scholarly journals Immunohistochemical Expression of Collagens in the Skin of Horses Treated with Leukocyte-Poor Platelet-Rich Plasma

2015 ◽  
Vol 2015 ◽  
pp. 1-12 ◽  
Author(s):  
Maria Verônica de Souza ◽  
Mariana Brettas Silva ◽  
José de Oliveira Pinto ◽  
Marianna Barros de Souza Lima ◽  
Júlio Crepaldi ◽  
...  
Keyword(s):  
Platelet Rich Plasma ◽  
Normal Skin ◽  
Healing Process ◽  
Treated Group ◽  
Type I ◽  
Immunohistochemical Expression ◽  
Reticular Dermis ◽  
Wound Induction ◽  
Three Samples ◽  
The Right

This study evaluated the immunohistochemical expression of type I (COL I) and III (COL III) collagens during the healing process of skin treated with leukocyte-poor platelet-rich plasma (LP-PRP). Seven healthy gelding crossbred horses aged 16 to 17 years were used. Two rectangle-shaped wounds were created surgically in the right and left gluteal regions. Twelve hours after wound induction, 0.5 mL of the LP-PRP was administered in each edge of the wounds of one of the gluteal regions. The contralateral region was used as control (CG). Three samples were obtained: after wound induction (T0), 14 days (T1) of healing process, and after complete closure of the skin (T2). The normal skin (T0) showed strong staining for type III and I collagen in papillary and reticular dermis, respectively. In the scar of the treated group, COL III showed important (p<0.05) increase in immunoreaction in T2 compared with T1. The administration of a single dose of LP-PRP 12 h after induction of wound in horses does not influence formation of collagens I and III. However, the intense labeling for COL III suggests that the tissue was still weak during the macroscopic closure of the wound, demonstrating that healing was not completely finished.

scholarly journals Evaluation of subcutaneous infiltration of autologous platelet-rich plasma on skin-wound healing in dogs

2017 ◽  
Vol 37 (2) ◽  
Author(s):  
Haithem A. Farghali ◽  
Naglaa A. AbdElKader ◽  
Marwa S. Khattab ◽  
Huda O. AbuBakr
Keyword(s):  
Catalase Activity ◽  
Platelet Rich Plasma ◽  
Healing Process ◽  
Scar Tissue ◽  
Treated Group ◽  
Collagen Type I ◽  
Skin Wound ◽  
Type I ◽  
Skin Wound Healing ◽  
Wound Fluid

Platelet-rich plasma (PRP) is known to be rich in growth factors and cytokines, which are crucial to the healing process. This study investigate the effect of subcutaneous (S/C) infiltration of autologous PRP at the wound boundaries on wound epithelization and contraction. Five adult male mongrel dogs were used. Bilateral acute full thickness skin wounds (3 cm diameter) were created on the thorax symmetrically. Right side wounds were subcutaneously infiltrated with activated PRP at day 0 and then every week for three consecutive weeks. The left wound was left as control. Wound contraction and epithelization were clinically evaluated. Expression of collagen type I (COLI) A2, (COLIA2),histopathology and immunohistochemical (IHC) staining of COLI α1 (COLIA1) were performed on skin biopsies at first, second and third weeks. The catalase activity, malondialdehyde (MDA) concentration and matrix metalloproteinase (MMP) 9 (MMP-9) activity were assessed in wound fluid samples. All data were analysed statistically. The epithelization percent significantly increased in the PRP-treated wound at week 3. Collagen was well organized in the PRP-treated wounds compared with control wounds at week 3. The COLIA2 expression and intensity of COLIA1 significantly increased in PRP-treated wounds. MDA concentration was significantly decreased in PRP-treated wound at week 3. The catalase activity exhibited no difference between PRP treated and untreated wounds. The activity of MMP-9 reached its peak at the second week and was significantly high in the PRP-treated group. S/C infiltration of autologous PRP at the wound margins enhances the wound epithelization and reduces the scar tissue formation.

