scholarly journals Fabrication of Novel Hydrogel with Berberine-Enriched Carboxymethylcellulose and Hyaluronic Acid as an Anti-Inflammatory Barrier Membrane

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Yu-Chih Huang ◽  
Kuen-Yu Huang ◽  
Bing-Yuan Yang ◽  
Chun-Han Ko ◽  
Haw-Ming Huang
Keyword(s):  
Hyaluronic Acid ◽  
Testing Machine ◽  
Clinical Applications ◽  
Postsurgical Complications ◽  
Barrier Membrane ◽  
Universal Testing ◽  
Griess Reaction ◽  
Hydrogel Membrane ◽  
Anti Inflammatory

An antiadhesion barrier membrane is an important biomaterial for protecting tissue from postsurgical complications. However, there is room to improve these membranes. Recently, carboxymethylcellulose (CMC) incorporated with hyaluronic acid (HA) as an antiadhesion barrier membrane and drug delivery system has been reported to provide excellent tissue regeneration and biocompatibility. The aim of this study was to fabricate a novel hydrogel membrane composed of berberine-enriched CMC prepared from bark of theP. amurensetree and HA (PE-CMC/HA). In vitro anti-inflammatory properties were evaluated to determine possible clinical applications. The PE-CMC/HA membranes were fabricated by mixing PE-CMC and HA as a base with the addition of polyvinyl alcohol to form a film. Tensile strength and ultramorphology of the membrane were evaluated using a universal testing machine and scanning electron microscope, respectively. Berberine content of the membrane was confirmed using a UV-Vis spectrophotometer at a wavelength of 260 nm. Anti-inflammatory property of the membrane was measured using a Griess reaction assay. Our results showed that fabricated PE-CMC/HA releases berberine at a concentration of 660 μg/ml while optimal plasticity was obtained at a 30 : 70 PE-CMC/HA ratio. The berberine-enriched PE-CMC/HA had an inhibited 60% of inflammation stimulated by LPS. These results suggest that the PE-CMC/HA membrane fabricated in this study is a useful anti-inflammatory berberine release system.

scholarly journals Shear Bond Strength of Different Adhesive Materials used for Bonding Orthodontic Brackets : A Comparative in vitro Study

2015 ◽  
Vol 5 (1) ◽  
pp. 22-26
Author(s):  
Muzin Shahi Shaik ◽  
Snigdha Pattanaik ◽  
Sudhakar Pathuri ◽  
Arunachalam Sivakumar
Keyword(s):  
Bond Strength ◽  
Treatment Duration ◽  
Shear Bond Strength ◽  
Testing Machine ◽  
Test Group ◽  
In Vitro Study ◽  
Universal Testing ◽  
Materials Used ◽  
Adhesive Materials

Introduction: Bond strength is an important property and determines the amount of force delivered and treatment duration in orthodontics. Many light-cured bonding materials are being used; but it is required to determine the most efficient one withdesired bond strength. Objective: To determine and compare the shear bond strength of three visible light-cured composites (Transbond XT, Heliositand Enlight) and two self-cured composites (Rely-a-bond and Concise). Materials & Method: 100 extracted premolars were collected and randomly divided into 5 test groups of different adhesives. Brackets were bonded to the teeth in each test group with the respective adhesive according to the manufacturer’s instructions. Each specimen was debonded using Universal Testing Machine and the shear bond strength for each specimen was calculated. All the groups were compared by ANOVA one-way test. Results: There were statistically significant differences among the five groups (P<0.05). The shear bond strength of Enlight (13.92 ± 3.92) is similar to Transbond XT (14.30 ± 4.35). Conclusion: Light cure composites showed higher bond strength than self cure composites.

scholarly journals Evaluation of friction produced by self-ligating, conventional and Barbosa Versatile brackets

2016 ◽  
Vol 45 (2) ◽  
pp. 71-77 ◽  
Author(s):  
Jurandir Antonio BARBOSA ◽  
Carlos Nelson ELIAS ◽  
Roberta Tarkany BASTING
Keyword(s):  
Testing Machine ◽  
Frictional Resistance ◽  
The Self ◽  
Factorial Anova ◽  
Steel Wires ◽  
Universal Testing ◽  
Conventional Brackets ◽  
Conventional Type ◽  
Bracket Design

