scholarly journals Blood Vessel Matrix Seeded with Cells: A Better Alternative for Abdominal Wall Reconstruction—A Long-Term Study

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Maciej Nowacki ◽  
Arkadiusz Jundziłł ◽  
Łukasz Nazarewski ◽  
Andrzej Kotela ◽  
Tomasz Kloskowski ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Abdominal Wall ◽  
Abdominal Wall Defect ◽  
Abdominal Wall Reconstruction ◽  
Assessment Method ◽  
Group Iii ◽  
Long Term Study ◽  
Group I ◽  
Triton X ◽  
And Control

Purpose. The aim of this study was to present abdominal wall reconstruction using a porcine vascular graft seeded with MSC (mesenchymal stem cells) on rat model.Material and Methods. Abdominal wall defect was prepared in 21 Wistar rats. Acellular porcine-vascular grafts taken from aorta and prepared with Triton X were used. 14 aortic grafts were implanted in place, of which 7 grafts were seeded with rat MSC cells (Group I), and 7 were acellular grafts (Group II). As a control, 7 standard polypropylene meshes were used for defect augmentation (Group III). The assessment method was performed by HE and CD31 staining after 6 months. The mechanical properties have been investigated by Zwick&Roell Z0.5.Results. The strongest angiogenesis and lowest inflammatory response were observed in Group I. Average capillaries density was 2.75, 0.75, and 1.53 and inflammatory effect was 0.29, 1.39, and 2.72 for Groups I, II, and III, respectively. The means of mechanical properties were12.74±1.48,7.27±1.56, and14.4±3.7 N/cm in Groups I and II and control, respectively.Conclusions. Cell-seeded grafts have better mechanical properties than acellular grafts but worse than polypropylene mesh. Cells improved mechanical and physiological properties of decellularized natural scaffolds.

Efficacy of Ovsynch and Ovsynch Plus Protocol for Improvement of Fertility in Postpartum Sahiwal Cows

2019 ◽  
Vol 14 (04) ◽  
Author(s):  
J. P. Lakher ◽  
M. K. Awasthi ◽  
J. R. Khan ◽  
M. R. Poyam
Keyword(s):  
Fixed Time ◽  
Conception Rate ◽  
Control Group ◽  
Group Iii ◽  
Treatment Groups ◽  
Group I ◽  
Early Postpartum ◽  
The Status ◽  
Sahiwal Cows ◽  
And Control

The present study was conducted to investigate the efficacy of Ovsynch and Ovsynch plus protocol in postpartum (day 60) Sahiwal cows (n=18). Animals were randomly divided into three equal groups, viz., Ovsynch group Ovsynch plus group and Control group. Animals of group I (n = 6) were treated with traditional Ovsynch protocol. The animals (n = 6) of group II were treated with Ovsynch plus protocol which consisted of an initial intramuscular injection of eCG (Folligon) @ 250 IU on day 60 postpartum followed 3 days later by GPG (Ovsynch) protocol. In group-III Control, no treatment was given to animals (n = 6). Treated animals were inseminated at a fixed time between 14 and 20 hrs after second GnRH injection, irrespective of estrus detection. Blood samples were collected from each animal on days 50 and 60 postpartum to determine the status of cyclicity in animals based on serum concentrations of progesterone (P4). A third blood sample was collected on the day of prostaglandin treatment to determine the response of first GnRH injection. Four animals each were cyclic, and two were acyclic in both treatment groups. Four animals each responded to first GnRH treatment in both treatment groups. Similarly, two animals each got conceived giving conception rate of 50% (2/4) in each treatment. In the control group, one out of 6 animals got conceived yielding 16.66 % conception rate (1/6) during the study period. It may be thus concluded that Ovsynch and Ovsynch plus protocol may be used during the early postpartum period to improve the reproductive efficiency in postpartum Sahiwal cows.