Wound-healing effects of human dermal components with gelatin dressing

2017 ◽  
Vol 32 (6) ◽  
pp. 716-724 ◽  
Author(s):  
Hyun-Jun Jang ◽  
Yu-mi Kim ◽  
Bo-Young Yoo ◽  
Young-Kwon Seo
Keyword(s):  
Wound Healing ◽  
Normal Skin ◽  
Treated Group ◽  
Collagen Type I ◽  
Skin Wound ◽  
Blue Staining ◽  
Elastic Fibers ◽  
Type I ◽  
Initial Area

There have been numerous investigations regarding various types of dressings and artificial dermis of solid form, yet limited research and development on paste types, such as hydrogels with dermal powder, have ensued. In this study, we compared the in vivo wound healing effects of gelatin paste containing dermal powder to a collagen type I/chondroitin 6-sulfate (coll/chondroitin) sponge and gelatin alone, after 48 days post grafting, in a skin wound rat model. In the dermis powder/gelatin paste-treated group, wound area contraction was minimized 50%, while in the gelatin and coll/chondroitin sponge groups, the initial area contracted 83–85% and 79–85%, respectively. Histological analysis revealed the wounds treated with dermal powder/gelatin were associated with many fibroblasts, which infiltrated the wound bed, as well as thick collagen bundles that were arranged in dendritic arrays, resembling normal skin. Furthermore, in contrast to the gelatin- and coll/chondroitin sponge-treated groups, the powder/gelatin paste-treated wounds exhibited an abundance of elastic fibers (Victoria blue staining) and extensive formation of blood vessels around the dermis (CD31 staining). Therefore, the dermis powder/gelatin paste not only renders convenience to users but also has prominent wound-healing effects on full-thickness wounds.

Effects of platelet rich plasma on the gastric serosal surface neomucosa formation: an experimental rodent model

2018 ◽  
Vol 44 (1) ◽  
pp. 32-40
Author(s):  
Binboğa Sinan ◽  
Kasapoğlu Pınar ◽  
Binboğa Elif ◽  
Cikot Murat ◽  
Baytekin Fırat ◽  
...  
Keyword(s):  
Growth Factor ◽  
Transforming Growth Factor ◽  
Platelet Rich Plasma ◽  
Healing Process ◽  
Transforming Growth Factor Β ◽  
Treated Group ◽  
En Bloc Resection ◽  
Bloc Resection ◽  
En Bloc ◽  
Platelet Concentration

Abstract Background Autologous platelet rich plasma (PRP) is the platelet concentration obtained from thrombocytes in the plasma. During the healing process, the platelets are activated and then release the granules which stimulate the inflammatory cascade and healing process. Platelet derived growth factor, vascular endothelial growth factor (VEGF), transforming growth factor β (TGFβ), epidermal growth factor (EGF) and fibroblast growth factor (FGF) are valuable markers used for cell regeneration. The aim of this study was to investigate the potential effects of PRP treatment on the neomucosa formation, a potential technique for increasing the intestinal surface area in patients with short bowel syndrome (SBS). Materials and methods Thirty-two male Wistar-Hannover rats were divided into: sham, control, PRP-treated and last group for PRP preparation (n=8). Plasma levels of VEGF, TGFβ, EGF and FGF were quantified by ELISA. En-bloc resection of anastomotic part was performed and stained with hematoxylin-eosin. Results VEGF, FGF, TGFβ and EGF levels were found significantly increased in PRP-treated group compared to others (p<0.001). Neomucosa formation was observed in experimental groups but the area increased significantly in PRP group, compared to other groups (p<0.001). Conclusion PRP therapy in gastrointestinal anastomoses is truly beneficial and surgically applicable treatment in SBS patients.

Bioartificial bone grafting:Tarsal joint fusion in a dog using a bioartificial composite bone graft consisting of β-tricalciumphosphate and platelet rich plasma – A case report

2005 ◽  
Vol 18 (01) ◽  
pp. 52-54 ◽  
Author(s):  
H.-A. Merten ◽  
A. Bader ◽  
G. Uhr ◽  
A. Deivick ◽  
A. Meyer-Lindenberg ◽  
...  
Keyword(s):  
Bone Graft ◽  
Platelet Rich Plasma ◽  
Healing Process ◽  
Tumor Resection ◽  
Experimental Studies ◽  
Tarsal Joint ◽  
Intertarsal Joint ◽  
The Right ◽  
Large Bone ◽  
Large Bone Defects

SummaryGrafting of large bone defects caused by trauma or tumor resection still remains a problem to solve. In experimental studies as well as in human dentistry, osteoconduction and biodegradation of the β-tricalciumphosphate Cerasorb® as well as osteoinductive capabilities of platelet rich plasma have been proven. In case of luxation of the right tarsal joint, including a compressive fracture of the forth tarsal bone, the recommended use of autologous cancellous bone to support osseous fusion was replaced by the use of a bioartificial bone graft. Biodegradation of the bone graft was proven clinically and radiographically. Complete osseous fusion of the intertarsal joint occurred. The use of the β-tricalciumphosphate Cerasorb in combination with platelet rich plasma to support a partial arthrodesis of the tarsal joint in a dog did not impede the healing process but led to full recovery of the patient, indicating that this concept of bioartificial bone grafting could support bone healing.

