scholarly journals The Antioxidant and Starch Hydrolase Inhibitory Activity of Ten Spices in anIn VitroModel of Digestion: Bioaccessibility of Anthocyanins and Carotenoids

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Nilakshi Jayawardena ◽  
Mindani I. Watawana ◽  
Ruchini T. Jayathilaka ◽  
Viduranga Y. Waisundara
Keyword(s):  
Total Phenolics ◽  
Seed Extract ◽  
Water Soluble ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
Star Anise ◽  
Curry Leaves ◽  
Inhibitory Activities ◽  
Mustard Seeds

The antioxidant and starch hydrolase inhibitory activities of cardamom, cloves, coriander, cumin seeds, curry leaves, fenugreek, mustard seeds, nutmeg, sweet cumin, and star anise extracts were investigated in anin vitromodel of digestion mimicking the gastric and duodenal conditions. The total phenolic contents in all spice extracts had statistically significantly (P<0.05) increased following both gastric and duodenal digestion. This was also in correlation with the antioxidant assays quantifying the water-soluble antioxidant capacity of the extracts. The lipophilic Oxygen Radical Absorbance Capacity assay did not indicate a statistically significant change in the values during any of the digestion phases. Statistically significant (P<0.05) reductions in the anthocyanin contents were observed during the digestion phases in contrast to the carotenoid contents. With the exception of the cumin seed extract, none of the spice extracts showed statistically significant changes in the initial starch hydrolase enzyme inhibitory values prior to gastric and duodenal digestion. In conclusion, this study was able to prove that the 10 spices were a significant source of total phenolics, antioxidant, and starch hydrolase inhibitory activities.

scholarly journals Pharmacognostic Standardization, Phenolic and in Vitro Antioxidant Activity of Silene villosa (Family: Caryophyllaceae)

2017 ◽  
Vol 9 (3) ◽  
pp. 106 ◽  
Author(s):  
Ahmed I. Foudah
Keyword(s):  
Antioxidant Activity ◽  
Methanol Extract ◽  
Antioxidant Property ◽  
Water Soluble ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Phenolic Contents ◽  
Total Phenolic Contents ◽  
Microscopic Features

The present study was designed to explore some of the quality principles and <em>in vitro</em> antioxidant exploration of <em>Silene villosa</em> “a non-intentional” herb. The pharmacognostical study and phytochemical evaluation of the herbs of <em>S. villosa</em> was carried out following the reported methods. The total phenolic contents and antioxidant potential of the methanol extract was also evaluated applying the standard methods. Microscopic features of the Transverse Section (T.S) of the root were observed as epidermis, medullary rays, xylem cells, phloem cells. The microscopic study of the powder showed the presence of spiral vessels, lignified fibre or tannin containing cells. The extractive value showed the water soluble materials were higher than the alcohol soluble materials. Primarily phytochemical analysis and total phenolic contents (11.78±0.169 %) in powder and extract respectively were conducted. The methanol extract of <em>S. villosa</em> showed dose dependent antioxidant activity. The present study helps in authentication of <em>S. villosa</em> and enhances further exploration of the health benefits due to the presence of phenolic contents and antioxidant property.

scholarly journals In Vitro Phytotherapeutic Properties of Aqueous Extracted Adenia viridiflora Craib. towards Civilization Diseases

Molecules ◽  
2021 ◽  
Vol 26 (4) ◽  
pp. 1082
Author(s):  
Werawat Wannasaksri ◽  
Nattira On-Nom ◽  
Chaowanee Chupeerach ◽  
Piya Temviriyanukul ◽  
Somsri Charoenkiatkul ◽  
...  
Keyword(s):  
Endangered Species ◽  
Local Population ◽  
Sinapic Acid ◽  
Growth Conditions ◽  
Total Phenolic ◽  
Edible Plant ◽  
Phenolic Contents ◽  
Plant Parts ◽  
Inhibitory Activities

