholarly journals Release Profile of Salofalk 750 mg Tablets

1995 ◽  
Vol 9 (5) ◽  
pp. 247-250
Author(s):  
Vincent Ho ◽  
Joan E Blair ◽  
Zane Cohen ◽  
Robin S McLeod
Keyword(s):  
Small Bowel ◽  
Peak Plasma ◽  
Plasma Concentrations ◽  
Release Profile ◽  
Aminosalicylic Acid ◽  
Intestinal Transit Time ◽  
The Mean ◽  
Group 2 ◽  
Circuit Group ◽  
Group 1

This study determined the release profile of Salofalk 750 mg tablets (Axcan Pharma), an enteric-coated 5-aminosalicylic acid (5-ASA) preparation. Twenty-one ileostomates were divided into two groups and studied. Group 1 consisted of 10 subjects (five males, five females, mean age 39 years) who had a mean length of 65 cm of small bowel resected or out of circuit. Group 2 consisted of 11 subjects (eight males, three females, mean age 59 years) whose small bowel was intact. Following an overnight fast and collection of baseline samples, one Salofalk tablet was ingested. Ileostomy effluent and urine were collected for 24 h. Plasma samples were collected hourly for 6 h, then at 8, 12 and 24 h. All subjects ate standardized meals. All samples were stored at --10 degrees C and 5-ASA and N-ac-5-ASA (a metabolite of 5-ASA) were measured by high performance liquid chromatography. The mean intestinal transit time was not statistically different between the groups but the mean ileostomy effluent output was higher in group 1 versus group 2 (10.9 versus 13.1 h, P=0.4; 918 versus 606 mL, P=0.05). The mean peak plasma concentrations of 5-ASA and N-ac-5-ASA were not significantly different (6.12 and 5.42 µ g/ mL, P=0.8, respectively, in group 1 versus 6.75 and 6.66 µ g/mL, P=0.8 in group 2). On average, 33.1% of the ingested dose was recovered in the ileostomy effluent in group 1 versus 21.2% in group 2 (P=0.06) whereas the mean recovery in urine was 40.9% in group 1 but 62.9% in group 2 (P=0.001). These results suggest that 5-ASA is released in the small bowel. There was decreased absorption of 5-ASA and increased recovery of 5-ASA in the ileostomy effluent of subjects who had a small bowel resection.

scholarly journals Ischemia-reperfusion histopathology alterations of the rabbit intestinal wall with and without ischemic preconditioning

2011 ◽  
Vol 26 (4) ◽  
pp. 285-288 ◽  
Author(s):  
Otoni Moreira Gomes ◽  
Geraldo Brasileiro Filho ◽  
Rodrigo Lolli Almeida Salles ◽  
Rafaela Ervilha Linhares ◽  
Maria Cristina Nazar ◽  
...  
Keyword(s):  
Small Bowel ◽  
Ischemic Preconditioning ◽  
Mesenteric Artery ◽  
Mesenteric Ischemia ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Artery Occlusion ◽  
Group I ◽  
Group 2 ◽  
Group 1

PURPOSE: To evaluate the histopathology alterations of the intestinal mucosa of rabbits submitted to mesenteric artery ischemia and reperfusion with and without ischemic preconditioning. METHODS: Two groups of ten male New Zealand white rabbits body (weight 2.2-3.0, average 2.5 kg). For mesenteric ischemia induction in all animals the small bowel and mesentery were cut 30cm and 60cm far from the gastroduodenal pyloric transition before the proximal mesenteric artery occlusion. In the Group 1 animals, the proximal mesenteric artery was occluded for 45 min with an atraumatic vascular clamp, followed by reperfusion for 30 min. In the Group 2 the 45 min ischemic phase was preceded by three cycles of ischemia (2 minutes each) alternated with three cycles of reperfusion (2 minutes each). For istopathology study small bowel biopsies were obtained before ischemia (control), after 45 min of mesenteric ischemia and at 30 min. of mesenteric artery reperfusion. RESULTS: In the Group I animals, the followings histopathology grade results were observed: t1, mean 2,8; t2, mean 3,3. Using the Kruskal-Wallis non-parameter test, differences between t0 and t1 and t0 and t2 were significants (p<0.05), but not significant between t1 and t2 (p>0.05). In the Group 2 animals histopathology grade results were: t1 mean 2,6 and t2, mean 2,1. Differences between t0 and t1, t0 and t2 were significant (p<0.05). It was not observed differences (p>0.05) between results of t1 in both groups but histopathology injury observed in Group 1 t2 biopsies were higher (p<0.05) than observed in the same period (t2) of Group 2 animals. CONCLUSION: Microscopic examination of the biopsies revealed significant evidence of preconditioning protection against small bowel wall ischemia-reperfusion injury.

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