scholarly journals Mitigating the Risk of Transfusion-Transmitted Dengue in Australia

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Kelly Rooks ◽  
Clive R. Seed ◽  
Jesse J. Fryk ◽  
Catherine A. Hyland ◽  
Robert J. Harley ◽  
...  
Keyword(s):  
Blood Donors ◽  
Nonstructural Protein ◽  
Dengue Infection ◽  
Screening Assay ◽  
No Donors ◽  
Nonstructural Protein 1 ◽  
Dengue Transmission ◽  
Blood Donor Screening ◽  
Dengue Outbreaks ◽  
Risk Areas

Dengue viruses (DENV 1–4) are a risk to transfusion safety, with several transfusion-transmitted (TT) cases reported globally. DENV 1–4 are endemic in over 100 countries, with seasonal outbreaks occurring in northeastern Australia. To mitigate TT-DENV risk in Australia, fresh blood components are not manufactured from donors returning from any area (domestic/overseas) with known dengue transmission. Alternatively, TT-DENV risk may be mitigated using an appropriate blood donor screening assay. We aimed to determine the rate of dengue infection in donors during dengue outbreaks in Australia. Plasma samples were collected from blood donors during local dengue outbreaks. All samples were tested for the presence of DENV RNA and selected samples were tested for DENV antigen (nonstructural protein 1, NS1) with two assays. No donors residing in high risk areas had detectable levels of DENV RNA or NS1 and no cases of DENV viremia were detected in blood donors residing in areas of Australia experiencing DENV outbreaks. Definitive conclusions could not be drawn from this study; however, the lack of detection of DENV RNA or antigen in donations suggests that the current risk of TT-DENV is low and maintaining the fresh component restriction for “at-risk” donors is appropriate.

scholarly journals Performance of a New Microfluidic Dengue NS1 Immuno-magnetic Agglutination Assay for the Rapid Diagnosis of Dengue Infection in Adults

2021 ◽  
Author(s):  
Ekkarat Wongsawat ◽  
Yupin Suputtamongkol ◽  
Susan Assanasaen ◽  
Saowaluk Silpasakorn ◽  
Panisadee Avirutnan ◽  
...  
Keyword(s):  
Detection Rate ◽  
Nonstructural Protein ◽  
Dengue Infection ◽  
Main Tool ◽  
Rapid Diagnosis ◽  
Detection Methods ◽  
Health Concern ◽  
Diagnostic Purpose ◽  
Agglutination Assay ◽  
Nonstructural Protein 1

Dengue (DENV) infections are a public health concern worldwide and thus early diagnosis is important to ensure appropriate clinical management. The rapid diagnostic test (RDT) targets nonstructural protein 1 (NS1) detection and is the main tool used for diagnostic purpose. In this study, we evaluated the performance of a new rapid and semi-quantitative microfluidic DENV NS1 immuno-magnetic agglutination assay or IMA (ViroTrack Dengue Acute, BluSense Diagnostics, Copenhagen, Denmark). We studied 233 subjects confirmed to have DENV infection (by a real-time reverse transcriptase polymerase chain reaction) and 200 control samples were taken from patients with confirmed diagnoses of other febrile illnesses, in Thailand. Samples were tested using the NS1 antigen (Ag) detection methods: in-house NS1 Ag ELISA (ELISA), SD BIOLINE Dengue NS1 Ag RDT (ICT), and ViroTrack Dengue Acute (IMA). Sensitivities of these tests were 86.3%, 78.9%, and 85.5%, respectively. All tests showed high specificity (100%, 99%, and 97% for ELISA, ICT, and IMA, respectively). The sensitivities of both RDTs were affected by the low sensitivity to DENV-2 and DENV-4. NS1 Ag was detected in every patient on day 1 and day 2 after onset of illness by ELISA and IMA with a decline in detection rates over time after day 6 of illness. NS1 detection rate using ICT decreased from 100% on day 1 of illness to 98.6% on day 2 after onset of illness. By day 6, the detection rate was 45.9%. Thus, IMA performed better than ICT for early and rapid diagnosis of DENV infections in endemic countries.

scholarly journals Quantitative NS1 antigen and the severity of dengue virus infections

2015 ◽  
Vol 55 (2) ◽  
pp. 87
Author(s):  
Ni Made Adi Purnami ◽  
Mohammad Juffrie ◽  
Made Gde Dwi Lingga Utama
Keyword(s):  
Clinical Course ◽  
Nonstructural Protein ◽  
Dengue Infection ◽  
Hemorrhagic Fever ◽  
Cross Sectional Study ◽  
Severe Dengue ◽  
Virus Infections ◽  
Cross Sectional ◽  
Nonstructural Protein 1 ◽  
Spearman Test

