scholarly journals Optimized Stem Cell Detection Using the DyeCycle-Triggered Side Population Phenotype

2016 ◽  
Vol 2016 ◽  
pp. 1-14 ◽  
Author(s):  
Maximilian Boesch ◽  
Dominik Wolf ◽  
Sieghart Sopper
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Cancer Stem Cells ◽  
Cell Biology ◽  
Side Population ◽  
Blue Laser ◽  
Cell Detection ◽  
Target Populations ◽  
And Function ◽  
Transporter Expression

Tissue and cancer stem cells are highly attractive target populations for regenerative medicine and novel potentially curative anticancer therapeutics. In order to get a better understanding of stem cell biology and function, it is essential to reproducibly identify these stem cells from biological samples for subsequent characterization or isolation. ABC drug transporter expression is a hallmark of stem cells. This is utilized to identify (cancer) stem cells by exploiting their dye extrusion properties, which is referred to as the “side population assay.” Initially described for high-end flow cytometers equipped with ultraviolet lasers, this technique is now also amenable for a broader scientific community, owing to the increasing availability of violet laser-furnished cytometers and the advent of DyeCycle Violet (DCV). Here, we describe important technical aspects of the DCV-basedside population assayand discuss potential pitfalls and caveats helping scientists to establish a valid and reproducible DCV-basedside population assay. In addition, we investigate the suitability of blue laser-excitable DyeCycle dyes for side population detection. This knowledge will help to improve and standardize detection and isolation of stem cells based on their expression of ABC drug transporters.

Characterization of the Putative Stem Cells From Umbilical Cord Blood.

Blood ◽  
2009 ◽  
Vol 114 (22) ◽  
pp. 4231-4231
Author(s):  
Amos S. Gaikwad ◽  
Michael Cubbage ◽  
Tatiana Goltsova ◽  
Christopher Threeton ◽  
Maria Ty ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Cord Blood ◽  
Cell Population ◽  
Cell Biology ◽  
Mononuclear Cells ◽  
Conflicts Of Interest ◽  
Side Population ◽  
Hematopoietic Stem ◽  
Cell Fractions

Abstract Abstract 4231 Cord blood (CB) is a rich source of hematopoietic stem cells (HSC) with long-term repopulating activity necessary for allogeneic stem cell transplantation. CD34+ stem cells are considered sufficient for transplantation, however recent progress in stem cell biology indicates that cells with other surface markers such as CD133 or cells expressing high aldehyde dehydrogenase activity with low side scatter (ALDHhigh/SSClow) or a rare side population (SP) of cells that exclude the Hoechst 33342 vital dye via multi drug transporters have been shown to possess stem cell properties. We characterized CD34+, CD133+, ALDH+ and SP in mononuclear cells (MNC) isolated from human CB. While the SP cell population is rare and detectable in few CB-MNC examined, we found abundant CD34+ and CD133+ cells (1.0+/-0.5 and 0.8+/-0.4 per 100 CD45+ MNC cells, respectively) following the ISHAGE protocol. A distinct ALDH+ cell population (median of 0.26%; range of 0.1 to 0.5%) was also present in all of the CB-MNC analyzed. Over 90% of the ALDH+ cells were also CD34+ and CD133+. The ability of CB-MNC to form colonies in methocult semi-solid media supplemented with cytokines yielded myeloid, lymphoid and erythroid colonies. The clonogenic potential of CB-MNC ranged from 16-48%. We are assessing the colony forming ability of purified stem cell fractions using flow cytometry. The clonogenic efficiency of these individual putative stem cells will be discussed. Disclosures: No relevant conflicts of interest to declare.

scholarly journals Gastric Cancer Stem Cells

2008 ◽  
Vol 26 (17) ◽  
pp. 2876-2882 ◽  
Author(s):  
Shigeo Takaishi ◽  
Tomoyuki Okumura ◽  
Timothy C. Wang
Keyword(s):  
Gastric Cancer ◽  
Stem Cells ◽  
Stem Cell ◽  
Cancer Stem Cells ◽  
Cancer Stem Cell ◽  
Cell Biology ◽  
Cancer Biology ◽  
Human Cancer ◽  
Immunodeficient Mice

