scholarly journals Localisation of Lactate Transporters in Rat and Rabbit Placentae

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Nigel P. Moore ◽  
Catherine A. Picut ◽  
Jeffrey H. Charlap
Keyword(s):  
Red Blood Cells ◽  
Blood Vessels ◽  
Blood Cells ◽  
Opposite Polarity ◽  
Monocarboxylate Transporter ◽  
Sprague Dawley ◽  
Nucleated Red Blood Cells ◽  
Sprague Dawley Rats ◽  
New Zealand White Rabbits ◽  
Pyruvic Acids

The distribution of monocarboxylate transporter (MCT) isoforms 1 and 4, which mediate the plasmalemmal transport of l-lactic and pyruvic acids, has been identified in the placentae of rats and rabbits at different ages of gestation. Groups of three pregnant Sprague-Dawley rats and New Zealand White rabbits were sacrificed on gestation days (GD) 11, 14, 18, or 20 and on GD 13, 18, or 28, respectively. Placentae were removed and processed for immunohistochemical detection of MCT1 and MCT4. In the rat, staining for MCT1 was associated with lakes and blood vessels containing enucleated red blood cells (maternal vessels) while staining for MCT4 was associated with vessels containing nucleated red blood cells (embryofoetal vessels). In the rabbit, staining for MCT1 was associated with blood vessels containing nucleated red blood cells while staining for MCT4 was associated with vessels containing enucleated red blood cells. Strength of staining for MCT1 decreased during gestation in both species, but that for MCT4 was stronger than that for MCT1 and was consistent between gestation days. The results imply an opposite polarity of MCT1 and MCT4 across the trophoblast between rat and rabbit.

scholarly journals THE PATHOGENESIS OF ERYTHROBLASTOSIS FETALIS

Blood ◽  
1949 ◽  
Vol 4 (11) ◽  
pp. 1249-1255 ◽  
Author(s):  
B. S. KLINE
Keyword(s):  
Red Blood Cells ◽  
Blood Vessels ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Maternal Blood ◽  
Fetal Blood ◽  
Fetal Circulation ◽  
Nucleated Red Blood Cells

Abstract The mechanism of transfer, in cases of erythroblastosis fetalis, of incompatible fetal red blood cells to the mother and of maternal blood with antibody to the fetus, was observed especially well in 2 cases in which the infants were born alive. The two placentas showed occlusion of peripheral blood vessels of many villi and trunks by agglutinated red blood cells and fibrin. Associated with the vascular thromboses, there were, in places, necrosis of the walls and of regional tissues with rupture and hemorrhage of fetal blood, containing numerous intact nucleated red blood cells, into regional intervillous spaces. Through the broken surfaces, adjacent maternal blood was in contact with the fetal circulation. A more accurate designation for "erythroblastosis fetalis" would be "transplacental erythrocytotoxic anemia."

scholarly journals Toxic effects of Litsea elliptica Blume essential oil on red blood cells of Sprague-Dawley rats

2009 ◽  
Vol 10 (11) ◽  
pp. 813-819 ◽  
Author(s):  
Izatus Shima Taib ◽  
Siti Balkis Budin ◽  
Seri Maseran Siti Nor Ain ◽  
Jamaludin Mohamed ◽  
Santhana Raj Louis ◽  
...  
Keyword(s):  
Essential Oil ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Toxic Effects ◽  
Sprague Dawley ◽  
Sprague Dawley Rats

Peliosis Hepatis-like Lesion in Aging Rats

1983 ◽  
Vol 20 (4) ◽  
pp. 410-423 ◽  
Author(s):  
K. P. Lee
Keyword(s):  
Endothelial Cells ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Hepatic Cell ◽  
Degenerative Changes ◽  
Peliosis Hepatis ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Membrane Bound ◽  
Membranous Wall

The incidence of peliosis hepais-like lesions in two-year-old Charles River-CD Sprague-Dawley rats (Charles River Breeding laboratories, Wilmington, MA) was almost twice as high in males as females. Blood lakes developed from progressive expansion of sinusoids with destruction of the hepatic cell cords. The endothelial processes of the blood lakes had edematous swelling, vesiculation, and denudation. The microvilli of hepatocytes became swollen, blunted, and sloughed where the endothelial processes were disrupted. Subsequently, the hepatocytes were exposed directly to circulating blood cells and had degenerative changes with accumulation of red blood cells in the cytoplasm. The membrane-bound cysts were observed with the blood lakes and were bounded with atrophic hepatocytes or membranous wall extending to the Disse's space of adjacent hepatocytes. The cysts were filled with proteinaccous fluid, fibrin, and a few red blood cells. The wall of membrane-bound cysts consisted of endothelial processes extending from the endothelial cells of adjacent hepatocytes. The wall appeared to develop from adhesion of endothelial processes surrounding sinusoidal spaces of the hepatocytes following lysis of atrophic hepatocytes.