scholarly journals EFEK SERBUK KOPI ROBUSTA (Coffea canephora) TERHADAP PENYEMBUHAN LUKA INSISI PADA KELINCI (Oryctolagus cuniculus)

2015 ◽  
Vol 3 (3) ◽  
Author(s):  
Lilian N. Artho ◽  
Jane Wuisan ◽  
J. A. Najoan
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Treated Group ◽  
Coffea Canephora ◽  
Control Group ◽  
Wound Healing Process ◽  
Robusta Coffee ◽  
Coffee Powder ◽  
The Right ◽  
Sterile Gauze

Abstract: Coffee is known as food and drink as well as alternative medicine for various types of wounds. One of the most coffee plants in Indonesia is Robusta coffee (Coffea canephora). This study aimed to determine whether Robusta coffee powder could accelerate the wound healing process. This was an experimental study using four rabbits as subjects. Incisions were made on the rabbit backs, right and left, with a length of 5 cm. Wounds on the left backs, the untreated/control group, were only covered with sterile gauze. Wounds on the right backs, the treated group, were applied with robusta coffee powder and covered with sterile gauze. Robusta coffee powder and gauze were replaced two times a day. Wounds were macroscopically observed for 2 weeks. The results showed that at day 7, the wounds were still equally opened, dry, looked not too deep, and there was a crust on each wound surface. The lengths of the treated wounds were shorter than of the control wounds. At day 14, the edges of the treated wounds looked closer, the crusts were still attached to the wound surfaces and were smaller than of the control wounds. Conclusion: Robusta coffee powder (Coffea canephora) could accelerate the healing process of incised wounds on rabbit skin.Keywords: wound healing, wound incision, robusta coffee powder, coffea canephoraAbstrak: Kopi bukan hanya dikenal sebagai makanan dan minuman saja tetapi bisa juga sebagai obat alternatif dalam menangani berbagai jenis luka. Salah satu jenis tanaman kopi yang paling banyak di Indonesia ialah kopi Robusta (Coffea canephora). Penelitian ini bertujuan untuk menentukan apakah sebuk kopi robusta mempunyai efek untuk mempercepat proses penyembuhan luka. Penelitian ini menggunakan metode eksperimental dengan 4 ekor kelinci sebagai hewan coba. Luka insisi dibuat pada punggung kanan dan kiri kelinci dengan panjang 5 cm. Luka pada punggung kiri merupakan luka kontrol yang tidak diberi perlakuan, hanya ditutupi dengan kasa steril. Luka pada punggung kanan merupakan luka perlakuan dengan diberi serbuk kopi robusta lalu ditutup kasa steril. Serbuk kopi robusta dan kasa diganti dua kali sehari. Pengamatan dilakukan selama 2 minggu dengan membandingkan gambaran makroskopik kedua luka. Hasil penelitian memperlihatkan pada hari ke 7 kedua luka masih sama-sama terbuka, kering, tampak sudah tidak terlalu dalam, dan terdapat kerak yang menempel pada permukaan luka. Ukuran luka perlakuan lebih pendek dari luka kontrol. Hari ke 14 kedua luka sudah mulai menutup, kerak masih menempel pada permukaan luka dan ukuran kerak luka perlakuan lebih kecil dari luka kontrol. Simpulan: Serbuk kopi robusta (Coffea canephora) memiliki efek untuk mempercepat penyembuhan luka insisi pada kulit kelinci.Kata kunci: penyembuhan luka, luka insisi, kopi robusta, coffea canephora

scholarly journals 121 Non-invasive monitoring of hot-iron brands on growing cattle

2019 ◽  
Vol 97 (Supplement_1) ◽  
pp. 49-50
Author(s):  
Douglas R Tolleson ◽  
Erika Campbell ◽  
Nick Garza ◽  
Robert Moen
Keyword(s):  
Near Infrared ◽  
Bos Taurus ◽  
Normal Skin ◽  
Healing Process ◽  
Type Iii Collagen ◽  
Type I ◽  
Invasive Monitoring ◽  
Near Infrared Reflectance ◽  
Linear Discriminant ◽  
Non Invasive