Adenia viridiflora Craib. is an indigenous edible plant that became an endangered species due to limited consumption of the local population with unknown reproduction and growth conditions. The plant is used as a traditional herb; however, its health applications lack scientific-based evidence. A. viridiflora Craib. plant parts (old leaves and young shoots) from four areas as Kamphaeng Phet (KP), Muang Nakhon Ratchasima (MN), Pakchong Nakhon Ratchasima (PN), and Uthai Thani (UT) origins were investigated for phenolic compositions and in vitro health properties through the inhibition of key enzymes relevant to obesity (lipase), diabetes (α-glucosidase and dipeptidyl peptidase-IV), Alzheimer’s disease (cholinesterases and β-secretase), and hypertension (angiotensin-converting enzyme). Phenolics including p-coumaric acid, sinapic acid, naringenin, and apigenin were detected in old leaves and young shoots in all plant origins. Old leaves exhibited higher total phenolic contents (TPCs) and total flavonoid contents (TFCs), leading to higher enzyme inhibitory activities than young shoots. Besides, PN and MN with higher TPCs and TFCs tended to exhibit greater enzyme inhibitory activities than others. These results will be useful to promote this plant as a healthy food with valuable medicinal capacities to support its consumption and agricultural stimulation, leading to sustainable conservation of this endangered species.

scholarly journals Evaluation of phenolic compounds and antioxidant activity in some edible flowers

2020 ◽  
Vol 5 (1) ◽  
pp. 857-870
Author(s):  
Fernando Gonçalves ◽  
João Carlos Gonçalves ◽  
Ana Cristina Ferrão ◽  
Paula Correia ◽  
Raquel P. F. Guiné
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolics ◽  
Total Phenolic ◽  
Potential Health ◽  
Phenolic Contents ◽  
Spectrophotometric Methods ◽  
Potential Source ◽  
Successive Extraction ◽  
Dpph Method ◽  
Total Anthocyanins

AbstractRecently, edible flowers (EF) have aroused increased interest because of their aesthetic properties as well as potential health benefits related to the occurrence of some bioactive compounds. Therefore, the aim of this work was to evaluate the total phenolics, anthocyanins, flavonoids, and antioxidant activity (AOA) (following DPPH and ABTS methods) in eleven EF. The samples were subjected to three successive extraction steps using methanol, and these extracts were then analysed for the aforementioned properties using spectrophotometric methods. The obtained extracts were used for the quantification of phenolic composition and AOA. The results indicated that, among the flowers analysed in this study, red rose, pink rose, and red carnation possessed the highest total phenolic contents (27.53, 23.30, and 18.17 mg g−1 gallic acid equivalents, respectively), total anthocyanins (3.07, 1.97, and 4.47 mg g−1 catechin equivalents [CE], respectively), and AOA (12.07, 15.77, and 12.93 mg g−1 TE, respectively, as given by the DPPH method or 8.23, 9.27 and 8.00 mg g−1 TE, respectively, as given by the ABTS method). The flowers with highest flavonoids contents were red carnation, Mexican marigold, and pink rose (17.50, 16.90, and 16.57 mg g−1 CE, respectively). Cluster analysis grouped the analysed flowers into two groups, those richest in phenolics with AOA and those not so rich. Finally, some important correlations were observed between the total phenolics and the AOA. In conclusion, these flowers could represent a potential source of natural compounds with antioxidant capacity.

scholarly journals In Vitro α-Glucosidase and α-Amylase Inhibitory Activities of Free and Bound Phenolic Extracts from the Bran and Kernel Fractions of Five Sorghum Grain Genotypes

Foods ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 1301
Author(s):  
Yun Xiong ◽  
Ken Ng ◽  
Pangzhen Zhang ◽  
Robyn Dorothy Warner ◽  
Shuibao Shen ◽  
...  
Keyword(s):  
Digestive System ◽  
Inhibitory Concentration ◽  
Inhibitory Effect ◽  
Inhibitory Effects ◽  
Phenolic Contents ◽  
Inhibitory Activities ◽  
Sorghum Grain ◽  
Global Health Challenge ◽  
Phenolic Extracts

