scholarly journals Genetic and Metabolic Intraspecific Biodiversity ofGanoderma lucidum

2015 ◽  
Vol 2015 ◽  
pp. 1-13 ◽  
Author(s):  
Anna Pawlik ◽  
Grzegorz Janusz ◽  
Iwona Dębska ◽  
Marek Siwulski ◽  
Magdalena Frąc ◽  
...  
Keyword(s):  
Ganoderma Lucidum ◽  
Mycelial Growth ◽  
Carbon Sources ◽  
Fungal Growth ◽  
Its Region ◽  
Mitochondrial Activity ◽  
Genomic Relationship ◽  
Profile Similarity ◽  
Geographic Regions ◽  
Its Region Sequencing

FourteenGanoderma lucidumstrains from different geographic regions were identified using ITS region sequencing. Based on the sequences obtained, the genomic relationship between the analyzed strains was determined. AllG. lucidumstrains were also genetically characterized using the AFLP technique.G. lucidumstrains included in the analysis displayed an AFLP profile similarity level in the range from 9.6 to 33.9%. Biolog FF MicroPlates were applied to obtain data on utilization of 95 carbon sources and mitochondrial activity. The analysis allowed comparison of functional diversity of the fungal strains. The substrate utilization profiles for the isolates tested revealed a broad variability within the analyzedG. lucidumspecies and proved to be a good profiling technology for studying the diversity in fungi. Significant differences have been demonstrated in substrate richness values. Interestingly, the analysis of growth and biomass production also differentiated the strains based on the growth rate on the agar and sawdust substrate. In general, the mycelial growth on the sawdust substrate was more balanced and the fastest fungal growth was observed for GRE3 and FCL192.

scholarly journals Optimum vegetative growth conditions and genetic diversity in different strains of Pleurotus salmoneastramineus L.J.N. Vassilzeva

2020 ◽  
Vol 49 (1) ◽  
pp. 125-134
Author(s):  
Nuhu Alam ◽  
Farhana Rahman
Keyword(s):  
Genetic Diversity ◽  
Mycelial Growth ◽  
Culture Media ◽  
Inorganic Nitrogen ◽  
Carbon Sources ◽  
Nitrogen Sources ◽  
Its Region ◽  
Growth Conditions ◽  
Malt Extract ◽  
Arbitrary Primers

This experiment was undertaken to depict the favourable condition for mycelial growth, molecular identification and phylogenetic relationship of the selected strains of Pleurotus salmoneostramineus. Suitable temperature and pH were obtained at 25ºC and 6, respectively. Mushroom complete, glucose peptone and yeast malt extract culture media were favorable, while Hennerberg and Hoppkins were unfavorable. Dextrin was the best and xylose was the less effective carbon sources. Inorganic nitrogen sources were less effective for the mycelial growth of P. salmoneostramineus. The sequences of internal transcribed spacer (ITS) region of selected strains revealed that the total length ranged from 614 to 663 bp. The size of the ITS1 and ITS2 regions varied among the strains. Sequence analysis showed that 5.8S of rDNA sequences were identical. Phylogenetic tree of the ITS region sequences indicated that strains of P. salmoneostramineus belong to same cluster. The reciprocal homologies of the ITS region sequences ranged from 98 to 100%. The strains of P. salmoneostramineus were also analyzed by random amplification of polymorphic DNA (RAPD) with 20 arbitrary primers. RAPD results suggested that tested strains of P. salmoneostramineus were genetically similar with some variations, thus it could be concluded that RAPD and ITS techniques were well competent for detecting the genetic diversity of all tested strains of P. salmoneostramineus.

scholarly journals New Isolated Metschnikowia pulcherrima Strains from Apples for Postharvest Biocontrol of Penicillium expansum and Patulin Accumulation

