scholarly journals Hb A1c Separation by High Performance Liquid Chromatography in Hemoglobinopathies

Scientifica ◽  
2016 ◽  
Vol 2016 ◽  
pp. 1-4 ◽  
Author(s):  
Vani Chandrashekar
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Rank Correlation ◽  
Beta Thalassemia ◽  
Compound Heterozygous ◽  
Hb A1c ◽  
Spearman’S Rank Correlation ◽  
Significant Difference ◽  
The Difference

Hb A1c measurement is subject to interference by hemoglobin traits and this is dependent on the method used for determination. In this paper we studied the difference between Hb A1c measured by HPLC in hemoglobin traits and normal chromatograms. We also studied the correlation of Hb A1c with age. Hemoglobin analysis was carried out by high performance liquid chromatography. Spearman’s rank correlation was used to study correlation between A1c levels and age. Mann-WhitneyUtest was used to study the difference in Hb A1c between patients with normal hemoglobin and hemoglobin traits. A total of 431 patients were studied. There was positive correlation with age in patients with normal chromatograms only. No correlation was seen in Hb E trait or beta thalassemia trait. No significant difference in Hb A1c of patients with normal chromatograms and patients with hemoglobin traits was seen. There is no interference by abnormal hemoglobin in the detection of A1c by high performance liquid chromatography. This method cannot be used for detection of A1c in compound heterozygous and homozygous disorders.

Unintended Reporting of Misleading Hb A1c Values When Using Assays Incapable of Detecting Hemoglobin Variants

2013 ◽  
Vol 137 (12) ◽  
pp. 1788-1791 ◽  
Author(s):  
Jeanne M. Rhea ◽  
David Koch ◽  
James Ritchie ◽  
Harsh V. Singh ◽  
Andrew N. Young ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Compound Heterozygous ◽  
Hb A1c ◽  
Cell Life ◽  
High Prevalence ◽  
Hb S ◽  
Hemoglobin Variants ◽  
Hb Variant

Context.—It is recommended that hemoglobin (Hb) A1c (Hb A1c) not be used to assess average glycemia in patients who have altered red blood cell life span. Objective.—To investigate the frequency of reporting an Hb A1c value for Hb variant samples that do not include Hb A. Design.—Hb A1c samples (n = 500) were procured and screened for Hb variants that may affect Hb A1c interpretation (Hb SS, Hb SC, and Hb S–β-thalassemia). Five of each of these samples were tested by ion-exchange high-performance liquid chromatography, immunoturbidimetric assay, second-generation immunoturbidimetric assay, and affinity chromatography. Results.—Eleven (2.2%) homozygous Hb SS, 6 (1.2%) Hb SC, and 5 (1.0%) Hb S–β-thalassemia samples were identified out of the 500 samples tested. Three of 4 instruments investigated in this study are known to not be plagued by analytic interference from these Hb variants but disturbingly reported Hb A1c values in the absence of Hb A. Conclusions.—The improved analytic specificity of Hb A1c platforms has by and large eliminated interferences from the most common heterozygous Hb variants. A consequence, however, is the potential for unintended reporting of Hb A1c results in the presence of homozygous and compound heterozygous Hb variants that lack Hb A and the inability to distinguish those samples not recommended to be used for patient care. The ability to identify samples harboring Hb variants that preclude the utility of Hb A1c may be beneficial in high prevalence populations.

scholarly journals Determination of Oxalates in Corms of Selected Taro (Colocasia esculenta) Varieties in Malaysia Using Ultra High-Performance Liquid Chromatography

2020 ◽  
pp. 28-37
Author(s):  
A. Mohd Zulkhairi ◽  
M. Razali ◽  
M. B. Umikalsum ◽  
G. Mohd Norfaizal ◽  
A. Aimi Athirah ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Dry Weight ◽  
Peninsular Malaysia ◽  
Colocasia Esculenta ◽  
Uv Detector ◽  
Commercial Cultivation ◽  
Significant Difference ◽  
The Difference

