scholarly journals The Novel Functions of the PLC/PKC/PKD Signaling Axis in G Protein-Coupled Receptor-Mediated Chemotaxis of Neutrophils

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Xuehua Xu ◽  
Tian Jin
Keyword(s):  
Cell Migration ◽  
Signaling Pathway ◽  
G Protein ◽  
Essential Role ◽  
The Novel ◽  
G Protein Coupled Receptor ◽  
Signaling Axis ◽  
Extracellular Stimuli ◽  
Directional Cell Migration ◽  
G Protein Coupled

Chemotaxis, a directional cell migration guided by extracellular chemoattractant gradients, plays an essential role in the recruitment of neutrophils to sites of inflammation. Chemotaxis is mediated by the G protein-coupled receptor (GPCR) signaling pathway. Extracellular stimuli trigger activation of the PLC/PKC/PKD signaling axis, which controls several signaling pathways. Here, we concentrate on the novel functions of PLC/PKC/PKD signaling in GPCR-mediated chemotaxis of neutrophils.

scholarly journals Interactions of the G protein-coupled receptor-associated sorting proteins (GASP) 1 and 2 with the novel cannabinoid receptor GPR55

2008 ◽  
Vol 8 (Suppl 1) ◽  
pp. A16
Author(s):  
Julia Kargl ◽  
Nariman Balenga ◽  
Lene Martini ◽  
Jennifer Whistler ◽  
Maria Waldhoer
Keyword(s):  
G Protein ◽  
Cannabinoid Receptor ◽  
The Novel ◽  
G Protein Coupled Receptor ◽  
G Protein Coupled

scholarly journals Role of the G-Protein-Coupled Receptor Signaling Pathway in Insecticide Resistance

2019 ◽  
Vol 20 (17) ◽  
pp. 4300 ◽  
Author(s):  
Ting Li ◽  
Nannan Liu
Keyword(s):  
Insecticide Resistance ◽  
Cell Line ◽  
Signaling Pathway ◽  
G Protein ◽  
Cell Lines ◽  
Sf9 Cells ◽  
Camp Production ◽  
G Protein Coupled Receptor ◽  
G Protein Coupled

The G-protein-coupled receptor (GPCR) regulated intracellular signaling pathway is known to be involved in the development of insecticide resistance in the mosquito, Culex quinquefasciatus. To elucidate the specific role of each effector in the GPCR regulating pathway, we initially expressed a GPCR, G-protein alpha subunit (Gαs), adenylate cyclase (AC), and protein kinase A (PKA) in insect Spodoptera frugiperda (Sf9) cells and investigated their regulation function on cyclic AMP (cAMP) production and PKA activity. GPCR, Gαs, and AC individually expressed Sf9 cells showed higher cAMP production as the expression of each effector increased. All the effector-expressed cell lines showed increased PKA activity however. Moreover, Sf9 cytochrome P450 gene expression and cell tolerance to permethrin were examined. The relative expression of CYP9A32gene in Sf9 cells tested was significantly increased in all effector-expressed cell lines compared to a control cell line; these effector-expressed cell lines also showed significantly higher tolerance to permethrin. Inhibitor treatments on each effector-expressed cell line revealed that Bupivacaine HCl and H89 2HCl robustly inhibited cAMP production and PKA activity, respectively, resulting in decreased tolerance to permethrin in all cell lines. The synergistic functions of Bupivacaine HCl and H89 2HCl with permethrin were further examined in Culex mosquito larvae, providing a valuable new information for mosquito control strategies.

The novel G-protein coupled receptor SALPR shares sequence similarity with somatostatin and angiotensin receptors

Gene ◽  
2000 ◽  
Vol 248 (1-2) ◽  
pp. 183-189 ◽  
Author(s):  
Mitsuyuki Matsumoto ◽  
Masazumi Kamohara ◽  
Toru Sugimoto ◽  
Kazuyuki Hidaka ◽  
Jun Takasaki ◽  
...  
Keyword(s):  
G Protein ◽  
Sequence Similarity ◽  
Angiotensin Receptors ◽  
The Novel ◽  
G Protein Coupled Receptor ◽  
G Protein Coupled
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