scholarly journals Reduced-Pressure Boiling Extraction of Oleuropein Coupled with Ultrasonication from Olive Leaves (Olea europaeaL.)

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Pu-jun Xie ◽  
Li-xin Huang ◽  
Cai-hong Zhang ◽  
Feng You ◽  
Cheng-zhang Wang ◽  
...  
Keyword(s):  
Ethanol Concentration ◽  
Extraction Yield ◽  
Optimal Operation ◽  
Ultrasonic Power ◽  
Extraction Temperature ◽  
Optimal Conditions ◽  
Olive Leaves ◽  
Sem Images ◽  
Reduced Pressure ◽  
Operation Conditions

Oleuropein was extracted fromFrantoioolive leaves using reduced-pressure boiling extraction coupled with ultrasound-assist (URPE). Four important factors, extraction temperature, ultrasonic power, ethanol concentration, and the ratio of solid to liquid, were selected to carry out the response surface methodology (RSM) for seeking optimal conditions on high oleuropein extraction yield at different levels. Box-Behnken design was employed to investigate the effects of the four factors on it. The results showed that the ratio of solid to liquid was the most significant factor of all on oleuropein yield. The optimal operation conditions were obtained as follows: ethanol concentration 75% (v/v), extraction temperature 53°C, ultrasonic power 600 W, and the ratio of solid to liquid 1 : 31. Under these optimal conditions, oleuropein extraction yield was 7.08%, which was close to the predicted value 7.121%. The scanning electron microscope (SEM) images of olive leaves after extraction were provided as well. It was seen that, compared with the untreated leaves, URPE could effectively break cells within the olive leaves.

Study on the Optimization of Extraction Technology of Flavonoids in Clerodendrum inerme(L.) Gaertn. Roots

2012 ◽  
Vol 560-561 ◽  
pp. 349-354
Author(s):  
Jing Xu ◽  
Chuan Bo Cao ◽  
Dong Hai Li ◽  
Yan Feng Liu ◽  
Zhen Yi Liang ◽  
...  
Keyword(s):  
Ethanol Concentration ◽  
Extraction Yield ◽  
Extraction Time ◽  
Optimum Operating ◽  
Extraction Temperature ◽  
Liquid Ratio ◽  
Extraction Technology ◽  
Mangrove Plant ◽  
Operation Conditions ◽  
Total Flavonoids Content

Abstract. Based on the results of single factor experiments on the extraction of total flavonoids content (TFC) in roots of semi-mangrove plant Clerodendrum inerme(L.) Gaertn., operation conditions were optimized with orthogonal methodology. Four factors were applied to evaluate the extraction yield, including the ethanol concentration, extraction time, temperature and solid to liquid ratio. According to the experimental results, the influence order of different factors on the yield of TFC from the roots: ethanol concentration > solid to liquid ratio > extraction temperature > extraction time. The optimum operating parameters were determined as following, ethanol concentration of 70.0 %, extraction time of 90.0 min, temperature of 60.0 °C and solid to liquid ratio of 1:30. Under the optimized conditions, the maximal yield of TFC was enhanced to 1.279%.

scholarly journals Effect of Time, Solvent-Solid Ratio, Ethanol Concentration and Temperature on Extraction Yield of Phenolic Compounds From Olive Leaves

2018 ◽  
Vol 8 (2) ◽  
pp. 2805-2808
Author(s):  
N. Elboughdiri
Keyword(s):  
Phenolic Compounds ◽  
Ethanol Concentration ◽  
Extraction Yield ◽  
Extraction Time ◽  
Total Phenolic ◽  
Optimal Conditions ◽  
Total Phenolic Compounds ◽  
Olive Leaves ◽  
Factors Affecting ◽  
Solid Ratio

This study aims to determine the factors affecting the process of extraction of phenolic compounds from olive leaves. Two methods of extraction were used in this work and different tests were implemented with the aim of optimizing the recovery of phenolic compounds from olive leaves. The factors considered were the extraction time, temperature, solvent-solid ratio and the ethanol concentration. Obtained results show that the recommended optimal conditions for the extraction of the total phenolic compounds from olive leaves were found at 40°C with a solvent/solid ratio of 30:1 and ethanol concentration of 80% (v/v) when the dried olive leaves are stored at -21°C at the beginning of the experiments.