Aims: To determine the oxalate contents in different varieties of taro (Colocasia esculenta) collected in Peninsular Malaysia. Study Design: Ultra High-Performance Liquid Chromatography (UHPLC) with UV detector (Diode Array Detection, (DAD)) was used to determine the total and soluble oxalate contents in different varieties of taro corms. Meanwhile, the insoluble oxalate content (calcium oxalate) was estimated from the subtraction of soluble oxalate content from total oxalate content. Place and Duration of Study: Malaysian Agriculture Research and Development Institute (MARDI Headquarters), Persiaran MARDI-UPM, 43400 Serdang, Selangor, Malaysia between December 2018 to December 2019. Methodology: 9 different varieties of taro were collected from different locations in Peninsular Malaysia. All the samples were analysed for their oxalate contents. Extractions were carried out to determine the total oxalate and soluble oxalate contents. All the samples were analysed using UHPLC. The generated data of oxalate contents were analysed using Analysis of Variance (ANOVA). Results: There is a significant difference (P <.05) between the oxalate content in the examined varieties with respect to the amount of total, soluble and insoluble oxalate contents. The putih variety has significantly the highest amount of total oxalate content with 218.8 ± 28.2 mg/100 g DW (dry weight) followed by the udang variety with 184.2 ± 24.7 mg/100 g DW and the wangi variety with 178.3 ± 5.1 mg/100 g DW. Tapak badak variety has the lowest total oxalate content with 70.5 ± 20.1 mg/100 g DW. Result showed that wangi variety has significantly the highest soluble oxalate content with 135.1 ± 4.8 mg/100 g DW followed by udang with 100.9 ± 49.8 mg/100 g DW. The lowest soluble oxalate content was found in tapak badak with 17.7 ± 2.9 mg/100 g DW. Conclusion: Despite many factors contributing to the difference in oxalate content between varieties, this study would help researchers or policy makers to suggest potential taros for commercial cultivation.

scholarly journals Influence of spray mixture volume and flight height on herbicide deposition in aerial applications on pastures

2014 ◽  
Vol 32 (1) ◽  
pp. 227-232 ◽  
Author(s):  
M.A.P. Oliveira ◽  
U.R. Antuniassi ◽  
E.D. Velini ◽  
R.B. Oliveira ◽  
J.F. Salvador ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Mineral Oil ◽  
Mato Grosso ◽  
Significant Difference ◽  
Flight Height ◽  
Mixture Volume ◽  
Pasture Area ◽  
Experimental Plots

The objective of the present study was to analyze the influence of spray mixture volume and flight height on herbicide deposition in aerial applications on pastures. The experimental plots were arranged in a pasture area in the district of Porto Esperidião (Mato Grosso, Brazil). In all of the treatments, the applications contained the herbicides aminopyralid and fluroxypyr (Dominum) at the dose of 2.5 L c.p. ha-1, including the adjuvant mineral oil (Joint Oil) at the dose of 1.0 L and a tracer to determine the deposition by high-performance liquid chromatography (HPLC) (rhodamine at a concentration of 0.6%). The experiment consisted of nine treatments that comprised the combinations of three spray volumes (20, 30 and 50 L ha-1) and three flight heights (10, 30 and 40 m). The results showed that, on average, there was a tendency for larger deposits for the smallest flight heights, with a significant difference between the heights of 10 and 40 m. There was no significant difference among the deposits obtained with the different spray mixture volumes.