Serum Endocrine Profile in Relation to Estrus Synchronization in Surti Does

2018 ◽  
Vol 13 (03) ◽  
Author(s):  
M. M. Chaudhary ◽  
C. T. Khasatiya ◽  
S. B. Patel ◽  
S. S. Chaudhary ◽  
V. B. Atara ◽  
...  
Keyword(s):  
Jugular Vein ◽  
Estrus Synchronization ◽  
Progesterone Concentration ◽  
Serum Progesterone ◽  
Blood Samples ◽  
Group Iii ◽  
Group I ◽  
Endocrine Profile ◽  
Group Ii ◽  
And Control

The serum progesterone and estradiol profiles during synchronization of estrus by buck effect and PGF2α treatments were monitored in Surti does. Total eighteen non-pregnant does selected were evenly divided into 3 groups, 6 does in each group. The does of Group I were teased with a sexuallyactive- apronized buck; and those of Group II were treated with PGF2α, i.e., Inj. Lutalyse® @ 7.5 mg/doe IM twice 11 days apart, while the Group III served as untreated control. Blood samples were collected from all the animals on day 0 (before 1st PGF2α injection), 3rd day (during treatment), 11th day (before 2nd PGF2α injection), 14th day (after treatment) and 40th day (post-service) by jugular vein puncture. The serum separated was stored at -20°C till further analysis. In all the three groups, 83.33% does, conceived at first service in the sampling cycle. The overall mean serum progesterone concentration of Group I does (5.82±0.72 ng/ml) was significantly higher (p less than 0.01) as compared to Group II (2.93±0.38 ng/ml) and III (2.88±0.30 ng/ml). Similarly, the overall mean serum progesterone concentration of Surti does on day 0 (2.65±0.46 ng/ml), 3rd (2.56±0.80 ng/ml), 11th (4.45±0.84 ng/ml) and 14th (3.40±0.63 ng/ml) did not differ significantly, but the overall mean level at day 40 (6.31±0.45 ng/ml) was significantly (p less than 0.01) higher, because most of animals became pregnant at that time. The overall mean serum oestradiol-17β levels of Group I (24.40±2.98 pg/ ml) was significantly higher (p less than 0.01) than in Group II (15.77±1.77 pg/ml) and III (12.21±1.45 pg/ ml). On the other hand, the overall mean serum oestradiol-17β levels of Surti does on day 0 (12.89±1.21 pg/ml), 3rd (15.84±1.74 pg/ml), 11th (14.81±1.96 pg/ml), 14th (22.15±2.97 pg/ml) and 40th (21.64±5.16 pg/ml) did not differ significantly (p>0.05) and the slightly higher overall mean level found at 40th day might be the influence of the non-pregnant does at first service in the cumulative animals. The hormonal profile reflected the initiation of cyclicity and establishment of pregnancy in treated and control animals.

scholarly journals Effect of Fiber Rich Product on Childhood Obesity and Lipid Profile Aged 10-12 Years

2021 ◽  
Vol 9 (3) ◽  
pp. 87-90
Author(s):  
A. Jyoth ◽  
Keyword(s):  
Exercise Group ◽  
Control Group ◽  
Group Iii ◽  
Group I ◽  
Diet Survey ◽  
Group Ii ◽  
And Control ◽  
Experimental Group ◽  
Positive Results ◽  
Green Peas

The effect of fiber rich product on child hood obesity studied with 60 sample which were collected randomly among 10-12 years and categorized into 2 groups i.e, experimental group and control group. Experimental group further categorized into three groups i.e, supplementation with exercise (n=15), only supplementation group (n=15), only exercise group (n=15) and control group consists of (n=15).Anthropometric, and diet survey conducted as parameters. A fiber rich product was prepared and supplemented for 2 months to the selected subjects and it consists of whole Bengal gram, whole green gram, green peas, barley and jaggery. Positive results were obtained in three experimental groups. Significant decrease observed inweight, and BMI, Total cholesterol, Triglycerides, LDL, VLDL significant increase observed in HDL in group I II and III. The results were (51.60-48.26kg, 24.7-23.1, 195.2-152.3 mg/dl, 168.2-145.0 mg/dl, 52.2-54.13 mg/dl, 109.4- 69.4mg/dl, 33.6-28.7mg/dl) in group I, In group II the results are (50.3-49.86kg, 23.7-23.4, 168.6-161.0mg/dl, 145.4-129.6mg/dl, 44.2-45.2 mg/dl, 95.3-90.0mg/dl, 29.1-28.3mg/dl).In group III the tesults aere (50.7-49.6kg, 24.5-23.9, 143.2-139.3mg/dl, 139-134.5mg/dl, 38.2-38.7mg/dl 76.8-74.1 mg/dl, 25.6-28.1mg/dl) .From the results it was clear that when compared to group II and III group I has shown better results.

