scholarly journals Leishmaniasis Panamensis Masquerading as Myiasis and Sporotrichosis: A Clinical Pitfall

2015 ◽  
Vol 2015 ◽  
pp. 1-3 ◽  
Author(s):  
Peter G. Pavlidakey ◽  
Thy Huynh ◽  
Kristopher Michael McKay ◽  
Naveed Sami
Keyword(s):  
Polymerase Chain Reaction ◽  
Clinical Picture ◽  
Blood Smear ◽  
Peripheral Blood Smear ◽  
Giemsa Stain ◽  
Sodium Stibogluconate ◽  
Chain Reaction ◽  
Upper Arm ◽  
Central Ulceration ◽  
Polymerase Chain

We report a case of cutaneous leishmaniasis panamensis in nonendemic Costa Rica. A 19-year-old female presented with nonhealing, unilateral eruption of erythematous papules with superficial central ulceration in a sporotrichoid pattern on right upper arm and back. Given the clinical picture and geographic locale, the patient was initially diagnosed with myiasis or human botfly infestation; however, the sporotrichoid pattern of the bites is an unlikely finding in myiasis. Peripheral blood smear, Giemsa stain, and polymerase chain reaction (PCR) were consistent forLeishmaniaspp. Ulceration resolved with 20-day course of IV sodium stibogluconate.

Vivax malaria presenting with cerebral malaria and convulsions

2010 ◽  
Vol 55 (1) ◽  
Author(s):  
Anupkumar Anvikar ◽  
Dinesh Singh ◽  
Ruchi Singh ◽  
Aditya Dash ◽  
Neena Valecha
Keyword(s):  
Polymerase Chain Reaction ◽  
Platelet Count ◽  
Vivax Malaria ◽  
Laboratory Tests ◽  
Enteric Fever ◽  
Blood Smear ◽  
Peripheral Blood Smear ◽  
Chain Reaction ◽  
Low Platelet Count ◽  
Polymerase Chain

AbstractA patient was admitted with fever, vomiting, restlessness and convulsions. He was febrile and unconscious. Laboratory tests showed a low platelet count and ruled out enteric fever and dengue. His peripheral blood smear was positive for Plasmodium vivax. The presence of P. vivax monoinfection was confirmed by polymerase chain reaction and DNA sequencing. The report highlights the importance of considering the possibility of complications even in P. vivax malaria and formulation of strategies accordingly.

Acute Granulocytic Ehrlichiosis in a Rottweiler

2005 ◽  
Vol 41 (5) ◽  
pp. 323-326 ◽  
Author(s):  
Wendy G. Arsenault ◽  
Joanne B. Messick
Keyword(s):  
Polymerase Chain Reaction ◽  
Inclusion Bodies ◽  
Blood Smear ◽  
Deoxyribonucleic Acid ◽  
Peripheral Blood Smear ◽  
Chain Reaction ◽  
Granulocytic Ehrlichiosis ◽  
Intracytoplasmic Inclusion ◽  
Intracytoplasmic Inclusion Bodies ◽  
Polymerase Chain

Acute granulocytic ehrlichiosis was identified in a 6-year-old rottweiler that was presented for possible pancreatitis. Intracytoplasmic inclusion bodies were identified within neutrophils on a peripheral blood smear. Serology was ineffective in identifying the disease in the acute state. The diagnosis and identification of the organism were confirmed by polymerase chain reaction (PCR) and deoxyribonucleic acid (DNA) sequencing. Based on elevations in amylase and lipase and the presence of right cranial-quadrant abdominal pain, concurrent pancreatitis was diagnosed. It is unknown if there was any association between the acute granulocytic ehrlichiosis and the pancreatitis. The dog recovered well following doxycycline therapy.

scholarly journals PCR - based diagnosis to evaluate the performance of malaria reference centers

2004 ◽  
Vol 46 (4) ◽  
pp. 183-187 ◽  
Author(s):  
Silvia Maria Di Santi ◽  
Karin Kirchgatter ◽  
Karen Cristina Sant'Anna Brunialti ◽  
Alessandra Mota Oliveira ◽  
Sergio Roberto Santos Ferreira ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Gold Standard ◽  
Blood Smear ◽  
Plasmodium Species ◽  
Thick Blood Smear ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Positive Results ◽  
Ssrrna Gene

Although the Giemsa-stained thick blood smear (GTS) remains the gold standard for the diagnosis of malaria, molecular methods are more sensitive and specific to detect parasites and can be used at reference centers to evaluate the performance of microscopy. The description of the Plasmodium falciparum, P. vivax, P. malariae and P. ovale ssrRNA gene sequences allowed the development of a polymerase chain reaction (PCR) that had been used to differentiate the four species. The objective of this study was to determine Plasmodium species through PCR in 190 positive smears from patients in order to verify the quality of diagnosis at SUCEN's Malaria Laboratory. Considering only the 131 positive results in both techniques, GTS detected 4.6% of mixed and 3.1% of P. malariae infections whereas PCR identified 19.1% and 13.8%, respectively.

