scholarly journals The Anticandidal and Toxicity Properties of Lampranthus francisci

2015 ◽  
Vol 2015 ◽  
pp. 1-15 ◽  
Author(s):  
Batanai Moyo ◽  
Stanley Mukanganyama
Keyword(s):  
Red Blood Cells ◽  
Peritoneal Cavity ◽  
Blood Cells ◽  
Fungicidal Activity ◽  
Antifungal Agents ◽  
Fungal Infections ◽  
Ethanol Extract ◽  
Aqueous Extracts ◽  
Succulent Plant ◽  
Mouse Cells

Fungal infections have been rising due to the increasing number of immunocompromised patients and intensive use of some antifungal agents. Lampranthus francisci is an ornamental succulent plant. In Zimbabwe, the fresh sap from the leaves is used to treat fungal scalp infections. The activity of L. francisci fresh and dry acetone, ethanol, hydroethanolic, and aqueous extracts against Candida albicans and Candida krusei was determined. Mouse peritoneal cavity cells and sheep red blood cells were used to investigate L. francisci’s toxicity profile. The hydroethanolic extracts were the most effective extracts against C. albicans. The fresh ethanol extract was the most effective extract against C. krusei. The dry acetone extract, dry ethanol extract, and the fresh and dry aqueous extracts promoted the growth of C. krusei. The hydroethanolic extracts caused haemolysis of sheep cells. The hydroethanolic extracts promoted the growth of the mouse peritoneal cavity red blood cells. Both aqueous extracts increased the density of the mouse cells, but only the fresh extract increased the metabolism of the mouse cells. L. francisci has some fungicidal activity and boosts the growth of immune cells, thus validating its use in ethnomedicine. L. francisci extracts are potential leads for the isolation of immune stimulatory compounds.

scholarly journals Black and white teas as potential agents to combine with amphotericin B and protect red blood cells from amphotericin B-mediated toxicity

2018 ◽  
Vol 78 (4) ◽  
pp. 673-678 ◽  
Author(s):  
V. M. Oliveira ◽  
N. M. Khalil ◽  
E. Carraro
Keyword(s):  
Side Effects ◽  
Red Blood Cells ◽  
Amphotericin B ◽  
Blood Cells ◽  
Fungal Infections ◽  
Black Tea ◽  
Additive Effects ◽  
Antifungal Effect ◽  
Black And White ◽  
Fungicidal Substance

Abstract Amphotericin B is a fungicidal substance that is treatment of choice for most systemic fungal infections affecting immunocompromised patients. However, severe side effects have limited the utility of this drug. The aim of this study was to evaluate the antifungal effect of the combination of amphotericin B with black tea or white tea and protective of citotoxic effect. The present study shows that white and black teas have additive effects with amphotericin B against some species Candida. In addition, the combination of white and black tea with amphotericin B may reduce the toxicity of amphotericin B to red blood cells. Our results suggest that white and black tea is a potential agent to combine with amphotericin for antifungal efficacy and to reduce the amphotericin dose to lessen side effects.

scholarly journals On opsonins in relation to red blood-cells

1907 ◽  
Vol 79 (528) ◽  
pp. 1-11
Keyword(s):  
Blood Cell ◽  
Red Blood Cells ◽  
Peritoneal Cavity ◽  
Red Blood Cell ◽  
Blood Cells ◽  
Previous Communication

In a previous communication it was pointed out that opsonins, whose existence in relation to bacteria is generally recognised, are also produced by injecting foreign red blood-cells into the peritoneal cavity. The latter variety of opsonin, which is thermostable, not being destroyed by exposure to 58° C. for 30 minutes, is taken up by red blood-cells of the kind used for injection. Such red blood-cells, after being thus “sensibilised” by opsonin, are ingested by leucocytes. Since it appeared obvious that the occurrence of phagocytosis might, under suitable conditions of experiment, be made use of in order to discover if a serum were capable of sensibilising red blood-cells, that is, if it contained opsonin corresponding to the red blood-cells employed, it was determined to ascertain if, proceeding along the line of investigation thus suggested, a method for the estimation of red blood-cell opsonins could be devised. It was further decided to study the nature of the action of such opsonins upon red blood-cells.

scholarly journals Effectiveness of Sansavieria trifasciata Extract Bracelet in Absorbing Carbon Monoxide (Co) in Cigarette Smoke