Differential intestinal deconjugation of taurine and glycine bile acid N-acyl amidates in rats

1992 ◽  
Vol 262 (2) ◽  
pp. G351-G358
Author(s):  
R. Zhang ◽  
S. Barnes ◽  
R. B. Diasio
Keyword(s):  
High Performance Liquid Chromatography ◽  
Small Intestine ◽  
Liquid Chromatography ◽  
Bile Acid ◽  
Chenodeoxycholic Acid ◽  
High Performance ◽  
Biliary Fistula ◽  
The Difference ◽  
The Stability ◽  
Rat Bile

Mechanisms responsible for the difference in the relative amounts of taurine- and glycine-conjugated bile acid N-acyl amidates (Tau/Gly ratio) are not fully understood. In the present study, the stability of taurine- and glycine-conjugated bile acid N-acyl amidates during intestinal transit and absorption was examined to investigate the contribution of intestinal deconjugation to the Tau/Gly ratio in rat bile. Radiolabeled chenodeoxycholic acid (CDC) and its N-acyl amidates with glycine (CDC-Gly) or taurine (CDC-Tau) were introduced into the lumen of the upper small intestine in the biliary fistula rats, and radioactive metabolites in bile, blood, urine, and tissues were identified and quantitated by high-performance liquid chromatography. Results indicated that 1) extensive deconjugation of CDC-Gly occurs during intestinal absorption; 2) CDC-Tau is recovered in bile largely intact; and 3) newly synthesized CDC-Tau and CDC-Gly are formed in a ratio of less than 2:1 after administration of [14C]-CDC. In summary, the present study demonstrates that resistance of taurine-conjugated bile acid N-acyl amidates to hydrolysis in the intestine, rather than a difference in synthesis of taurine- and glycine-conjugated N-acyl amidates in liver, may account for the high Tau/Gly ratio in rat bile.

scholarly journals Analysis of the High-Performance Liquid Chromatography Fingerprints and Quantitative Analysis of Multicomponents by Single Marker of Products of Fermented Cordyceps sinensis

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Li-hua Chen ◽  
Yao Wu ◽  
Yong-mei Guan ◽  
Chen Jin ◽  
Wei-feng Zhu ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Quantitative Analysis ◽  
High Performance ◽  
Cordyceps Sinensis ◽  
Array Detector ◽  
Hplc Fingerprint ◽  
External Standard Method ◽  
Significant Difference ◽  
Single Marker

Fermented Cordyceps sinensis, the succedaneum of Cordyceps sinensis which is extracted and separated from Cordyceps sinensis by artificial fermentation, is commonly used in eastern Asia in clinical treatments due to its health benefit. In this paper, a new strategy for differentiating and comprehensively evaluating the quality of products of fermented Cordyceps sinensis has been established, based on high-performance liquid chromatography (HPLC) fingerprint analysis combined with similar analysis (SA), hierarchical cluster analysis (HCA), and the quantitative analysis of multicomponents by single marker (QAMS). Ten common peaks were collected and analysed using SA, HCA, and QAMS. These methods indicated that 30 fermented Cordyceps sinensis samples could be categorized into two groups by HCA. Five peaks were identified as uracil, uridine, adenine, guanosine, and adenosine, and according to the results from the diode array detector, which can be used to confirm peak purity, the purities of these compounds were greater than 990. Adenosine was chosen as the internal reference substance. The relative correction factors (RCF) between adenosine and the other four nucleosides were calculated and investigated using the QAMS method. Meanwhile, the accuracy of the QAMS method was confirmed by comparing the results of that method with those of an external standard method with cosines of the angles between the groups. No significant difference between the two methods was observed. In conclusion, the method established herein was efficient, successful in identifying the products of fermented Cordyceps sinensis, and scientifically valid to be applicable in the systematic quality control of fermented Cordyceps sinensis products.

scholarly journals Determination of haemoglobin A1c levels using high-performance liquid chromatography of bloodstains

2019 ◽  
Vol 60 (1) ◽  
pp. 19-25
Author(s):  
Kemalettin Acar ◽  
Ayse Kurtulus Dereli ◽  
Esin Avci ◽  
Volkan Zeybek ◽  
Erdi Kutlu ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
High Reliability ◽  
Receiver Operating Curve ◽  
Haemoglobin A1c ◽  
Receiver Operating Curve Analysis ◽  
Significant Difference ◽  
Glass Surfaces ◽  
Hba1c Levels