scholarly journals Evaluation on the presence of Anaplasma, Ehrlichia, and Babesia spp. in goats (Capra hircus) in Cebu, the Philippines

2019 ◽  
Vol 12 (6) ◽  
pp. 774-777 ◽  
Author(s):  
Adrian P. Ybañez ◽  
Orgil V. Arrabis ◽  
Dennis Justin M. Alvarez ◽  
Eloiza May S. Galon ◽  
Rhea Mae P. Jayag ◽  
...  
Keyword(s):  
Blood Smear ◽  
Peripheral Blood Smear ◽  
The Philippines ◽  
Capra Hircus ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Blood Samples ◽  
Blood Smear Examination ◽  
Polymerase Chain ◽  
Babesia Spp

Background: Tick-borne diseases are caused by a wide variety of viruses, pathogens, and diseases. Anaplasma, Ehrlichia, and Babesia spp. are among the most known tick-borne pathogens in Asia. In the Philippines, these pathogens were already reportedly present in dogs and large ruminants, but no study has been reported yet evaluating their presence in goats. Aim: The present study aimed to evaluate the presence of Anaplasma, Ehrlichia, and Babesia spp. in goats in Cebu, the Philippines. Materials and Methods: A total of 100 blood samples from goats were collected in Cebu, the Philippines. Profile of sampled goats including age, body score, and sex was obtained. Peripheral blood smear examination and DNA extraction were performed. Nested polymerase chain reaction assay was used to evaluate the presence of Anaplasma, Ehrlichia, and Babesia spp. Results: None of the samples were found positive with Anaplasma, Ehrlichia, and Babesia spp. infection. Conclusion: Tested goats were negative with Anaplasma, Ehrlichia, and Babesia spp. and calls for continuous surveillance of these pathogens due to the reported detection of these pathogens in other livestock animals in the area.

A case report of Brugian filariasis outside an endemic area in Thailand

2012 ◽  
Vol 87 (4) ◽  
pp. 510-514 ◽  
Author(s):  
S. Yokmek ◽  
W. Warunyuwong ◽  
S. Rojanapanus ◽  
C. Jiraamornimit ◽  
J.J. Boitano ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Endemic Area ◽  
Blood Smear ◽  
High Resolution Melting ◽  
Polymerase Chain Reaction Analysis ◽  
Domestic Cats ◽  
Thick Blood Smear ◽  
Chain Reaction ◽  
Presenting Symptoms ◽  
Polymerase Chain

AbstractA 2-year-old boy living outside the endemic area of lymphatic filariasis in Surat Thani Province, Thailand, developed a high fever. To investigate the cause of his presenting symptoms, blood was collected and microfilariae were detected and identified as Brugia malayi using thick blood smear staining. The sources of the infection were investigated. Microfilariae from two domestic cats residing in the boy's village were detected and identified as B. pahangi using a high-resolution melting real-time polymerase chain reaction analysis. The possible sources of this cryptic infection are discussed.

scholarly journals Canine ehrlichiosis: prevalence and epidemiology in northeast Brazil

2015 ◽  
Vol 24 (2) ◽  
pp. 115-121 ◽  
Author(s):  
Paula Elisa Brandão Guedes ◽  
Thais Nascimento de Andrade Oliveira ◽  
Fábio Santos Carvalho ◽  
Renata Santiago Alberto Carlos ◽  
George Rego Albuquerque ◽  
...  
Keyword(s):  
Blood Smear ◽  
Peripheral Blood Smear ◽  
Diagnostic Methods ◽  
Chain Reaction ◽  
Factors Associated ◽  
Molecular Tests ◽  
Canine Ehrlichiosis ◽  
Indirect Immunofluorescent Assay ◽  
Polymerase Chain ◽  
Indirect Immunofluorescent

Ehrlichiosis is a zoonotic disease that is caused by bacteria of the genus Ehrlichia. The aims of this study were to detect the presence of Ehrlichia spp. in the blood of dogs in Ituberá, Bahia, and to compare the sensitivities and specificities of blood smear, serological, and molecular examinations. Furthermore, this study identified factors associated with exposure to the agent in dogs in this locality. Blood samples were collected from 379 dogs and submitted for indirect immunofluorescent assay and polymerase chain reaction testing for the detection of Ehrlichia spp. antibodies and DNA, respectively. Additionally, a peripheral blood smear was obtained from the ear tip for parasite identification. Of the 379 animals, 12.4%, 32.7%, and 25.6% were identified as positive on the blood smear, serological, and molecular tests, respectively. The dogs positive in one of the three techniques were considered exposed (46.9%). Younger dogs and rural habitat were protective factors and presence of ticks and contact with other dogs were the risk factors associated with exposure to the agent. It was concluded that dogs of Ituberá have high positivity for Ehrlichia spp. and that the diagnostic methods used for detection are complementary.

