scholarly journals Effects of Chitosan Concentration on the Protein Release Behaviour of Electrospun Poly(ε-caprolactone)/Chitosan Nanofibers

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
Fatemeh Roozbahani ◽  
Naznin Sultana ◽  
Davood Almasi ◽  
Farnaz Naghizadeh
Keyword(s):  
Fiber Diameter ◽  
Morphological Characteristics ◽  
Solvent System ◽  
Release Profile ◽  
Average Fiber Diameter ◽  
Blend Ratio ◽  
Chitosan Concentration ◽  
Nanofibrous Scaffolds ◽  
Model Protein ◽  
Scanning Electron

Poly(ε-caprolactone)/chitosan (PCL/chitosan) blend nanofibers with different ratios of chitosan were electrospun from a formic acid/acetic acid (FA/AA) solvent system. Bovine serum albumin (BSA) was used as a model protein to incorporate biochemical cues into the nanofibrous scaffolds. The morphological characteristics of PCL/chitosan and PCL/chitosan/BSA Nanofibers were investigated by scanning electron microscopy (SEM). Fourier transform infrared spectroscopy (FTIR) was used to detect the presence of polymeric ingredients and BSA in the Nanofibers. The effects of the polymer blend ratio and BSA concentration on the morphological characteristics and consequently on the BSA release pattern were evaluated. The average fiber diameter and pore size were greater in Nanofibers containing BSA. The chitosan ratio played a significant role in the BSA release profile from the PCL/chitosan/BSA blend. Nanofibrous scaffolds with higher chitosan ratios exhibited less intense bursts in the BSA release profile.

Effects of Solution Parameters on Morphology and Diameter of Electrospun Polystyrene Nanofibers

2011 ◽  
Vol 194-196 ◽  
pp. 629-632 ◽  
Author(s):  
Todsapon Nitanan ◽  
Praneet Opanasopit ◽  
Prasert Akkaramongkolporn ◽  
Theerasak Rojanarata ◽  
Tanasait Ngawhirunpat
Keyword(s):  
Mixed Solvent ◽  
Fiber Diameter ◽  
Average Diameter ◽  
Solvent System ◽  
Diameter Distribution ◽  
Solution Properties ◽  
Fiber Diameter Distribution ◽  
Solution Parameters ◽  
Scanning Electron ◽  
Spun Fibers

This study focused on the preparation of electrospun polystyrene (PS) nanofibers. Polystyrene solutions were prepared in single (dimethylformamide; DMF, dimethylacetamide; DMAc or tetrahydrofuran; THF) and mixed solvent (DMF/THF and DMAc/THF) systems prior to electrospinning. The effects of solution parameters, including PS concentration and solvent system on solution properties (e.g. conductivity and viscosity), appearance and diameter of polystyrene fibers were examined. The morphology of the as-spun fibers were carefully investigated using scanning electron microscopy (SEM). It was found that the average diameter of the as-spun fibers increased upon increasing PS concentration. Moreover, the morphology of the fibers significantly depended on the properties of the solvents. The obtained fibers were smooth without any beads and their diameters depended on the amount of THF in the mixed solvent and PS concentration. In summary, the smallest diameter (927±81 nm) and the narrowest fiber diameter distribution of PS nanofibers were obtained from 15% PS solution in DMF/THF (75/25).

scholarly journals Engineering Biomimetic Gelatin Based Nanostructures as Synthetic Substrates for Cell Culture

2019 ◽  
Vol 9 (8) ◽  
pp. 1583
Author(s):  
Shaleena K. Pazhanimala ◽  
Driton Vllasaliu ◽  
Bahijja T. Raimi-Abraham
Keyword(s):  
Cell Proliferation ◽  
Fiber Diameter ◽  
Cost Effective ◽  
Solvent System ◽  
Attenuated Total Reflection ◽  
Force Microscopy ◽  
Membrane Topography ◽  
Nanofibrous Scaffolds ◽  
A Cell

There is a need for synthetic substrates that replicate the natural environment for in vitro intestinal models. Electrospinning is one of the most versatile and cost-effective techniques to produce nanofibrous scaffolds mimicking the basement membrane topography. In this study, three different novel electrospun nanofibrous scaffolds made of a polycaprolactone (PCL), gelatin, and poloxamer 188 (P188) blend were produced and compared with PCL and PCL/gelatin fibers produced using the same solvent system and electrospinning parameters. Each polymer solution used in this experiment was electrospun at four different voltages to study its influence on fiber diameter. The morphology and physical characteristics of the fibers were studied using scanning electron microscopy and atomic force microscopy. The average fiber diameter of all scaffolds was within 200–600 nm and no significant decrease in diameter with an increase in voltage was observed. Attenuated total reflection Fourier transform infrared spectroscopy was used to determine the chemical characteristics of the nanofibrous scaffold. The conductivity of the polymer solutions was also analyzed. Biocompatibility of the scaffolds was determined by a cell proliferation study performed using colorectal carcinoma (Caco-2) cells. PCL/gelatin/P188 scaffolds exhibited higher cell proliferation compared to PCL, PCL/gelatin scaffolds, and the control (tissue culture multi-well plate) with PCL/gelatin/P188 80:10:10 sample showing the highest cell proliferation.

