scholarly journals A Two-Dimensional Numerical Investigation of Transport of Malaria-Infected Red Blood Cells in Stenotic Microchannels

2016 ◽  
Vol 2016 ◽  
pp. 1-16 ◽  
Author(s):  
Tong Wang ◽  
Yong Tao ◽  
Uwitije Rongin ◽  
Zhongwen Xing
Keyword(s):  
Blood Cell ◽  
Red Blood Cells ◽  
Red Blood Cell ◽  
Blood Cells ◽  
Flow Characteristics ◽  
Cell Stiffness ◽  
Immersed Boundary ◽  
Cell Aggregates ◽  
Two Dimensional ◽  
Cell Deformability

The malaria-infected red blood cells experience a significant decrease in cell deformability and increase in cell membrane adhesion. Blood hemodynamics in microvessels is significantly affected by the alteration of the mechanical property as well as the aggregation of parasitized red blood cells. In this study, we aim to numerically study the connection between cell-level mechanobiological properties of human red blood cells and related malaria disease state by investigating the transport of multiple red blood cell aggregates passing through microchannels with symmetric stenosis. Effects of stenosis magnitude, aggregation strength, and cell deformability on cell rheology and flow characteristics were studied by a two-dimensional model using the fictitious domain-immersed boundary method. The results indicated that the motion and dissociation of red blood cell aggregates were influenced by these factors and the flow resistance increases with the increase of aggregating strength and cell stiffness. Further, the roughness of the velocity profile was enhanced by cell aggregation, which considerably affected the blood flow characteristics. The study may assist us in understanding cellular-level mechanisms in disease development.

Susceptibility of density-fractionated erythrocytes to subhaemolytic mechanical shear stress

2018 ◽  
Vol 42 (3) ◽  
pp. 151-157 ◽  
Author(s):  
Antony P McNamee ◽  
Kieran Richardson ◽  
Jarod Horobin ◽  
Lennart Kuck ◽  
Michael J Simmonds
Keyword(s):  
Blood Cell ◽  
Red Blood Cells ◽  
Red Blood Cell ◽  
Blood Cells ◽  
Shear Stresses ◽  
Mechanical Stimuli ◽  
Cell Deformability ◽  
Red Blood Cell Deformability ◽  
Cell Age ◽  
Low Shear

Introduction: Accumulating evidence demonstrates that subhaemolytic mechanical stresses, typical of circulatory support, induce physical and biochemical changes to red blood cells. It remains unclear, however, whether cell age affects susceptibility to these mechanical forces. This study thus examined the sensitivity of density-fractionated red blood cells to sublethal mechanical stresses. Methods: Red blood cells were isolated and washed twice, with the least and most dense fractions being obtained following centrifugation (1500 g × 5 min). Red blood cell deformability was determined across an osmotic gradient and a range of shear stresses (0.3–50 Pa). Cell deformability was also quantified before and after 300 s exposure to shear stresses known to decrease (64 Pa) or increase (10 Pa) red blood cell deformability. The time course of accumulated sublethal damage that occurred during exposure to 64 Pa was also examined. Results: Dense red blood cells exhibited decreased capacity to deform when compared with less dense cells. Cellular response to mechanical stimuli was similar in trend for all red blood cells, independent of density; however, the magnitude of impairment in cell deformability was exacerbated in dense cells. Moreover, the rate of impairment in cellular deformability, induced by 64 Pa, was more rapid for dense cells. Relative improvement in red blood cell deformability, due to low-shear conditioning (10 Pa), was consistent for both cell populations. Conclusion: Red blood cell populations respond differently to mechanical stimuli: older (more dense) cells are highly susceptible to sublethal mechanical trauma, while cell age (density) does not appear to alter the magnitude of improved cell deformability following low-shear conditioning.

scholarly journals Deformability based sorting of stored red blood cells reveals donor-dependent aging curves