Abstract Hot-iron branding is a traditional form of permanent cattle identification that produces a scar. There is a need for science-based determination of cattle brand age through non-invasive monitoring of the healing process. Near infrared reflectance spectroscopy (NIRS) has been used by medical forensic scientists to obtain such information. Healing of cutaneous injury involves inflammatory (1 to 3 d), proliferative (4 to 21 d), and remodeling (22 to 365 d) phases. Collagen changes as burn scars mature, i.e. there is an increase in the Type I/ Type III collagen ratio compared to normal skin. The altered ratio is evident in a transformation of collagen from a basketweave arrangement to small parallel bundles. During the remodeling phase, due largely to the Type I/III collagen ratio, scar tissue becomes visibly different than un-injured skin. Previous research has examined the differences in hot-iron brands applied to nursing (~30 d old) Bos taurus cross calves at 0, 33, and 153 d post-branding. Our objective was to continue this research by obtaining near infrared spectra (11001700nm) on hot-iron brands applied to 15 weaned (~ 270 d old; 238 ± 7kg) Bos taurus cross steers at 21, 32, and 51 d post-branding. Spectra were obtained on branded (n = 3) and unbranded (n = 3) skin tissue from each animal, each date. Hair was clipped to < 5mm on unbranded skin. Spectra were analyzed as log 1/reflectance with 1st derivative and scatter correction applied. Linear discriminant analysis and regression procedures were applied to distinguish between brand treatment and date post-branding. Spectra from branded and unbranded skin were correctly (P < 0.05) identified at ~95%. Brand age was predicted successfully (P < 0.05) but not accurately enough for forensic application (RSQ = 0.50; RMSE = 9.75 d). The NIRS technique can discriminate differences in the age of cattle brand scars but numerical prediction requires further investigation.

scholarly journals Benefit of Leukocyte- and Platelet-Rich Plasma in Operative Wound Closure in Oral and Maxillofacial Surgery

2016 ◽  
Vol 2016 ◽  
pp. 1-5 ◽  
Author(s):  
Agata Cieślik-Bielecka ◽  
Justyna Glik ◽  
Rafał Skowroński ◽  
Tomasz Bielecki
Keyword(s):  
Oral Mucosa ◽  
Platelet Rich Plasma ◽  
Healing Process ◽  
Maxillofacial Surgery ◽  
Mandibular Fracture ◽  
Control Group ◽  
Oral And Maxillofacial Surgery ◽  
Mandibular Fractures ◽  
The Right ◽  
Healing Processes

This article reports the influence of an autologous leukocyte- and platelet-rich plasma (L-PRP) injection as a minimally invasive method on supporting wound healing processes after a mandibular odontogenic cystectomy and double mandibular fracture fixation. 113 patients were enrolled into a control group (received no L-PRP injection) and 102 patients were enrolled into an L-PRP group with an oral mucosa incision. 18 patients after a double mandibular fracture were operated on using 2 external submandibular approaches receiving no fluids in the right site (a control group) and an L-PRP injection in the left incision (L-PRP group). Clinical observations showed that the oral mucosa healed faster in patients treated with L-PRP, in comparison to cases where inductive biomaterial was not added. Pain at the L-PRP injection site was relieved within few hours after an operation in patients with double mandibular fractures. However, there were no differences observed in the progression of the healing process. L-PRP possesses inductive properties that could stimulate healing processes and it seems to be one of the most promising methods in the future for the treatment of soft tissue defects.

scholarly journals Co-treatment of wounds in rabbit skin with equine platelet-rich plasma and a commercial ointment accelerates healing

2020 ◽  
Vol 21 ◽  
Author(s):  
Rodrigo Supranzetti de Rezende ◽  
Duvaldo Eurides ◽  
Endrigo Gabelline Leonel Alves ◽  
Guilherme Costa Venturini ◽  
Rodrigo Lopes de Felipe
Keyword(s):  
Platelet Rich Plasma ◽  
Healing Process ◽  
Histological Evaluation ◽  
Group Iii ◽  
Wound Induction ◽  
Group I ◽  
Microscopic Evaluation ◽  
Autologous Platelet Rich Plasma ◽  
Skin Removal ◽  
Treatment Of Wounds