scholarly journals Anti-allergic Activity of 70% Ethanol Extract of Strawberries in Ovalbumin Induced Male Mice

2020 ◽  
Vol 5 (3) ◽  
pp. 92-97
Author(s):  
Siska ◽  
Diene Roufiani ◽  
Ema Dewanti
Keyword(s):  
Ethanol Extract ◽  
Control Group ◽  
Fruit Extract ◽  
Antiallergic Activity ◽  
Group Iii ◽  
Skin Contact ◽  
Group I ◽  
And Control ◽  
Control Group Group

Anaphylaxis is the most common allergic reaction triggered by allergens such as insect poisons, food, and drugs through skin contact, injection, or inhalation. In vitro previous research showed that strawberries fruit have activity as antioxidant, anticancer, anti-inflammation, and anti-allergic. The research aimed to determine the antianaphylaxis strawberry fruit extract in mice (Balb/C strain) with ovalbumin-induced.  Twenty-four Balb/C strain mice were divided into six groups (n=4). Group I and II as a normal and control group. Group III till VI as a treatment group was given cetirizine dose 0.042 mg/20 g BW and strawberry extract doses 0,68; 1,36; and 2,72 mg/20 g BW, respectively. This research showed that 70 % of ethanol extract of strawberries fruit have antiallergic activity in response to active cutaneous anaphylaxis. 70% ethanol extract of strawberries doses 2.72 mg/20 g BW had similar antiallergic activity compare with cetirizine. The conclusion of this study showed that strawberries fruit extract could be developed as an alternative medicine to anti-anaphylaxis or anti-allergic.

scholarly journals Comparison of clinical, biomechanical, and histopathological effects of various suture techniques on repair of tendon rupture by using autograft

2017 ◽  
Vol 61 (2) ◽  
pp. 231-237
Author(s):  
Celal Şahin Ermutlu ◽  
Vedat Baran
Keyword(s):  
Good Alternative ◽  
Suture Technique ◽  
Suture Techniques ◽  
Tissue Loss ◽  
Group Iii ◽  
Interrupted Suture ◽  
Group I ◽  
Tendon Ruptures ◽  
The Right ◽  
And Control

AbstractIntroduction: The purpose of this study was to compare the effectiveness of four different suture techniques in the treatment of experimentally modelled tendon injuries with tissue loss with autograft and grafting applications in rabbits.Material and Methods: The study was performed on 30 male mature (2-year-old) New Zealand rabbits with mean body weight of 3.1 kg, divided into three equal groups. A graft measuring 1 cm in length was collected from the m. tibialis cranialis of each rabbit under general anaesthesia. The graft collected from the right tendon was transplanted into the left tendon, and the graft from the left tendon was transplanted into the right tendon. In all groups, a simple interrupted suture was placed on the left tendon as control, a Bunnell-Mayer suture was placed on the right tendon in group I, a Locking-Loop suture in group II, and a Horizontal U suture in group III. Both hindlimbs were bandaged for four weeks. The tendons were assessed biomechanically and histopathologically.Results: According to the results of the tensile testing, the maximum durability of the techniques ranked as follows: Bunnell-Mayer, Horizontal U, Locking-Loop, and control groups.Conclusion: The use of autografts was a good alternative for the treatment of tendon ruptures with tissue loss. Furthermore, even though there were no clinical or histopathological differences, the suture technique can be chosen based on the results of the tensile test.

Extrinsic Cell Infiltration and Revascularization Accelerate Mechanical Deterioration of the Patellar Tendon After Fibroblast Necrosis

2000 ◽  
Vol 122 (6) ◽  
pp. 594-599 ◽  
Author(s):  
Harukazu Tohyama ◽  
Kazunori Yasuda
Keyword(s):  
Mechanical Properties ◽  
Patellar Tendon ◽  
Cell Infiltration ◽  
Culture Study ◽  
Cell Culture Study ◽  
Group Iii ◽  
Freeze Thaw ◽  
Group I ◽  
Group Ii

