scholarly journals The Lyophilization Process Maintains the Chemical and Biological Characteristics of Royal Jelly

2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Andresa Piacezzi Nascimento ◽  
Larissa Ariana Roveroni Moraes ◽  
Nathália Ursoli Ferreira ◽  
Gabriela de Padua Moreno ◽  
Fernanda Grassi Mangolini Uahib ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Fatty Acid ◽  
Royal Jelly ◽  
Antibacterial Properties ◽  
Chemical Characterization ◽  
Resistant Bacteria ◽  
Bioactive Substances ◽  
Antibiotic Resistant ◽  
Chromatographic Profile ◽  
Main Fatty Acid

The alternative use of natural products, like royal jelly (RJ), may be an important tool for the treatment of infections caused by antibiotic-resistant bacteria. RJ presents a large number of bioactive substances, including antimicrobial compounds. In this study, we carried out the chemical characterization of fresh and lyophilized RJ and investigated their antibacterial effects with the purpose of evaluating if the lyophilization process maintains the chemical and antibacterial properties of RJ. Furthermore, we evaluated the antibacterial efficacy of the main fatty acid found in RJ, the 10-hydroxy-2-decenoic acid (10H2DA). Chromatographic profile of the RJ samples showed similar fingerprints and the presence of 10H2DA in both samples. Furthermore, fresh and lyophilized RJ were effective against all bacteria evaluated; that is, the lyophilization process maintains the antibacterial activity of RJ and the chemical field of 10H2DA. The fatty acid 10H2DA exhibited a good antibacterial activity againstStreptococcus pneumoniae. Therefore, it may be used as an alternative and complementary treatment for infections caused by antibiotic-resistantS. pneumoniae.

scholarly journals Discovery of Antibacterial Dietary Spices That Target Antibiotic-Resistant Bacteria

2019 ◽  
Vol 7 (6) ◽  
pp. 157 ◽  
Author(s):  
Dan Zhang ◽  
Ren-You Gan ◽  
Arakkaveettil Kabeer Farha ◽  
Gowoon Kim ◽  
Qiong-Qiong Yang ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Antibacterial Effect ◽  
Antioxidant Activities ◽  
Antibacterial Properties ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Resistant Bacteria ◽  
Drug Resistant ◽  
Antibiotic Resistant Bacteria ◽  
Antibiotic Resistant ◽  
Dietary Spices

Although spice extracts are well known to exhibit antibacterial properties, there is lack of a comprehensive evaluation of the antibacterial effect of spices against antibiotic-resistant bacteria. In the present study, ethanolic extracts from a total of 67 spices were comprehensively investigated for their in vitro antibacterial activities by agar well diffusion against two common food-borne bacteria, Staphylococcus aureus and Salmonella enteritidis, with multi-drug resistance. Results showed that S. aureus was generally more sensitive to spice extracts than S. enteritidis. Of the 67 spice extracts, 38 exhibited antibacterial activity against drug-resistant S. aureus, while only four samples were effective on drug-resistant S. enteritidis. In addition, 11 spice extracts with inhibition zones greater than 15 mm were further verified for their broad-spectrum antibacterial properties using another 10 drug-resistant S. aureus strains. It was found that five spice extracts, including galangal, fructus galangae, cinnamon, yellow mustard seed, and rosemary, exhibited the highest antibacterial capacity. Further cytotoxicity of these 11 spices was determined and LC50 values were found to be more than 100 μg/mL except for galangal, rosemary, and sage, whose LC50 values were 9.32 ± 0.83, 19.77 ± 2.17, and 50.54 ± 2.57, respectively. Moreover, the antioxidant activities (ferric-reducing antioxidant power (FRAP) and trolox equivalent antioxidant capacity (TEAC) values) and total phenolic content (TPC) of spice extracts were determined to establish possible correlations with the antibacterial activity. Although the antibacterial effect was positively correlated with the antioxidant activities and TPC, the correlation was weak (r < 0.5), indicating that the antibacterial activity could also be attributed to other components besides antioxidant polyphenols in the tested spice extracts. In conclusion, dietary spices are good natural sources of antibacterial agents to fight against antibiotic-resistant bacteria, with potential applications as natural food preservatives and natural alternatives to antibiotics in animal feeding.