Lab on a Chip ◽  
2020 ◽  
Vol 20 (2) ◽  
pp. 226-235 ◽  
Author(s):  
Emel Islamzada ◽  
Kerryn Matthews ◽  
Quan Guo ◽  
Aline T. Santoso ◽  
Simon P. Duffy ◽  
...  
Keyword(s):  
Blood Cell ◽  
Red Blood Cells ◽  
Cold Storage ◽  
Red Blood Cell ◽  
Cell Sorting ◽  
Blood Cells ◽  
Cell Deformability ◽  
Red Blood Cell Deformability

Cell sorting using microfluidic ratchets enables sensitive and consistent characterization of donor red blood cell deformability. Using this capability, we show the degradation of red blood cell deformability during cold storage is donor-dependent.

scholarly journals Regulation of red blood cell filterability by Ca2+ influx and cAMP-mediated signaling pathways

1997 ◽  
Vol 273 (6) ◽  
pp. C1828-C1834 ◽  
Author(s):  
Tadahiro Oonishi ◽  
Kanako Sakashita ◽  
Nobuhiro Uyesaka
Keyword(s):  
Blood Cell ◽  
Red Blood Cells ◽  
Signaling Pathways ◽  
Mechanical Stress ◽  
Red Blood Cell ◽  
Blood Cells ◽  
Camp Level ◽  
Membrane Properties ◽  
Cell Deformability ◽  
Red Blood Cell Deformability

To investigate the mechanism of the regulation of human red blood cell deformability, we examined the deformability under mechanical stress. Washed human red blood cells were rapidly injected through a fine needle, and their filterability was measured using a nickel mesh filter. The decrease in filterability showed a V-shaped curve depending on the extracellular Ca2+ concentration; the maximum decrease was achieved at ∼50 μM. The decreased filterability was accompanied by no change in cell morphology and cell volume, indicating that the decrease in filterability can be ascribed to alterations of the membrane properties. Ca2+entry blockers (nifedipine and felodipine) inhibited the impairment of filterability under mechanical stress. Prostaglandins E1 and E2, epinephrine, and pentoxifylline, which are thought to modulate the intracellular adenosine 3′,5′-cyclic monophosphate (cAMP) level of red blood cells, improved or worsened the impaired filterability according to their expected actions on the cAMP level of the cells. These results strongly suggest that the membrane properties regulating red blood cell deformability are affected by the signal transduction system, including Ca2+-dependent and cAMP-mediated signaling pathways.

Assessing Red Blood Cell Deformability using Deep Learning

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Erik S. Lamoureux ◽  
Emel Islamzada ◽  
Matthew V.J. Wiens ◽  
Kerryn Matthews ◽  
Simon P. Duffy ◽  
...  
Keyword(s):  
Deep Learning ◽  
Blood Cell ◽  
Red Blood Cells ◽  
Red Blood Cell ◽  
Blood Cells ◽  
Natural Aging ◽  
Cell Deformability ◽  
Red Blood Cell Deformability ◽  
Rbc Deformability

Red blood cells (RBCs) must be highly deformable to transit through the microvasculature to deliver oxygen to tissues. The loss of RBC deformability resulting from pathology, natural aging, or storage...

Geometrical alignment for improving cell evaluation in a microchannel with application on multiple myeloma red blood cells

RSC Advances ◽  
2014 ◽  
Vol 4 (85) ◽  
pp. 45050-45058 ◽  
Author(s):  
Chia-Hung Dylan Tsai ◽  
Shinya Sakuma ◽  
Fumihito Arai ◽  
Tatsunori Taniguchi ◽  
Tomohito Ohtani ◽  
...  
Keyword(s):  
Multiple Myeloma ◽  
Blood Cell ◽  
Red Blood Cells ◽  
Red Blood Cell ◽  
Blood Cells ◽  
Cell Deformability ◽  
Red Blood Cell Deformability ◽  
Alignment Mechanism