Abstract Introduction The Barbosa Versatile bracket design may provide lower frictional force and greater sliding. However, no in vitro studies have shown its sliding mechanisms and frictional resistance, particularly in comparison with other self-ligating or conventional brackets. Objective To compare the frictional resistance among self-ligating brackets (EasyClip/ Aditek, Damon MX/ Ormco and In Ovation R/ GAC); conventional brackets (Balance Roth/ GAC, and Roth Monobloc/ Morelli); and Barbosa Versatile bracket (Barbosa Versatile/ GAC) with different angles and arch wires. Material and method Brackets were tested with the 0.014", 0.018", 0.019"×0.025" and 0.021"×0.025" stainless steel wires, with 0, 5, 10, 15 and 20 degree angulations. Tying was performed with elastomeric ligature for conventional and Barbosa Versatile brackets, or with a built-in clip system of the self-ligating brackets. A universal testing machine was used to obtain sliding strength and friction value readouts between brackets and wires. Result Three-way factorial ANOVA 4×5×6 (brackets × angulation × wire) and Tukey tests showed statistically significant differences for all factors and all interactions (p<0.0001). Static frictional resistance showed a lower rate for Barbosa Versatile bracket and higher rates for Roth Monobloc and Balance brackets. Conclusion The lowest frictional resistance was obtained with the Barbosa Versatile bracket and self-ligating brackets in comparison with the conventional type. Increasing the diameter of the wires increased the frictional resistance. Smaller angles produced less frictional resistance.

scholarly journals Evaluation of Different in Vitro Testing Methods for Mechanical Properties of Veneer Ceramics/ Евалуација На Тестовите In Vitro За Одредување На Механичките Особини На Порцеланските Маси За Фасетирање

PRILOZI ◽  
2015 ◽  
Vol 36 (1) ◽  
pp. 225-230 ◽  
Author(s):  
Aneta Mijoska ◽  
Mirjana Popovska
Keyword(s):  
Flexural Strength ◽  
Mechanical Characteristics ◽  
Testing Machine ◽  
In Vitro Testing ◽  
Biaxial Test ◽  
Testing Methods ◽  
Dental Ceramic ◽  
Universal Testing ◽  
Biaxial Tests

Abstract Metal-ceramic and all-ceramic prosthetic restorations in the patient mouth are often damaged by esthetic and functional problems that reduce their success and longevity. Аim: To evaluate methods for testing mechanical characteristics of dental ceramics through analysis of different testing methods. Material and methods: Dental ceramic materials are tested with in vivo and in vitro methods for their most important mechanical characteristics: hardness, toughness, flexural strength and abrasion. In vitro testing methods are faster and more efficient, without subjective factors from the patient according to ISO standards. Testing is done with universal testing machines, like Zwick 1445, Universal Testing Machine (Zwick DmbH & Co.KG, Ulm, Germany), Instron 4302 (Instron Corporation, England), MTS Sintech ReNew 1123 or in oral chewing simulators. Results: According to the testing results, flexure strength is one of the most important characteristic of the dental ceramic to be tested, by the uniaxial and biaxial tests. Uniaxial tests three-point and four-point flexure are not most appropriate because the main stress on the lower side of the tested specimens is tension that causes beginning fractures at the places with superficial flow. Uniaxial results for flexural strength are lower than actual force, while with biaxial test defects and flows on the edges of tested specimens are not directly loaded. Conclusion: Biaxial flexural method has advantages over uniaxial because of real strength results, but also for simple shape and preparing of the testing specimens.

scholarly journals Effect of Chemical Disinfectant on the Transverse Strength of Heat-polymerized Acrylic Resins Subjected to Mechanical and Chemical Polishing: An in vitro Study

2012 ◽  
Vol 13 (3) ◽  
pp. 389-393
Author(s):  
Deepthi Kalahasti ◽  
Smitha Sharan ◽  
Harish Konde
Keyword(s):  
Testing Machine ◽  
In Vitro Study ◽  
Chemical Polishing ◽  
Denture Base ◽  
Transverse Strength ◽  
Acrylic Resins ◽  
Universal Testing ◽  
Chemical Solutions ◽  
Chemical Disinfectant