scholarly journals Optimized extraction of polyphenols from leaves of Rosemary (Rosmarinus officinalis L.) grown in Lam Dong province, Vietnam, and evaluation of their antioxidant capacity

2021 ◽  
Vol 19 (1) ◽  
pp. 1043-1051
Author(s):  
Minh-Tam Nguyen-Kim ◽  
Quoc-Cuong Truong ◽  
Minh-Thuy Nguyen ◽  
Bich-Hang Cao-Thi ◽  
Thanh-Danh Tong ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Ethanol Concentration ◽  
Extraction Yield ◽  
Food Preservation ◽  
Solvent Ratio ◽  
Extraction Temperature ◽  
Optimal Conditions ◽  
Polyphenol Content ◽  
Feed Material ◽  
Dried Extract

Abstract In the present study, the optimized solvent extraction conditions with regards to the total polyphenol content (TPC) and antioxidant capacity of rosemary leaf extract (RLE) were determined. The one-factor-at-a-time method was used to independently investigate the effect of several extraction parameters, including ethanol concentration (0–100% v/v), extraction temperature (50–80°C), extraction period (15–60 min), material–solvent ratio (1:5–1:10 g/mL), and extraction cycles (1, 2, and 3 times) on polyphenol content. Response surface methodology (RSM), in combination with a central composite design, was used to perform optimization. The following optimal conditions that gave maximal TPC were determined and experimentally verified: ethanol concentration of 65% (v/v), extraction temperature of 65°C, material–solvent ratio of 1:7.5 g/mL, extraction time of 15 min, and 2 cycles of extraction. These parameters corresponded with the TPC yield of 87.42 ± 0.25 mg gallic acid equivalent/g dried feed material (mg GAE/g DW). The optimal conditions gave a high extraction yield (337 ± 6 mg dried extract/g dried feed material) with 197.28 ± 3.11 mg GAE/g dried extract. The estimated models were strongly significant (p < 0.05) for TPC values with significant regression coefficients (R 2) of 0.9979. The obtained RLE was supposed to be the top grade of natural antioxidant with the IC50 (DPPH assays) value of 9.4 ± 0.1 μg/mL, which is higher than that of the vitamin C by just three times (IC50 = 3.2 ± 0.1 μg/mL). Current results justify RLE as a potential agent in food preservation applications.

scholarly journals Homogenate Extraction of Crocins from Saffron Optimized by Response Surface Methodology

2018 ◽  
Vol 2018 ◽  
pp. 1-6 ◽  
Author(s):  
Yingpeng Tong ◽  
Yu Jiang ◽  
Dan Guo ◽  
Yongqiu Yan ◽  
Shiping Jiang ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Ethanol Concentration ◽  
Extraction Yield ◽  
Extraction Time ◽  
Process Conditions ◽  
Extraction Temperature ◽  
Single Factor ◽  
Extraction Voltage ◽  
Homogenate Extraction

Saffron, which has many kinds of biological activities, has been widely used in medicine, cosmetics, food, and other fields of health promotion industries. Crocins are the main component of saffron (Crocus sativus L.). At present, most of the extraction methods for crocins require long time or special instruments to complete the process and some of them are not suitable for industrial production at present. In this article, homogenate extraction technology which is a convenient and efficient method was developed for crocins extraction from saffron. Firstly, the influences of extraction voltage, extraction time, ethanol concentration, and temperature on crocins yield were studied by single factor experiments; and then response surface methodology (RSM) was used to optimize levels of four variables based on the result of single factor experiments. Results showed that the optimum extraction process conditions for crocins were as follows: extraction voltage, 110 V; ethanol concentration, 70%; extraction temperature, 57°C; and extraction time, 40 s. Based on these conditions, the extraction yield of crocins can reach 22.76% which is higher than ultrasonic extraction method. Therefore, homogenate extraction is an effective way to extract crocins from saffron with higher extraction yield and shorter extraction time.