Toxins ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 397
Author(s):  
Laura Settier-Ramírez ◽  
Gracia López-Carballo ◽  
Pilar Hernández-Muñoz ◽  
Angélique Fontana ◽  
Caroline Strub ◽  
...  
Keyword(s):  
Liquid Medium ◽  
Fungal Growth ◽  
Its Region ◽  
Antagonistic Activity ◽  
Penicillium Expansum ◽  
Metschnikowia Pulcherrima ◽  
Potential Activity ◽  
Different Strains ◽  
Main Producer

Wild yeasts isolated from the surface of apples were screened for antagonistic activity against Penicillium expansum, the main producer of the mycotoxin patulin. Three antagonistic yeasts (Y33, Y29 and Y24) from a total of 90 were found to inhibit P. expansum growth. Identification by ITS region sequence and characterization showed that three selected isolates of yeast should be different strains of Metschnikowia pulcherrima. Several concentrations of the selected yeasts were used to study their in vitro antifungal effectivity against P. expansum on Petri dishes (plates with 63.6 cm2 surface) whereas their potential activity on patulin reduction was studied in liquid medium. Finally, the BCA that had the best in vitro antifungal capacity against P. and the best patulin degradation capacity was selected to be assessed directly on apples. All the selected strains demonstrated antifungal activity in vitro but the most efficient was the strain Y29. Isolated strains were able to reduce patulin content in liquid medium, Y29 being the only strain that completely reduced patulin levels within 120 h. The application of Y29 as biocontrol agent on the surface of apples inoculated with P. expansum, inhibited fungal growth and patulin production during storage. Therefore, the results shown that this yeast strain could be used for the reduction of P. expansum and its mycotoxin in apples or apple-based products by adapting the procedure application.

scholarly journals Fungicidal effect of silver nanoparticles on toxigenic fungi in cocoa

2016 ◽  
Vol 51 (12) ◽  
pp. 1929-1936 ◽  
Author(s):  
Raquel Villamizar-Gallardo ◽  
Johann Faccelo Osma Cruz ◽  
Oscar Orlando Ortíz-Rodriguez
Keyword(s):  
Silver Nanoparticles ◽  
Fusarium Solani ◽  
Theobroma Cacao ◽  
Mycelial Growth ◽  
Culture Media ◽  
Fungal Growth ◽  
Inhibitory Effect ◽  
Plant Tissues ◽  
Inhibition Effect ◽  
Toxigenic Fungi

Abstract: The objective of this work was to evaluate the microbicidal effect of silver nanoparticles (AgNPs) on potentially toxigenic fungi affecting cocoa (Theobroma cacao) crops. These fungi, isolated from diseased cocoa pods, were characterized phenotypically and genotypically. The microbicidal effect was assessed by measuring radial mycelial growth, in synthetic culture media, and at different AgNP concentrations in plant tissues. The inhibition effect was monitored in Petri dishes, and changes in fungal structures were observed through scanning electron microscopy. Two potentially toxigenic fungi were highly prevalent: Aspergillus flavus and Fusarium solani. The inhibition assays, performed in liquid and solid synthetic culture media, showed that AgNPs did not significantly affect the growth of these fungi, even at the highest concentration (100 ppm). By contrast, they showed a positive inhibitory effect in plant tissues, especially in the cortex, when infected with A. flavus, in which an 80 ppm dose completely inhibited fungal growth. However, once fungi have managed to penetrate inside the pods, their growth is unavoidable, and AgNP effect is reduced. On F. solani, the studied nanomaterial only induced some texture and pigmentation changes. The microbicidal effect of chemically synthesized silver nanoparticles is greater in plant tissues than in culture media.

scholarly journals Effect of kebar grass (Biophytum petersianum Klotzsch) leaf extract on the growth and morphological structure of aflatoxigenic Aspergillus flavus