Characterization and Multi-Response Morphological Optimization for Preparation of Defect-Free Electrospun Nanofibers Using the Taguchi Method

2017 ◽  
Vol 30 ◽  
pp. 61-75 ◽  
Author(s):  
Jopeth M. Ramis ◽  
Bryan B. Pajarito ◽  
Custer C. Deocaris
Keyword(s):  
Standard Deviation ◽  
Taguchi Method ◽  
Flow Rate ◽  
Fiber Diameter ◽  
Morphological Characteristics ◽  
Polymer Composition ◽  
Solution Flow Rate ◽  
Solvent Ratio ◽  
Average Fiber Diameter ◽  
Fiber Morphology

The study presents a method on producing defect-free polyvinyl alcohol-gelatin (PVAG) nanofibers by considering multiple morphological characteristics of the produced nanofibers using the Taguchi method. Aside from minimizing the average fiber diameter, the method was also used to produce consistent, monodispersed PVAG nanofibers without the usual defects of beading and splattering. The experiments are performed considering the effect of polymer composition (PVAG ratio and solvent ratio of water, formic acid, and acetic acid H2O:FA:HAc) and process factors (tip-to-collector distance TCD and solution flow rate) on fiber morphology. Fiber morphology is measured in terms of 4 responses: average fiber diameter, standard deviation of fiber diameter, occurrence of beading, and occurrence of splattering. Results show that maximum overall desirability for electrospinning PVAG nanofibers at smallest average diameter and deviation (26.10 ± 9.88 nm) with chance of moderate beading and zero splattering is predicted at PVAG mass ratio of 6.5:3.5, H2O:FA:HAc solvent volume ratio of 4:4:2, TCD of 12.5 cm, and flow rate of 1 ml h-1. Results of confirmatory run agree with the predicted factor levels at maximum desirability, with average fiber diameter and standard deviation measured to be 26.95 ± 6.39 nm. PVAG nanofibers of the confirmatory run are also both bead-and splatter-free. Results suggest the application of Taguchi method can offer a robust and rapid approach in deriving the conditions for production of new and high-quality PVAG nanofibers for tissue engineering scaffolds.

Preparation and Characterization of Polyacrylonitrile-Polyaniline Blend Nanofibers

2010 ◽  
Vol 44-47 ◽  
pp. 2195-2198 ◽  
Author(s):  
Wei Pan ◽  
Xiao Wei He ◽  
Yan Chen
Keyword(s):  
Hydrogen Bonding ◽  
Sulfonic Acid ◽  
Composite Nanofibers ◽  
Fiber Diameter ◽  
Electrospinning Process ◽  
Average Fiber Diameter ◽  
Scanning Calorimetry ◽  
Dodecylbenzene Sulfonic Acid ◽  
Scanning Electron

Composite nanofibers of polyaniline doped with dodecylbenzene sulfonic acid (PANI-DBSA) and polyacrylonitrile (PAN) were prepared via an electrospinning process. The surface morphology of PANI-DBSA/PAN nanofibers was characterized using scanning electron microscopy (SEM). Differential scanning calorimetry (DSC) and Fourier transform infrared spectroscopy (FTIR) were used to investigate the hydrogen bonding and miscibility behavior of the blend. It was found that the average fiber diameter of composites nanofiber was increased with the increase of PANI-DBSA content. The formation of the hydrogen bonding between PAN and PANI-DBSA was identified by the FTIR spectra

scholarly journals In Vitro Assessment of Synthetic Nano Engineered Graft Designed for Further Clinical Study in Nerve Regeneration

2018 ◽  
Vol 5 (3) ◽  
pp. 86-91
Author(s):  
Ali Sadeghi ◽  
Fatholah Moztarzadeh ◽  
Jamshid Aghazadeh Mohandesi ◽  
Claudia Grothe ◽  
Kirsten Haastert Talini ◽  
...  
Keyword(s):  
Cell Attachment ◽  
Fiber Diameter ◽  
Solvent System ◽  
Neural Cell ◽  
Neural Regeneration ◽  
Nanofibrous Scaffolds ◽  
Composition Modification ◽  
Chitosan Fiber ◽  
Surface Modified