scholarly journals Naringenin as an antibacterial reagent controlling of biofilm formation and fatty acid metabolism in MRSA

2020 ◽  
Author(s):  
Hun-Suk Song ◽  
Shashi Kant Bhatia ◽  
Ranjit Gurav ◽  
Tae-Rim Choi ◽  
Hyun Joong Kim ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Fatty Acid ◽  
Biofilm Formation ◽  
Acid Metabolism ◽  
Resistant Bacteria ◽  
Beta Lactam ◽  
Biofilm Inhibition ◽  
Antibiotic Resistant ◽  
Beta Lactam Antibiotics

AbstractMRSA is Methicillin-resistant Staphylococcus aureus and they are widespread and making trouble in treatment in communities and surgical areas. MRSA have been adapted to antibiotics so that they can block the access of antibiotics physically or chemically deactivate it or modify the precursor of the target. Flavonoids are secondary metabolites which are naturally produced by plant or fungus and they are acting generally as pigment, quorum sensing molecules, antibiotics to other competitive microorganisms. Their natural origins and multiple activities have drawn much attention to be developed as the potential drugs since flavonoids could be a good candidate to overcome antibiotic resistant bacteria. Among various flavonoids, we found out naringenin has antibacterial activity on MRSA and Δagr mutants which are more resistance than MRSA to beta-lactam antibiotics by decreasing biofilm formation dramatically and decreasing the secretion of fatty acid. It also showed high synergetic activity with oxacillin to both antibacterial activity and biofilm inhibition. Considering the number of flavonoids, our experiments expand the possibility of the use of flavonoids to MRSA.

scholarly journals Chemical characterization and antibacterial activity of the essential oils of Tetraclinis articulata (Vahl) from Morocco

2019 ◽  
Vol 8 (5) ◽  
pp. 390-396
Author(s):  
Hannou Zerkani ◽  
Imane Tagnaout ◽  
Aziz Dirioiche ◽  
Imad Adadi ◽  
Jamila El Karkouri ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Antibacterial Properties ◽  
Chemical Characterization ◽  
Bornyl Acetate ◽  
Bioactive Substances ◽  
Phytochemical Study ◽  
Tetraclinis Articulata ◽  
Middle Atlas

The objective of this study was to identify new bioactive substances through the phytochemical study and evaluation of the antibacterial activity of the essential oils of Tetraclinis articulata (Vahl). Harvested in April 2016, in the region of Khenifra (Middle Atlas-Morocco). Separate of hydrodistillation extracts of the three organs (branches, leaves and fruits) of Tetraclinis articulate were performed. The yields of essential oils are 0.92% for fruits; 0.41% for branches and 0.61% for leaves. The GC/MS analysis allowed the identification of 33 compounds in essential oil leaves, 20 compounds in excess fruits and 58 compounds in branches. The main compounds are bornyl acetate (38.54%) and α-pinene (6.71%) for leaves. α-pinene (22.12%); 13-epi-mannol (3.58%) and retinol (3.44%) for branches. Bornyl acetate (19.96%); α-pinene (12.47%) and retinol (11.98%) for fruits. The antibacterial properties of the essential oil of leaves of Tetraclinis articulata (Vahl) were tested in clinical bacterial strain. The essential oils inhibit the growth of Escherichia coli, Staphylococcus aureus and Klebsiella pneumoniae at 1.2 µl/ml; 2.4 µl/ml and 9.7 µl/ml respectively. Indeed, the resistance of bacteria to antibiotics is known for its adverse effects on human health, to escape this problem, it is necessary to exploit the antibiotics from aromatic and medicinal plants.

scholarly journals New Hybrid Copper Nanoparticles/Conjugated Polyelectrolyte Composite with Antibacterial Activity

Polymers ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 401
Author(s):  
Ignacio A. Jessop ◽  
Yasmín P. Pérez ◽  
Andrea Jachura ◽  
Hipólito Nuñez ◽  
Cesar Saldías ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Copper Nanoparticles ◽  
Salmonella Enteritidis ◽  
Theoretical Calculations ◽  
Chemical Reduction ◽  
Metallic Copper ◽  
Resistant Bacteria ◽  
Small Contribution ◽  
Antibiotic Resistant ◽  
Conjugated Polyelectrolyte