Food Research ◽  
2019 ◽  
Vol 4 (1) ◽  
pp. 234-243
Author(s):  
M.M. Lisangan ◽  
R. Syarief ◽  
W.P. Rahayu ◽  
O.S. Dharmaputra
Keyword(s):  
Aspergillus Flavus ◽  
Mycelial Growth ◽  
Morphological Structure ◽  
Fungal Growth ◽  
Morphological Alterations ◽  
Extract Concentration ◽  
Growth Inhibitory ◽  
Aflatoxigenic Fungi ◽  
Biophytum Petersianum ◽  
Observation Results

The objective of this study was to investigate the antifungal activity of kebar grass (Biophytum petersianum Klotzsch) extract (KGE) on the mycelial growth, conidiation and morphological structure of two types of aflatoxigenic fungus, which are Aspergillus flavus BCCF 0219 and A. flavus BIO 2236. They were isolated in the three types of model media, namely carbohydrate-enriched medium, fat-enriched medium and protein-enriched medium with five concentrations of KGE (12, 14, 16, 18, and 20 mg/mL) on each media. The best extract concentration of that inhibits the growth of A. flavus BCCF 0219 was found in the carbohydrate-enriched medium (95.7%), which was 12 mg/mL, whereas at A. flavus BIO 2236 was found in the fat-enriched medium (100%), which was 16 mg/mL. Based on SEM observation results, it was found that the mechanisms involved in fungal growth inhibitory by the KGE were by morphological alterations of the hyphal development, and the collapse of the entire hyphae. These findings indicated that KGE as a potential natural antifungal agent, particularly against aflatoxigenic fungi.

scholarly journals Selection of filamentous fungi that are resistant to the herbicides atrazine, glyphosate and pendimethalin

2021 ◽  
Vol 43 ◽  
pp. e51656
Author(s):  
Nara Priscila Barbosa Bravim ◽  
Anatércia Ferreira Alves ◽  
José Fábio França Orlanda ◽  
Patricia Barbosa Rodrigues Silva
Keyword(s):  
Filamentous Fungi ◽  
Mycelial Growth ◽  
Culture Medium ◽  
Agricultural Soils ◽  
Culture Media ◽  
Fusarium Verticillioides ◽  
Fungal Growth ◽  
Relative Growth ◽  
Maximum Inhibition ◽  
Selection Of

The objective of the present study was to isolate fungi from agricultural soils and evaluate fungal growth in culture medium contaminated with atrazine, glyphosate and pendimethalin. Filamentous fungi were isolated from agricultural soils and cultured in a modified culture medium containing 0, 10, 20, 50, and 100 μg mL-1 atrazine, glyphosate and pendimethalin for 14 days at 28°C. The fungi that presented optimal and satisfactory growth were plated in Sabouraud culture medium with 4% dextrose and containing the herbicides at concentrations of 0, 10, 20, 50, and 100 μg mL-1 for seven days at 28°C. The mean mycelial growth values were submitted to analysis of variance and the Tukey test (p < 0.05%) for comparison and relative growth determination, and maximum inhibition rates were calculated. The isolated fungi Aspergillus fumigatus, Fusarium verticillioides and Penicillium citrinum were shown to be resistant to atrazine, glyphosate and pendimethalin. F. verticillioides showed higher mean mycelial growth in the culture media contaminated with atrazine and glyphosate than the other two fungi. In the culture medium contaminated with pendimethalin, F. verticillioides, and A. fumigatus presented the highest mean mycelial growth values.

Carbon utilization profiles of Fusarium virguliforme isolates

2010 ◽  
Vol 56 (12) ◽  
pp. 979-986 ◽  
Author(s):  
E. Tang ◽  
C.B. Hill ◽  
G.L. Hartman
Keyword(s):  
Carbon Source ◽  
Mycelial Growth ◽  
Carbon Sources ◽  
Geographic Origin ◽  
Acid Methyl Ester ◽  
Published Data ◽  
Carbon Source Utilization ◽  
Carbon Utilization ◽  
Fusarium Virguliforme ◽  
Carbon Utilization Profiles