Background: Electrospun nanofibrous scaffolds are considered as promising candidates in neural tissue regeneration due to their ability to support neural cell attachment, spreading and proliferation. Methods: In this paper, various type of nanofibers scaffold based on polycaprolactone) (PCL) were fabricated using electrospinning. The main drawback of PCL scaffolds is their low bioactivity of scaffold surface. To overcome this surface and composition modification was used to enhanced hydrophilicity and bioactivity of scaffold. Results: The scanning electron microscopy (SEM) results indicate that fiber diameter entirely depends on the solvent system and added component of gelatin and chitosan which by adding gelatin and chitosan fiber diameter decreased. In vitro studies using PC12 cells revealed that the plasma surface modified and blended scaffold with chitosan and gelatin nanofibrous scaffold supports cell attachment, spreading and indicate a significant increase in proliferation of PC12 in the presence of chitosan. The results demonstrated that gelatin and chitosan caused a significant enhancement in the bioactivity of the scaffold, which confirmed by MTT assay and improved the cell spreading and proliferation of neural cell on the scaffolds. Conclusion: Based on the experimental results, the PCL/chitosan/PPy conductive substrate could be used as a potential scaffold for clinical research in the field of neural regeneration and healing.

Release Profile of a Model Protein Drug Depending on the Stability of Microspheres Based on Polyelectrolyte Complex

2007 ◽  
Vol 342-343 ◽  
pp. 505-508
Author(s):  
Sung Won Kim ◽  
Yun Sik Nam ◽  
Yeon Jin Min ◽  
Jong Ho Kim ◽  
Kwang Meyong Kim ◽  
...  
Keyword(s):  
Polyelectrolyte Complex ◽  
Coating Layer ◽  
Great Influence ◽  
Release Profile ◽  
Core Material ◽  
Glycol Chitosan ◽  
The Core ◽  
Key Factor ◽  
Model Protein ◽  
The Stability

Stability and disintegration of natural polyelectrolyte complex microspheres for protein drugs delivery have been extensively investigated because of their great influence on the drug release patterns. In this study, we tested stability of microspheres with alginate (Alg) core layered by either chitosan (Chi) or glycol chitosan (GChi) by examining release profiles of fluorophorelabeled bovine serum albumin (BSA) and lysozyme (Lys) from the microspheres. While GChi shell was disintegrated quickly, Chi-shell microspheres showed good stability in PBS. Disintegration of the coated layer induced the core material instable. The results indicated that while the charges of the shell material provided additional diffusion barrier against the protein release, the key factor to hold the proteins inside the microspheres was the integrity of the outer coating layer.

scholarly journals Electrospinning of Cellulose Nanocrystal-Filled Poly (Vinyl Alcohol) Solutions: Material Property Assessment

Nanomaterials ◽  
2019 ◽  
Vol 9 (5) ◽  
pp. 805 ◽  
Author(s):  
J. Elliott Sanders ◽  
Yousoo Han ◽  
Todd S. Rushing ◽  
Douglas J. Gardner
Keyword(s):  
Vinyl Alcohol ◽  
Fiber Diameter ◽  
Morphological Structure ◽  
Poly Vinyl Alcohol ◽  
Oh Groups ◽  
Heat Treated ◽  
Property Assessment ◽  
Vinyl Group ◽  
Random Composite ◽  
Scanning Electron

Poly (vinyl alcohol) (PVA) and cellulose nanocrystals (CNC) random composite mats were prepared using the electrospinning method. PVA/CNC mats were reinforced with weight concentrations of 0, 20 and 50% CNC (w/w) relative to PVA. Scanning electron microscopy was used to measure the fiber diameter, which ranged from 377 to 416 nm. Thermogravimetric analysis (TGA) confirmed the presence of CNC fibers in the mat fibers which were not visible by scanning electron microscope (SEM). Mechanical testing was conducted using ASTM D 638 on each sample group at 10 mm min−1. Neat PVA and PVA/CNC mats were heat treated at 170 °C for 2h hours, and the morphological structure was maintained with some fiber diameter reduction. Mechanical property results after heat treatment showed a decrease in tensile strength, an increase in tensile stiffness and a decrease in strain to yield (%). This effect was attributable to enhanced diffusion bonding of the mat fiber intersections. The CNC fibers also increased mat stiffness, and reduced strain to yield in non-treated mats. The use of CNCs show potential for compounding into bulk polymer composites as a reinforcement filler, and also show promise for chemical crosslinking attributable to the –OH groups on both the PVA, in addition to esterification of the vinyl group, and CNC.

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