ABSTRACT Aims To evaluate the effect of chemical disinfectant on the transverse strength of heat-polymerized acrylic resins subjected to mechanical and chemical polishing. Materials and methods A total of 256 rectangular specimens (65 × 10 × 3 mm) 128 per resin (Lucitone-199 and Acralyn-H) were fabricated. One side of each specimen was not polished and the other was either mechanically (n = 96) or chemically (n = 96) polished and immersed for 10, 30 and 60 minutes in 2% alkaline glutaraldehyde. Mechanically polished (n = 32) and chemically polished (n = 32) control specimens were immersed only in distilled water. The transverse strength (N/mm2) was tested for failure in a universal testing machine, at a crosshead speed of 5 mm/min. Data were statistically analyzed using 2-way ANOVA and Student t-test. Results chemical polishing resulted in significantly lower transverse strength values than mechanical polishing. Lucitone- 199 resin demonstrated the highest overall transverse strength for the materials tested. Heat-polymerized acrylic resins either mechanically or chemically polished, did not demonstrate significant changes in transverse strength during immersion in the disinfecting solution tested, regardless of time of immersion. Conclusion Lucitone-199 resin demonstrated the highest overall transverse strength for the materials tested and significantly stronger than Acralyn-H with either type of polishing following immersion in 2% alkaline glutaraldehyde. Clinical significance There is a concern that immersion in chemical solutions often used for cleansing and disinfection of prostheses may undermine the strength and structure of denture base resins. In this study it was observed that, the transverse strength of samples of Lucitone-199 was higher than that of the samples of Acralyn-H. The chances of fracture of the denture made of Lucitone-199 are less than that of dentures made of Acralyn-H. The chemically polished dentures may be more prone to fracture than mechanically polished dentures. How to cite this article Sharan S, Kavitha HR, Konde H, Kalahasti D. Effect of Chemical Disinfectant on the Transverse Strength of Heat-polymerized Acrylic Resins Subjected to Mechanical and Chemical Polishing: An in vitro Study. J Contemp Dent Pract 2012;13(3):389-393.

Preparation and characterization of antibacterial and anti-inflammatory hyaluronic acid-chitosan-dexamethasone hydrogels for peri-implantitis repair

2021 ◽  
pp. 088532822110479
Author(s):  
Zhen Zhou ◽  
Qiang Zhang ◽  
Yamin Wang
Keyword(s):  
Hyaluronic Acid ◽  
Physicochemical Characterization ◽  
Inflammatory Drug ◽  
Composite Hydrogels ◽  
Tnf Α ◽  
Anti Inflammatory ◽  
Nih 3T3

Numerous treatment methods for peri-implantitis have been widely used including oral cleaning, traditional metal scraping means, or local antibiotic application. However, to continuously release antibacterial and anti-inflammatory drug in location in situ for effective peri-implantitis repair is still challenging. Herein, an anti-inflammatory drug dexamethasone (DE)–incorporated hyaluronic acid (HA)-chitosan (CT) composite hydrogels system was developed to repair peri-implantitis. The physicochemical characterization and biocompatibility of the hydrogel were evaluated in vitro. The in vivo hydrogels degradation and peri-implantitis repair were assessed in mice. The results showed that the prepared multifunctional hydrogels achieved sustained release, with an equilibrium swelling of 18, and promoted the growth against NIH-3T3 fibroblast cells. The in vitro antibacterial tests showed HA-CT-DE hydrogels can inhibit methicillin-resistant Staphylococcus aureus and Escherichia coli. It down-regulated the expression levels of inflammation factor IL-1β, IL-6 and, TNF-α in peri-implantitis. The prepared HA-CT-DE composite hydrogels with integrated function is promising for the treatment of peri-implantitis.

Fracture Resistance of Implant Abutments Following Abutment Alterations by Milling the Margins: An In Vitro Study

2016 ◽  
Vol 42 (6) ◽  
pp. 464-468 ◽  
Author(s):  
Anuya Patankar ◽  
Mohit Kheur ◽  
Supriya Kheur ◽  
Tabrez Lakha ◽  
Murtuza Burhanpurwala
Keyword(s):  
Fracture Resistance ◽  
Testing Machine ◽  
In Vitro Study ◽  
Vitro Study ◽  
Maximum Resistance ◽  
Universal Testing ◽  
Implant Abutments ◽  
Implant Abutment ◽  
Different Levels