This study aimed to determine haemoglobin A1c (HbA1c) levels in bloodstains shed on glass and fabric surfaces on specified test dates. Blood samples were taken from 26 patients (13 diabetic and 13 non-diabetic). Initial HbA1c levels were detected by using high-performance liquid chromatography (HPLC), and bloodstains were created on both cotton fabric and glass surfaces. Samples were processed at different ages (0, 7, 14, 28 and 56 days) by diluting distilled water and then measuring HbA1c levels by HPLC again. In all stains, HbA1c levels could be determined by using HPLC, but there was a moderate rise in accordance with the age of the stains. A statistically significant difference was found for bloodstains on clothes compared to those on glass surfaces. Receiver operating curve analysis found a sensitivity of 1.0 and specificity of 0.923 (cut-off 6.55) for glass surfaces on the seventh day; a sensitivity of 1.0, a specificity of 0.846 (cut-off 6.45) for clothes on the seventh day; a sensitivity of 1.0 and a specificity of 0.923 (cut-off 6.85) for clothes on the 56th day; and a sensitivity of 1.0 and a specificity of 0.846 (cut-off 7.55) for glass surfaces on the 56th day. In conclusion, this study found that HbA1c levels could be measured with high reliability from forensic bloodstains by using HPLC. Thus, in cases where DNA data banks cannot identify individuals, it would make sense to turn to those who have a medical history of diabetes among the suspects with the results of high HbA1c levels.

scholarly journals Investigation of eluted monomers from resin-based root canal sealer by high-performance liquid chromatography analysis

2016 ◽  
Vol 10 (01) ◽  
pp. 092-096 ◽  
Author(s):  
Huma Omurlu ◽  
Hacer Deniz Arisu ◽  
Evrim Eliguzeloglu Dalkilic ◽  
Ugur Tamer ◽  
Hilal Torul
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Bisphenol A ◽  
Root Canal ◽  
High Performance ◽  
Light Emitting Diode ◽  
Root Canal Sealer ◽  
Chromatography Analysis ◽  
Significant Difference ◽  
Time Periods

ABSTRACT Objective: The purpose of the current study was to determine the amount of urethane dimethacrylate (UDMA), bisphenol A-glycidyl methacrylate (Bis-GMA), poly (ethylene glycol) dimethacrylate (PEGDMA), bisphenol A ethoxylated dimethacrylate (Bis-EMA), and 2-hydroxyethyl methacrylate (HEMA) eluted from resin-based root canal sealer, epiphany, using high-performance liquid chromatography (HPLC). Materials and Methods: Epiphany was placed into the plastic molds and light-cured with a light emitting diode. After the curing process, each specimen in the first group (n = 12) was immersed in Eppendorf tubes containing a phosphate-buffered saline solution (PBS) and incubated for 45 s. In the second group, each specimen (n = 12) was immersed in Eppendorf tubes containing PBS and incubated for 24 h. Of the specimen extracts, 100 μL were subjected to HPLC. Analysis of data was accomplished with one-way analysis of variance (P < 0.05). Results: All of the samples eluted HEMA, UDMA, Bis-GMA, PEGDMA, and Bis-EMA. A significant difference was determined between the time periods of HEMA, UDMA, PEGDMA, and Bis-EMA (P < 0.05). Conclusion: The results of the current study showed that Epiphany releases HEMA, UDMA, Bis-GMA, PEGDMA, and Bis-EMA in both time periods.

scholarly journals Elimination of Bilirubin Interference in Creatinine Assays by Routine Techniques: Comparisons with a High Performance Liquid Chromatography Method

1994 ◽  
Vol 31 (3) ◽  
pp. 262-266 ◽  
Author(s):  
Sandra Boot ◽  
Nicholas LaRoche ◽  
Edward F Legg
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Reference Method ◽  
Chromatography Method ◽  
The Difference ◽  
Liquid Chromatography Method ◽  
Ideal Method ◽  
Single Slide ◽  
Blank Correction

