scholarly journals A Pyrosequencing Assay for the Quantitative Methylation Analysis ofGALR1in Endometrial Samples: Preliminary Results

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Christine Kottaridi ◽  
Nikolaos Koureas ◽  
Niki Margari ◽  
Emmanouil Terzakis ◽  
Evripidis Bilirakis ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Genital Tract ◽  
Critical Role ◽  
Female Genital Tract ◽  
Methylation Analysis ◽  
Preliminary Results ◽  
Pyrosequencing Assay ◽  
Aberrant Dna Methylation ◽  
Common Malignancy ◽  
Female Genital

Endometrial cancer is the most common malignancy of the female genital tract while aberrant DNA methylation seems to play a critical role in endometrial carcinogenesis. Galanin’s expression has been involved in many cancers. We developed a new pyrosequencing assay that quantifies DNA methylation of galanin’s receptor-1 (GALR1). In this study, the preliminary results indicate that pyrosequencing methylation analysis ofGALR1promoter can be a useful ancillary marker to cytology as the histological status can successfully predict. This marker has the potential to lead towards better management of women with endometrial lesions and eventually reduce unnecessary interventions. In addition it can provide early warning for women with negative cytological result.

scholarly journals The Microbiome as a Key Regulator of Female Genital Tract Barrier Function

2021 ◽  
Vol 11 ◽  
Author(s):  
Andrew Plesniarski ◽  
Abu Bakar Siddik ◽  
Ruey-Chyi Su
Keyword(s):  
Barrier Function ◽  
Genital Tract ◽  
Critical Role ◽  
Female Genital Tract ◽  
Host Cells ◽  
Mucosal Barrier ◽  
Host Immune System ◽  
Soluble Factors ◽  
Mucosal Barrier Function ◽  
Female Genital

The microbiome, the collection of microbial species at a site or compartment, has been an underappreciated realm of human health up until the last decade. Mounting evidence suggests the microbiome has a critical role in regulating the female genital tract (FGT) mucosa’s function as a barrier against sexually transmitted infections (STIs) and pathogens. In this review, we provide the most recent experimental systems and studies for analyzing the interplay between the microbiome and host cells and soluble factors with an influence on barrier function. Key components, such as microbial diversity, soluble factors secreted by host and microbe, as well as host immune system, all contribute to both the physical and immunologic aspects of the FGT mucosal barrier. Current gaps in what is known about the effects of the microbiome on FGT mucosal barrier function are compared and contrasted with the literature of the gut and respiratory mucosa. This review article presents evidence supporting that the vaginal microbiome, directly and indirectly, contributes to how well the FGT protects against infection.

scholarly journals Controversies in the Management of Endometrial Cancer

2010 ◽  
Vol 2010 ◽  
pp. 1-7 ◽  
Author(s):  
V. Masciullo ◽  
G. Amadio ◽  
D. Lo Russo ◽  
I. Raimondo ◽  
A. Giordano ◽  
...  
Keyword(s):  
Endometrial Cancer ◽  
Genital Tract ◽  
Female Genital Tract ◽  
Abnormal Uterine Bleeding ◽  
Uterine Bleeding ◽  
Curative Therapy ◽  
Sixth Decade ◽  
Common Malignancy ◽  
Female Genital

Endometrial cancer (EC) remains the most common malignancy of the female genital tract. The median age at diagnosis is the sixth decade, with abnormal uterine bleeding at the presentation in 90% of the patients. Surgical treatment, including complete hysterectomy, removal of remaining adnexal structures, and an appropriate surgical staging, represents the milestone of curative therapy for patients with EC. Adjuvant therapy is necessary in patients at high risk of recurrence. Conservative treatment approaches should be used in selected cases for women with a desire of fertility preservation. This review summarizes the management of EC and discusses current controversies regarding the role of lymphadenectomy and radiotherapy in patients with intermediate-risk tumors confined to the uterus.

Sign in / Sign up

Export Citation Format

Share Document