In the search for new materials to fight against antibiotic-resistant bacteria, a hybrid composite from metallic copper nanoparticles (CuNPs) and a novel cationic π-conjugated polyelectrolyte (CPE) were designed, synthesized, and characterized. The CuNPs were prepared by chemical reduction in the presence of CPE, which acts as a stabilizing agent. Spectroscopic analysis and electron microscopy showed the distinctive band of the metallic CuNP surface plasmon and their random distribution on the CPE laminar surface, respectively. Theoretical calculations on CuNP/CPE deposits suggest that the interaction between both materials occurs through polyelectrolyte side chains, with a small contribution of its backbone electron density. The CuNP/CPE composite showed antibacterial activity against Gram-positive (Staphylococcus aureus and Enterococcus faecalis) and Gram-negative (Escherichia coli and Salmonella enteritidis) bacteria, mainly attributed to the CuNPs’ effect and, to a lesser extent, to the cationic CPE.

scholarly journals Antibacterial Activity of Moroccan Zantaz Honey and the Influence of Its Physicochemical Parameters Using Chemometric Tools

2021 ◽  
Vol 11 (10) ◽  
pp. 4675
Author(s):  
Youssef Elamine ◽  
Hamada Imtara ◽  
Maria Graça Miguel ◽  
Ofélia Anjos ◽  
Letícia M. Estevinho ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Antibacterial Properties ◽  
Principal Component ◽  
Physicochemical Characteristics ◽  
Multidrug Resistant ◽  
Resistant Bacteria ◽  
Coumaric Acid ◽  
Atlas Mountains ◽  
Methyl Syringate ◽  
Chemometric Tools

The emergence of multidrug-resistant bacteria has prompted the development of alternative therapies, including the use of natural products with antibacterial properties. The antibacterial properties of Zantaz honey produced in the Moroccan Atlas Mountains against Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus was evaluated and analyzed using chemometric tools. Minimum inhibitory concentration (MIC) and Minimum bactericidal concentration (MBC) against S. aureus were the lowest (112.5 ± 54.5 mg/mL), revealing that this species was most sensitive to Zantaz honey. P. aeruginosa showed an intermediate sensitivity (MIC= 118.75 ± 51.9 mg/mL), while E. coli was the most resistant to treatment (MIC = 175 ± 61.2 mg/mL). Content of monosaccharides, certain minerals, and phenolic compounds correlated with antibacterial activity (p < 0.05). Principal component analysis of physicochemical characteristics and antibacterial activity indicated that the parameters most associated with antibacterial activity were color, acidity, and content of melanoidins, fructose, epicatechin, methyl syringate, 4-coumaric acid, and 3-coumaric acid.

scholarly journals Antibacterial Activity of Prismatomeris tetrandra K. Schum Root Extract against Antibiotic Resistance Bacteria

2018 ◽  
Vol 16 (5) ◽  
pp. 341-348
Author(s):  
On-Anong SOMSAP
Keyword(s):  
Antibacterial Activity ◽  
Antibiotic Resistance ◽  
Culture Broth ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Root Extract ◽  
Gram Negative Bacteria ◽  
Antibiotic Resistant ◽  
Gram Positive Bacteria ◽  
Colony Forming Units

Antibiotic resistance bacteria has become an increasing problem now today due to many factors. This study investigates the efficacy of Prismatomeris tetrandra K. Schum root extract as a new source of antibacterial activity for antibiotic resistant bacteria using agar well diffusion method. The results showed that S. aureus TISTR517 exhibited more sensitivity to P. tetrandra K. Schum root extract than other Gram-positive bacteria indicator strains. On the other hand, Gram-negative bacteria exhibited resistance to P. tetrandra K. Schum root extract. The study further showed the activity between P. tetrandra K. Schum root extract and gentamycin (10 µg), it revealed that MRSA142 was resistant to gentamycin (10µg) but sensitive to P. tetrandra K. Schum root extract. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) was evaluated by using S. aureus TISTR517 and MRSA142 as indicator strains. The MIC value was 0.59 mg/mL and 1.17 mg/mL for S. aureus TISTR517 and MRSA142, respectively. MBC assay demonstrated that the MBC value was 9.75 mg/mL and 150 mg/mL for S. aureus TISTR517 and MRSA142 respectively. The mode of action was investigated with the presence of P. tetrandra K. Schum root extract in the culture broth. The action of P. tetrandra K. Schum root extract was revealed of bacteriostatic activity due to the Optical density (OD) at 600 nm and Colony-Forming Units (CFU) of indicator strains were continuously decreased.