This in vitro study evaluated the effect of different levels of preparation of an implant abutment on its fracture resistance. The study evaluated abutments that incorporated a platform switch (Myriad Plus Abutments, Morse Taper Connection) and Standard abutments (BioHorizons Standard Abutment, BioHorizons Inc). Each abutment was connected to an appropriate implant and mounted in a self-cured resin base. Based on the abutment preparation depths, 3 groups were created for each abutment type: as manufactured, abutment prepared 1 mm apical to the original margin, and abutment prepared 1.5 mm to the original margin. All the abutments were prepared in a standardized manner to incorporate a 0.5 mm chamfer margin uniformly. All the abutments were torqued to 30 Ncm on their respective implants. They were then subjected to loading until failure in a universal testing machine. Abutments with no preparation showed the maximum resistance to fracture for both groups. As the preparation depth increased, the fracture resistance decreased. The fracture resistance of implant abutment junction decreases as the preparation depth increases.

scholarly journals Evaluation of the Dislodgement Resistance of a New Pozzolan-Based Cement (EndoSeal MTA) Compared to ProRoot MTA and Biodentine in the Presence and Absence of Blood

Scanning ◽  
2019 ◽  
Vol 2019 ◽  
pp. 1-6 ◽  
Author(s):  
Alireza Adl ◽  
Nooshin Sadat Shojaee ◽  
Negar Pourhatami
Keyword(s):  
Bond Strength ◽  
Testing Machine ◽  
Time Intervals ◽  
Failure Patterns ◽  
Tukey Test ◽  
Universal Testing ◽  
Presence And Absence ◽  
Type Of Failure ◽  
Push Out

Introduction. This in vitro study investigated the dislodgement resistance of EndoSeal MTA, a new pozzolan-containing calcium silicate-based material, in comparison with ProRoot MTA and Biodentine in the presence and absence of contamination with blood. Methods. Standard furcal perforations were created in 180 human mandibular first molars. The teeth were randomly allocated to 12 groups of 15 each. ProRoot MTA, Biodentine, and EndoSeal MTA were used to repair the perforations. In half of the samples, the walls of the perforated areas were contaminated with blood, whereas saline was injected into the other half. A push-out test was performed using a universal testing machine after 24 hours or 7 days. To evaluate failure patterns, the samples were split into half and were examined under a stereomicroscope at a 20x magnification. Data were analyzed using three-way analysis of variance, Tukey test, and Student’s t-test. Results. At both time intervals and in the presence and absence of contamination with blood, ProRoot MTA and Biodentine had significantly higher retention values than EndoSeal MTA (p<0.001). Contamination with blood had no effect on EndoSeal MTA; however, it negatively affected the dislodgement resistance of Biodentine at 24 hours and ProRoot MTA at both time intervals (p<0.05). Time significantly affected only the bond strength of the uncontaminated groups (p>0.001). The most common type of failure was mixed for ProRoot MTA and Biodentine, whereas it was cohesive for EndoSeal MTA. Conclusions. ProRoot MTA and Biodentine showed higher values of bond strength than EndoSeal MTA and may thus be better options for the repair of root perforations.

scholarly journals The Viability and Anti-Inflammatory Effects of Hyaluronic Acid-Chitlac-Tracimolone Acetonide-β-Cyclodextrin Complex on Human Chondrocytes

Cartilage ◽  
2020 ◽  
pp. 194760352090865 ◽  
Author(s):  
Elena Tarricone ◽  
Rossella Elia ◽  
Elena Mattiuzzo ◽  
Alessia Faggian ◽  
Assunta Pozzuoli ◽  
...  
Keyword(s):  
Gene Expression ◽  
Hyaluronic Acid ◽  
Articular Chondrocytes ◽  
Quantitative Polymerase Chain Reaction ◽  
Polymerase Chain Reaction Analysis ◽  
Human Chondrocytes ◽  
Cyclodextrin Complex ◽  
Modified Chitosan ◽  
Anti Inflammatory