A microfluidic design for evaluating red blood cell deformability with geometrical alignment mechanism is proposed.

scholarly journals The role of membrane skeletal-associated alpha-globin in the pathophysiology of beta-thalassemia

Blood ◽  
1990 ◽  
Vol 75 (6) ◽  
pp. 1333-1336 ◽  
Author(s):  
S Sorensen ◽  
E Rubin ◽  
H Polster ◽  
N Mohandas ◽  
S Schrier
Keyword(s):  
Transgenic Mice ◽  
Blood Cell ◽  
Red Blood Cells ◽  
Red Blood Cell ◽  
Blood Cells ◽  
Beta Thalassemia ◽  
Beta Globin ◽  
Cell Deformability ◽  
Red Blood Cell Deformability ◽  
Alpha Globin

The beta-thalassemic mouse provides a useful model for testing hypotheses about the pathophysiology in human beta-thalassemia. The clinical picture of these mice and their red blood cell deformability characteristics are quite similar to those observed in human beta- thalassemia intermedia. The creation of transgenic mice that express human beta-globin (beta s) has provided an opportunity to study the effect of increasing the non-alpha-globin chain production on the thalassemic phenotype. A small increase in beta-globin production produces transgenic mice that are healthier, have almost normal hemoglobin values, and whose red blood cell deformability is increased. We quantified and characterized the membrane skeletal-associated globin in normal, transgenic thal/sickle, and thalassemic mice and showed that only alpha-globin was associated with the membrane skeleton in the pathologic red blood cells, and that the degree of rigidity as measured in the rheoscope correlated directly and closely with the amount of membrane skeletal-associated globin in these abnormal red blood cells.

scholarly journals Molecular Rotors as intracellular probes of Red Blood Cell stiffness

Soft Matter ◽  
2021 ◽  
Author(s):  
Alice Briole ◽  
Thomas Podgorski ◽  
Berengere Abou
Keyword(s):  
Blood Cell ◽  
Red Blood Cells ◽  
Red Blood Cell ◽  
Blood Cells ◽  
The Body ◽  
Molecular Rotors ◽  
Cell Stiffness ◽  
Essential Parameter

The deformability of red blood cells is an essential parameter that controls the rheology of blood as well as its circulation in the body. Characterizing the rigidity of the cells...

scholarly journals The role of membrane skeletal-associated alpha-globin in the pathophysiology of beta-thalassemia

Blood ◽  
1990 ◽  
Vol 75 (6) ◽  
pp. 1333-1336 ◽  
Author(s):  
S Sorensen ◽  
E Rubin ◽  
H Polster ◽  
N Mohandas ◽  
S Schrier
Keyword(s):  
Transgenic Mice ◽  
Blood Cell ◽  
Red Blood Cells ◽  
Red Blood Cell ◽  
Blood Cells ◽  
Beta Thalassemia ◽  
Beta Globin ◽  
Cell Deformability ◽  
Red Blood Cell Deformability ◽  
Alpha Globin

Abstract The beta-thalassemic mouse provides a useful model for testing hypotheses about the pathophysiology in human beta-thalassemia. The clinical picture of these mice and their red blood cell deformability characteristics are quite similar to those observed in human beta- thalassemia intermedia. The creation of transgenic mice that express human beta-globin (beta s) has provided an opportunity to study the effect of increasing the non-alpha-globin chain production on the thalassemic phenotype. A small increase in beta-globin production produces transgenic mice that are healthier, have almost normal hemoglobin values, and whose red blood cell deformability is increased. We quantified and characterized the membrane skeletal-associated globin in normal, transgenic thal/sickle, and thalassemic mice and showed that only alpha-globin was associated with the membrane skeleton in the pathologic red blood cells, and that the degree of rigidity as measured in the rheoscope correlated directly and closely with the amount of membrane skeletal-associated globin in these abnormal red blood cells.

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