Jurnal Biota ◽  
1970 ◽  
Vol 5 (2) ◽  
pp. 96-103
Author(s):  
Gendis Siti Rahmawati ◽  
Resti Nuraeni ◽  
Lu’lu Robiatul Fajrin ◽  
Sistiana Windyariani
Keyword(s):  
Carbon Monoxide ◽  
Red Blood Cells ◽  
Experimental Method ◽  
Blood Vessels ◽  
Cigarette Smoke ◽  
Blood Cells ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Randomized Design ◽  
Completely Randomized Design

Carbon monoxide (CO) contained in cigarette smoke is a very strong poison, if it is smoked by the lungs it will be easier to bind hemoglobin, so that red blood cells will lack oxygen, which can cause damage to blood vessels. with the process of atherosclerosis (narrowing). Sansevieria trifasciata has the ability to absorb poisons such as CO. This study aims to determine the effectiveness of Sansevieria trifasciata leaf extract in absorbing CO contained in cigarette smoke and assessing the amount of CO absorbed by Sansevieria trifasciata leaf extract. The method used is a pure experimental method using a Completely Randomized Design (CRD) consisting of 3 different treatments and 3 repetitions. The results of the 3 treatments showed that P1 (Sanseviera trifasciata wet extract) had the ability to absorb CO an average of 12.67 ppm every 15 minutes, in contrast to P2 (ethanol extract of Sanseviera trifasciata) which absorbs CO. 91.11 ppm every 15 minutes P3 (ethanol extract and CMC leaves from Sansevieria trifasciata) has the ability to absorb CO by 37.89 ppm every 15 minutes. The highest absorption effectiveness was obtained by ethanol extract and bracelets containing ethanol extract with the addition of CMC.

scholarly journals Efficacy of the Aqueous Extracts of Justicia Galeopsis Leaves on the Improvement of Hematological Parameters in Anemic Rats

2020 ◽  
Vol 9 (5) ◽  
pp. 14
Author(s):  
Ahou L. Loukou ◽  
Moussa Gbogbo ◽  
Raissa S. Assi ◽  
Louise Atchibri-Anin ◽  
Kouakou Brou
Keyword(s):  
Body Weight ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Hematological Parameters ◽  
Hemoglobin Level ◽  
Albino Rats ◽  
Aqueous Extracts ◽  
Reference Drug ◽  
Phenylhydrazine Hydrochloride ◽  
Wistar Albino Rats

Antianemc potential of aqueous of Justicia galeopsis leaves was studied using Wistar Albino rats after induction of anemia by phenylhydrazine hydrochloride. Forty rats (20 male and 20 female) subdivided into five groups of eight rats were used. There was a group as control (not anemic) and four other anemic groups which had received by gavage respectively 1 ml/kg of distilled water, 1 ml/kg of body weight of Vitafer (reference drug commonly used to treat anemia), 100 mg/kg of body weight of extract of J. galeopsis leaves cooked during 30 min and 30 mg/kg of body weight of extract of J. galeopsis leaves cooked during 45 min. Hematological parameters (red blood cells, hemoglobin and hematocrit) were analyzed as indices of anemia and the weights of specific organs (liver, spleen and kidney) were evaluated. The results of this investigation had showed that aqueous extract of J. galeopsis leaves cooked improved red blood cells, hemoglobin and hematocrit levels. These extracts were not toxic for liver, spleen and kidney. The administration of 100 mg/kg/day of extract of leaves cooked leaves during 30 min promotes a better recovery rate of the number of red blood cells (94.80 %), hemoglobin level (159.53 %) and hematocrit (117.72 %) than Vitafer and the extract of leaves cooked for 45 min. This is suggestive that aqueous extracts of Justicia galeopsis leaves cooked during 30 min may be exploited during 2 weeks in the treatment of anemia.

scholarly journals Cytotoxic effect of Myrtus communis, Aristolochia longa, and Calycotome spinosa on human erythrocyte cells

2021 ◽  
Vol 9 (2) ◽  
pp. 379-386
Author(s):  
Leila Gadouche ◽  
Azdinia Zidane ◽  
Khayra Zerrouki ◽  
Karima Azouni ◽  
Saadia Bouinoune
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Cytotoxic Effect ◽  
Aqueous Extracts ◽  
Myrtus Communis ◽  
Phytochemical Screening ◽  
Human Red Blood Cells ◽  
Wide Range ◽  
Hemolytic Effect ◽  
Low Cytotoxicity