Diabetes is a global health challenge. Currently, an effective treatment for diabetes is to reduce the postprandial hyperglycaemia by inhibiting the carbohydrate hydrolysing enzymes in the digestive system. In this study, we investigated the in vitro α-glucosidase and α-amylase inhibitory effects of free and bound phenolic extracts, from the bran and kernel fractions of five sorghum grain genotypes. The results showed that the inhibitory effect of sorghum phenolic extracts depended on the phenolic concentration and composition. Sorghum with higher phenolic contents generally had higher inhibitory activity. Among the tested extracts, the brown sorghum (IS131C)-bran-free extract (BR-bran-free, half-maximal inhibitory concentration (IC50) = 18 ± 11 mg sorghum/mL) showed the strongest inhibition against α-glucosidase which was comparable to that of acarbose (IC50 = 1.39 ± 0.23 mg acarbose/mL). The red sorghum (Mr-Buster)-kernel-bound extract (RM-kernel-bound, IC50 = 160 ± 12 mg sorghum/mL) was the most potent in inhibiting α-amylase but was much weaker compared to acarbose (IC50 = 0.50 ± 0.03 mg acarbose/mL).

scholarly journals IN VITRO TOTAL PHENOLICS, FLAVONOIDS CONTENTS, ANTIOXIDANT AND ANTIMICROBIAL ACTIVITES OF VARIOUS SOLVENT EXTRACTS FROM THE MEDICINAL PLANT PHYSALIS MINIMA LINN

2017 ◽  
Vol 9 (3) ◽  
pp. 192 ◽  
Author(s):  
Venkanna Banothu ◽  
Uma Adepally ◽  
Jayalakshmi Lingam
Keyword(s):  
Medicinal Plant ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Total Phenolics ◽  
Antioxidant Properties ◽  
Dry Weight ◽  
Aluminium Chloride ◽  
Total Phenolic ◽  
Antioxidant Power

Objective: To estimate the in vitro total phenolics, flavonoids contents, antioxidant and antimicrobial activities of various solvent extracts from the medicinal plant Physalis minima Linn.Methods: The crude bioactive were extracted from the dried powder of Physalis minima using methanol, ethyl acetate, chloroform and hexane solvents. Total phenolic content (TPC) and total flavonoid content (TFC) were estimated using Folin-Ciocalteu and aluminium chloride colorimetric methods respectively. 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2’-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and ferric reducing antioxidant power (FRAP) assays were used to determine the in vitro antioxidant capacity. The antimicrobial assay was done through agar well diffusion; minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined using broth microdilution methods against the Gram-negative bacteria (Klebsiella pneumoniae, Escherichia coli, Pseudomonas aeruginosa, Proteus vulgaris) and Gram-positive bacteria (Staphylococcus aureus).Results: TPC expressed as gallic acid equivalents (GAE) ranged from 60.27±1.73-151.25±2.50 mg GAE/g dry weight, and TFC expressed as quercetin equivalents (QE) ranged from 56.66±0.80-158.84±2.30 mg QE/g dry weight. Methanol extract showed the highest antioxidant activity followed by ethyl acetate, chloroform, hexane extract and the IC50 values of methanol extract for scavenging DPPH and ABTS free radicals were 280.23±5.75-173.40±0.38µg/ml, respectively. All the extracts have shown potent antimicrobial activity for the zone of inhibition ranged from 9-35 mm; MICs and MBCs values ranged from 0.125-4.0 and 0.25-8.0 mg/ml, respectively towards tested pathogenic species.Conclusion: The comprehensive analysis of the present results demonstrated that Physalis minima possess high potential antioxidant properties which could be used as a viable source of natural antioxidants in treating infections caused by above-mentioned pathogens.

scholarly journals RP-HPLC based evidences of rich sources of Phenolics and water-soluble vitamins in an annual sedge Cyperus compressus

2018 ◽  
Vol 7 (3) ◽  
pp. 305-311
Author(s):  
Sudeshna Datta ◽  
◽  
Tapan Seal ◽  
BK Sinha ◽  
Soumen Bhattacharjee ◽  
...  
Keyword(s):  
Pantothenic Acid ◽  
Ethanol Extract ◽  
Solvent System ◽  
Scientific Basis ◽  
Water Soluble ◽  
Total Phenolic ◽  
Antioxidant Compounds ◽  
Vitamin Supplement ◽  
Excellent Source

