scholarly journals Determination of Alcohol Content in Alcoholic Beverages Using 45 MHz Benchtop NMR Spectrometer

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Meden F. Isaac-Lam
Keyword(s):  
Acetic Acid ◽  
Analytical Methods ◽  
Internal Standard ◽  
Standard Addition ◽  
Alcoholic Beverages ◽  
Internal Standard Method ◽  
Alcohol Content ◽  
Nuclear Magnetic Resonance Spectrometer ◽  
Low Field ◽  
Ethanol Acetic Acid

Alcohol or ethanol is considered the most widely used recreational drug worldwide, and its production, consumption, and sale are strictly regulated by laws. Alcohol content of alcoholic beverages (wine, beers, and spirits) is about 3–50% v/v. Analytical methods to determine the alcohol content must be reliable, precise, and accurate. In this study, the amount of ethanol in several alcoholic beverages was determined using a 45 MHz low-field benchtop NMR (nuclear magnetic resonance) spectrometer. Internal standard and standard addition analytical methods were utilized to quantify ethanol. For both methods, acetic acid or acetonitrile was used as internal standard to quantify alcohol content by using the peak area corresponding to the methyl peaks of ethanol, acetic acid, or acetonitrile. Results showed that internal standard method gave values of percent alcohol that are in close agreement with the indicated label as confirmed by running the samples in a 400 MHz high-field NMR spectrometer using acetic acid as internal standard. This study demonstrates the utility of a benchtop NMR spectrometer that can provide an alternative technique to analyze percent alcohol in alcoholic products.

scholarly journals Ginsenosides in Commercial Ginseng Products Analyzed by Liquid Chromatography-Tandem Mass Spectrometry

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Linda Uhr ◽  
Yuchun Chen ◽  
Daniel Sit ◽  
Paul C. H. Li
Keyword(s):  
Standard Method ◽  
Internal Standard ◽  
Standard Addition ◽  
Standard Addition Method ◽  
Tandem Mass ◽  
Internal Standard Method ◽  
Addition Method ◽  
Standard Methods ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass

Although the LC/MS-MS method has been developed to analyze ginsenosides in ginseng roots, this method has not yet been utilized to quantify ginsenosides in ginseng products. In this paper, an internal standard method by LC/MS-MS was developed to analyze the ginsenosides content in the commercial ginseng products such as the ginseng capsules. We compare the standard addition method and the internal standard methods for the quantification of several ginsenosides (e.g., Rb1, Rb2, Rb3, Rc, Rd, Re, Rg1, and Rg3) in five commercial ginseng capsules. The results showed that the internal standard method is as good as the standard addition method and is a less tedious approach.

scholarly journals Confirmation of the suitability of the method of using ethanol as a reference substance for quantitative determination of volatile compounds in alcoholic beverages

2020 ◽  
Vol 64 (12) ◽  
pp. 60-75
Author(s):  
Sergey V. Cherepitsa ◽  
◽  
Svetlana N. Sytova ◽  
Lidia N. Sobolenko ◽  
Evgeny D. Shevchenko ◽  
...  
Keyword(s):  
Internal Standard ◽  
Relative Difference ◽  
Alcoholic Beverages ◽  
Volatile Components ◽  
Internal Standard Method ◽  
Reference Substance ◽  
Safety Control ◽  
Acetate Methyl ◽  
Wide Range

Quality and safety control of alcohol products is an integral part of the work of laboratories for the food analysis. One of the most important safety indicators regulated worldwide is the chemical composition of an alcoholic beverages, namely the presence of characteristic and impurity volatile components. The list of controlled compounds includes acetaldehyde, methyl acetate, ethyl acetate, methyl, propyl, isopropyl, butyl, isobutyl and isoamyl alcohols, etc. Analysis of alcoholic beverages is a conservative field, since its results are closely associated with economic risks for the manufacturer, and also directly with the health of the population consuming this product. For this reason, the method, used for the analysis of alcoholic beverages, should be accurate, reproducible and simple for use. The authors have developed and described a method that satisfies the above requirements, based on the use of ethanol containing in alcoholic beverages as a reference substance for gas chromatographic analysis. Confirmation of the suitability of the proposed method for the analysis of a wide range of alcoholic beverages: whiskey, brandy, grappa, vodka, scotch tape, bourbon, brandy, calvados, sake, alcohol, rum, gin and tequila, is presented. The results, obtained by the proposed method are compared with the results obtained by the generally accepted traditional method of the internal standard using 2-pentanol. The repeatability and trueness of the both methods are evaluated. The relative difference between the results, obtained by the internal standard method, and the developed method did not exceed 2%.

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