Introduction. Myrtus communis, Aristolochia longa, and Calycotome spinosa are medicinal plants frequently used in Algeria. Some plants can cause a fragility of the erythrocyte membrane and lead to hemolysis. Therefore, we aimed to study the cytotoxicity of aqueous extracts from the aerial part of these species against red blood cells. Study objects and methods. The hemolytic effect was determined spectrophotometrically by incubating an erythrocyte solution with different concentrations of the aqueous extracts (25, 50, 100, and 200 mg/mL) at 37°C during one hour. In addition, we performed phytochemical screening and measured the contents of polyphenols and flavonoids. Results and discussion. After one hour of incubation of human red blood cells with the aqueous extracts at different concentrations, the hemolysis percentage showed a significant leak of hemoglobin with A. longa (68.75 ± 6.11%; 200 mg/mL), the most toxic extract followed by C. spinosa (34.86 ± 5.06%; 200 mg/mL). In contrast, M. communis showed very low cytotoxicity (20.13 ± 3.11%; 200 mg/mL). Conclusion. These plants are sources of a wide range of bioactive compounds but their use in traditional medicine must be adapted to avoid any toxic effect.

Schistosoma mansoni: host antigen occurrence on worms recovered from laboratory vertebrate animals

Parasitology ◽  
1980 ◽  
Vol 81 (2) ◽  
pp. 349-354 ◽  
Author(s):  
P. M. Z. Coelho ◽  
G. Gazzinelli ◽  
J. Pellegrino
Keyword(s):  
Schistosoma Mansoni ◽  
Red Blood Cells ◽  
Peritoneal Cavity ◽  
Convenient Method ◽  
Blood Cells ◽  
Mastomys Natalensis

SUMMARYBy transferring Schistosoma mansoni (12 to 14-day-old worms) from one host into the peritoneal cavity of a host of a different species, it was possible to demonstrate that worms grown in rats, hamsters, Mastomys natalensis and Cebus monkeys were rejected by recipient animals previously immunized against red blood cells of the donor hosts. These results suggest that host-like antigens are acquired by S. mansoni in all hosts. The transfer of worms into the peritoneal cavity has proved to be a simple and convenient method for studying the presence of host antigens in S. mansoni.

scholarly journals Evaluation of Polyphenol Content and Antioxidant Capacity of Aqueous Extracts from Eight Medicinal Plants from Reunion Island: Protection against Oxidative Stress in Red Blood Cells and Preadipocytes

Antioxidants ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 959
Author(s):  
Eloïse Checkouri ◽  
Franck Reignier ◽  
Christine Robert-Da Silva ◽  
Olivier Meilhac
Keyword(s):  
Oxidative Stress ◽  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Medicinal Plants ◽  
Antioxidant Capacity ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Aqueous Extracts ◽  
Polyphenol Content ◽  
Réunion Island

Background—Medicinal plants are traditionally used as infusions or decoctions for their antioxidant, anti-inflammatory, hypolipidemic and anti-diabetic properties. Purpose—The aim of the study was to define the polyphenol composition and to assess the antioxidant capacity of eight medicinal plants from Reunion Island referred to in the French Pharmacopeia, namely Aphloia theiformis, Ayapana triplinervis, Dodonaea viscosa, Hubertia ambavilla, Hypericum lanceolatum, Pelargonium x graveolens, Psiloxylon mauritianum and Syzygium cumini. Methods—Polyphenol content was assessed by biochemical assay and liquid chromatography coupled to mass spectrometry. Antioxidant capacity was assessed by measuring DPPH reduction and studying the protective effects of herbal preparation on red blood cells or preadipocytes exposed to oxidative stress. Results—Polyphenol content ranged from 25 to 143 mg gallic acid equivalent (GAE)/L for infusions and 35 to 205 mg GAE/L for decoctions. Liquid chromatography coupled to mass spectrometry analysis showed the presence of major bioactive polyphenols, such as quercetin, chlorogenic acid, procyanidin and mangiferin. Antioxidant capacity assessed by different tests, including DPPH and Human red blood cell (RBC) hemolysis of herbal preparations, demonstrated a dose-dependent effect whatever the extraction procedure. Our data suggest that decoction slightly improved polyphenol extraction as well as antioxidant capacity relative to the infusion mode of extraction (DPPH test). However, infusions displayed a better protective effect against oxidative stress-induced RBC hemolysis. Conclusion—Traditional preparations of medicinal plant aqueous extracts (infusions and decoctions) display antioxidant properties that limit oxidative stress in preadipocytes and red blood cells, supporting their use in the context of metabolic disease prevention and treatment.

Sign in / Sign up

Export Citation Format

Share Document