Abstract Autologous platelet-rich plasma (PRP) is beneficial in the healing process of reconstructive surgeries, contributing to the stimulation of angiogenesis; however, heterologous plasma has been shown to be more effective. The objective of this study was to verify, by macro- and microscopic evaluation, whether PRP accelerates the healing process as compared to a commercial ointment containing gentamicin, sulfanilamide, sulfadiazine, urea, and vitamin A (study ointment). This study used 24 white New Zealand rabbits, aged 12 months, and each weighing approximately 3 kg. The animals were separated into 4 equal groups and underwent wound induction through skin removal in the thoracic (study wound) and lumbar (control wound) dorsal midline. Study wounds were co-treated with equine PRP and the study ointment. Control wounds were treated with only the study ointment. Group I underwent histological evaluation 3 days after the skin removal procedure, group II after 7 days, group III after 14 days, and group IV after 21 days. A skin fragment was collected from each animal for histological evaluation. The co-treatment with heterologous equine PRP and the study ointment accelerated the healing process in the surgically induced skin wounds, confirming the acceleratory effect of PRP on wound healing.

scholarly journals Effect of Lyophilized Growth Factors (LGF) Derived from Equine Platelets on Experimentally Induced Skin Wound Healing in Mongrel Dogs

2021 ◽  
Vol 10 (2) ◽  
pp. 75-82
Keyword(s):  
Growth Factors ◽  
Growth Factor ◽  
Healing Process ◽  
Treated Group ◽  
Skin Wound ◽  
Skin Wounds ◽  
Skin Wound Healing ◽  
Thickness Skin ◽  
The Right ◽  
Tgf Β1

Lyophilized growth factor (LGF) is a novel advanced platelets rich protein growth factor. It has been successfully applied in various fields of regenerative medicine including management of chronic and non-healing wounds and ulcers. Our study aimed to evaluate the effect of subcutaneous infiltration of equine platelets derived lyophilized growth factors on healing of induced full thickness skin wound in dogs. Four healthy mongrel dogs of different ages were used in the current work. Three bilateral critical sized skin wounds were done on the back of each dog. After 24 hours, the right-side wounds were injected in 4 cardinal points around each wound by Saline/lidocaine (control wounds) and simultaneously reconstituted LGF was subcutaneously injected around the left side wounds (LGF treated wounds). Wound contraction was monitored physically and histopathologically. The expression of TGF-β1 and NF-κB was evaluated in wound specimens of both groups. A significant reduction in wound size was recorded in LGF treated group compared to saline treated group. The histopathological scoring of the healing progress revealed significant increase in the degree of re-epithelization bridging the wound edges and collagen deposition in LGF-treated wounds compared to control non-treated wounds along the experimental periods. Additionally, the expression of TGF-β1 and NF-κB showed significant elevation in LGF-treated wounds compared with their expression in control wounds. In conclusion, LGF therapy could be a superior candidate as a regenerative therapy in skin wounds that can positively impact healing process of the cutaneous wounds.

scholarly journals Autologous platelet-rich plasma action on skin autografts in horses

2021 ◽  
Vol 51 (8) ◽  
Author(s):  
Ana Carolina Barros da Rosa Pedroso ◽  
Ana Kellen Lima de Queiroz ◽  
Eric Saymon Andrade de Brito ◽  
Joel Phillipe Costa e Souza ◽  
Marina Pacheco Miguel ◽  
...  
Keyword(s):  
Platelet Rich Plasma ◽  
Gluteal Region ◽  
Cutaneous Wounds ◽  
Wound Induction ◽  
Skin Repair ◽  
Autologous Platelet Rich Plasma ◽  
Receive Treatment ◽  
The Right ◽  
Autologous Platelet ◽  
Centrifugation Protocol

ABSTRACT: This study evaluated the action of autologous platelet-rich plasma (PRP) on cutaneous wounds, containing skin autografts, in the gluteal region of horses. Seven healthy horses were used. Two 6 x 6cm cutaneous wounds were produced on each side of the gluteal region. Eight days after wound induction, grafts were performed with skin fragments harvested from the neck, as well as the application of PRP, prepared by double-centrifugation protocol. Wounds with autografts on the left side received PRP (group T), and those with autografts on the right side did not receive treatment (group C). Macroscopic and microscopic evaluations were performed, considering the integration of autografts and retraction of wound edges, as well as neovascularization, inflammatory infiltrate, young fibroblasts, collagenization, reepithelization and autografts integration. There was no difference between the groups (P > 0.05) in relation to most macroscopic and microscopic variables. However, neovascularization was significantly greater (p = 0.0191) in group T, on the 14th day after grafting. It is concluded that PRP favors the process of skin repair with autografts in horses, since it increases the neovascularization in the initial phase of wound healing. Furthermore, the PRP seems to positively influence the integration of the skin autografts and the retraction of the wound edges.

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