scholarly journals PERBANDINGAN DETEKSI Plasmodium spp. ANTARA CARA PEMERIKSAAN MIKROSKOPIK SEDIAAN DARAH TIPIS DENGAN TEKNIK POLYMERASE CHAIN REACTION

2014 ◽  
Vol 2 (1) ◽  
Author(s):  
Rilvia Mona Cambey
Keyword(s):  
Polymerase Chain Reaction ◽  
Human Development ◽  
Microscopic Examination ◽  
Predictive Value ◽  
Gold Standard ◽  
Human Development Index ◽  
Blood Smear ◽  
Thin Blood Smear ◽  
Chain Reaction ◽  
Polymerase Chain

Abstract: Malaria disease is one of the priority of the health programs because it affects the Human Development Index, which result in icreased mortality among infants, toddlers, pregnant woman and adults. WHO noted that in 2010 there was 219 million cases of malaria with mortality rate of 660.0000 annually.Until now, the microscopic method who has many limitation is  still the gold standard in malaria examination. On other side, Polymerase Chain Reaction which proved accurate as it can identify Plasmodium up to the stage of DNA is not yet use as routine examination of Malaria Disease. The purpose of this study was compare the sensitivity and specificity of detection Plasmodium spp. using the gold standard Microscopic examination (thin blood smear)  with the Polymerase Chain Reaction (PCR) method. The design of this study is Diagnostic Test with a sample of 30 people whoose blood was drawn in malaria patients who come to the RSU Budi Mulia Bitung and RS Manembo-nembo since September 2013- Januari 2014. Blood taken made into a thin blood smear  then extracted and proceeded  to PCR. Then from the result of both test, diagnostic test applied to determine the level of sensitivity,spesificity,positif predictive value, and negatif predictive value. Results: The level of sensitivity of the PCR was 100%,specificity 60%, positif predictive value 83,33&, and negatif predictive value 100%.Conclusion: PCR is more accurate in determining the plasmodium species and produce fewer errors than the diagnosis of Microscopic examination thin blood smears. Keywords: Microscopic Examination, Thin blood smear, Polymerase Chain Reaction (PCR), sensitivity, specificity   Abstrak: Malaria merupakan salah satu prioritas program kesehatan karena mempengaruhi Human Development Index yang mengakibatkan meningkatnya angka kematian pada bayi, balita, ibu hamil, dan orang-orang dewasa. WHO mencatat pada tahun 2010 terdapat 219 juta kasus malaria dengan angka kematian 660.000 setiap tahunnya. Sampai sekarang ini, metode mikroskopis yang memiliki banyak keterbatasan masih menjadi standar baku emas dalam pemeriksaan malaria. Dilain pihak Polymerase Chain Reaction yang terbukti akurat karena dapat mengidentifikasi plasmodium sampai pada tahap DNA belum dijadikan pemeriksaan rutin penyakit malaria.Tujuan : Penelitian ini membandingkan tingkat sensitivitas dan spesifisitas deteksi Plasmodium spp menggunakan pemeriksaan baku emas yaitu mikroskopik sediaan darah tipis dengan Polymerase Chain Reaction (PCR). Metode : Penelitian ini merupakan penelitian uji diagnostik dengan sampel sejumlah 30 orang yang darahnya diambil  pada pasien malaria yang datang ke RSU Budi Mulia dan RS Manembo-nembo sejak bulan September 2013 - Januari 2014. Darah yang diambil, dibuat menjadi hapusan darah tipis, kemudian diekstraksi dan di lanjutkan ke pemeriksaan PCR. Kemudian dari hasil kedua pemeriksaan, dilakukan uji diagnostik untuk mengetahui tingkat sensitivitas, spesifisitas, nilai duga positif dan nilai duga negatif. Hasil: Tingkat sensitivitas PCR sebesar 100%, spesifisitas sebesar 60%, nilai duga positif sebesar 83,33% dan nilai duga negatif sebesar 100%.Simpulan: PCR lebih akurat dalam menentukan spesies plasmodium dan lebih sedikit menghasilkan kesalahan diagnosis daripada pemeriksaan mikroskopik sediaan darah tipis.Kata Kunci: Pemeriksaan Mikroskopis, Sediaan Darah Tipis,Polymerase Chain Reaction (PCR), Sensitivitas, Spesifisitas.

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