The present study makes an effort to evaluate the content of water-soluble vitamins like ascorbic acid (C), thiamine (B1), riboflavin (B2), niacin (B3), pantothenic acid (B5), pyridoxine (B6) and folic acid (B9) by HPLC of Cyperus compressus. The in vitro antioxidant activity was carried out in different solvent system of varying polarity. The 70% ethanol appeared to be the most potent solvent for extraction of antioxidant compounds. The total phenolic and flavonoid content were found 72.544 ± 0.144 mg/g dm and 12.930 ± 0.144 mg/g dm respectively in the plant. Simultaneous HPLC fingerprinting of some standard phenolic acids and flavonoids in the 70 % aqueous ethanol extract was also carried out. B vitamins ranged between 0.028 to 0.431 mg/100g dm. The results confirm that this annual sedge is an excellent source of antioxidant- based phytonutrients, which could be used for the good of human being at the same time it can be used as a vitamin supplement to our diet. The study also provides the scientific basis of traditional significance of Cyperus compressus

Total phenolic contents of 33 fruits and their antioxidant capacities before and after in vitro digestion

2014 ◽  
Vol 57 ◽  
pp. 150-157 ◽  
Author(s):  
Guan-Lin Chen ◽  
Song-Gen Chen ◽  
Ying-Ying Zhao ◽  
Chun-Xia Luo ◽  
Juan Li ◽  
...  
Keyword(s):  
In Vitro Digestion ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
Total Phenolic Contents ◽  
Antioxidant Capacities ◽  
Before And After

scholarly journals Phenolic Contents and Antioxidant Potential ofCrataegusFruits Grown in Tunisia as Determined by DPPH, FRAP, andβ-Carotene/Linoleic Acid Assay

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Farouk Mraihi ◽  
Mohamed Journi ◽  
Jamila Kalthoum Chérif ◽  
Munevver Sokmen ◽  
Atalay Sokmen ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Linoleic Acid ◽  
Natural Antioxidants ◽  
Total Phenolic ◽  
Frap Assay ◽  
Phenolic Contents ◽  
Dpph Method ◽  
Total Anthocyanins ◽  
Linoleic Acid Assay

Crataegusfruit is one of most important fruits in Tunisian flora. Some fruits of this genus are edible. This study was undertaken in order to examine the benefits of these fruits in human health and their composition of antioxidants including total polyphenol, flavonoids, proanthocyanidins content, and total anthocyanins. The antioxidative properties of the ultrasonic methanolic extract were assessed by different in vitro methods such as the FRAP, DPPH, andβ-carotene/linoleic acid assay. We concluded that peel fraction of red fruits possessed relatively high antioxidant activity and might be a rich source of natural antioxidants in comparison with the pulp and seed fruit extract. The results also showed that hawthorn yellow fruit presents lower amounts of phenolic content, absence of anthocyanins, and less antioxidant capacity. Most of peel and seed fractions were stronger than the pulp fractions in antioxidant activity based on their DPPH IC50, FRAP values, and results ofβ-carotene/linoleic acid. The total phenolic compounds contents were also highly correlated with the DPPH method and the FRAP assay.

Antioxidant and acetylcholinesterase inhibitory activities of ginger root (Zingiber officinale Roscoe) extract

2017 ◽  
Vol 14 (4) ◽  
Author(s):  
Bui Thanh Tung ◽  
Dang Kim Thu ◽  
Nguyen Thi Kim Thu ◽  
Nguyen Thanh Hai
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Zingiber Officinale ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
Zingiber Officinale Roscoe ◽  
Etoac Fraction ◽  
Inhibitory Activities ◽  
Acetylcholinesterase Enzyme

AbstractBackgroundRoscoe has been used in traditional medicine for the treatment of neurological disorder. This study aimed to investigate the phenolic contents, antioxidant, acetylcholinesterase enzyme (AChE) inhibitory activities of different fraction ofMethodsThe roots ofResultsOur data showed that the total phenolic content of EtOAc fraction was highest equivalents to 35.2±1.4 mg quercetin/g of fraction. Our data also demonstrated that EtOAc fraction had the strongest antioxidant activity with ICConclusionsOur results suggest that the EtOAc fraction of

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