The aim of this study was to find a method for creatinine analysis unaffected by jaundice by comparing four different methods with high performance liquid chromatography (HPLC) as a reference method. The methods investigated were Kodak DTSC single slide, Boehringer alkaline picrate with and without blank correction and a Boehringer enzymatic method, the last three using the Hitachi 737 analyser. HPLC results were compared with the results of the methods studied. In addition, graphs plotting the difference from the HPLC result against the bilirubin concentration were made; the ideal method would yield a slope and intercept of zero. While all methods showed a positive bias compared with HPLC, the Kodak slide and the Boehringer alkaline picrate with blank correction methods eliminated bilirubin interference and we have changed to the latter method as a consequence of this work.

scholarly journals Comparative Studies on the pH Dependence of DOWof Microcystin-RR and -LR Using LC-MS

2011 ◽  
Vol 11 ◽  
pp. 20-26 ◽  
Author(s):  
Gaodao Liang ◽  
Ping Xie ◽  
Jun Chen ◽  
Ting Yu
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Physicochemical Properties ◽  
Distribution Ratio ◽  
High Performance ◽  
Water Distribution ◽  
Ph Dependence ◽  
Remarkable Difference ◽  
The Difference

Microcystins (MCs) are well known worldwide as hepatotoxins produced by cyanobacteria, but little is known about the physicochemical properties of these compounds. The dependence of the n-octanol/water distribution ratio (DOW) of MC-RR and -LR to pH was measured by high-performance liquid chromatography combined with mass spectrometry (LC-MS). There was a remarkable difference in such relationships between MC-RR and -LR. The log DOWof MC-LR decreased from 1.63 at pH 1.0 to -1.26 at pH 6.5, and stabilized between -1.04 and -1.56 at a pH of 6.5~12.0; log DOWof MC-RR varied between -1.24 and -0.67 at a pH of 1.00~4.00, and stabilized between -1.20 and -1.54 at a pH of 4.00~12.00. The difference of hydrophobicity in acidic condition between MC-RR and -LR is important, not only for the analytical method of both toxins, but perhaps also for understanding the difference of toxicity to animals between the two toxins.

scholarly journals EFFECT OF DIFFERENT ANTICOAGULANTS IN DETERMINING METFORMIN HYDROCHLORIDE LEVELS IN HUMAN PLASMA USING HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY

2018 ◽  
Vol 10 (1) ◽  
pp. 148
Author(s):  
Yahdiana Harahap ◽  
Lista Roro Marsudi ◽  
Sunarsih .
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Human Plasma ◽  
High Performance ◽  
Ethylenediaminetetraacetic Acid ◽  
Sodium Dodecyl ◽  
Internal Standard ◽  
Array Detector ◽  
Metformin Hydrochloride ◽  
Significant Difference

Objective: This study aimed to develop and validate an analytical method and to determine the effect of different anticoagulants for analyzingmetformin HCl level in human plasma.Methods: Analysis was performed with high-performance liquid chromatography method using a photodiode array detector set at 234 nm, a C18SunFire™ column (5 μm, 250 mm×4.6 mm), a temperature of 40°C, a mobile phase comprising 10 mM sodium dodecyl sulfate and 10 mM phosphatebuffer in water-acetonitrile (60:40 v/v) at pH 7.0, with a flow rate of 1.0 mL/min, and atorvastatin calcium as the internal standard. Plasmaextraction was performed through protein precipitation using human plasma (300 μL) and acetonitrile (600 μL; 1:2 v/v). The method was linear at aconcentration range of 20.0–5000.0 ng/mL, with r>0.9998.Results: The stability and recovery of metformin HCl in human plasma were not different between citrate with ethylenediaminetetraacetic acid(EDTA) and heparin with EDTA as anticoagulants. However, a significant difference in the peak area response ratio (p<0.05) was observed betweenthe three anticoagulants.Conclusion: The validation results for the three types of plasma anticoagulants met validation requirements based on the European MedicinesAgency bioanalytical guidelines of 2011 for accuracy and precision, selectivity, calibration curve linearity, dilution integrity, carry-over, and stability.

Sign in / Sign up

Export Citation Format

Share Document