Background Dengue infection is one of the main cause ofmorbidity and mortality in children in Indonesia. Since it is knownthat earlier treatment and supportive therapies can decreased casefatality rate from dengue hemorrhagic fever (DHF), identificationof children who have risks to develop to DHF must be quicklyidentified, mainly in areas of endemic.Objective To find a correlation between increased quantitativesecreted nonstructural protein-1 (sNS1) with clinical course ofsevere dengue infections.Methods This was a cross-sectional study conducted on childrenwith dengue infections in Tropical Infections Division of ChildHealth Department, Sanglah Hospital, Denpasar. Detection ofthe dengue antigen was made by examining sNS1 quantitativeimmuno-assay. Analysis correlation of Spearman test was used tolook the relationship between increased quantitative sNS1 withclinical course of severe dengue infections.Results There was a positive relationship between quantitativesNS1 and clinical course of severe dengue infections with a valueof r = 0.903, P=0.001. Increased sNS1 level had a positivecorrelation with more severe dengue infections.Conclusions Quantitative sNS1 titer has a strong positivecorrelation with clinical course of severe dengue infections.

scholarly journals Dengue nonstructural protein 1 antigen in the urine as a rapid and convenient diagnostic test during the febrile stage in patients with dengue infection

2011 ◽  
Vol 71 (4) ◽  
pp. 467-469 ◽  
Author(s):  
Ampaiwan Chuansumrit ◽  
Wathanee Chaiyaratana ◽  
Kanchana Tangnararatchakit ◽  
Sutee Yoksan ◽  
Marie Flamand ◽  
...  
Keyword(s):  
Diagnostic Test ◽  
Nonstructural Protein ◽  
Dengue Infection ◽  
Nonstructural Protein 1

scholarly journals Use of a Rapid Test for Diagnosis of Dengue during Suspected Dengue Outbreaks in Resource-Limited Regions

2016 ◽  
Vol 54 (8) ◽  
pp. 2090-2095 ◽  
Author(s):  
Elizabeth A. Hunsperger ◽  
Tyler M. Sharp ◽  
Paul Lalita ◽  
Kini Tikomaidraubuta ◽  
Yolanda Rebello Cardoso ◽  
...  
Keyword(s):  
Public Health ◽  
Sensitivity And Specificity ◽  
Nonstructural Protein ◽  
Clinical Care ◽  
Rapid Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Major Public Health Problem ◽  
Public Health Response ◽  
Nonstructural Protein 1 ◽  
Dengue Outbreaks

Dengue is major public health problem, globally. Timely verification of suspected dengue outbreaks allows for public health response, leading to the initiation of appropriate clinical care. Because the clinical presentation of dengue is nonspecific, dengue diagnosis would benefit from a sensitive rapid diagnostic test (RDT). We evaluated the diagnostic performance of an RDT that detects dengue virus (DENV) nonstructural protein 1 (NS1) and anti-DENV IgM during suspected acute febrile illness (AFI) outbreaks in four countries. Real-time reverse transcription-PCR and anti-DENV IgM enzyme-linked immunosorbent assay were used to verify RDT results. Anti-DENV IgM RDT sensitivity and specificity ranged from 55.3 to 91.7% and 85.3 to 98.5%, respectively, and NS1 sensitivity and specificity ranged from 49.7 to 92.9% and 22.2 to 89.0%, respectively. Sensitivity varied by timing of specimen collection and DENV serotype. Combined test results moderately improved the sensitivity. The use of RDTs identified dengue as the cause of AFI outbreaks where reference diagnostic testing was limited or unavailable.

Evaluation of dengue nonstructural protein 1 antigen strip for the rapid diagnosis of patients with dengue infection

2009 ◽  
Vol 64 (1) ◽  
pp. 83-84 ◽  
Author(s):  
Wathanee Chaiyaratana ◽  
Ampaiwan Chuansumrit ◽  
Viroj Pongthanapisith ◽  
Kanchana Tangnararatchakit ◽  
Sarapee Lertwongrath ◽  
...  
Keyword(s):  
Nonstructural Protein ◽  
Dengue Infection ◽  
Rapid Diagnosis ◽  
Nonstructural Protein 1

Dengue outbreak 2018 in district Shangla KPK; clinical features and laboratory markers of dengue virus infection

2020 ◽  
Vol 15 (10) ◽  
pp. 693-699
Author(s):  
Faheem Anwar ◽  
Muhammad Tayyab ◽  
Muhammad Salman ◽  
Abdullah ◽  
Misbahud Din ◽  
...  
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Nonstructural Protein ◽  
Immunoglobulin M ◽  
Dengue Virus Infection ◽  
Female Ratio ◽  
Dengue Outbreak ◽  
Nonstructural Protein 1 ◽  
Laboratory Markers ◽  
Dengue Outbreaks