Optimization of the Ultrasonic Extraction of Gypenoside III from Gynostemma pentaphyllum by Response Surface Methodology

2012 ◽  
Vol 550-553 ◽  
pp. 1866-1870
Author(s):  
Xiao Dan Tang ◽  
Hai Yang Hang ◽  
Shao Yan Wang ◽  
Jing Xiang Cong
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Extraction Yield ◽  
Raw Material ◽  
Extraction Temperature ◽  
Optimal Conditions ◽  
Gynostemma Pentaphyllum ◽  
Bioactive Component ◽  
Yield Value ◽  
Ultrasonic Time

Gypenosides III is a major bioactive component which is rich in Gynostemma pentaphyllum. For better utilization of the native resource, response surface methodology was used to optimize the extraction conditions of gypenosides III from G. pentaphyllum. The effects of three independent variables on the extraction yield of gypenosides III were investigated and the optimal conditions were evaluated by means of Box-Behnken design. The optimal conditions are as follows: ratio of ethanol to raw material 25, extraction temperature 58°C and ultrasonic time 25min. Under these conditions, the yield of gypenoside III is 1.216±0.05%, which is agreed closely with the predicted yield value.

scholarly journals The extraction of total lipids from parsley: Petroselinum crispum (mill.) Nym. Ex. A.W. Hill) seeds

2004 ◽  
Vol 58 (12) ◽  
pp. 563-568 ◽  
Author(s):  
Mihajlo Stankovic ◽  
Nadica Stojanovic ◽  
Nada Nikolic ◽  
Vesna Novkovic
Keyword(s):  
Kinetic Equations ◽  
Optimal Operation ◽  
Petroselinum Crispum ◽  
Optimal Conditions ◽  
Total Lipids ◽  
Operation Conditions ◽  
Lipid Fractions ◽  
Polar Organic Solvents ◽  
Layer Chromatography ◽  
Kinetics Of

The kinetics of extraction of total lipids from ground parsley (Petroselinum crispum (Mill.) Nym. ex. A.W. Hill) seeds with a mixture of ethanol or methanol with non-polar organic solvents, chloroform, carbon tetrachloride, trichloroethylene and petroleum ether, at various temperatures were studied. The maceration technique with reflux was used. The kinetic parameters were determined in extraction kinetic equations, as well as the optimal operation conditions for total lipids extraction. The maximum total lipids yield under optimal conditions was 33.7 g per 100 g of dry parsley seeds. Nine lipid fractions of the total lipids were separated by thin layer chromatography among which were phospholipids, sterol, mono-, di- and triacylglycerol, free fatty acids and carbohydrates.

scholarly journals Effects of Microwave-Assisted Extraction Conditions on Antioxidant Capacity of Sweet Tea (Lithocarpus polystachyus Rehd.)

Antioxidants ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 678
Author(s):  
Ao Shang ◽  
Min Luo ◽  
Ren-You Gan ◽  
Xiao-Yu Xu ◽  
Yu Xia ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Microwave Power ◽  
Ethanol Concentration ◽  
Extraction Time ◽  
Extraction Temperature ◽  
Microwave Assisted Extraction ◽  
Optimal Conditions ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Sample Ratio

In this study, the effects of microwave-assisted extraction conditions on antioxidant capacity of sweet tea (Lithocarpus polystachyus Rehd.) were studied and the antioxidants in the extract were identified. The influences of ethanol concentration, solvent-to-sample ratio, microwave power, extraction temperature and extraction time on Trolox equivalent antioxidant capacity (TEAC) value, ferric reducing antioxidant power (FRAP) value and total phenolic content (TPC) were investigated by single-factor experiments. The response surface methodology (RSM) was used to study the interaction of three parameters which had significant influences on antioxidant capacity including ethanol concentration, solvent-to-sample ratio and extraction time. The optimal conditions for the extraction of antioxidants from sweet tea were found as follows—ethanol concentration of 58.43% (v/v), solvent-to-sample ratio of 35.39:1 mL/g, extraction time of 25.26 min, extraction temperature of 50 ℃ and microwave power of 600 W. The FRAP, TEAC and TPC values of the extract under the optimal conditions were 381.29 ± 4.42 μM Fe(II)/g dry weight (DW), 613.11 ± 9.32 μM Trolox/g DW and 135.94 ± 0.52 mg gallic acid equivalent (GAE)/g DW, respectively. In addition, the major antioxidant components in the extract were detected by high-performance liquid chromatography with diode array detection (HPLC-DAD), including phlorizin, phloretin and trilobatin. The crude extract could be used as food additives or developed into functional food for the prevention and management of oxidative stress-related diseases.

