scholarly journals Characterization of Bone Marrow Mononuclear Cells on Biomaterials for Bone Tissue EngineeringIn Vitro

2015 ◽  
Vol 2015 ◽  
pp. 1-12 ◽  
Author(s):  
Dirk Henrich ◽  
René Verboket ◽  
Alexander Schaible ◽  
Kerstin Kontradowitz ◽  
Elsie Oppermann ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Tissue ◽  
Surface Coating ◽  
Mononuclear Cells ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Bone Marrow Mononuclear Cells ◽  
Beta Tricalcium Phosphate ◽  
Initial Adhesion

Bone marrow mononuclear cells (BMCs) are suitable for bone tissue engineering. Comparative data regarding the needs of BMC for the adhesion on biomaterials and biocompatibility to various biomaterials are lacking to a large extent. Therefore, we evaluated whether a surface coating would enhance BMC adhesion and analyze the biocompatibility of three different kinds of biomaterials. BMCs were purified from human bone marrow aspirate samples. Beta tricalcium phosphate (β-TCP, without coating or coated with fibronectin or human plasma), demineralized bone matrix (DBM), and bovine cancellous bone (BS) were assessed. Seeding efficacy onβ-TCP was 95% regardless of the surface coating. BMC demonstrated a significantly increased initial adhesion on DBM andβ-TCP compared to BS. On day 14, metabolic activity was significantly increased in BMC seeded on DBM in comparison to BMC seeded on BS. Likewise increased VEGF-synthesis was observed on day 2 in BMC seeded on DBM when compared to BMC seeded on BS. The seeding efficacy of BMC on uncoated biomaterials is generally high although there are differences between these biomaterials. Beta-TCP and DBM were similar and both superior to BS, suggesting either as suitable materials for spatial restriction of BMC used for regenerative medicine purposesin vivo.

scholarly journals A novel gelatin/chitooligosaccharide/demineralized bone matrix composite scaffold and periosteum-derived mesenchymal stem cells for bone tissue engineering

2021 ◽  
Vol 25 (1) ◽  
Author(s):  
Thakoon Thitiset ◽  
Siriporn Damrongsakkul ◽  
Supansa Yodmuang ◽  
Wilairat Leeanansaksiri ◽  
Jirun Apinun ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Formation ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Alp Activity

Abstract Background A novel biodegradable scaffold including gelatin (G), chitooligosaccharide (COS), and demineralized bone matrix (DBM) could play a significant part in bone tissue engineering. The present study aimed to investigate the biological characteristics of composite scaffolds in combination of G, COS, and DBM for in vitro cell culture and in vivo animal bioassays. Methods Three-dimensional scaffolds from the mixture of G, COS, and DBM were fabricated into 3 groups, namely, G, GC, and GCD using a lyophilization technique. The scaffolds were cultured with mesenchymal stem cells (MSCs) for 4 weeks to determine biological responses such as cell attachment and cell proliferation, alkaline phosphatase (ALP) activity, calcium deposition, cell morphology, and cell surface elemental composition. For the in vivo bioassay, G, GC, and GCD, acellular scaffolds were implanted subcutaneously in 8-week-old male Wistar rats for 4 weeks and 8 weeks. The explants were assessed for new bone formation using hematoxylin and eosin (H&E) staining and von Kossa staining. Results The MSCs could attach and proliferate on all three groups of scaffolds. Interestingly, the ALP activity of MSCs reached the greatest value on day 7 after cultured on the scaffolds, whereas the calcium assay displayed the highest level of calcium in MSCs on day 28. Furthermore, weight percentages of calcium and phosphorus on the surface of MSCs after cultivation on the GCD scaffolds increased when compared to those on other scaffolds. The scanning electron microscopy images showed that MSCs attached and proliferated on the scaffold surface thoroughly over the cultivation time. Mineral crystal aggregation was evident in GC and greatly in GCD scaffolds. H&E staining illustrated that G, GC, and GCD scaffolds displayed osteoid after 4 weeks of implantation and von Kossa staining confirmed the mineralization at 8 weeks in G, GC, and GCD scaffolds. Conclusion The MSCs cultured in GCD scaffolds revealed greater osteogenic differentiation than those cultured in G and GC scaffolds. Additionally, the G, GC, and GCD scaffolds could promote in vivo ectopic bone formation in rat model. The GCD scaffolds exhibited maximum osteoinductive capability compared with others and may be potentially used for bone regeneration.

scholarly journals Corrigendum to “Characterization of Bone Marrow Mononuclear Cells on Biomaterials for Bone Tissue EngineeringIn Vitro”

2015 ◽  
Vol 2015 ◽  
pp. 1-1
Author(s):  
Dirk Henrich ◽  
René Verboket ◽  
Alexander Schaible ◽  
Kerstin Kontradowitz ◽  
Elsie Oppermann ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Tissue ◽  
Mononuclear Cells ◽  
Bone Marrow Mononuclear Cells

Demineralized bone matrix-based microcarrier scaffold favors vascularized large bone regeneration in vivo in a rat model

2018 ◽  
Vol 33 (2) ◽  
pp. 182-195 ◽  
Author(s):  
Qiannan Li ◽  
Wenjie Zhang ◽  
Guangdong Zhou ◽  
Yilin Cao ◽  
Wei Liu ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Stem Cells ◽  
Bone Marrow ◽  
Bone Regeneration ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Matrix Group ◽  
Micro Computed Tomography ◽  
Demineralized Bone