This study was performed to determine the contribution of extrinsic cell infiltration and revascularization into the patellar tendon in alteration of the mechanical properties of the patellar tendon after intrinsic fibroblast necrosis using 77 rabbits. In Group I, after the patellar tendon underwent the in situ freeze-thaw treatment, a wrapping treatment was performed to inhibit any extrinsic cell infiltration into the tendon. In Group II, the patellar tendon underwent the freeze-thaw treatment without any of the wrapping treatment. In Group III, the patellar tendon underwent the same wrapping treatment but without any freeze-thaw treatment. The cell culture study demonstrated that the in situ freeze-thaw treatment killed from 97 to 100 percent of the cells in the patellar tendon. Histologically, no cells were found in the midsubstance of the patellar tendon in Group I at 1, 3, and 6 weeks. In Group II, a number of cells and some vessels were found scattered in the tendon at 3 and 6 weeks. Mechanically, the elastic modulus and the tensile strength of the patellar tendon of Group II were significantly lower than those of Groups I and III at 3 and 6 weeks. These facts suggest that extrinsic cell infiltration and revascularization from the surrounding tissues accelerate the deterioration of the mechanical properties of the patellar tendon matrix after intrinsic fibroblast necrosis. [S0148-0731(00)00506-9]

scholarly journals POTENSI ANTIBODI SPERMATOZOA TERHADAP SPERMATOGENESIS DAN FERTILISASI PADA TIKUS PUTIH

2007 ◽  
Vol 12 (2) ◽  
pp. 187-189
Author(s):  
Indah Norma Triana
Keyword(s):  
Group Iv ◽  
Male Rat ◽  
Group Iii ◽  
Rat Testis ◽  
Group I ◽  
Group Ii ◽  
And Control

The experiment was conducted the potential of antiibody sperm to spermatogenesis and fertility of the male rat. Ten of rat testis have been used in this research, then crushed and added with 10 cc saline and then were filtrated and centrifuged 1800 rpm for ten minutes. Twenty four of the male rat divided in to four groups. Group I injected with saline 0.1 cc/sc/day for 45 days, group II injected with testis suspension 0.1 cc/sc/day for 15 days, group III injected with testis suspension 0.1 cc/sc/days for 30 days, group IV injected with testis suspension 0.1 cc /sc/days for 45 days. Then each of the male rat were mated with female, then the number of the total of pups were counted. Male rat were killed and testis were collected for preparation of histology and count of the spermatocyte and spermatid. The results show that the number of the spermatocyte and spermatid has significant different in the group and control (p less than 0.05). The number of the pups significantly different in the group and control (p less than 0.05). Implication of this research was injected Antibodisperm on male rat can decrease of the number of spermatocyte, spermatid and the number of the pups.

scholarly journals 798 A Rare Complication and Unexpected Pathology Following an Anterior Exenteration – A Case Report

2021 ◽  
Vol 108 (Supplement_6) ◽  
Author(s):  
S Robertson ◽  
G Lazaraviciute ◽  
L Kerr ◽  
D Hendry
Keyword(s):  
Case Report ◽  
Small Bowel ◽  
Abdominal Wall ◽  
Abdominal Wall Defect ◽  
Rare Complication ◽  
Endometrial Adenocarcinoma ◽  
Abdominal Wall Reconstruction ◽  
Emergency Laparotomy ◽  
Proximal Thigh ◽  
Urachal Adenocarcinoma

Abstract We present a case report of an unexpected pathology in addition to a rare complication in a 62-year-old female following an anterior exenteration and an abdominal wall reconstruction. Initial biopsies characterised the malignancy as a potential urachal adenocarcinoma, but the final pathology revealed a solitary endometrial adenocarcinoma metastasis from endometrial cancer 6 years previously. Due to the nature of the tumour, an abdominal wall resection was required, and reconstruction involved a pedicled thigh flap. Post-operatively the small bowel herniated under the flap and this resulted in true mechanical small bowel obstruction. The loop of herniated bowel is visible on CT imaging in the area of the proximal thigh. The patient returned to theatre for an emergency laparotomy and the abdominal wall defect was closed instead with a surgical implant derived from animal tissue. The patient made an excellent recovery, was discharged home, and continues to do well. This patient is an example of excelling in adversity.