scholarly journals Optimisation of antioxidant extraction from lemon balm (Melissa officinalis)

2012 ◽  
Vol 30 (No. 4) ◽  
pp. 385-393 ◽  
Author(s):  
M. Ondrejovič ◽  
F. Kraic ◽  
H. Benkovičová ◽  
S. Šilhár
Keyword(s):  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Beta Carotene ◽  
Extraction Yield ◽  
Extraction Temperature ◽  
Optimal Conditions ◽  
Total Polyphenol ◽  
Lemon Balm ◽  
Extraction Solvent ◽  
Experimental Values

The effects of the propan-2-ol proportion in the extraction solvent (PPES), solid to liquid ratio (SLR), and extraction temperature on the extraction yield of antioxidants measured by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging activity and &beta;-carotene-linoleic acid bleaching inhibition activity (BCLM) were evaluated. Secondly, total polyphenol and flavonoid contents were determined to find possible relations of these parameters with antioxidant activity. The optimal conditions for the extraction were determined using response surface methodology (RSM). The optimal conditions for the extraction of antioxidants measured by radical scavening activity (DPPH) were PPES 50.2% (v/v), 33.8&deg;C, and SLR 1:147 (w/v). The optimal conditions for the extraction of antioxidants measured by BCLM were PPES 1.15% (v/v), 61.8&deg;C, and SLR 1:153 (w/v). The optimal conditions for the extraction of total polyphenols and total flavonoids were 23.3% (v/v) (PPES), 67.5&deg;C, 1:148 (w/v) (SLR); 1.15% (v/v) (PPES), 80&deg;C, 1:179 (w/v) (SLR); respectively. The experimental values agreed with the predicted ones within a&nbsp;95% confidence interval. &nbsp; &nbsp;

Optimum Process for Extraction of Total Flavonoids from Castor Leaves by Orthogonal Test

2014 ◽  
Vol 989-994 ◽  
pp. 1029-1032
Author(s):  
Yi Hua Jiang ◽  
Xin Long Jiang ◽  
Cheng Gang Cai
Keyword(s):  
Ethanol Concentration ◽  
Orthogonal Test ◽  
Optimum Process ◽  
Total Flavonoids ◽  
Extraction Temperature ◽  
Optimal Conditions ◽  
Liquid Ratio ◽  
Test Methodology ◽  
Maximal Yield ◽  
Solid Liquid

Optimization of total flavonoid extraction from Castor leaves using orthogonal test methodology with the factors of ethanol concentration, extraction temperature,extraction time,solid/liquid ratio on the yield of total flavonoids were investigated.The results showed that optimum extracting conditions of total flavonoids from castor leaves were as follows: adding 1:40 g·mL-1 of 30% alcohol and extracting for 30 min,extraction temperature 70°C. Under the optimal conditions, the maximal yield of flavonoids was of 1.81%.

Study on Subcritical Water Extraction of Flavonoids from Scutellaria baicalenses

2013 ◽  
Vol 662 ◽  
pp. 293-296 ◽  
Author(s):  
Yan Cheng ◽  
Jing Liu ◽  
Chang An Liu
Keyword(s):  
Subcritical Water ◽  
Extraction Yield ◽  
Water Extraction ◽  
Extraction Time ◽  
Particle Sizes ◽  
Extraction Temperature ◽  
Subcritical Water Extraction ◽  
Operation Conditions ◽  
Conventional Extraction ◽  
Total Extraction

The extraction of baicalin, baicalein, wogonin and wogonoside from Scutellaria Baicalenses was performed using subcritical water. The effects of key operation conditions by varying the temperature (110-160 °C), extraction time (10-90 min), water loadings (2.0-4.0 mL)and particle sizes (>20- <100 mesh) were evaluated. The highest extraction yields of baicalin and wogonoside were obtained at extraction temperature of 160 °C, extraction time of 60 min, water loadings of 4.0 mL and 60-100 mesh. The highest extraction yields of baicalein and wogonin were obtained at extraction temperature of 110 °C, extraction time of 10 min, water loadings of 4.0 mL and 60-100 mesh. The subcritical water extraction was compared with the conventional extraction method. The total extraction yield by SCWE was higher than those obtained using ethanol as the solvent.

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