Objective To compare the effects of the complex triamcinolone acetonide-hydroxypropyl-β-cyclodextrin (TA-CD) on in vitro inflamed primary human articular chondrocytes in the presence or absence of the mixture hyaluronic acid-Chitlac, a lactose-modified chitosan (HA-CTL). Design Changes in cell viability and pro-inflammatory cytokines gene expression were analyzed in human chondrocytes using an in vitro model of macrophage-mediated inflammation. Human monocytes U937 were differentiated to macrophages by phorbol 12-myristate 13-acetate (PMA) and lipopolysaccharides (LPS). The anti-inflammatory effects of the complex TA-CD and HA-CTL mixture were assessed on chondrocytes exposed for 24 hours to U937 conditioned medium (CM), by quantitative polymerase chain reaction analysis. Results The TA-CD viability was enhanced by the presence of the HA-CTL mixture in chondrocyte cultures. The exposure of cells to CM significantly increased interleukin-1β and interleukin-6 gene expression, and when the complex TA-CD was added to the inflamed cells, gene transcription of cytokines was restored to near baseline values, both in the presence or in the absence of HA-CTL mixture. Conclusion The addition of HA-CTL mixture significantly attenuated cytotoxicity induced by TA and preserved the anti-inflammatory effects, thus confirming the chondroprotective role of the HA-CTL mixture.

A Novel Approach to Prove Bacterial Leakage of Implant-Abutment Connections In Vitro

2016 ◽  
Vol 42 (6) ◽  
pp. 452-457 ◽  
Author(s):  
Andreas Wachtel ◽  
Tycho Zimmermann ◽  
Tobias Spintig ◽  
Florian Beuer ◽  
Wolf-Dieter Müller ◽  
...  
Keyword(s):  
Color Change ◽  
Testing Machine ◽  
Loading Test ◽  
Universal Testing ◽  
Novel Approach ◽  
Implant Abutment ◽  
Optical Indicator ◽  
Bacterial Leakage ◽  
Iso 14801

Bacterial leakage from the implant-abutment-interface (IAI) is suspected of contributing to the development of peri-implantitis. The aim of the study was to develop a straightforward test setup to evaluate the bacterial leakage of the IAI of 2-piece implant systems under laboratory conditions. A test suspension of Enterococcus faecium was injected into 7 implants (PerioType Rapid Implants) prior to abutment fixation. The IAI was covered by kanamycin aesculn azide agar (KAAA), which serves as an optical indicator for E. faecium. The specimens were cyclically loaded with a force of 120 N for up to 1 000 000 cycles in a universal testing machine in accordance with the ISO 14801:2007 standard. The color change of the KAAA was recorded. Three of the 7 implants showed bacterial leakage before the cyclic loading test started. The bacterial tightness of the IAIs of the 4 remaining implants lasted for 35 680 ± 22 467 cycles on average. The experimental setup at hand provides the means for a straightforward evaluation of the bacterial tightness of the IAI of 2-piece dental implants.

scholarly journals In vitro force delivery of nickel-titanium superelastic archwires in vertical displacement

2012 ◽  
Vol 17 (6) ◽  
pp. 26-30
Author(s):  
Aisha de Souza Gomes Stumpf ◽  
Karina dos Santos Mundstock ◽  
Daniel Mundstock ◽  
Carlos Alberto Mundstock
Keyword(s):  
Tooth Movement ◽  
Testing Machine ◽  
Rocky Mountain ◽  
Vertical Displacement ◽  
Nickel Titanium ◽  
Stress Release ◽  
Maximum Displacement ◽  
Universal Testing ◽  
Temperature Controlled

OBJECTIVE: The purpose of this study was to evaluate the force delivered by different superlastic nickel-titanium wires during vertical displacement, in order to determine whether their stress release meets the criteria for constant and light forces that are usually accredited to these archwires. METHOD: Ten samples of 6 brands of 0.016-in archwires (Ormco, GAC, Morelli, TP, American Orthodontics e Rocky Mountain) were tested in a complete metal model using Dynalock brackets (3M Unitek™). In the canine position, there was a sliding bracket connected to a pole. This set was related to a load cell of 0.5 kg attached to a universal testing machine (Autograph AG-199kNG, Shimadzu). The crosshead speed was 0.5 mm/min and the maximum displacement was 1.0 mm. The model was submerged in temperature-controlled water. The results were analyzed by ANOVA (p < 0.05), using the software SAS System 8.02, Cry, NC, USA. RESULTS: The TP archwire had the lowest force throughout the test, although the final force was high (277.91 g). The Rocky Mountain archwire had the highest force release (455.41 g). CONCLUSION: The different brands of wires tested in this study failed in delivering low and constant forces as expected from superlastic nickel-titanium wires. The forces were extremely heavy for a vertical tooth movement.

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