Insufficient neo-vascularization of in vivo implanted cell-seeded scaffold remains a major bottleneck for clinical translation of engineered bone formation. Demineralized bone matrix is an ideal bone scaffold for bone engineering due to its structural and biochemical components similar to those of native bone. We hypothesized that the microcarrier form of demineralized bone matrix favors ingrowth of vessels and bone regeneration upon in vivo implantation. In this study, a rat model of femoral vessel pedicle-based bone engineering was employed by filling the demineralized bone matrix scaffolds inside a silicone chamber that surrounded the vessel pedicles, and to compare the efficiency of vascularized bone regeneration between microcarrier demineralized bone matrix and block demineralized bone matrix. The results showed that bone marrow stem cells better adhered to microcarrier demineralized bone matrix and produced more extracellular matrices during in vitro culture. After in vivo implantation, microcarrier demineralized bone matrix seeded with bone marrow stem cells formed relatively more bone tissue than block demineralized bone matrix counterpart at three months upon histological examination. Furthermore, micro-computed tomography three-dimensional reconstruction showed that microcarrier demineralized bone matrix group regenerate significantly better and more bone tissues than block demineralized bone matrix both qualitatively and quantitatively (p < 0.05). Moreover, micro-computed tomography reconstructed angiographic images also demonstrated significantly enhanced tissue vascularization in microcarrier demineralized bone matrix group than in block demineralized bone matrix group both qualitatively and quantitatively (p < 0.05). Anti-CD31 immunohistochemical staining of (micro-) vessels and semi-quantitative analysis also evidenced enhanced vascularization of regenerated bone in microcarrier demineralized bone matrix group than in block demineralized bone matrix group (p < 0.05). In conclusion, the microcarrier form of demineralized bone matrix is an ideal bone regenerative scaffold due to its advantages of osteoinductivity and vascular induction, two essentials for in vivo bone regeneration.

The effects of demineralized bone matrix and direct current on an ?in vivo? culture of bone marrow cells

1989 ◽  
Vol 7 (1) ◽  
pp. 22-27 ◽  
Author(s):  
Zachary B. Friedenberg ◽  
Carl T. Brighton ◽  
James D. Michelson ◽  
John Bednar ◽  
Richard Schmidt ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Direct Current ◽  
Bone Marrow Cells ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
In Vivo Culture ◽  
Marrow Cells ◽  
Demineralized Bone

PM373 In Vivo Assessment of Biodistribution and Efficacy of Bone Marrow Mononuclear Cells for Ischaemia-mediated Neovascularisation

Global Heart ◽  
2014 ◽  
Vol 9 (1) ◽  
pp. e137
Author(s):  
Sui Ching G. Yuen ◽  
Zoe Clayton ◽  
Ashanti Dantanarayana ◽  
Laura Lecce ◽  
Louise Dunn ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Mononuclear Cells ◽  
Bone Marrow Mononuclear Cells ◽  
In Vivo Assessment

scholarly journals Comparing Three Different Three-dimensional Scaffolds for Bone Tissue Engineering: An in vivo Study

2015 ◽  
Vol 16 (1) ◽  
pp. 25-30 ◽  
Author(s):  
Saeid Nosouhian ◽  
Amin Davoudi ◽  
Mansour Rismanchian ◽  
Sayed Mohammad Razavi ◽  
Hamidreza Sadeghiyan
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Three Dimensional ◽  
Control Group ◽  
Lamellar Bone ◽  
Significant Difference

ABSTRACT Introduction Three-dimensional Scaffold structure of synthetic biomaterials with their interconnected spaces seem to be a safe and effective option in supporting bone regeneration. The aim of this animal study was to compare the effectiveness of three different biocompatible scaffolds: bioglass (BG), demineralized bone matrix (DBM) and forstrite (FR). Materials and methods Four healthy dogs were anesthetized and the first to fourth premolars were extracted atraumatically in each quadrant. After healing, linear incision was prepared from molar to anterior segment and 4 defects in each quadrant (16 defects in each dog) were prepared. Scaffold blocks of BG, DBM and FR were resized according to size of defects and placed in the 12 defects randomly, 4 defects remained as control group. The dogs were sacrificed in 4 time intervals (15, 30, 45 and 60 days after) and the percentage of different types of regenerated bones (lamellar and woven) and connective tissue were recorded in histological process. The data were analyzed by one-way ANOVA and post hoc using SPSS software Ver. 15 at significant level of 0.05. Results In day 30th, although the amount of regenerated lamellar bone in control, DBM and BG Scaffold (22.37 ± 3.44; 21.46 ± 1.96; 21.21 ± 0.96) were near to each, the FR Scaffold provided the highest amount of lamellar (29.71 ± 7.94) and woven bone (18.28 ± 2.35). Also, FR Scaffold showed significant difference with BG (p = 0.026) and DBM Scaffolds (p = 0.032) in regenerated lamellar bone. Conclusion We recommend paying more attention to FR Scaffold as a biomaterial, but it is better to be compared with other nano biomaterials in future studies. How to cite this article Rismanchian M, Nosouhian S, Razavi SM, Davoudi A, Sadeghiyan H. Comparing Three Different Threedimensional Scaffolds for Bone Tissue Engineering: An in vivo Study. J Contemp Dent Pract 2015;16(1):25-30.

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