scholarly journals Employing Solid Phase Microextraction as Extraction Tool for Pesticide Residues in Traditional Medicinal Plants

2016 ◽  
Vol 2016 ◽  
pp. 1-11 ◽  
Author(s):  
Thamani T. Gondo ◽  
Veronica C. Obuseng ◽  
Lesego C. Mmualefe ◽  
Harriet Okatch
Keyword(s):  
Medicinal Plants ◽  
Solid Phase Microextraction ◽  
Solid Phase ◽  
Tsetse Fly ◽  
Plant Sample ◽  
Gas Chromatograph ◽  
Traditional Medicinal Plants ◽  
Control Disease ◽  
Ionisation Mass ◽  
Ionisation Mass Spectrometer

HS-SPME was optimised using blank plant sample for analysis of organochlorine pesticides (OCPs) of varying polarities in selected medicinal plants obtained from northern part of Botswana, where OCPs such as DDT and endosulfan have been historically applied to control disease carrying vectors (mosquitos and tsetse fly). The optimised SPME parameters were used to isolate analytes from root samples of five medicinal plants obtained from Maun and Kasane, Botswana. The final analytes determination was done with a gas chromatograph equipped with GC-ECD and analyte was confirmed using electron ionisation mass spectrometer (GC-MS). Dieldrin was the only pesticide detected and confirmed with MS in theTerminalia sericeasample obtained from Kasane. The method was validated and the analyte recoveries ranged from69.58±7.20to113±15.44%, with RSDs ranging from 1.19 to 17.97%. The method indicated good linearity (R2>0.9900) in the range of 2 to 100 ng g−1. The method also proved to be sensitive with low limits of detection (LODs) ranging from0.48±0.16to1.50±0.50 ng g−1. It can be concluded that SPME was successfully utilized as a sampling and extraction tool for pesticides of diverse polarities in root samples of medicinal plants.

Extraction and Phytochemicals Determination of Traditional Medicinal Plants for Anti-microbial Susceptibility Test

2020 ◽  
Vol 2020 ◽  
Author(s):  
Firew Admasu
Keyword(s):  
Antimicrobial Activity ◽  
Medicinal Plants ◽  
Traditional Medicine ◽  
Crude Extract ◽  
Susceptibility Test ◽  
Plant Sample ◽  
Traditional Medicinal Plants ◽  
Traditional Health Care ◽  
Susceptibility Tests

Abstract: The study were conducted at Dilla University, College of Natural Sciences, Biological Sciences laboratories. Background: Ethiopia is a country with many ethnic groups, cultures and beliefs which in turn have contributed to the high diversity of traditional health care knowledge and practices of traditional medicine from local growth plants, animals and minerals for various physical and mental disorders of human and livestock population that passed from generation to generation for centuries. Medicinal plants contributors to pharmaceutical, agricultural and food industries in the world. The use of medicinal plants in the industrialized societies has been traced to extraction and development of several drugs used in order to heel some diseases having inhibiting effect against pathogenic microorganism. Objective: The main objective of this study was Extraction and Phytochemicals determination of traditional medicinal plants for anti microbial susceptibility test. Methodology: The extraction and identification of some phytochemicals crude compound which used for antimicrobial susceptibility test from plant sample such as Ocimum lamiifolium (OL), Croton maerosth (Cm) and Ruta chalepesis (RC) were conducted. Plant samples are collected, powdered using mortal and pistil and extracted using ethanol and some susceptibility tests were performed to identify some phytochemicals compound. Result: The main result of Antimicrobial activity test showed that the crude extract of OL has the highest zone of inhibition. The highest yield of crude extract (38.21%) was obtained from Croton maerosth (CM) which followed by Ruta chalepesis (RC) (32.43%). However, the lowest yield (28.37%) was obtained from Oscpmum lamifolium (OL). Conclusion: Traditional Medicine is used by many people to managing numerous conditions; it’s accessible and effective on antimicrobial activity. Therefore, it plays a significant role by reducing life-threatening ailments of people and other animals.

scholarly journals Reliability of fibres in solid-phase microextraction for routine analysis of the headspace of aromatic and medicinal plants

2007 ◽  
Vol 1152 (1-2) ◽  
pp. 138-149 ◽  
Author(s):  
Carlo Bicchi ◽  
Chiara Cordero ◽  
Erica Liberto ◽  
Barbara Sgorbini ◽  
Patrizia Rubiolo
Keyword(s):  
Medicinal Plants ◽  
Solid Phase Microextraction ◽  
Solid Phase ◽  
Routine Analysis ◽  
Aromatic And Medicinal Plants

Combined headspace single-drop microextraction and solid-phase microextraction for the determination of phenols as their methyl ethers by gas chromatography-mass spectrometry

2017 ◽  
Vol 9 (46) ◽  
pp. 6590-6598 ◽  
Author(s):  
Archana Jain ◽  
Soumitra Soni ◽  
Kishan Reddy-Noone ◽  
Aradhana K. K. V. Pillai ◽  
Krishna K. Verma
Keyword(s):  
Solid Phase Microextraction ◽  
Solid Phase ◽  
High Sensitivity ◽  
Large Mass ◽  
Gas Chromatography Mass Spectrometry ◽  
Gas Chromatograph ◽  
Single Drop ◽  
Single Drop Microextraction ◽  
Single Drop Extraction

A combination of headspace large volume single drop extraction and solid phase microextraction permitted attainment of high sensitivity by transferring an overall large mass of analytes into a gas chromatograph.

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