Fusarium virguliforme is the cause of sudden death syndrome in soybean. Physiological variability among isolates of the fungus is unknown. One way to measure physiologic variability is to analyze growth on different carbon sources. The carbon source utilization profiles of 18 F. virguliforme isolates were examined using the Biolog FF 96-well microplate, which contains 95 different carbon sources. The utilization of dextrin, d-mannitol, maltotriose, d-lactic acid methyl ester, N-acetyl-d-galactosamine, salicin, d-trehalose, and l-alanine differed significantly among isolates (P = 0.05). Carbon sources were grouped into 3 clusters based on their ability to promote growth of F. virguliforme, after calculating Euclidean distances among them. About 12% of the carbon sources promoted a high amount of mycelial growth, 39% promoted a medium amount of growth, and 49% promoted a low amount of mycelial growth; the latter was not significantly different from the water blank control. A hierarchical tree diagram was produced for the 18 isolates based on their carbon source utilization profiles using Ward’s hierarchical analysis method. Two main clusters of isolates were formed. One cluster represented greater average mycelial growth on all of the carbon sources than the other cluster. In this study, variability in carbon source utilization among F. virguliforme isolates was evident, but the results were not associated with geographic origin of the isolates, year collected, or published data on aggressiveness. Additional research is needed to determine if these carbon utilization profiles are associated with other biological characteristics, like spore germination, propagule formation, and saprophytic competitiveness.

scholarly journals In vitro fungitoxic potential of Lippia gracilis (Schauer) essential oil against phytopathogens

2020 ◽  
pp. 667-674
Author(s):  
Kevison Romulo da Silva França ◽  
Flavia Mota de Figueredo Alves ◽  
Tiago Silva Lima ◽  
Alda Leaby dos Santos Xavier ◽  
Plínio Tércio Medeiros de Azevedo ◽  
...  
Keyword(s):  
Essential Oil ◽  
Mycelial Growth ◽  
Fungal Growth ◽  
Fungal Species ◽  
Phytopathogenic Fungi ◽  
Negative Control ◽  
Growth Speed ◽  
Randomized Design

This study evaluates the in vitro effects of Lippia gracilis essential oil on the mycelial growth of phytopathogenic fungi. Experiments were carried out using a completely randomized design to assess the effects of eight treatments. Five replicates were evaluated for each experimental group. The essential oil was incorporated into the potato dextrose culture medium and poured into Petri dishes. Treatments were comprised of different concentrations of the oil (0.0125, 0.025, 0.05, 0.1, and 0.2%), a negative control (0.0%), and two positive controls (commercial fungicides). The plates were inoculated with fungi including Colletotrichum gloeosporioides, C. musae, C. fructicola, C. asianum, Alternaria alternata, A. brassicicola, Fusarium solani, F. oxysporum f. sp. cubense, and Lasiodiplodia theobromae and were incubated for seven days at 27 ± 2°C. The following variables were measured to verify the differences observed among treatments: percentage of mycelial growth inhibition and index of mycelial growth speed. All concentrations of L. gracilis oil inhibited the mycelial growth of the fungal species evaluated. The complete inhibition was observed between concentrations of 0.0125 and 0.1%. Treatment with oil inhibited fungal growth with similar, or even greater, efficiency than commercial fungicides.. We recommend the development of in vivo tests to verify whether L. gracilis essential oil can protect against fungal disease in live plants.

Ethylene promotes mycelial growth and ganoderic acid biosynthesis in Ganoderma lucidum

2016 ◽  
Vol 39 (2) ◽  
pp. 269-275 ◽  
Author(s):  
Guang Zhang ◽  
Ang Ren ◽  
Fengli Wu ◽  
Hanshou Yu ◽  
Liang Shi ◽  
...  
Keyword(s):  
Ganoderma Lucidum ◽  
Mycelial Growth ◽  
Acid Biosynthesis ◽  
Ganoderic Acid
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