scholarly journals Ethylene-Induced Vinblastine Accumulation Is Related to Activated Expression of Downstream TIA Pathway Genes inCatharanthus roseus

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Xi Wang ◽  
Ya-Jie Pan ◽  
Bo-Wen Chang ◽  
Yan-Bo Hu ◽  
Xiao-Rui Guo ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Ethylene Response Factor ◽  
Response Factor ◽  
High Concentration ◽  
Metabolite Level ◽  
Peroxide Content ◽  
Qrt Pcr ◽  
Ethylene Response ◽  
Positive Effect ◽  
Loading Plot

We selected different concentrations of ethephon, to stressC. roseus. We used qRT-PCR and HPLC followed by PCA to obtain comprehensive profiling of the vinblastine biosynthesis in response to ethephon. Based on our findings, the results showed that the high concentration of ethephon had a positive effect at both transcriptional and metabolite level. Meanwhile, there was a remarkable decrease of hydrogen peroxide content and a promoted peroxidase activity in leaves. The loading plot combination with correlation analysis suggested thatCrPrx1could be regarded as a positive regulator and interacts with ethylene response factor (ERF) to play a key role in vinblastine content and peroxidase (POD) activity. This study provides the foundation for a better understanding of the regulation and accumulation of vinblastine in response to ethephon.

scholarly journals Expression Analysis of ERFs during Storage under Modified Atmosphere Packaging (High-concentration of CO2) of Fresh-cut Lotus Root

HortScience ◽  
2020 ◽  
Vol 55 (2) ◽  
pp. 216-223 ◽  
Author(s):  
En-chao Liu ◽  
Li-fang Niu ◽  
Yang Yi ◽  
Li-mei Wang ◽  
You-wei Ai ◽  
...  
Keyword(s):  
Phenylalanine Ammonia Lyase ◽  
Modified Atmosphere Packaging ◽  
Ethylene Response Factor ◽  
Modified Atmosphere ◽  
Response Factor ◽  
Biotic And Abiotic Stress ◽  
High Concentration ◽  
Ethylene Response ◽  
Lotus Root ◽  
Fresh Cut

Ethylene response factor (ERF) genes have been characterized in numerous plants, where they are associated with responses to biotic and abiotic stress. Modified atmosphere packaging (MAP) is an effective treatment to prevent lotus root browning. However, the possible relationship between ERF transcription factors and lotus root browning under MAP remains unexplored. In this study, the effects of phenol, phenylalanine ammonia lyase (PAL), polyphenol oxidase (PPO), and peroxidase (POD) enzyme activities; and PPO, PAL, POD, and ERF gene expression on fresh-cut lotus root browning were studied with MAP. The expression pattern of ERF2/5 correlated highly with the degree of browning. It is suggested that NnERF2/5 can be used as an important candidate gene for the regulation of fresh-cut lotus root browning under MAP, and the correlation of each gene should be studied further.

scholarly journals Role of ethylene and the APETALA 2/ethylene response factor superfamily in rice under various abiotic and biotic stress conditions

2017 ◽  
Vol 134 ◽  
pp. 33-44 ◽  
Author(s):  
Rambod Abiri ◽  
Noor Azmi Shaharuddin ◽  
Mahmood Maziah ◽  
Zetty Norhana Balia Yusof ◽  
Narges Atabaki ◽  
...  
Keyword(s):  
Biotic Stress ◽  
Stress Conditions ◽  
Ethylene Response Factor ◽  
Response Factor ◽  
Abiotic And Biotic Stress ◽  
Ethylene Response ◽  
Apetala 2

scholarly journals An AC-Rich Bean Element Serves as an Ethylene-Responsive Element in Arabidopsis

Plants ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 1033
Author(s):  
Chunying Wang ◽  
Tingting Lin ◽  
Mengqi Wang ◽  
Xiaoting Qi
Keyword(s):  
Nuclear Proteins ◽  
Ethylene Response Factor ◽  
Response Factor ◽  
Cis Acting ◽  
Gcc Box ◽  
Ethylene Response ◽  
Significant Difference ◽  
Responsive Element ◽  
Transcription Factor 1

Ethylene-responsive elements (EREs), such as the GCC box, are critical for ethylene-regulated transcription in plants. Our previous work identified a 19-bp AC-rich element (ACE) in the promoter of bean (Phaseolus vulgaris) metal response element-binding transcription factor 1 (PvMTF-1). Ethylene response factor 15 (PvERF15) directly binds ACE to enhance PvMTF-1 expression. As a novel ERF-binding element, ACE exhibits a significant difference from the GCC box. Here, we demonstrated that ACE serves as an ERE in Arabidopsis. It conferred the minimal promoter to respond to the ethylene stress and inhibition of ethylene. Moreover, the cis-acting element ACE could specifically bind the nuclear proteins in vitro. We further revealed that the first 9-bp sequence of ACE (ACEcore) is importantly required by the binding of nuclear proteins. In addition, PvERF15 and PvMTF-1 were strongly induced by ethylene in bean seedlings. Since PvERF15 activates PvMTF-1 via ACE, ACE is involved in ethylene-induced PvMTF-1 expression. Taken together, our findings provide genetic and biochemical evidence for a new ERE.

scholarly journals UV-C radiation modifies the ripening and accumulation of ethylene response factor (ERF) transcripts in tomato fruit

2015 ◽  
Vol 102 ◽  
pp. 9-16 ◽  
Author(s):  
Joseana Severo ◽  
Aline Tiecher ◽  
Jullien Pirrello ◽  
Farid Regad ◽  
Alain Latché ◽  
...  
Keyword(s):  
Tomato Fruit ◽  
Ethylene Response Factor ◽  
Response Factor ◽  
Ethylene Response ◽  
Uv C
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