scholarly journals L-Carnitine reduces the negative effects of formalin on sperm parameters, chromatin condensation and apoptosis in mice: An experimental study

2020 ◽  
Author(s):  
Daniyal Ezati ◽  
Reyhane Vardiyan ◽  
Ali Reza Talebi ◽  
Morteza Anvari ◽  
Majid Pourentezari
Keyword(s):  
Experimental Study ◽  
Chromatin Condensation ◽  
Sperm Parameters ◽  
Sperm Chromatin ◽  
Protective Effects ◽  
Negative Effects ◽  
Group Iii ◽  
Group I ◽  
And Control

Background: Formalin is commonly applied as an antiseptic and tissue fixative. It has reactive molecules that lead to its cytotoxic effects. According to recent studies, formalin causes a change in the testicular and sperm structure and L-carnitine (LC) acts as an antioxidant to counteract its effects. Objectives: This study aimed to investigate the protective effects of LC on the parameters, chromatin condensation and apoptosis of mice sperm exposed to formalin. Materials and Methods: In this experimental study, 24 balb/c mice (25-40 gr ,10-12 wk) were divided into three groups (n = 8/each): group I without any injections or gavage; group II, received 10 mg/ kg formalin intraperitoneally (I.P); and group III was exposed to formalin and LC, where a dose of 10 mg/kg formalin was injected I.P daily and LC the dose of 100 mg/kg was kept in a solvent solution. After 31 days, the sperm examination was performed as follows: to evaluate chromatin and DNA quality of the sperm, we applied aniline blue (AB), toluidine blue (TB), chromomycin A3 (CMA3), and terminal transferase-mediated deoxy uridine triphosphate biotin end labeling (TUNEL) tests. Results: Sperm parameters such as count, motility, morphology, and viability displayed a significant decrease in the formalin group. While the data exhibited a considerable augment in sperm parameters in the formalin + LC than the formalin and control groups (p < 0.001), significant differences were detected between groups with respect to TB staining, TUNEL test, CMA3 test and AB staining in the formalin and formalin + LC groups. Conclusion: LC can reduce the negative effects of formalin on sperm parameters, chromatin stability, and percentage of apoptosis in an animal model. Key words: Formalin, L-carnitine, Mice, Sperm chromatin, Apoptosis.

scholarly journals Intra-Operative Total Bowel Washing: A New Method to Improve the Outcome of Gastroschisis Primary Repair

2020 ◽  
Vol 30 (3) ◽  
Author(s):  
Ahmad Mohammadipour ◽  
Mehran Hiradfar ◽  
Reza Shojaeian
Keyword(s):  
Gastrointestinal Tract ◽  
Abdominal Wall ◽  
Abdominal Wall Defect ◽  
Primary Repair ◽  
Abdominal Cavity ◽  
Abdominal Wall Reconstruction ◽  
Operation Time ◽  
Oral Feeding ◽  
Main Challenge ◽  
Significant Difference

Background: Gastroschisis is an abdominal wall defect that is managed by surgical reduction of herniated bowel into the abdominal cavity and abdominal wall reconstruction. Loss of abdominal domain is the main challenge that may complicate the process of gastroschisis management. Objectives: This article is about innovative manure called total bowel washing (TBW) that may improve the outcome of gastroschisis primary repair. Methods: All neonates with gastroschisis who met the study inclusion criteria between 2006 - 2019 were enrolled and divided into two groups of conventional and TBW method of gastroschisis management. In TBW group, bowls were washed with warm saline and after a gentle enterolysis, the whole gastrointestinal tract was irrigated via a gastric tube and evacuated completely from thick meconium until the watery stool started to come out of anus slightly. Primary abdominal wall closure was performed after loop by loop bowel reduction. Gastroschisis management outcome was compared between the two groups. Results: 15 neonates were allocated in each group. Demographic and anthropometric variables were compared and any significant difference wasn’t reported between the two groups. We observed a significantly better outcome in terms of faster GI rehabilitation, shorter time to oral feeding tolerance, less need to silo placement and shorter NICU and hospital stay in TBW method. Operation time was slightly longer in TBW group while the difference was not significant statistically. Conclusions: Total bowel washing and complete evacuation of gastrointestinal tract from thick meconium will increase the success rate of primary repair and improve the outcome of gastroschisis management.

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