Cancer stem cells are defined as the unique subpopulation in the tumors that possess the ability to initiate tumor growth and sustain self-renewal as well as metastatic potential. Accumulating evidence in recent years strongly indicate the existence of cancer stem cells in solid tumors of a wide variety of organs. In this review, we will discuss the possible existence of a gastric cancer stem cell. Our recent data suggest that a subpopulation with a defined marker shows spheroid colony formation in serum-free media in vitro, as well as tumorigenic ability in immunodeficient mice in vivo. We will also discuss the possible origins of the gastric cancer stem cell from an organ-specific stem cell versus a recently recognized new candidate bone marrow–derived cell (BMDC). We have previously shown that BMDC contributed to malignant epithelial cells in the mouse model of Helicobacter-associated gastric cancer. On the basis of these findings from animal model, we propose that a similar phenomenon may also occur in human cancer biology, particularly in the cancer origin of other inflammation-associated cancers. The expanding research field of cancer stem-cell biology may offer a novel clinical apparatus to the diagnosis and treatment of cancer.

scholarly journals An NRF2 Perspective on Stem Cells and Ageing

2021 ◽  
Vol 2 ◽  
Author(s):  
Matthew Dodson ◽  
Annadurai Anandhan ◽  
Donna D. Zhang ◽  
Lalitha Madhavan
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Cell Biology ◽  
Brain Plasticity ◽  
Adult Brain ◽  
Cellular Redox ◽  
Age Related ◽  
Regenerative Activity ◽  
And Function ◽  
Stem Cell State

Redox and metabolic mechanisms lie at the heart of stem cell survival and regenerative activity. NRF2 is a major transcriptional controller of cellular redox and metabolic homeostasis, which has also been implicated in ageing and lifespan regulation. However, NRF2’s role in stem cells and their functioning with age is only just emerging. Here, focusing mainly on neural stem cells, which are core to adult brain plasticity and function, we review recent findings that identify NRF2 as a fundamental player in stem cell biology and ageing. We also discuss NRF2-based molecular programs that may govern stem cell state and function with age, and implications of this for age-related pathologies.

scholarly journals Attributes of Oct4 in stem cell biology: perspectives on cancer stem cells of the ovary

2012 ◽  
Vol 5 (1) ◽  
pp. 37 ◽  
Author(s):  
Chantel Samardzija ◽  
Michael Quinn ◽  
Jock K Findlay ◽  
Nuzhat Ahmed
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Cancer Stem Cells ◽  
Cell Biology ◽  
Stem Cell Biology

T1150 Role of Cyclooxygenase-2 in Cancer Stem Cell Biology and Effect of COX-2 Inhibitors in Anti-Cancer Therapy Targeting Cancer Stem Cells

2010 ◽  
Vol 138 (5) ◽  
pp. S-499
Author(s):  
Masahiko Tsujii ◽  
Jumpei Kondo ◽  
Tomofumi Akasaka ◽  
Ying Jin ◽  
Yoshito Hayashi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Cancer Stem Cells ◽  
Cancer Stem Cell ◽  
Cell Biology ◽  
Stem Cell Biology ◽  
Anti Cancer ◽  
Cox 2 ◽  
Cox 2 Inhibitors

scholarly journals Recent update in identifying Cancer Stem Cells

2018 ◽  
Vol 2 (2) ◽  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Cancer Stem Cells ◽  
Cell Biology ◽  
Primary Source ◽  
Short Review ◽  
Stem Cell Population ◽  
Tumor Initiating Cells ◽  
Proliferating Cells ◽  
Novel Strategy

Last three decades, significant revolutions have been observed in the field of Stem cell biology. Stem cells are quite complex. Several studies revealed their progression and transition of stem cells in various lineages has been effectively characterized in various systems. (CSCs) cancer stem cells remains in a variety of malignancies and they are the primary source of all tumors, which metastasis and relapse of the disease state. Various types of biologically distinct rare populations of “several tumor-initiating” cells have been reported in various cancers. CSCs, are dormant in nature and slow proliferating cells, under certain conditions, which can regenerate into a tumor. The present conventional therapies target only fast multiplying cells within the tumor, which tend to leave the quiescent or slow proliferative cancer stem cell population remains intact, which provides an opportunity to further reinitiate the tumor under favourable conditions. Moreover the current drugs are major failure in eradicating the proliferation of CSCs. Therefore developing new age therapeutics against CSCs is novel strategy, however there are limitations in Identifying CSCs. The Present short review highlights the importance of markers in identifying CSCs different types of cancers.

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