Nucleated red blood cells and hematological scoring system – Future trends in early onset neonatal sepsis

2019 ◽  
Vol 12 (1) ◽  
pp. 53-58
Author(s):  
Shivendra Vikram Singh ◽  
◽  
Megalamane Supreetha ◽  
Satyavathi R Alva ◽  
◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Neonatal Sepsis ◽  
Blood Cells ◽  
Early Onset ◽  
Scoring System ◽  
Future Trends ◽  
Nucleated Red Blood Cells

Nucleated Red Blood Cells as Markers of Perinatal Adaptation in Preterm Neonates Receiving Minimally Invasive Surfactant Therapy

2021 ◽  
Author(s):  
Itamar Nitzan ◽  
Calum T. Roberts ◽  
Risha Bhatia ◽  
Francis B. Mimouni ◽  
Arvind Sehgal
Keyword(s):  
Minimally Invasive ◽  
Right Ventricular ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Surfactant Therapy ◽  
Preterm Neonates ◽  
Oxygen Requirement ◽  
Nucleated Red Blood Cells ◽  
Intrauterine Hypoxia ◽  
Ventricular Ejection Time

Objective The study aimed to assess the association of nucleated red blood cells (NRBC), a surrogate of intrauterine hypoxia, and elevated pulmonic vascular resistance (E-PVR) and oxygen requirement after minimally invasive surfactant therapy (MIST). Study Design Retrospective study of a cohort of preterm neonates that received MIST in a single unit. Results NRBC were measured in 65 of 75 (87%) neonates administered MIST during the period. In total, 22 of 65 (34%) infants had pre-MIST echocardiography (ECHO).Neonates with elevated NRBC (predefined as >5 × 109/L, n = 16) required higher post-MIST fraction of inspired oxygen (FiO2) than neonates with normal NRBC (<1 × 109/L, n = 17; FiO2 = 0.31 ± 0.10 and 0.24 ± 0.04, respectively, p = 0.02).NRBC correlated positively with % of time in right to left ductal shunt (r = 0.51, p = 0.052) and inversely with right ventricular stroke volume (r = −0.55, p = 0.031) and time to peak velocity to right ventricular ejection time ratio (r = −0.62, p < 0.001). Conclusion Elevated NRBC are associated with elevated FiO2 after MIST and elevated E-PVR. Intrauterine hypoxia may impact postnatal circulatory adaptations and oxygen requirement. Key Points

Nucleated red blood cells participate in myocardial regeneration in the toad Bufo Gargarizan Gargarizan

2021 ◽  
pp. 153537022110132
Author(s):  
Shu-Qin Liu ◽  
Xiao-Ye Hou ◽  
Feng Zhao ◽  
Xiao-Ge Zhao
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Cardiac Injury ◽  
Myocardial Regeneration ◽  
Matrix Metalloproteinase 2 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cardiac Tissues ◽  
Nucleated Red Blood Cells ◽  
Model Animal

Heart regeneration is negligible in humans and mammals but remarkable in some ectotherms. Humans and mammals lack nucleated red blood cells (NRBCs), while ectotherms have sufficient NRBCs. This study used Bufo gargarizan gargarizan, a Chinese toad subspecies, as a model animal to verify our hypothesis that NRBCs participate in myocardial regeneration. NRBC infiltration into myocardium was seen in the healthy toad hearts. Heart needle-injury was used as an enlarged model of physiological cardiomyocyte loss. It recovered quickly and scarlessly. NRBC infiltration increased during the recovery. Transwell assay was done to in vitro explore effects of myocardial injury on NRBCs. In the transwell system, NRBCs could infiltrate into cardiac pieces and could transdifferentiate toward cardiomyocytes. Heart apex cautery caused approximately 5% of the ventricle to be injured to varying degrees. In the mildly to moderately injured regions, NRBC infiltration increased and myocardial regeneration started soon after the inflammatory response; the severely damaged region underwent inflammation, scarring, and vascularity before NRBC infiltration and myocardial regeneration, and recovered scarlessly in four months. NRBCs were seen in the newly formed myocardium. Enzyme-linked immunosorbent assay and Western blotting showed that the levels of tumor necrosis factor-α, interleukin- 1β, 6, and11, cardiotrophin-1, vascular endothelial growth factor, erythropoietin, matrix metalloproteinase- 2 and 9 in the serum and/or cardiac tissues fluctuated in different patterns during the cardiac injury-regeneration. Cardiotrophin-1 could induce toad NRBC transdifferentiation toward cardiomyocytes in vitro. Taken together, the results suggest that the NRBC is a cell source for cardiomyocyte renewal/regeneration in the toad; cardiomyocyte loss triggers a series of biological processes, facilitating NRBC infiltration and transition to cardiomyocytes. This finding may guide a new direction for improving human myocardial regeneration.

Emerging Microfluidic Technologies for the Detection of Circulating Tumor Cells and Fetal Nucleated Red Blood Cells

2021 ◽  
Vol 4 (2) ◽  
pp. 1140-1155
Author(s):  
Xiaoyun Wei ◽  
Keke Chen ◽  
Shishang Guo ◽  
Wei Liu ◽  
Xing-Zhong Zhao
Keyword(s):  
Red Blood Cells ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Blood Cells ◽  
Nucleated Red Blood Cells
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