scholarly journals A boronic acid-functionalized phthalocyanine with an aggregation-enhanced photodynamic effect for combating antibiotic-resistant bacteria

2020 ◽  
Vol 11 (22) ◽  
pp. 5735-5739 ◽  
Author(s):  
Eunhye Lee ◽  
Xingshu Li ◽  
Juwon Oh ◽  
Nahyun Kwon ◽  
Gyoungmi Kim ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Antibacterial Activity ◽  
Boronic Acid ◽  
Reactive Oxygen ◽  
Resistant Bacteria ◽  
Ros Generation ◽  
Oxygen Species ◽  
Photodynamic Effect ◽  
Antibiotic Resistant Bacteria ◽  
Antibiotic Resistant

A boronic acid functionalized phthalocyanine displays aggregation-enhanced reactive oxygen species (ROS) generation and excellent photodynamic antibacterial activity.

scholarly journals Antibacterial Activity of Volatile Organic Compounds Produced by the Octocoral-Associated Bacteria Bacillus sp. BO53 and Pseudoalteromonas sp. GA327

Antibiotics ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 923
Author(s):  
Anette Garrido ◽  
Librada A. Atencio ◽  
Rita Bethancourt ◽  
Ariadna Bethancourt ◽  
Héctor Guzmán ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Volatile Organic Compounds ◽  
Organic Compounds ◽  
Solid Phase ◽  
Resistant Bacteria ◽  
Bacillus Sp ◽  
Antibiotic Resistant Bacteria ◽  
Antibiotic Resistant ◽  
Associated Bacteria ◽  
Volatile Organic

The present research aimed to evaluate the antibacterial activity of volatile organic compounds (VOCs) produced by octocoral-associated bacteria Bacillus sp. BO53 and Pseudoalteromonas sp. GA327. The volatilome bioactivity of both bacteria species was evaluated against human pathogenic antibiotic-resistant bacteria, methicillin-resistant Staphylococcus aureus, Acinetobacter baumanni, and Pseudomonas aeruginosa. In this regard, the in vitro tests showed that Bacillus sp. BO53 VOCs inhibited the growth of P. aeruginosa and reduced the growth of S. aureus and A. baumanni. Furthermore, Pseudoalteromonas sp. GA327 strongly inhibited the growth of A. baumanni, and P. aeruginosa. VOCs were analyzed by headspace solid-phase microextraction (HS-SPME) joined to gas chromatography-mass spectrometry (GC-MS) methodology. Nineteen VOCs were identified, where 5-acetyl-2-methylpyridine, 2-butanone, and 2-nonanone were the major compounds identified on Bacillus sp. BO53 VOCs; while 1-pentanol, 2-butanone, and butyl formate were the primary volatile compounds detected in Pseudoalteromonas sp. GA327. We proposed that the observed bioactivity is mainly due to the efficient inhibitory biochemical mechanisms of alcohols and ketones upon antibiotic-resistant bacteria. This is the first report which describes the antibacterial activity of VOCs emitted by octocoral-associated bacteria.

Engineering highly effective antimicrobial selenium nanoparticles through control of particle size

Nanoscale ◽  
2019 ◽  
Vol 11 (31) ◽  
pp. 14937-14951 ◽  
Author(s):  
Tao Huang ◽  
James A. Holden ◽  
Daniel E. Heath ◽  
Neil M. O'Brien-Simpson ◽  
Andrea J. O'Connor
Keyword(s):  
Particle Size ◽  
Antibacterial Activity ◽  
Antibacterial Agent ◽  
Selenium Nanoparticles ◽  
Resistant Bacteria ◽  
Antibiotic Resistant Bacteria ◽  
Antibiotic Resistant ◽  
Size Dependent ◽  
Highly Effective

Selenium nanoparticles (Se NPs) are a promising antibacterial agent to address the challenge of antibiotic resistant bacteria. In this work, the antibacterial activity of the spherical Se NPs was shown to be strongly size dependent.

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