Aim: To analyze and quantify the 2018 dengue outbreak which occurred in district Shangla, Pakistan. Materials & methods: 964 suspected dengue samples were collected and examined for clinical manifestation and laboratory markers. Results: In all, 375 suspected cases were confirmed with dengue virus infection using nonstructural protein 1 (NS1) antigen, immunoglobulin M (IgM) & Immunoglobulin G (IgG) antibodies and real-time PCR whereas PCR was 92.2% sensitive. The most prevalent serotype was dengue virus 3 (60.26%). The male/female ratio was 1.84 and the most highly affected tehsil was Alpuri. The most affected age group was 16–40 years (70.4%). A significant number of cases were reported in September (48.54%). Conclusion: Recurrence of the dengue outbreaks in the study area could alarmingly increase the mortality rate, therefore, proper measures are essential to control dengue epidemics in the future.

The Use of Dengue Nonstructural Protein 1 Antigen for the Early Diagnosis During the Febrile Stage in Patients With Dengue Infection

2008 ◽  
Vol 27 (1) ◽  
pp. 43-48 ◽  
Author(s):  
Ampaiwan Chuansumrit ◽  
Wathanee Chaiyaratana ◽  
Viroj Pongthanapisith ◽  
Kanchana Tangnararatchakit ◽  
Sarapee Lertwongrath ◽  
...  
Keyword(s):  
Early Diagnosis ◽  
Nonstructural Protein ◽  
Dengue Infection ◽  
Nonstructural Protein 1

scholarly journals Coinfection of chikungunya and dengue viruses: A serological study from North Western region of Punjab, India

2018 ◽  
Vol 10 (04) ◽  
pp. 443-447 ◽  
Author(s):  
Maninder Kaur ◽  
Kanwardeep Singh ◽  
Shailpreet K Sidhu ◽  
Pushpa Devi ◽  
Manpreet Kaur ◽  
...  
Keyword(s):  
Nonstructural Protein ◽  
Dengue Infection ◽  
Signs And Symptoms ◽  
Control Measures ◽  
Health Concern ◽  
Serological Study ◽  
Serum Samples ◽  
Nonstructural Protein 1 ◽  
Antibody Capture ◽  
North Western

ABSTRACT INTRODUCTION: Dengue and chikungunya (CHIK) infections appear to be increasing in all parts of India. Aedes aegypti mosquitoes are common vectors for dengue virus (DENV) and CHIK virus (CHIKV). In areas where both viruses cocirculate, they can be transmitted together. There are very few studies discussing the dengue-chik coinfection from Punjab region of India. The present study was undertaken to study the clinical features of dengue–CHIK coinfection and compare with monoinfection. MATERIALS AND METHODS: IgM antibody capture (MAC) ELISA for dengue IgM and CHIK IgM and ELISA for nonstructural protein 1 antigen was performed on serum samples obtained from suspected patients. RESULTS: Out of total 3160 samples from suspected patients for dengue infection, 2178 (68.92%) samples were positive for DENV while CHIK IgM antibodies were positive in 127 patients out of the total suspected 373 cases (34.04%). In addition to this, 283 samples were tested for both viruses, out of which 27 sera were positive (9.54%) for coinfection of dengue and CHIK. The comparison of signs and symptoms showed that the coinfected patients had fever in all cases while rash was seen in only 30% cases. Arthralgia (79%) and thrombocytopenia (77%) was seen in significant number of coinfected cases thus revealing overlapping nature of dengue–CHIK coinfection. Conclusions: Increase in the number of Dengue and Chikungunya infections and their cocirculation is an important public health concern which warrants the implementation of strict control measures.

Assessing the effects of temperature on dengue transmission

2009 ◽  
Vol 137 (8) ◽  
pp. 1179-1187 ◽  
Author(s):  
H. M. YANG ◽  
M. L. G. MACORIS ◽  
K. C. GALVANI ◽  
M. T. M. ANDRIGHETTI ◽  
D. M. V. WANDERLEY
Keyword(s):  
Reproduction Number ◽  
Dengue Infection ◽  
Mass Action ◽  
Quantification Method ◽  
Dengue Transmission ◽  
Dengue Outbreaks ◽  
Different Temperatures ◽  
Vector Borne ◽  
Effects Of Temperature ◽  
The Basic Reproduction Number

SUMMARYThe incidence of dengue infection, a vector-borne disease transmitted by the mosquito Aedes aegypti, shows clear dependence on seasonal variation. Based on the quantification method that furnishes the size of the A. aegypti population in terms of the estimated entomological parameters for different temperatures, we assessed the risk of dengue outbreaks. The persistence and severity of epidemics can be assessed by the basic reproduction number R0, which varies with temperature. The expression for R0 obtained from ‘true’ and ‘pseudo’ mass action laws for dengue infection is discussed.

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